鲑鱼降钙素及甲状旁腺素（1-34）对卵巢切除大鼠椎体骨密度及骨组织形态计量学影响的比较

doi:10.3969/j.issn.10073205.2017.12.008

河北医科大学学报

鲑鱼降钙素及甲状旁腺素（1-34）对卵巢切除大鼠椎体骨密度及骨组织形态计量学影响的比较

1.河北医科大学第三医院创伤急救中心，河北石家庄 050051;2.华北理工大学医学实验中心，河北唐山 063000;
3.华北理工大学附属医院骨科，河北唐山 063000

出版日期:2017-12-25 发布日期:2017-12-19
作者简介:骆阳（1982-），男，河北石家庄人，河北医科大学第三医院主治医师，医学博士，从事骨外科疾病诊治研究。
基金资助:
河北省医学科学研究重点课题（20170137）

Comparison of the effects of salmon calcitonin and parathyroid hormone(134) on bone mineral density and bone histomorphometry of vertebra in ovariectomized rats#br#

1.Department of Orthopedic Surgery, the Third Hospital of Hebei Medical University, Shijiazhuang
050051, China; 2.Medical Research Center, the North China University of Science and Technology,
Tangshan 063000, China; 3.Department of Orthopaedic Surgery, the Affiliated Hospital of
North China University of Science and Technology, Tangshan 063000, China

Online:2017-12-25 Published:2017-12-19

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗比较鲑鱼降钙素（salmon calcitonin,CT ）及甲状旁腺素（ parathyroid hormone,PTH）(134)对卵巢切除大鼠腰椎骨密度(bone mineral density,BMD)及骨组织形态计量学的影响。
〖HTH〗方法〖HTSS〗〖KG*2〗40只3月龄雌性SpragueDawley（SD）大鼠，随机分为4组，每组10只：假手术（Sham）组；双侧卵巢切除（OVX）组；双侧卵巢切除+甲状旁腺素（134）（OVX+PTH）组；双侧7卵巢切除+鲑鱼降钙素（OVX+CT）组。OVX+CT组及OVX+PTH组于卵巢切除术后3个月分别给予皮下注射CT或PTH(134)。连续给药3个月后处死所有大鼠并收集标本。取L4椎体进行BMD检测。L5椎体进行Gemisa染色及骨组织形态计量学检测。
〖HTH〗结果〖HTSS〗〖KG*2〗卵巢切除术后6个月，OVX组BMD显著低于Sham组（P＜005），而OVX+CT组及OVX+PTH组椎体BMD显著高于OVX组（P＜005），且OVX+PTH组BMD显著高于OVX+CT组（P＜005）。OVX组BV/TV低于Sham组，OVX+CT组高于OVX组，OVX+PTH组BV/TV高于Sham组、OVX组、OVX+CT组，差异均有统计学意义（P＜005）。OVX组、OVX+CT组Tb.Th低于Sham组，OVX+PTH组高于Sham组、OVX组、OVX+CT组，差异均有统计学意义（P＜005）。OVX组Tb.Sp高于Sham组，OVX+CT组和OVX+PTH组低于OVX组，差异均有统计学意义（P＜005）。各组大鼠Tb.N差异无统计学意义（P＞005）。
〖HTH〗结论〖HTSS〗〖KG*2〗CT及PTH（134）可显著增加卵巢切除大鼠腰椎BMD并抑制骨重建的高转换状态，且PTH（134）作用要优于CT。

关键词: 骨质疏松, 骨密度, 甲状旁腺, 降钙素

Abstract: ［Abstract］ Objective〖HTSS〗〓To compare the effects of salmon calcitonin(CT) and parathyroid hormone(PTH)(134) on bone mineral density and bone histomorphometry of vertebra in ovariectomized rats.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Forty female SpragueDawley rats aged 3 months were randomly divided into four groups(with 10 rats each). All rats but those in the 〖JP2〗Sham group received ovariectomy and treated by vehicle(OVX), salmon calcitonin(OVX+CT) and PTH(134)(OVX+〖JP〗PTH). After animals were sacrificed at 6 months postOVX, the L4-5 spinal segments were harvested. Bone mineral density(BMD) and histomorphometry analysis were performed to evaluate the bone mass and microstructural changes in the lumbar vertebral bodies．Histological analysis with Gemisa staining was used to identify the characteristics of the degenerative discs.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Six months after OVX, the BMD values of L4 vertebral bodies 〖JP2〗in the OVX group were significantly decreased, compared with the Sham group(P＜005)．The BMD values in the OVX+〖JP〗CT group and OVX+PTH group were significantly increased，compared with the OVX group(P＜005), and the BMD values in the OVX+PTH group were significantly increased than that in the OVX+CT group(P＜005). The BV/TV values of L5 vertebral bodies in the OVX group were significantly decreased, compared with the Sham group, the BV/TV values in the OVX+CT group were significantly increased than that in the OVX group, the BV/TV values in the OVX+PTH group were significantly increased, compared with the Sham, OVX and OVX+CT group(P＜005). The Tb.Th values in the OVX group and OVX+CT group were significantly decreased, compared with the Sham group, the Tb.Th values in the OVX+PTH group were significantly increased than that in the Sham group, OVX group and OVX+CT group(P＜005). The values of Tb.Sp in the OVX group were higher than that in the Sham group, OVX+CT group and OVX+PTH group(P＜005). There are no significantly changes of Tb.N values in each groups(P＞005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Both salmon calcitonin and parathyroid hormone(134) can improve the bone mass and suppress the high bone turnover in OVX rats. But the effect of parathyroid hormone(134) to suppress the bone resorption and improve the bone mass is better than that of salmon calcitonin.

