Abstract: ［Abstract］  Objective〖HTSS〗〓To investigate the effect of microRNA205 and aspartatespecific caspase3 expression on the biological behavior of glioma and its possible mechanism.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The expression of miR205 in 30 cases of glioma tissue and normal brain tissue was detected by realtime fluorescence quantitative PCR. The miR205 mimics, control mimics, miR205 inhibitor(miR205inhibitor) was introduced into U251 cells and the invasive ability of the cells was observed by Transwell assay. The effect of miR205 on caspase3 was tested by bioinformatics and fluorescent protein reporter assay. The expression level of caspase3 protein and the number of cell clones in U251 cells were studied by RNA interference technique.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The expression of miR205 in glioma tissue was lower than that in normal brain tissue(P＜005), and 28 cases of glioma tissue were 28 times lower than that of miR205. The miR205 mimetic or miR205 inhibitor was transfected into U251 cells and found that miR205 mimics were significantly higher than those of control at miR205 expression levels, and that of miR205 was lower than that of control. The difference was statistically significant(P＜005). The expression of caspase3 protein in U251 cells transfected with miR205 mimics was higher than that in control transfected cells(P＜005). The fluorescence value of miR205 mimics+pcDNA3Wt cotransfection group was significantly higher than that of control and miR205 mimics+pcDNA3Mut(P＜005). The miR205 mimetic group was transfected into miR205 mimetic group, and its cell clone formation was significantly less than that of the control group and the blank group. After transfection of miR205 inhibitor, the cell clone formation was more than that in the blank group(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Most of the gliomas have miR205 expression downregulation, and miR205 may influence the invasion, proliferation and clonal ability of glioma cells by targeting caspase3, which will provide a powerful basis for further understanding and treatment of glioma.

Key words: glioma, microRNAs, caspase3