河北医科大学学报 ›› 2023, Vol. 44 ›› Issue (8): 894-899.doi: 10.3969/j.issn.1007-3205.2023.08.005

• • 上一篇    下一篇

能量代谢通路TNAP在2型糖尿病患者的表达及对脂肪细胞功能调控的机制研究

  

  1. 河北省保定市第一中心医院内分泌二科，河北 保定 071051

  • 出版日期:2023-08-25 发布日期:2023-08-28
  • 作者简介:李芳（1989-），女，河北保定人，河北省保定市第一中心医院主治医师，医学硕士，从事内分泌疾病诊治研究。
  • 基金资助:
    保定市科技计划项目（2041ZF076）

Study on the expression of energy metabolism pathway TNAP in patients with type 2 diabetes and its mechanism of regulating adipocyte function

  1. Department of Endocrinology, the First Central Hospital of Baoding City, Hebei Province, Baoding  071051, China
  • Online:2023-08-25 Published:2023-08-28

HTML

PDF (PC)

168

摘要： 目的 探究碱性磷酸酶（alkaline phosphatase，TNAP）在2型糖尿病患者的表达情况及对脂肪细胞新型功能通路非偶联蛋白1（uncoupling protein 1，UCP1）/鸢尾素（Irisin）调控的机制。
方法 队列试验：选取河北省保定市第一中心医院门诊及住院的2型糖尿病患者80例，并收集健康志愿者80例作为对照，通过ELISA法检测两组人群血清TNAP和能量代谢分子UCP1和鸢Irisin表达水平。细胞学实验：将脂肪细胞3T3-L1分为3组，对照组、NC组（转染NC质粒）、和试验组（转染si-TNAP质粒），诱导3T3-L1成脂肪定向分化，油红O染色检测各组细胞成脂肪分化能力，检测各组细胞葡萄糖消耗水平和三酰甘油消耗水平，通过qPCR法能量代谢通路分子UCP1和Irisin mRNA表达水平，Western blot法检测UCP1和Irisin蛋白表达水平。
结果 队列试验：与对照组相比，试验组患者血清TNAP表达水平升高，UCP1和Irisin表达水平显著降低（P＜0.001）；细胞学实验：与对照组相比：试验组细胞成脂肪能力增加；葡萄糖消耗和三酰甘油产生能力显著增加（P＜0.001）；qPCR结果表明与对照组相比，试验组细胞UCP1和Irisin mRNA表达水平升高（P＜0.001），Western blot实验表明结果表明与对照组相比，试验组细胞UCP1和Irisin蛋白表达水平升高（P＜0.001）。
结论  TNAP可能通过抑制能量代谢通路UCP1/Irisin影响脂肪细胞的能量代谢，并参与2型糖尿病的发生发展过程。


关键词: 糖尿病, 2型, 能量代谢, 脂肪细胞

Abstract: Objective To investigate the expression of alkaline phosphatase (TNAP) in patients with type 2 diabetes mellitus (T2DM) and its mechanism of regulating uncoupling protein 1 (UCP1) / Irisin in adipocytes. 
Methods A cohort study was performed on 80 outpatients and inpatients with T2DM in the First Central Hospital of Baoding City, and 80 healthy volunteers were collected as controls. The expression levels of serum TNAP and energy metabolism molecules UCP1 and Irisin in the two groups were detected by enzyme linked immunosorbent assay （ELISA）. In terms of cytological test, 3T3-L1 adipocytes were divided into three groups: control group, NC group (transfected with NC plasmid) and experimental group (transfected with Si TNAP plasmid), and 3T3-L1 adipogenic differentiation was induced. The adipogenic differentiation ability of cells in each group was detected by oil red O staining, and the glucose consumption level and triglyceride consumption level of cells in each group were detected. The expression levels of energy metabolism pathway molecule UCP1 and Irisin mRNA were detected by qPCR, and the protein expression levels of UCP1 and Irisin were detected by Western blot. 
Results For cohort study, compared with the control group, the expression level of serum TNAP in the experimental group increased, while the expression levels of UCP1 and Irisin decreased significantly (P＜0.01). Cytological test showed that compared with the control group, the adipogenic capacity of cells in the experimental group increased, and glucose consumption and triglyceride production increased significantly (P＜0.01). qPCR results showed that compared with the control group, the expression levels of UCP1 and Irisin mRNA in the experimental group increased (P＜0.01), and Western blot results showed that the expression levels of UCP1 and Irisin protein in the experimental group increased (P＜0.01). 
Conclusion TNAP may affect the energy metabolism of adipocytes by inhibiting the energy metabolism pathway UCP1/Irisin, and participate in the occurrence and development of T2DM.


Key words: diabetes, type 2, energy metabolism, adipocytes

版权所有 © 《河北医科大学学报》编辑部
本系统由北京玛格泰克科技发展有限公司设计开发