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Table of Content

    25 February 2020, Volume 41 Issue 2
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    Survey on the prevalence of thyroid nodules among different sexes in rural population in Longnan area of Gansu
    JING Gao-jing1, FU Song-bo1, JIAO Cai-hong2, TANG Xu-lei1*, TENG Wei-ping3, SHAN Zhong-yan3
    2020, 41(2):  128-131,137.  doi:10.3969/j.issn.1007-3205.2020.02.002
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    〗［Abstract］ Objective To investigate the prevalence of thyroid nodules in rural population in Longnan area of Gansu.
    Methods A stratified cluster sampling method was used to conduct a cross-sectional survey of the area. We analyzed the detection rate of thyroid nodules in different gender, age, and thyroid function groups, and used Logistic regression to analyze the risk factors of thyroid nodules.
    Results A total of 214 thyroid nodules were detected in 1 289 subjects in Longnan, Gansu. The overall prevalence was 16.60%(214/1 289), and the prevalence of male and female were 13.04%(84/644) and 20.16%(130/645), and the prevalence of female significantly higher than male, the difference was statistically significant(P＜0.05). With the increased of age, the prevalence of thyroid nodules in male and female increased gradually(P＜0.05), and the prevalence of female was significantly higher in men≥60 years(P＜0.05). In male and female, the detection rates of nodules in the abnormal thyroid function group were significantly higher than in the normal thyroid function group, and the differences were statistically significant(P＜0.05). Logistic regression showed that age≥ 60 and smoking were risk factors for thyroid nodules.
    Conclusion The detection rate of thyroid nodules in women in Longnan region of Gansu is higher than that of men, and it gradually increases with age. Abnormal thyroid function may affect the occurrence of thyroid nodules
    Effects of low carbohydrate diet intervention on leptin gene and TNF-α gene methylation in overweight patients with type 2 diabetes mellitus
    LIU Qiao-rui, JIANG Yan-fei, ZHENG Xu-lei, MA Cong, WU Li-ming, LIU Zhi-wen*
    2020, 41(2):  132-137.  doi:10.3969/j.issn.1007-3205.2020.02.003
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    ［Abstract］ Objective To investigate the effect of low carbohydrate diet intervention on the methylation of leptin gene and tumor necrosis factor-α gene(TNF-α) in overweight patients with diabetes mellitus type 2(T2DM).
    Methods One hundred and twenty  overweight patients with T2DM, were randomly divided into observation group and control group with 60 cases in each group. The observation group was given low-carbohydrate diet and the control group was given daily diet. The levels of fasting plasma glucose(FPG), fasting insulin(FINS), homeostasis model assessment(HOMA-IR), hemoglobin A1c(HbA1c), total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), body mass index(BMI), waist circumference(WC), Diabetes Distress Scale(DDS), Audit of Diabetes-dependent Quality of Life(ADQoL), serum concentrations of leptin and TNF-α, the methylation rates of leptin gene and TNF-α gene were observed before and after treatment.
    Results The levels of FPG, FINS, HOMA-IR and HbA1c after treatment were significantly decreased than those before treatment in both groups(P＜0.05). After treatment, the levels of FPG, FINS, HOMA-IR and HbA1c in observation group were significantly lower than those in control group(P＜0.05). In observation group, the levels of TC and TG after treatment were significantly decreased and HDL-C after treatment was significantly increased than those before treatment(P＜0.05). After treatment, TC and TG concentration in observation group were significantly lower and HDL-C concentration in observation group were significantly higher than those in control group after treatment(P＜0.05). In observation group, the levels of BMI, WC and DDS score were significantly lower and ADQoL score was significantly higher than those before treatment(P＜0.05). In observation group, the levels of BMI, WC and DDS score after treatment were significantly lower and HDL-C concentration was much higher than those in control group after treatment(P＜0.05). The methylation rates of leptin gene and TNF-α gene after treatment were significantly increased and the serum concentration of leptin and TNF-α after treatment were decreased significantly than those before treatment in both groups(P＜0.05), and those changes in observation group were more significant than those in control group, with the higher methylation rates of leptin gene and TNF-α gene, the lower serum concentration of leptin and TNF-α in observation group than those in control group(P＜0.05).
