河北医科大学学报

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膳食脂肪含量的改变对糖尿病大鼠胃促生长素水平的影响

  

  1. 河北省沧州市人民医院内分泌科,河北 沧州 061000
  • 出版日期:2016-12-25 发布日期:2016-12-29
  • 作者简介:陈云霞(1969-),女,河北沧州人,河北省沧州市人民医院主任医师,医学硕士,从事内分泌疾病诊治研究。

Effect of changes in dietary fat content on the ghrelin levels in diabetic rats

  1. Department of Endocrinology, the People′s Hospital of Cangzhou City, Hebei Province, Cangzhou 061000, China
  • Online:2016-12-25 Published:2016-12-29

摘要: [摘要] 目的 观察糖尿病大鼠膳食脂肪含量变化对体质量、不同部位脂肪含量及胃促生长素的影响。方法
将 60 只健康雄性 SD 大鼠,分为正常对照组 15 只,余 45 只大鼠给予高脂饲料喂养 8 周,联合小剂量链脲佐菌素腹
腔注射建立糖尿病模型,造模成功 36 只。采用随机数字表法将造模成功的 36 只大鼠分为普通饮食糖尿病组和高
脂饮食糖尿病组各 18 只。正常对照组和普通饲料糖尿病组给予普通饲料,高脂饮食糖尿病组给予高脂饲料,喂养
6 周后处死大鼠,收集血液和脂肪组织,采用酶联免疫吸附测定方法检测血浆胃促生长素水平。结果 普通饮食糖
尿病组和高脂饮食糖尿病组大鼠体质量、附睾的脂肪、肾周脂肪、肠系膜脂肪均高于正常对照组,胃促生长素均低
于正常对照组。结论 膳食脂肪含量的变化对血浆胃促生长素水平有调节作用,能影响体质量及能量摄入

关键词: 糖尿病, 膳食脂肪类, 胃促生长素, 大鼠

Abstract: [
Abstract ] Objective Tostudytheregulationofdietaryfatindiabeticratsontheweight ,
differentpartsofthefatandghrelin.Methods SixtymaleSDratsweredividedintotwogroups
randomly : 15normalcontrolratsand45testrats.Thetestratswerefedwithhighfatdietfor
eightweeksandasingleintraperitonealinjectionofstreptozotocin ( STZ , 30mg /
kg
) toestablish
ananimalmodelofdiabetes.36diabeticratmodelwassuccessfullyestablished.Thediabeticrats
wererandomlydividedintofollowinggroups : 18generaldietdiabeticratsand18high-fatdiet
diabeticrats.Thenormalcontrolratsandgeneraldietdiabeticratswerefedwithgeneraldiet.The
high-fatdietdiabeticratswerefedwithhigh-fatdiet.Aftersixweeks , allratswerekilled.Blood
andadiposetissuesampleswerecollectedinallrats.Serum ghrelinwasdetectedbyenzyme-
linkedimmunoassay.Results Thegeneraldietdiabeticratsandhigh-fatdietdiabeticratswere
significantlyhigherin weight , epididymalfat , epinephrinefatand mesentericfatthannormal
controlrats , andweresignificantlylowerinplasmaghrelinconcentrationthannormalcontrol
rats.Conclusion Changesindietaryfatcontentplayaregulatoryroleonlevelsofghrelinin
plasmathataffectweightandenergyintake.

Key words: diabetes mellitus, diettary fats; ghrelin, rats