关键词: 血管内膜, 增生, 跨膜蛋白, 肌细胞, 平滑肌

Abstract: ［Abstract］〓Objective〖HTSS〗〓To investigate the correlation of transmembrane protein endothelial and smooth muscle cellderived neuropilinlike molecule(ESDN) expression and vascular hyperplasia.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The intimal hyperplasia model of the Esdn knockout mice and control wild type mice(WT) were prepared by the common carotid artery ligation. HE staining was used to detect the neointima fromation at different time points. Western blot was used to detect the expression of smooth muscle αactin(SM αactin), SM22α and osteopontin(OPN), the phenotype marker genes in vascular smooth muscle cells. Immunofluorescence was used to detect the SM αactin protein expression and distribution in aorta.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓At 7, 14 and 21 days after arterial ligation, the area of the intimal hyperplasia and the I/M ratio were significantly increased in the Esdn-/- arteries compared with WT control arteries. The expression of SM αactin and SM22α were decreased. However, the expression of OPN, the synthetic marker gene, was increased in the Esdn-/- arteries at 3, 7, 14 and 21 days after arterial ligation. Furthermore, the expression of SM αactin was enhanced in the media area of aorta in Esdn-/- arteries using immunofluorescence staining.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The expression of ESDN may be involved in vascular smooth muscle cell phenotypic switching and vascular remodeling.

Key words: tunica intima； hyperplasia； transmembrane proteins, myocytes, smooth muscle