    Conclusion The diet intervention with low carbohydrate has significant curative effect on overweight type 2 diabetes, which can alleviate the disorder of carbohydrate and lipid metabolism, and it may be related to the methylation of leptin gene and TNF-α gene.
    Correlation between plasma arteriosclerosis index and cardiovascular disease risk in obese patients with hypertension
    YANG Fei, XIAO Chun
    2020, 41(2):  138-141.  doi:10.3969/j.issn.1007-3205.2020.02.004
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    ［Abstract］Objective To investigate the association between the atherogenic index of plasma(AIP) and cardiovascular disease risk in middle-aged obese individuals.
    Methods One hundred and twenty patients with hypertension were randomly selected and divided into obese group and non-obese group according to visceral fat area. Patients were divided into high-risk group, medium risk group and low risk group according to Framingham Risk Score. AIP, age, waist-hip fat ratio, blood pressure, body mass index, blood sugar and blood lipids in each group were compared, and the correlation between AIP and cardiovascular disease risk in hypertensive obese patients was analyzed.
    Results AIP, waist-hip fat ratio, systolic blood pressure, fasting blood glucose, total cholesterol and triglyceride were significantly higher in the obese group than in the non-obese group(P＜0.05). Waist-hip fat ratio, systolic blood pressure, bady mass index and AIP in the middle risk group and the high-risk group were significantly higher than those in the low risk group, and the waist-hip fat ratio, systolic blood pressure, bady mass index and AIP in the high-risk group were significantly higher than those in the middle risk group(P＜0.05). Spearman correlation analysis showed that AIP was positively correlated with cardiovascular factors(P＜0.05).
    Conclusion All patients with hypertension and obesity have significant dyslipidemia, high AIP value and 10-year cardiovascular risk, and AIP can be an important risk factor for patients with cardiovascular disease.
    Analysis of pathogenic bacteria and causes of laparoscopic incision infection in gastrointestinal surgery#br#
    WEI Ming1, WANG Yuan-yuan1, ZHANG Xing1, LI WEI1*, YANG Jing2, DAI Yong-jun1
    2020, 41(2):  142-144,153.  doi:10.3969/j.issn.1007-3205.2020.02.005
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    ［Abstract］Objective To make targeted infection control strategies by analyzing pathogenic bacteria and risk factors of laparoscopic incision infection in gastrointestinal surgery.
    Methods The infection of incision in laparoscopic operation of gastrointestinal surgery was statistically analyzed.
    Results A total of 21 cases of 867 patients developed wound infection, the infection rate was 2.42%. The highest infection rate was appendectomy. Pathogen analysis: 7 strains of gram-positive bacteria(33.33%), 13 strains of gram-negative bacteria(61.90%), and 1 strain of fungus(4.76%). Patients with age >60 years old, body mass index（BMI）>25, diabetes mellitus, operation time>90 minutes, sample passing at incision, no use of antibiotics, anesthesia score of grade Ⅲ-Ⅳ, abdominal infection, no incision protection, lower digestive tract at operation site and hospitalization time >8 days had higher incidence of infection. Logistic regression analysis showed that age＞60 years, BMI＞25, and those with diabetes were risk factors for laparoscopic incision infection （P＜0.05）.
    Conclusion Special attention should be paid to the prevention of incision infection in laparoscopic surgery for the elderly, obese and diabetic patients.
    Effect of low-approach microsurgical varicocelectomy and high-approach laparoscopic varicocelectomy and the influence on hemodynamics#br#
    YANG Qiang, WEI Shou-yuan， ZHENG Pei
    2020, 41(2):  145-148.  doi:10.3969/j.issn.1007-3205.2020.02.006
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    〗［Abstract］ Objective To analyze the effect of low-approach microsurgical varicocelectomy and high-approach laparoscopic varicocelectomy and the influence on hemodynamics and semen quality.
    Methods One hundred and four varicocele patients were randomly divided into control group and observation group, 52 cases in each group. The control group was treated with high-approach laparoscopic varicocelectomy, the observation group was treated with low-approach microsurgical varicocelectomy. The time of operation, the time of hospitalization after operation, the incidence of complications and the rate of recurrence of two groups were compared. The hemodynamic parameters of the testicular arteries and semen quality before and 6 months after the operation between two groups were compared.