Key words: osteoporosis, bone density, parathyroid hormone, calcitonin

骆〓阳1，齐〓璨1，罗鹏远1，梁少博1，田发明2，张〓柳3. 鲑鱼降钙素及甲状旁腺素（1-34）对卵巢切除大鼠椎体骨密度及骨组织形态计量学影响的比较[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2017.12.008.

LUO Yang1， QI Can1， LUO Pengyuan1, LIANG Shaobo1, TIAN Faming2， ZHANG Liu3. Comparison of the effects of salmon calcitonin and parathyroid hormone(134) on bone mineral density and bone histomorphometry of vertebra in ovariectomized rats#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2017.12.008.

[1]	张欣. 血液CC16、PCT、FKN水平与慢性阻塞性肺疾病患者衰弱的临床研究 [J]. 河北医科大学学报, 2023, 44(9): 1027-1031.
[2]	徐琦量, 陈晓明, 戴强, 施文娟, 周剑, 蒋逸. 血乳酸、降钙素原清除率对烧伤休克患者预后的预测价值 [J]. 河北医科大学学报, 2023, 44(8): 941-944,951.
[3]	李素华, 苗毅, 张爱民. 血清PCT、NT-proBNP、mtDNA水平与CPB心脏术后早期并发症的关系及其预测价值研究 [J]. 河北医科大学学报, 2023, 44(5): 536-540,595.
[4]	张林, 陈晓钢, 季沐梵, 丁静, 刘青柏. 术前椎体压缩程度联合骨密度对胸腰椎骨折椎弓根螺钉内固定术后椎体高度再丢失的预测 [J]. 河北医科大学学报, 2023, 44(3): 274-278.
[5]	朱明明, 李宗虎, 郑宪玲, 王颖霞, 张树杰, 张瑞清. 不同骨密度水平的糖尿病患者外周血Omentin-1水平、脂代谢、氧化应激的表达及意义 [J]. 河北医科大学学报, 2023, 44(11): 1267-1273.
[6]	房启文, 李海新, 许娜娜, 马思思, 王蕾, 董志玲. PCT、hs-CRP及sPLA2-ⅡA检测在脓毒症临床评价中的应用价值 [J]. 河北医科大学学报, 2023, 44(10): 1157-1161，1184.
[7]	宋昕, 孙莹, 张琦, 耿涛. 基于RANKL/RANK/OPG通路研究绝经后老年骨质疏松患者骨代谢与肠道菌群失调的关系[J]. 河北医科大学学报, 2022, 43(3): 302-306.
[8]	张林, 雷小保, 熊劲芝, 黄强, 李娜. 神经外科重症患者CRE感染发生危险因素及NLR、PCT、MCP-1对感染的鉴别诊断价值[J]. 河北医科大学学报, 2022, 43(3): 339-344.
[9]	张赟, 陈娟, 黄尧米. 血浆Th1/Th2细胞、ET、PTA、PCT与脓毒症患儿病情严重程度及转归的关系分析 [J]. 河北医科大学学报, 2022, 43(12): 1449-1453.
[10]	于冬, 温莉, 张涛, 张书晨, 崔为英. 超声综合评分与慢性肾脏病-矿物质和骨代谢紊乱患者甲状旁腺功能的相关性研究[J]. 河北医科大学学报, 2022, 43(1): 91-95.
[11]	杨朝旭, 邢栋, 张隆, 郑淑慧, 李文毅, 朱仕文. 老年髋部骨折术后对侧髋部再骨折的发生率及危险因素分析[J]. 河北医科大学学报, 2021, 42(1): 30-33.
[12]	李云1，崔丽萍1，董文荣1,郑飞2. 良性阵发性位置性眩晕与骨密度的研究#br#[J]. 河北医科大学学报, 2020, 41(9): 1070-1074.
[13]	姚柯炜，张流畅. 股骨外侧壁厚度对于股骨近端骨折术后外侧壁骨折的预测价值[J]. 河北医科大学学报, 2020, 41(6): 738-741.
[14]	张鹏1，张迪1，李向科2，韩永台2，申勇1. 降钙素原水平与髋关节结核病变严重程度及预后的相关性研究[J]. 河北医科大学学报, 2020, 41(4): 405-408.
[15]	崔照领1，李扬2，朱芳3，莫中福1，曹珍珍1，郭春霞4. 妊娠期高血压疾病对新生儿凝血功能、NSE及PCT水平的影响[J]. 河北医科大学学报, 2020, 41(3): 306-310.

鲑鱼降钙素及甲状旁腺素（1-34）对卵巢切除大鼠椎体骨密度及骨组织形态计量学影响的比较

Comparison of the effects of salmon calcitonin and parathyroid hormone(134) on bone mineral density and bone histomorphometry of vertebra in ovariectomized rats#br#

HTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价