Abstract: ［Abstract］〓Objective〖HTSS〗〓To investigate the role and mechanism of miR122 in the progression of liver cancer.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The regulatory effect of miR122 on LMNB2 gene was confirmed by luciferase reporter gene assay and Western blot. Then the effect of LMNB2 gene on the bioactivity of hepatocellular carcinoma（HCC） cells was analyzed by colony formation assay and transwell cell invasion assay .
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The luciferase activity of the wildtype mir122 mimics was lower than that of the control group(P＜005). The luciferase activity of the mutant transfected with mir122 mimics was not significantly different from that of the control group(P＞005). The expression of mir122 was silenced in hepatocellular carcinoma cells Hep3B, and the expression level of LMNB2 was significantly higher than that of the control group(P＜005). LMNB2 expression level was significantly lower than that of the control group after overexpression of mir122 in liver cancer SMMC7721 cells(P＜005). LMNB2 was overexpressed in hepatocellular carcinoma cells Hep3B, and the invasion and migration ability of hepatocellular carcinoma cells was significantly stronger than that of the control group(P＜005). The LMNB2 expression was silenced in SMMC7721 HCC cells, and the invasion and migration ability of HCC cells was significantly lower than that of the control group(P＜005). LMNB2 was overexpressed in hepatocellular carcinoma cells Hep3B, and the proliferation capacity of hepatocellular carcinoma cells was significantly stronger than that of the control group(P＜005). The LMNB2 expression was silenced in SMMC7721 hepatocellular carcinoma cells, and the proliferation capacity of hepatocellular carcinoma cells was significantly lower than that of the control group(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓miR122 can inhibit the biological activity of HCC cells by targeting LMNB2, which can be used as an early diagnostic indicator and therapeutic target for HCC.

Key words: liver neoplasms, microRNAs, gene expression