河北医科大学学报 ›› 2021, Vol. 42 ›› Issue (8): 881-885.doi: 10.3969/j.issn.1007-3205.2021.08.004

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支气管哮喘患者气道炎症与EOS的PI3K/Akt信号通路活化程度的关系研究

  

  1. 河北省邢台市第三医院呼吸科，河北 邢台 054000
  • 出版日期:2021-08-25 发布日期:2021-08-27
  • 作者简介:刘红红（1984-），女，河北衡水人，河北省邢台市第三医院主治医师，医学硕士，从事呼吸科疾病诊治研究。
  • 基金资助:
    邢台市科技计划项目（2019ZC208）

Relationship between airway inflammation and PI3K/Akt signaling pathway activation of EOS in patients with bronchial asthma

  1. Department of Respiratory Medicine， the Third Hospital of Xingtai City， Hebei Province， Xingtai 054000， China
  • Online:2021-08-25 Published:2021-08-27

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摘要： 目的  研究支气管哮喘患者气道炎症与嗜酸粒细胞(eosinnophil, EOS)的磷脂酰肌醇激酶3/蛋白激酶B（phosphatidylinositol kinase 3/protein kinase B, PI3K/Akt）信号通路活化程度的关系。
方法  选取于我院就诊的36例气管哮喘患者作为观察组，选取同时段于我院的36例健康体检者为对照组。收集患者临床资料，并检测气道壁面积占气道总横截面积百分比(WA%)、支气管管壁厚度与外径比值(T/D)，以及诱导痰的细胞分布情况。分析不同痰嗜酸粒细胞水平患者的气道重塑、炎症指标以及PI3K/Akt信号通路相关蛋白水平，以及采用Person相关性分析炎症指标与PI3K/Akt信号通路相关蛋白的关系。
结果  观察组嗜酸粒细胞占比均明显高于对照组，而中性粒细胞、巨噬细胞占比明显低于对照组（P＜0.05）。高嗜酸粒细胞组的呼出气一氧化氮(fractional exhaled nitric oxide,FeNO)体积浓度、血清总IgE、T/D、WA明显高于低嗜酸粒细胞组（P＜0.05）。高嗜酸粒细胞组患者的PI3K、p-Akt表达水平明显高于低嗜酸粒细胞组（P＜0.05）。Pearson分析显示FeNO体积浓度、血清总IgE分别与PI3K、p-Akt具有正相关性（P＜0.05）。
结论  支气管哮喘患者的EOS的PI3K/Akt信号通路处于活化状态，与气道炎症（FeNO、血清IgE）具有正相关性。


关键词: 支气管哮喘, 嗜酸粒细胞, 气道炎症

Abstract: Objective  To explore the relationship between airway inflammation and  phosphatidylinositol kinase 3/Protein Kinase B(PI3K/Akt) signaling pathway of activation eosinnophil(EOS) in patients with bronchial asthma. 
Methods  A total of 36 patients with bronchial asthma treated in our hospital were selected as observation group, and 36 people undergoing physical examination during the same period were selected as control group. The clinical data of the patients were collected, and the percentage of bronchial wall area to total airway cross-sectional area(WA%), the ratio of bronchial wall thickness to bronchial lumen diameter(T/D) and the distribution of cells in induced sputum were detected. The airway remodeling, inflammatory indicators, and PI3K/Akt signaling pathway-related protein levels in patients with different sputum eosinophil levels were analyzed, and Person correlation was used to analyze the relationship between inflammatory indicators and PI3K/Akt signaling pathway-related proteins. 
Results  The proportion of eosinophils in the observation group was significantly higher than that in the control group, while the proportion of neutrophils and macrophages in the observation group was significantly lower than that in the control group(P＜0.05). The fractional exhaled nitric oxide(FeNO) volume concentration, serum total IgE, T/D and WA in the high eosinophil group were significantly higher than those in the low eosinophil group(P＜0.05). The PI3K and p-Akt expression level of the patients in the high eosinophil group was significantly higher than that in the low eosinophil group(P＜0.05). Pearson analysis showed that FeNO volume concentration and serum total IgE were positively correlated with PI3K and p-Akt respectively(P＜0.05).  
Conclusion  The PI3K/Akt signaling pathway of EOS in patients with bronchial asthma is activated and has a positive correlation with airway inflammation(FeNO and serum IgE).


Key words: bronchial asthma, eosinophil, inflammation

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