河北医科大学学报 ›› 2022, Vol. 43 ›› Issue (1): 7-11.doi: 10.3969/j.issn.1007-3205.2022.01.002

• • 上一篇    下一篇

原花青素B2对PM2.5急性暴露致大鼠心脏毒性的保护作用

  

  1. 河北医科大学公共卫生学院，河北省环境与人群健康重点实验室，河北 石家庄 050017
  • 出版日期:2022-01-25 发布日期:2022-01-24
  • 作者简介:任敬一（1997-），男，河北承德人，河北医科大学公共卫生学院医学硕士研究生，从事营养与食品卫生学研究。
  • 基金资助:
    国家自然科学基金（81874264）

The protective effect of procyanidin B2 on cardiotoxicity induced by acute expose to PM2.5 in rats 

  1. School of Public Health, Hebei Medical University， Key Laboratory of Environment and Human Health of Hebei Province, Shijiazhuang 050017, China
  • Online:2022-01-25 Published:2022-01-24

HTML

PDF (PC)

144

摘要： 目的  探讨原花青素B2（procyanidin B2,PCB2）对PM2.5急性暴露致大鼠心脏毒性的保护作用。
方法  24只雄性健康成年SD大鼠按照随机数字表法分为三组：生理盐水对照组（持续三蒸水灌胃21 d，在第8天、第10天、第12天气管滴注生理盐水）、PM2.5组（持续三蒸水灌胃21 d，在第8天、第10天、第12天气管滴注PM2.5悬浊液，10 mg/kg）、PCB2组（持续PCB2灌胃21 d，40 mg/kg，在第8天、第10天、第12天气管滴注PM2.5悬浊液）。末次灌胃24 h后，处死大鼠，取心脏，制作组织切片，并检测心脏中总超氧化物歧化酶(superoxide dismutase,SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）、丙二醛（malondialdehyde,MDA）、白细胞介素1β（interleukin-1β,IL-1β）、白细胞介素6（interleukin-6,IL-6）和白细胞介素18（interleukin-18,IL-18）的水平。
结果  与生理盐水对照组比较，PM2.5组大鼠心脏组织匀浆中的IL-1β、IL-6、IL-18和MDA含量均明显升高，GSH-Px、SOD含量明显下降，差异均有统计学意义（P＜0.05），与PM2.5组相比，PCB2组大鼠心脏组织匀浆的IL-1β和IL-18含量均明显降低，SOD和GSH-Px含量升高，差异均有统计学意义（P＜0.05）。
结论  急性气管滴注PM2.5可明显引起大鼠心脏组织炎症和氧化应激指标改变，PCB2对PM2.5引起的心脏损伤具有保护作用。


关键词: 原花青素, 心脏毒性, 颗粒物

Abstract: Objective  To explore the protective effect of procyanidin B2(PCB2) on  cardiotoxicity induced by acute exposure to PM2.5 in rats. 
Methods  A total of 24 healthy adult male SD rats were divided into three groups: the normal saline control group(the rats were given distilled water by gavage three time a day for 21 consecutive days and were exposed to 0.9% saline by intratracheal instillation on the 8th, 10th, and 12th days), PM2.5 group(the rats were given distilled water by gavage three times a day for 21 consecutive days and were exposed to PM2.5 suspension by intratracheal instillation on the 8th, 10th, and 12th days), and PCB2 group(the rats were pretreated with PCB2 by gavage for 21 consecutive days and were exposed to PM2.5 suspension by intratracheal instillation on the 8th, 10th, and 12th days) according to random number table method. At 24 h after the last gavage, the rats were sacrificed and cardiac tissues were collected for prepartion of tissue section. The expressions of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), malondialdehyde(MDA), interleukin-1β(IL-1β), interleukin-6(IL-6) and interleukin-18(IL-18) were detected. 
Results  Compared with the normal saline control group, the contents of IL-1β, IL-6, IL-18 and MDA in the heart tissue homogenate of PM2.5 group were significantly increased, while the contents of GSH-Px and SOD in PM2.5 group were decreased significantly, suggesting significant differences(P＜0.05). Compared with the PM2.5 group, the contents of IL-1β and IL-18 in the heart tissue homogenate of PCB2 group were significantly reduced, and the contents of SOD and GSH-Px in PCB2 group were increased significantly, suggesting significant differences(P＜0.05). 
Conclusion  PM2.5 by acute intratracheal instillation can obviously cause inflammatory damage and oxidative stress changes in rats. PCB2 has a protective effect against heart damage caused by PM2.5.


Key words: procyanidin, cardiotoxicity, particulate matter

版权所有 © 《河北医科大学学报》编辑部
本系统由北京玛格泰克科技发展有限公司设计开发