Observation of the expression of VEGF, flk1, bax and bcl2 in the retina of different types of DR rat model

doi:10.3969/j.issn.10073205.2016.11.013

Abstract

Abstract: [ Abstract ] Objective To observethevariation ofvascularendothelialgrowthfactor
( VEGF ), flk-1 , bax , bcl-2expressioninPDRratretinaeatablishedbysymphysialinjectionand
todefiniteitssuperiority.Methods Sprague-Dawleyratswere madeasdiabeticretinopathy
modelsbysolitaryinjection withstreptozotocinintraperitoneallyorsymphysialinjection with
streptozocinintraperitoneally and VEGFintravitreouslyrespectively.Usingfactorialdesign
empiricalmethod , weobservedthevariationofVEGF , flk-1 , bax , bcl-2expressioninretinasof
diabeticratsestablished by different methodsbyimmunohistochemistry and measuredthe
averageopticaldensityvalueofVEGF , flk-1 , bax , bcl-2inretinalganglioncelllayerandinner
plexiformlayerbyMIAS1998typeimageanalysissystem.Results VEGF , flk-1 , bax , bcl-2were
expressedineachlayerofretinainbothgroupsexceptthelayerofrodsandcones.Thefrontthree
targetswereexpressedstrongestintheganglionandnervefiberlayer , whilebcl-2wasexpressed
thestrongestininnersegmentoftherodsandconeslayer.ThenextstrongestlayerofVEGF expressionwasthe Müllercells whilethenextstrongestlayerofbcl-2expression wasthe
ganglionand nervefiberlayer.Alltargetsexpressioninsymphysialinjection groups were
strongerthanthoseinsolitaryinjectiongroups.Bcl-2expressioninganglionandnervefiberlayer
at8 weeksafter VEGFinjection wasstrongerthanthatat2 weeks.Thestrongestbcl-2
expression wasininnerplexiform layerat8 weeksinsolitaryinjection groups , whilein
symphysialinjectiongroupsitwasinganglionandnervefiberlayerat2 weeksafterVEGF
injection.Conclusion PDRrat modelestablishedbysymphysialinjectioncanrepresentthe
pathologicalchangesofhumanPDRandhasitsscientificresearchworth.

Key words: iabeticretinopathy , vascularendothelialgrowthfactors , rats

DONG Baixia， BAO Yongqin， WANG Zhendong， WANG Jiayao. Observation of the expression of VEGF, flk1, bax and bcl2 in the retina of different types of DR rat model[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2016.11.013.

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Observation of the expression of VEGF, flk1, bax and bcl2 in the retina of different types of DR rat model

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