Abstract: ［Abstract］〓Objective〖HTSS〗〓To explore the correlation of T lymphocytes with virus replication and viral load in children with hepatitis B.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fiftyeight children with chronic hepatitis B, who were positive for hepatitis B virusdesoxyribose nucleic acid(HBVDNA), were selected as the research subjects. Among them, 41 had positive human hepatitis B virus e antigen（HBeAg）, 17 had negative HBeAg, 24 had low viral load, 19 had moderate viral load, and 15 had high viral load. Moreover, 35 healthy children were served as the control group. FQPCR was used to detect HBVDNA. FCM was used to detect CD3+, CD4+, and CD8+. CD4+/CD8+ were calculated.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓CD3+, CD4+, CD4+/CD8+ in children with Chronic hepatitis B were lower than the control group, CD8+ was higher than the control group (P＜001). CD3+, CD4+, CD4+/CD8+ in load group were lower than that of low load group,  CD3+, CD4+, CD4+/CD8+in higher load group were lower than that of low load group and load group(P＜001), load group and high load of CD8+ was higher than that of low load group(P＜001). The CD3+, CD4+ and CD4+/CD8+ of HBeAg positive children were lower than those of HBeAg negative children, and CD8+ was higher than that of HBeAg negative children(P＜001).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Changes of T lymphocyte subsets in children with hepatitis B was not only associated with HBV infection, but also correlated with the replication of HBVDNA, which can further aggravate the cellular immune dysfunction.

Key words: hepatitis B, Tlymphocyte subsets, virus replication, viral load