儿茶酚胺在束缚应激大鼠肺损伤中的作用#br#

doi:10.3969/j.issn.10073205.2017.05.002

河北医科大学学报

儿茶酚胺在束缚应激大鼠肺损伤中的作用#br#

1.华北理工大学附属医院呼吸内科，河北唐山 063000；2.河北医科大学法医系，河北省法医学重点实验室，河北
石家庄 050017；3.河北省胸科医院骨科，河北石家庄 050042；4.河北医科大学第二医院感染科，河北石家庄 050000

出版日期:2017-05-25 发布日期:2017-05-23
作者简介:李莉（1989-），女，河北石家庄人，华北理工大学附属医院医学硕士研究生，从事呼吸内科疾病诊治研究。
基金资助:
国家自然科学基金资助课题（81202382）

Effects of catecholamines on lung injury induced by restraint stress in rats#br#

1.Department of Respiratory Medicine, North China University of Science and Technology, Tangshan
063000, China; 2.Department of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory
of Forensic Medicine, Shijiazhuang 050017, China; 3.Department of Orthopedics, Hebei Chest
Hospital, Shijiazhuang 050042, China; 4.Department of Infectious Disease, the Second
Hospital of Hebei Medical University, Shijiazhuang 050000, China

Online:2017-05-25 Published:2017-05-23

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗研究儿茶酚胺（catecholamine，CA）在束缚应激大鼠肺损伤中的作用。
〖HTH〗方法〖HTSS〗〖KG*2〗选取成年SD雄性大鼠15只，随机分为对照组、束缚应激组、束缚+酪氨酸羟化酶抑制剂（alphamethylptyrosine，AMPT）组，每组5只。采用酶联免疫吸附测定法（enzymelinked immunosorbent assay，ELISA）检测血浆中去甲肾上腺素（norepinephrine，NE）、肾上腺素（epinephrine，E）水平，苏木素-伊红（hematoxylineosin，HE）染色法观察肺组织病理形态学变化，ELISA法检测肺组织中白细胞介素1β（interleukin1 beta，IL1β）及白细胞介素6（interleukin6，IL6）的浓度，二辛可酸法检测支气管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）中蛋白的含量。
〖HTH〗结果〖HTSS〗〖KG*2〗束缚应激组、束缚+AMPT组血浆中NE、E水平显著高于对照组，束缚+AMPT组血浆中NE、E水平显著低于束缚应激组（P＜005）；束缚应激组、束缚+AMPT组肺组织中IL1β、IL6浓度和BALF中蛋白含量均显著高于对照组，束缚+AMPT组肺组织IL1β、IL6浓度和BALF中蛋白含量显著低于束缚应激组，差异有统计学意义（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗束缚应激可致肺损伤，CA可能参与这一损伤过程，AMPT可明显减轻肺损伤。

关键词: 肺损伤, 儿茶酚胺, 束缚应激

Abstract: Abstract］ Objective〖HTSS〗〓To study the effect of catecholamine(CA) on lung injury in rats with restraint stress.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fifteen adult male SD rats were randomly divided into control group, restraint stress group and restraint+alphamethylptyrosine(AMPT) group, 5 rats in each group. Both plasma norepinephrine(NE), epinephrine(E) and interleukin1 beta(IL1β), interleukin6(IL6) concentration were assayed by enzymelinked immunosorbent assay(ELISA). Hematoxylineosin(HE) staining was used to observe the pathological changes of lung tissue. The protein levels in the bronchoalveolar lavage fluid(BALF) were detected by bicinchoninic acid method.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with the control group, the plasma NE and E levels in the restraint stress group, restraint+AMPT group were significantly higher than those in the control group. Compared with the restraint stress group, the levels of NE and E in plasma of the restraint+AMPT group were significantly lower than those in restraint stress group(P＜005). Compared with the control group, the levels of IL1β, IL6 in the lung tissue and BALF protein content of the restraint stress group and restraint+AMPT group were significantly increased. Compared with the restraint stress group, the levels of IL1β, IL6 and BALF in the lung tissue of the restraint+AMPT group were significantly decreased, the difference was statistically significant(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Catecholamine may be involved in the lung injury induced by restraint stress. AMPT can significantly reduce the lung injury induced by restraint stress.

Key words: lung injury, catecholamine, restraint stress

李〓莉1，张晓静2，刘〓威3，宋〓宁4，喻昌利1*. 儿茶酚胺在束缚应激大鼠肺损伤中的作用#br#[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2017.05.002.

LI Li1, ZHANG Xiaojing2, LIU Wei3, SONG Ning4, YU Changli1*. Effects of catecholamines on lung injury induced by restraint stress in rats#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2017.05.002.

[1]	刘健, 宋然, 宋朝国, 张健. 主动脉夹层合并急性肺损伤与血清CRP、MCP-1、MMP9的相关性[J]. 河北医科大学学报, 2020, 41(10): 1126-1129.
[2]	徐海博1，齐倩2，王伯丽1，阎锡新1*. 高浓度富氢生理盐水对脂多糖诱导大鼠急性肺损伤的作用[J]. 河北医科大学学报, 2019, 40(12): 1372-1376.
[3]	高〓鹏，段亚伟，陈〓华*，肖宇杨，刘东霞，韩玉平. 嗜铬细胞瘤危象救治相关病例分析[J]. 河北医科大学学报, 2018, 38(12): 1479-1483.
[4]	甄洁1，陈炜1*，朱曦2，边伟帅1，赵磊1，盛博1. 甲泼尼龙对盐酸诱导急性肺损伤大鼠的保护作用[J]. 河北医科大学学报, 2016, 37(6): 647-651.
[5]	李新江，方艳伟，魏浩飞，孙海滨，王川，赵建辉. 细胞间黏附分子1高表达与大鼠SAP早期急性肺损伤的关系[J]. 河北医科大学学报, 2016, 37(4): 392-396.
[6]	李新江 1,方艳伟 1,魏浩飞 1,孙海滨 1,王川 1,张剑 2. 早期持续性高容量血液滤过对重症急性胰腺炎急性肺损伤患者的影响[J]. 河北医科大学学报, 2016, 37(2): 198-201.
[7]	丁立春;冯平勇. 肺损伤的CT表现[J]. , 2004, 25(z1): 59-59.
[8]	霍萌;李英. 百草枯中毒的胸部CT分析[J]. , 2004, 25(z1): 56-56.
[9]	吕秀芹;王俊祥;何学峰;刘俊江. 以发热为主要表现的嗜铬细胞瘤1例[J]. , 2001, 22(6): 346-346.
[10]	谷振勇;凌亦凌;刘伟娜;刘满英;朱铁年;黄善生. 黄芩甙对过氧亚硝基阴离子致肺损伤的保护作用[J]. , 1999, 20(3): 172-172.

儿茶酚胺在束缚应激大鼠肺损伤中的作用#br#

Effects of catecholamines on lung injury induced by restraint stress in rats#br#

HTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价