Abstract: Objective To investigate the regulation of interleukin-24 (IL-24 )indouble-stranded RNA-dependent protein kinase(PKR),eukaryotic initiation factor 2α(eIF-2α)-induced apoptosis of glioma cells.Methods Adenovirus Ad5F35-IL24(Ad-IL-24)and the control virus Ad5F35-enhanced green fluorescent protein (Ad-EGFP)were transfected into U87 and U251 glioma cells.Then,U87 and U251 glioma cell apoptosis were tested using flow cytometry,and the expression of PKR,phosphorylation-PKR(pPKR),eIF-2αand phosporylation eIF-2α(peIF-2α)in U87 and U251 of different groups were detected by western blot.Results The cell apoptosis rates were significantly increased to 12.3% and 13.0% in U87 and U251 glioma cells after being transfected with IL-24,and the expression of PKR,pPKR,eIF-2α and peIF-2α increased after being transfected with IL-24 in glioma cells.Conclusion IL-24 may regulate PKR,eIF-2αexpression and activation to promote apoptosis of glioma cells.

Key words: glioma, interleukins, eIF-2 kinase