2种实验性增生性玻璃体视网膜病变造模方法比较研究

doi:10.3969/j.issn.10073205.2018.10.020

河北医科大学学报

2种实验性增生性玻璃体视网膜病变造模方法比较研究

河北医科大学第二医院眼科，河北石家庄 050000

出版日期:2018-10-25 发布日期:2018-09-27
作者简介:刘丽娅（1981-），女，天津武清人，河北医科大学第二医院主治医师，医学博士，从事眼底疾病诊治研究。
基金资助:
河北省医学科学研究重点课题（20170074）

Comparison of two methods for modeling proliferative vitreoretinopathy

Department of Ophthalmology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China

Online:2018-10-25 Published:2018-09-27

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗采用2种方法进行兔眼内增生性玻璃体视网膜病变（proliferative vitreoretinopathy,PVR）模型的建立，对比2种方法的成模时间和效果。
〖HTH〗方法〖HTSS〗〖KG*2〗两实验组分别给予玻璃体腔注射视网膜色素上皮（retinal pigment epithelium，RPE）细胞与白细胞介素1β（interleukin1β，IL1β）的混合液或单纯RPE细胞进行造模，对照组给予玻璃体腔注射生理盐水，术后进行玻璃体、眼底、B超等检查，对玻璃体混浊程度和PVR程度进行分级，并计算视网膜脱离发生率。
〖HTH〗结果〖HTSS〗〖KG*2〗术后7 d 2组PVR发生率均为100%，但注射RPE细胞联合IL1β组较单纯注射RPE细胞组PVR发生更早、程度更重、视网膜脱离发生率更高。
〖HTH〗结论〖HTSS〗〖KG*2〗通过玻璃体腔注射RPE细胞联合IL1β建立兔眼PVR模型的方法较单纯注射RPE细胞效果更佳，更符合PVR的病理过程。

关键词: 玻璃体视网膜病, 增生性, 视网膜色素上皮, 视网膜脱离

Abstract: ［Abstract］ Objective〖HTSS〗〓To compare the time and effect of the 2 methods by using 2 methods to establish proliferative vitreoretinopathy(PVR) model in rabbit eye.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The experimental group was given intravitreal injection of a mixture of retinal pigment epithelium (RPE) cells and interleukin1(IL1) beta or simple RPE cells respectively. The control group was given intravitreal injection of normal saline, and vitreous, fundus and B ultrasonic examination were performed after operation. The degree of vitreous opacity and the degree of PVR were graded, and the incidence of retinal detachment was calculated.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓After 7 days of vitreous injection, occurrences of PVR were 100% in both of the two groups. PVR happened more earlier and faster in Group Ⅰ than Group Ⅱ. Group Ⅰ also had the higher incidence of retinal detachment.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Injective vitreous of RPE cells combined with IL1β in rabbit eyes is the best method to establish of PVR model, and is more in line with the pathological process of PVR.

Key words: vitreoretinopathy, proliferative, retinal pigment epithelium, retinal detachment

刘丽娅，马景学*，安建斌，马月磊. 2种实验性增生性玻璃体视网膜病变造模方法比较研究[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2018.10.020.

LIU Liya, MA Jingxue*, AN Jianbin, MA Yuelei. Comparison of two methods for modeling proliferative vitreoretinopathy[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2018.10.020.

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2种实验性增生性玻璃体视网膜病变造模方法比较研究

Comparison of two methods for modeling proliferative vitreoretinopathy

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