    Results The operation time of observation group was significantly longer than control group, the time of hospitalization after operation was significantly lower than control group（P＜0.05）. The resistance index(RI), pulse index(PI), and peak systo lic velocity(PSV) of both groups were decreased and that of observation group were significantly lower than control group 6 months after operation(P＜0.05). The number of sperm, sperm concentration and sperm motility of the two groups were significantly increased 6 months after operation(P＜0.05), and that of the observation group was significantly higher than the control group(P＜0.05). The incidence of complications and the rate of recurrence of observation group were significantly lower than control group.
    Conclusion Low-approach microsurgical varicocelectomy can effectively treat varicocele, reduce the incidence of complications and recurrence, reduce the resistance of the testicular blood flow, improve the hemodynamics and improve the quality of the semen. It is safe and worthy of clinical promotion.
    Application effect and long-term follow-up analysis of dynamic external fixator for complex fracture of elbow joint
    LI Yun-jin, HUANG Ping, HUANG Dong-yong, ZHANG Yan-qing, YE Xiao-yan
    2020, 41(2):  149-153.  doi:10.3969/j.issn.1007-3205.2020.02.007
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    〗［Abstract］ Objective To evaluate the application effect of dynamic external fixation in elbow fractures.
    Methods Sixty patients with hyperelbow fracture were randomly divided into control group and experimental group with 30 cases in each group. Internal fixation plate screw wire and external fixator were used in the experimental group and the control group was fixed with internal fixation steel plate screw wire and external fixator. Visual Analogue Scale（VAS） score, operation duration time, fracture healing time, bone nonunion in 1 month after operation were compared. Mayo elbow function scores were followed-up at 1, 3, 6 and 12 months and scores were compared between two groups.
    Results VAS score and fracture healing time of the experimental group were significantly shorter than those of the control group(P＜0.05), and there were no statistically significant differences in operation duration time and bone nonhealing rate between two groups(P＞0.05).The elbow function scores of the two groups showed a gradually increasing trend after surgery, but there was a large increase in the experimental group. The Mayo elbow function score of the two groups showed statistically significant differences in the interaction of groups, time points and between groups and time points(P＜0.05).There was no significant difference in the incidence of surgical complications between two groups within 3 months after surgery(P＞0.05).
    Conclusion The application effect of external dynamic fixed stent for complex fracture of elbow joint is better, which can significantly improve the function of the elbow, reduce the healing time of the fracture, reduce the VAS score and make Mayo elbow function score higher. It is worthy of clinical promotion and application.
    The protective effect of ultrasonic scalpel combined with cosmetic incision on facial nerve in surgery for parotid neoplasms
    GU Zhan-guo, SHI Fu-min, ZHAI Jian, HAN Xiao-chen, LI Jun, ZHAO Xiu-li
    2020, 41(2):  154-157,162.  doi:10.3969/j.issn.1007-3205.2020.02.008
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    ［Abstract］Objective To investigate the protective effect of ultrasonic scalpel combined with cosmetic incision on facial nerve in surgery for parotid neoplasms.
    Methods One hundred and fifty patients with parotid neoplasms were selected and they were divided into observation group and control group by the random number table method with 75 cases in each group. The observation group was treated with ultrasonic scalpel combined with cosmetic incision, while the control group was treated with traditional electrotome combined with cosmetic incision. The indexes during and after surgery, facial nerve electromyogram, grade of facial nerve function, complications after surgery and tumor recurrence rate were compared between the two groups.
    Results The surgical time, intraoperative blood loss, postoperative drainage volume and hospitalization time of the observation group were significantly shorter or less than those of the control group(P＜0.05). The wave amplitude in observation group at 30 days after surgery was significantly higher than that in control group while the degeneration rate of nerve injury was significantly lower than that in control group(P＜0.05). There was no significant difference in grade of facial nerve function between two groups at 30 days after surgery(P＞0.05). The incidence rates of temporary facial paralysis and earlobe numbness in observation group were significantly lower than those in control group(P＜0.05).
    Conclusion Ultrasonic scalpel has good hemostatic and cutting function. Its combined use with cosmetic incision can reduce the facial nerve injury in resection of parotid neoplasms, shorten the surgical time and accelerate the rehabilitation process.
