月经血间充质干细胞和人脐带间充质干细胞的生物学特性比较

doi:10.3969/j.issn.1007-3205.2023.12.002

河北医科大学学报 ›› 2023, Vol. 44 ›› Issue (12): 1370-1378.doi: 10.3969/j.issn.1007-3205.2023.12.002

月经血间充质干细胞和人脐带间充质干细胞的生物学特性比较

1.河北医科大学基础医学院组织胚胎学教研室，河北石家庄 050017；2.河北省生殖健康医院干细胞实验室，河北石家庄 050051

出版日期:2024-01-02 发布日期:2024-01-02
作者简介:孙聪（1997-），女，河北宁晋人，河北医科大学基础医学院医学硕士研究生，从事干细胞基础研究。
基金资助:
河北省自然科学基金项目（H2020206243）

Comparison of biological characteristics between menstrual blood mesenchymal stem cells and human umbilical cord mesenchymal stem cells

1.Department of Histology and Embryology, the School of Basic Hebei Medical University, Shijiazhuang
050017, China; 2.Stem Cell Laboratory, Hebei Reproductive Health Hospital,
Shijiazhuang 050051, China

Online:2024-01-02 Published:2024-01-02

摘要/Abstract

摘要： 目的比较月经血间充质干细胞（menstrual blood mesenchymal stem cells，MenSCs）和人脐带间充质干细胞（human umbilical cord mesenchymal stem cells，hUCMSCs）的生物学特性。
方法从月经血和脐带中分离间充质干细胞（mesenchymal stem cells，MSCs），通过流式细胞术、免疫细胞化学、CCK8法比较两种干细胞表面标记物、细胞增殖情况；实验分组分为对照组、MenSCs-CM组、hUCMSCs-CM组，采用CCK8法、划痕、血管形成实验检测两种干细胞对人脐带静脉内皮细胞（human umbilical vein endothelial cells，HUVECs）的增殖、迁移以及血管形成能力的影响。
结果两种干细胞都贴壁生长，呈梭形；均高表达CD90、CD44、CD73、CD105，低表达CD14、CD45、CD34，不表达HLA-DR；MenSCs和hUCMSCs均表达SOX2及VEGFA，但MenSCs还高表达OCT4；MenSCs-CM组和hUCMSCs-CM组对HUVECs增殖、迁移率和血管形成的作用均高于对照组（P＜0.05）；MenSCs-CM组对HUVECs增殖和血管形成的作用明显高于hUCMSCs-CM组（P＜0.05）。
结论两种细胞均具有间充质干细胞的特性，但MenSCs还具有胚胎干细胞的特性；MenSCs和hUCMSCs均能促进HUVECs增殖、迁移以及血管形成，但MenSCs作用强于hUCMSCs。

关键词: 间充质干细胞, 内皮细胞, 增殖, 血管形成

Abstract: Objective To compare the biological characteristics of menstrual blood mesenchymal stem cells (MenSCs) with human umbilical cord mesenchymal stem cells (hUCMSCs).
Methods Mesenchymal stem cells (MSCs) were isolated from menstrual blood and umbilical cord. The surface markers and cell proliferation of two kinds of stem cells were compared by flow cytometry, immunocytochemistry and CCK8 assay. Experimental groups were divided into control group, MenSCs-CM group, and hUCMSCs-CM group. The effects of two kinds of stem cells on the proliferation, migration and angiogenesis on human umbilical vein endothelial cells (HUVECs) were detected by CCK8 assay, scratch assay and angiogenesis assay.
Results Both kinds of stem cells adhered to the wall and were fusiform. There was high expression of CD90, CD44, CD73, CD105, low expression of CD14, CD45, CD34, and no expression of HLA-DR. Both MenSCs and hUCMSCs expressed SOX2 and VEGFA, but MenSCs had highly expressed OCT4. The results showed that proliferation, migration and angiogenesis of HUVECs in both the MenSCs-CM group and hUCMSCs-CM group were higher than those in the control group (P＜0.05). The MenSCs-CM group was superior to the hUCMSCs-CM group with respect to proliferation and angiogenesis of HUVECs (P＜0.05).
Conclusion Both kinds of cells have the properties of mesenchymal stem cells, but MenSCs also have the properties of embryonic stem cells. Both MenSCs and hUCMSCs can promote the proliferation, migration and angiogenesis of HUVECs, but the effect of MenSCs is better than hUCMSCs.

Key words: mesenchymal stem cells, endothelial cells, proliferation, angiogenesis

孙聪, 薛国钰, 周贵媛, 李京泽, 陈炜, 邵素霞. 月经血间充质干细胞和人脐带间充质干细胞的生物学特性比较 [J]. 河北医科大学学报, 2023, 44(12): 1370-1378.

SUN Cong, XUE Guo-yu, ZHOU Gui-yuan, LI Jing-ze, CHEN Wei, SHAO Su-xia. Comparison of biological characteristics between menstrual blood mesenchymal stem cells and human umbilical cord mesenchymal stem cells [J]. Journal of Hebei Medical University, 2023, 44(12): 1370-1378.

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月经血间充质干细胞和人脐带间充质干细胞的生物学特性比较

Comparison of biological characteristics between menstrual blood mesenchymal stem cells and human umbilical cord mesenchymal stem cells

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