Abstract: ［Abstract］ Objective〖HTSS〗〓To investigate the effects of Baicalin on the differentiation of human umbilical cord mesenchymal stem cells(hUMSCs) into nerve cells.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The cultured P4 generation was selected as the study subjects and divided into four groups according to the drug administration:25 μg/mL group, 50 μg/mL group, 100 μg/mL group and control group. Cell morphology was observed dynamically, and the expression of neural stem cell marker, Nestin, astrocyte marker, glial fibrillary acid protein(GFAP), and neuronal marker, neuronspecific enolase(NSE) were detected by immunohistochemistry staining daily in each group. 
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The cultured P4 generation was selected as the study subjects and divided into four groups according to the drug administration: 25 μg/mL group, 50 μg/mL group, 100 μg/mL group and control group. Cell morphology was observed dynamically, and the expression of neural stem cell marker, Nestin, astrocyte marker, GFAP, and neuronal marker, NSE were detected by immunohistochemistry staining daily in each group.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Baicalin can induce HUMSCs differentiating into neurolike cells in vitro, and the expression of neural markers Nestin, NSE and GFAP showed an increasing tendency follow the concentration increasing in the range of 25-100 μg/mL of Baicalin.

Key words: mesenchymal stem cells, Baicalin, neuronlike cells