Abstract: ［Abstract］〓Objective〖HTSS〗〓To investigate the effect of Survivin gene expression on human renal carcinoma cell 786O proliferation.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓RNA inteference was used to transfect human renal cancer cell line 786O and silence Survivin gene expression. Expression of Survivin mRNA and protein were detected by reverse transcriptionpolymerase chain reaction(RTPCR) and Western blot assay, respectively. Change of PCNA protein expression was measured meanwhile. The proliferation of 786O cells was evaluated by MTS assay.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared to control siRNA/786O and nonsiRNA/786O, Survivin siRNA/786O displayed reduced Survivin expression of mRNA and protein(P＜005). Expression level of PCNA protein was also downregulated significantly in Survivin siRNA/786O cells(P＜005). No significant differences of Survivin mRNA, Survivin protein and PCNA protein were found between control siRNA/786O and nonsiRNA/786O(P＞0.05).  Proliferation activity of all three groups of 786O cells showed increasing trend obviously. The level of siRNA/786O cells proliferation was significantly lower than other groups. There were statistically significant differences between groups, at different time point, also analysis of interaction between groups with time point revealed statistical significance(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓RNA interference of Survivin gene can downregulate expression of Survivin mRNA, Survivin protein and PCNA protein expression with decreasing the proliferation activity of cells. The present study revealed underlying influence of Survivin gene expression on malignant proliferation of renal cancer.

Key words: kidney neoplasms, RNA interference, cell proliferation