老年阿尔茨海默病患者血清中TNF-α、M-CSF及miR-181a的表达水平及临床应用价值

doi:10.3969/j.issn.1007-3205.2023.09.003

河北医科大学学报 ›› 2023, Vol. 44 ›› Issue (9): 1006-1010,1026.doi: 10.3969/j.issn.1007-3205.2023.09.003

老年阿尔茨海默病患者血清中TNF-α、M-CSF及miR-181a的表达水平及临床应用价值

1.江苏省徐州市东方人民医院老年精神科，江苏徐州 221000；2.江苏省徐州市东方人民医院
心理科，江苏徐州 221000；3.江苏省徐州市东方人民医院精神科，江苏徐州 221000

出版日期:2023-09-25 发布日期:2023-10-12
作者简介:付丹（1981-），女，黑龙江克东人，江苏省徐州市东方人民医院副主任医师，医学学士，从事老年痴呆疾病诊治研究。
基金资助:
徐州市卫生健康委科技项目（XWKYHT20210553）

Expression levels and clinical application value of TNF-α, M-CSF and miR-181a in serum of elderly AD patients

1.Department of Geriatric Psychiatry, Dongfang People′s Hospital, Jiangsu Province,
Xuzhou 221000, China; 2.Department of Psychology, Dongfang People′s Hospital,
Jiangsu Province, Xuzhou 221000, China; 3.Department of Psychiatry, Dongfang
People′s Hospital, Jiangsu Province, Xuzhou 221000, China

Online:2023-09-25 Published:2023-10-12

摘要/Abstract

摘要： 目的观察阿尔茨海默病(Alzheimer′s disease，AD)患者血清肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）、巨噬细胞集落刺激因子(macrophage colony stimulating factor，M-CSF)及微小RNA（microRNA, miR）-181a表达及诊断价值。
方法选取我院诊治的AD患者140例为研究对象（AD组），根据临床痴呆量表评分分为轻度组52例，中度组49例，重度组39例，以同期健康查体的50例健康人为对照组。应用实时荧光定量聚合酶链式反应（polymerase chain reaction, PCR）法检测血清miR-181a相对表达量,采用酶联免疫吸附实验检测血清TNF-α、MCSF水平。血清TNF-α、M-CSF及miR-181a的相关性采用Pearson相关分析。受试者工作曲线分析血清TNF-α、M-CSF及miR-181a表达对AD的诊断价值。
结果 AD组血清TNF-α、M-CSF及miR181a表达水平均明显高于对照组(t=49.291、30.485、28.313，P＜0.05)。轻、中、重度组间血清TNF-α、M-CSF及miR-181a表达差异有统计学意义（P＜0.05）。AD患者血清TNF-α与M-CSF、miR-181a表达呈显著正相关（r=0.524、0.610，P＜0.05），M-CSF与miR-181a表达呈显著正相关（r=0.498,P＜0.05）。血清TNF-α＞27.18 ng/L、血清M-CSF＞635.81 ng/L及血清miR-181a＞1.83为影响AD发生的危险因素。血清TNF-α、M-CSF、miR-181a及联合检测的曲线下面积分别为0.741(95%CI:0.684~0.789)、0.693(95%CI:0.630~0.757)、0.727(95%CI:0.682~0.774)及0.862(95%CI:0.818~0.899)，联合检测诊断AD的曲线下面积大于TNF-α、M-CSF及miR-181a单独诊断(Z=3.357、5.894、4.911，P＜0.05)。
结论 AD患者血清TNF-α、M-CSF及miR-181a表达升高，三者升高程度是AD发生的独立危险因素，三者联合检测对AD的诊断具有较高的价值。

关键词: 阿尔茨海默病, 肿瘤坏死因子α, 巨噬细胞集落刺激因子

Abstract: Objective To observe the expression of tumor necrosis factor-α (TNF-α), macrophage colony stimulating factor (M-CSF) and microRNA (miR)-181a in serum of patients with Alzheimer′s disease (AD) and their diagnostic value.
Methods A total of 140 AD patients diagnosed and treated in our hospital were selected as the research subjects (AD group), and they were divided into the mild group (n=52),the moderate group (n=49), and the severe group (n=39) according to the clinical dementia scale, and 50 healthy people who underwent physical examination during the same period were used as the control group. The relative expression of serum miR-181a was detected by real-time fluorescence quantitative PCR (RT-qPCR), and the levels of serum TNF-α and M-CSF were detected by enzyme-linked immunosorbent assay (ELISA). The correlation of serum TNF-α, M-CSF and miR-181a was analyzed by Pearson correlation. The receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of serum TNF-α, M-CSF and miR-181a expression in AD.
Results The expression levels of serum TNF-α, M-CSF and miR-181a in AD group were significantly higher than those in control group (t=49.291, 30.485, 28.313, all P＜0.05). There were statistically significant differences in the expressions of serum TNF-α, M-CSF and miR-181a among the mild, moderate and severe groups (all P＜0.05). Serum TNF-α in AD patients was significantly and positively correlated with the expressions of M-CSF and miR-181a (r=0.524, 0.610, both P＜0.05), and M-CSF was significantly and positively correlated with the expression of miR-181a (r=0.498, P＜0.05). Serum TNF-α＞27.18 ng/L, serum M-CSF＞635.81 ng/L and serum miR-181a＞1.83 were the risk factors of AD. The areas under the ROC curve (AUC) of serum TNF-α, M-CSF,miR-181a and joint testing were 0.741 (95%CI: 0.684-0.789), 0.693 (95%CI: 0.630-0.757), 0.727 (95%CI: 0.682-0.774) respectively, and 0.862 (95%CI: 0.818-0.899), and the AUC of combined detection in diagnosing AD was greater than that of TNF-α, M-CSF and miR-181a alone in diagnosis (Z=3.357, 5.894, 4.911, all P＜0.05).
Conclusion The expression of serum TNF-α, M-CSF and miR-181a in AD patients is increased, and the increased levels of the three are independent risk factors for AD, and the combined detection of the three has high value in the diagnosis of AD.

