Abstract: ［Abstract］ Objective〖HTSS〗To investigate the expression and significance of hypoxia inducible factor1 α(HIF1α) and stromal cell derived factor 1(SDF1) in differentiated thyroid carcinoma.
〖HTH〗〖WTHZ〗Methods〖HTSS〗According to the presence or absence of metastasis, the patients with differentiated thyroid carcinoma（DTC）were divided into three groups: carcinoma in situ group, metastatic cancer group and control group (paracancerous tissue). The expression of HIF1α and SDF1 in tissues was detected by qPCR. At the same time, SW579 cells were cultured in vitro, knocking out HIF1α to detect the transcription and protein expression of SDF1 and cell proliferation and invasion ability. The transcription of SDF1 and cell proliferation and invasion ability of SW579 cells were detected by exogenous addition of SDF1 100 ng/mL，and the proliferation and migration ability of SW579 cells was detected with replenishing SDF1.
〖HTH〗〖WTHZ〗Results〖HTSS〗The transcriptional expression levels of HIF1α and SDF1 in the excised tissues of DTC patients were significantly higher than those in the adjacent tissues, and the expression levels in the metastasis group were significantly higher than those in the in situ cancer group. The transcription level of SDF1 in HIF1αsiRNA group and HIF1αsiRNA+SDF1 group was lower than that in siNC group and siNC+SDF1 group(P＜0.05). At the same time, the level of SDF1 protein in the HIF1αsiRNA group was also lower than that in the SiNC group. The cell proliferation and cell invasion of siNC+SDF1 group were higher than those of siNC group. The cell proliferation and cell invasion of HIF1αsiRNA group were lower than siNC group and siNC+SDF1 group. The cell proliferation and cell invasion of HIF1αsiRNA+SDF1 group were lower than those of siNC+SDF1 group, but higher than HIF1αsiRNA group, the difference was statistically significant(P＜0.05).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗HIF1α and SDF1 are highly expressed in DTC tumors, and HIF1α plays an important role in the metastasis of DTC by regulating SDF1.

Key words: thyroid neoplasms, hypoxiainducible factor 1, alpha subunit, chemokine CXCL12