Abstract: ［Abstract］ Objective〖HTSS〗〓To investigate the expression and clinical significance of signal transducer and activator of transcription 3（STAT3 ） and Cyclin D1 in  cholangiocarcinoma tissues.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Immunohistochemical staining was used to detect the expression of STAT3 and Cyclin D1 in 70 cases of cholangiocarcinoma and 15 cases of nontumor bile duct epithelium.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The expression of STAT3 and Cyclin D1 in cholangiocarcinoma(61.4%, 42.9%) was significantly higher than that in nontumor bile duct epithelium(33.3%, 13.3%)(P＜005). STAT3 expression significantly correlated with clinical stage, lymph nodes metastasis and tumor grade. The positive rates of STAT3 was significantly higher in the stage Ⅲ+Ⅳ group , lymph nodes metastasis group and moderately or poorlydifferentiated group than those in stageⅠ+Ⅱ group, no lymph nodes metastasis group and welldifferentiated group(P＜005). Cyclin D1 expression was significantly correlated with lymph nodes metastasis and tumor grade. The positive rates of Cyclin D1 was significantly higher in the lymph nodes metastasis group and moderately or poorlydifferentiated than those in no lymph nodes metastasis group and welldifferentiated. A significant correlation was found between the expressions of STAT3 and Cyclin D1 in cholangiocarcinoma(P＜005). The survival time of negative expression of STAT3 and Cyclin D1 group was longer than that of positive expression of STAT3 and Cyclin D1 group(P＜001).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓STAT3 and Cyclin D1 may be important biomarkers in carcinogenesis and progression of cholangiocarcinoma.

Key words: bile duct neoplasms, activating transcription factor 3； Cyclin D1