河北医科大学学报 ›› 2023, Vol. 44 ›› Issue (2): 173-178.doi: 10.3969/j.issn.1007-3205.2023.02.010

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LncRNA-MAFG-AS1在结直肠癌中的表达及临床意义

  

  1. 河北医科大学第二医院普外三科，河北 石家庄 050000

  • 出版日期:2023-02-25 发布日期:2023-02-28
  • 作者简介:郝英豪（1982-）， 男，河北邢台人，河北医科大学第二医院主治医师，医学硕士，从事普外科疾病诊治研究。
  • 基金资助:
    河北省医学科学研究课题计划(20211335)

Expression and clinical significance of LncRNA-MAFG-AS1 in colorectal cancer

  1. The Third Department of General Surgery, the Second Hospital of Hebei Medical University， Shijiazhuang 050000, China

  • Online:2023-02-25 Published:2023-02-28

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摘要： 目的 探讨LncRNA-MAFG-AS1在结直肠癌中的表达及临床意义。
方法  选取我院行手术切除的结直肠癌组织及对应的癌旁正常组织各80例，应用定量即时聚合酶链锁反应（quantitative real time polymerase chain reaction，qRT-PCR）检测结直肠癌组织及癌旁正常组织中LncRNA-MAFG-AS1的表达水平，并分析其与结直肠癌临床病理特征之间的关系，所有患者随访截止日期到2019 年12月31日，应用Kaplan-Meier(K-M)法进行生存分析，采用Cox回归模型分析结直肠癌预后的危险因素。
结果 结直肠癌组织中LncRNA-MAFG-AS1相对表达量为2.398±0.214，高于癌旁正常组织的1.032±0.132，差异有统计学意义（P＜0.05）。当患者TNM分期更高、中高分化及存在淋巴结转移和远期转移时，LncRNA-MAFG-AS1过表达(P＜0.05)。K-M生存分析显示高表达患者无进展生存时间(progression-free survival，PFS)为(20.36±3.21)个月，低表达患者为(29.71±4.23)个月；高表达患者的总生存时间(overall survival，OS)为(38.69±6.24)个月，低高表达患者为(54.26±5.14)个月；两组患者的PFS和OS差异有统计学意义(P＜0.05)。Cox多因素回归分析显示，TNM分期更高、中高分化及存在淋巴结转移、远期转移以及LncRNA-MAFG-AS1高表达是影响结直肠癌预后的独立危险因素。
结论 LncRNA-MAFG-AS1在结直肠癌组织中呈高表达，且与结直肠癌患者分化程度、TNM分期、淋巴结和远期转移有关，是影响结直肠癌患者预后的独立风险因素。


关键词: 结直肠肿瘤, LncRNA-MAFG-AS1, 预后

Abstract: Objective To investigate the expression and clinical significance of LncRNA-MAFG-AS1 in colorectal cancer (CRC). 
Methods A total of 80 cases of CRC tissues that were surgically removed in our hospital and corresponding adjacent normal tissues were selected. The expression levels of LncRNA-MAFG-AS1 in CRC tissues and adjacent normal tissues were detected by qRT-PCR, and their relationship with the clinicopathological features of CRC was analyzed. All patients were followed up until December 31, 2019. Kaplan-Meier (K-M) method was used for survival analysis, and Cox regression model was used for risk factor analysis of the prognosis of CRC.  
Results The relative expression of LncRNA-MAFG-AS1 in CRC tissues was 2.398±0.214, which was higher than that in adjacent normal tissues 1.032±0.132, with a statistical difference (P＜0.05). The overexpression of LncRNA-MAFG-AS1 was associated with higher TNM stage, moderate and high differentiation, and the presence of lymph node metastasis and long-term metastasis (P＜0.05). K-M survival analysis showed that the progression-free survival (PFS) was (20.36±3.21) months in patients with high expression, and (29.71±4.23) months in patients with low expression; the overall survival (OS) was (38.69±6.24) months in patients with high expression, and (54.26±5.14) months in patients with low and high expression; the difference in PFS and OS between two groups was statistically significant (P＜0.05). Cox multivariate regression analysis showed that higher TNM stage, moderate and high differentiation, presence of lymph node metastasis, long-term metastasis and high expression of LncRNA-MAFG-AS1 were independent risk factors for the prognosis of CRC. 
Conclusion LncRNA-MAFG-AS1 is highly expressed in CRC tissue, and is related to the degree of differentiation, TNM stage, lymph node metastasis and long-term metastasis of CRC patients, which are independent risk factors affecting the prognosis of CRC patients. 


Key words: colorectal neoplasms, LncRNA-MAFG-AS1, prognosis

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