Abstract: ［Abstract］Objective〖HTSS〗To investigate the expression of miR-140-5p in prostate cancer cells and explore the biological functions of miR-140-5p in prostate cancer progression.
Methods〖HTSS〗RT-qPCR was performed to identify the expression of miR-140-5p in PC3 and DU145 cell lines. CCK8 assay and AnnexinV-FITC/PI stain were used to exam cell proliferation cell apoptosis. TCGA database was used to analyze the relationship between miR-140-5p expression level and survival of prostate cancer patient.
Results〖HTSS〗miR-140-5p was downregulated in PC3 and DU145 cells.Transfection of miR-140-5p mimic to PC3 cells significantly increased miR-140-5p level in PC3 cells. Overexpression of miR-140-5p in PC3 cells increased the cell apoptosis and decreased cell viability. Transfection of miR-140-5p inhibitor to PC3 cells significantly decreased miR-140-5p level in PC3 cells. Knockdown of miR-140-5p in PC3 cells decreased cell apoptosis and increased cell viability. Lower miR-140-5p level of prostate cancer patients had a poor overall survival analyzing from TCGA database.
Conclusion〖HTSS〗miR-140-5p is downregulated in prostate cell lines and correlates with poor survival of prostate cancer. Overexpression of miR-140-5p in PC3 cells promotes cell apoptosis and inhibits cell growth.

Key words: prostatic neoplasms, microRNA, apoptosis, cell proliferation