Key words: Alzheimer′s disease, tumor necrosis factor-α, macrophage colony-stimulating factor

付丹, 梁洪波, 孙东鹏, 李斌. 老年阿尔茨海默病患者血清中TNF-α、M-CSF及miR-181a的表达水平及临床应用价值[J]. 河北医科大学学报, 2023, 44(9): 1006-1010,1026.

FU Dan, LIANG Hong-bo, SUN Dong-peng, LI Bin. Expression levels and clinical application value of TNF-α, M-CSF and miR-181a in serum of elderly AD patients[J]. Journal of Hebei Medical University, 2023, 44(9): 1006-1010,1026.

[1]	尹国明1（综述）, 李强1, 孙会艳2（审校）. 表没食子儿茶素没食子酸酯在阿尔茨海默病中神经保护作用机制研究进展 [J]. 河北医科大学学报, 2024, 45(3): 361-367.
[2]	赵圆, 杨琳, 孙超然, 杨婷, 彭文涛, 刘春艳. 下颌前移矫治器对OSAHS腭咽组织和血浆中炎症因子的影响 [J]. 河北医科大学学报, 2023, 44(8): 883-887,940.
[3]	范素芳, 吕侯强. 血糖、炎症指标与COPD气流受限、治疗预后关联性及临床意义 [J]. 河北医科大学学报, 2023, 44(12): 1436-1441.
[4]	黄锐升, 林丽敏, 金泰, 李虹霓, 黄奕霞, 苏丹 . 干眼症患者泪液IL-1β、TNF-α、TSLP水平与BUT值、FL评分的相关性分析[J]. 河北医科大学学报, 2021, 42(4): 425-428.
[5]	孙志成, 王彤, 马金霖, 张娜, 潘丽君, 董亚军. 虚拟现实情景互动训练在养老院阿尔茨海默病患者康复治疗中的应用[J]. 河北医科大学学报, 2021, 42(2): 222-227.
[6]	张微, 洪波, 柳安, 袁梦雅, 王静华, 王涛. 遗忘型轻度认知功能损害血浆中竞争内源性RNA网络构建及分析[J]. 河北医科大学学报, 2021, 42(10): 1128-1132,1148.
[7]	张〓冲1，郭〓威2（综述），廖小明1*（审校）. 维生素E与阿尔茨海默病关系的研究进展#br#[J]. 河北医科大学学报, 2018, 38(4): 481-485.
[8]	刘〓莎1，宫〓蕊2，吴〓明1，刘俊骞3，魏树林2，刘增娟1*. hUCMSCs通过抑制星形胶质细胞活化引起的炎症因子表达改善Aβ损伤大鼠学习记忆能力[J]. 河北医科大学学报, 2018, 38(3): 258-262.
[9]	李鹏涛1，高志红1，段晓亮2，张晓丽3，张〓辉3*. 黄芪甲苷Ⅳ诱导SAMR1小鼠骨髓间充质干细胞分化为神经细胞的实验研究[J]. 河北医科大学学报, 2018, 38(11): 1338-1343.
[10]	方〓婷1(综述)，肖〓军1，2(审校). 听理解障碍在识别早期阿尔茨海默病及轻度认知障碍中的价值[J]. 河北医科大学学报, 2017, 37(9): 1096-1099.
[11]	刘〓娜1，王金华1，王华龙1，邱会卿1，王彦永1,2*，王铭维1,2. 红景天对老年痴呆大鼠认知功能的治疗作用[J]. 河北医科大学学报, 2017, 38(2): 133-137.
[12]	温〓涵1（综述），温立斌2*（审校）. 现代电子技术在阿尔茨海默病检测中的应用[J]. 河北医科大学学报, 2017, 37(1): 102-107.
[13]	贾阳娟1，韩〓凝1，王美蓉1，贾艳丽2，吕佩源2，王建华1，2*. 艾地苯醌与多奈哌齐治疗轻中度阿尔茨海默病效果比较[J]. 河北医科大学学报, 2016, 37(11): 1317-1320.
[14]	李琦军;常军英;吴永波;邢兆国;周红艳;贾东昭. 神经肽Y对小神经胶质细胞活化状态和生成TNF-α的影响及其作用机制[J]. , 2015, 36(4): 463-463.
[15]	张爱丽;顾振芳;杨晶;韩晓雯. 慢性阻塞性肺疾病患者血清脂联素与营养状况、炎症因子的相关性[J]. , 2014, 35(1): 59-59.

老年阿尔茨海默病患者血清中TNF-α、M-CSF及miR-181a的表达水平及临床应用价值

Expression levels and clinical application value of TNF-α, M-CSF and miR-181a in serum of elderly AD patients

HTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价