    Effect of Aspirin combined with Fentanyl on the prognosis of patients with severe uterine cavity adhesionsurgery
    LI Li1, WANG Min2
    2020, 41(2):  158-162.  doi:10.3969/j.issn.1007-3205.2020.02.009
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    ［Abstract］ Objective To study the effect of Aspirin combined with Femoston on the improvement rate of menstruation, uterine artery blood flow and endometrial repair in patients after severe intrauterine adhesions surgery.
    Methods One hundred and eight  women of childbearing age with severe intrauterine adhesions were randomly divided into observation group and control group with 54 cases in each group. All patients in the two groups underwent uterine adhesions separation surgery. The patients in control group were treated with Fenmotong orally after operation. On this basis, the observation group was given Aspirin for a long period of one month. The clinical efficacy and total effective rate of the two groups were observed. Pregnancy status, endometrial thickness, menstrual volume and uterine artery blood flow parameters of the two groups were compared.
    Results The total effective rate of clinical efficacy and uterine cavity shape recovery in the observation group was significantly higher than that in control group(P＜0.05). Nineteen cases were pregnant in observation group, 13 cases were successful(68.43%) and 15 cases in the control group, 5 cases were successful(33.34%). The pregnancy success rate in observation group was higher than that in control group(P＜0.05). After treatment, the endometrial thickness and menstrual blood volume of the two groups were significantly improved compared with those before treatment(P＜0.05). The improvement of endometrial thickness and menstrual blood volume in the treatment group was higher than that before treatment and in control group(P＜0.05). Resistance index（RI） and pulse index（PI）  of uterine artery blood flow parameters in two groups were lower than before treatment, vascularization index（VI）, vascularization flow index（VFI） and flow index(FI) were higher than before treatment, RI and PI in observation group were lower than that in control group after treatment, VI, VFI and FI in observation group were higher than that in control group after treatment(P＜0.05). There was no significant difference in adverse reactions(P＞0.05).
    Conclusion The combination of Aspirin and Finchton can promote the repair of endometrium, improve the situation of menstruation and the blood flow of the arteries after severe intrauterine adhesion, which is safe.
    Clinical analysis of laparoscopic uterine artery occlusion plus uterine adenomyectomy combined with Mirena in the treatment of adenomyoma#br#
    MI Gui-lan, SHU Zhi-ming， WU Jian-fa， LIU Zhou*
    2020, 41(2):  163-166,171.  doi:10.3969/j.issn.1007-3205.2020.02.010
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    ［Abstract］ Objective To observe the efficacy of laparoscopic uterine artery occlusion plus uterine adenomyectomy combined with Mirena on uterine adenomyoma and its effect on ovarian function.
    Methods One hundred and thirty patients with adenomyoma were randomly divided into observation group and control group, 65 cases in each group. The control group was given Mifepristone, and the observation group was treated with Mirena. The clinical efficacy and total effective rate were observed in two groups. The uterus volume, uterine cavity size and carbohydrate antigen 125（CA125） were compared between two groups. The menstrual and ovarian function was compared before and after treatment, and the incidence of adverse reactions was compared between two groups.
    Results The clinical efficacy and total effective rate in observation group were higher than those in control group(P＜0.05). The uterus volume, uterine cavity size and CA125 level in two groups were significantly smaller and lower than those before treatment(P＜0.05), and the reduction and reduction in the observation group was more obvious. After treatment, the uterus volume, uterine cavity size and CA125 level in observation group were lower than those in control group(P＜0.05). After treatment, the menstrual period was significantly shortened, the pain score was significantly lower, and the menstrual cycle was significantly longer than that before treatment(P＜0.05). After treatment the pain score in observation group was lower than that of the control group(P＜0.05), the levels of luteinizing hormone（LH） and follicle-stimulating hormone（FSH） in two groups were significantly lower than those before treatment, and the estradiol（E2） level was significantly higher than that before treatment(P＜0.05). The levels of LH, FSH and E2 in the observation group were lower than those in control group(P＜0.05). There was no significant difference in the incidence of adverse reactions between two groups(P＞0.05).
    Conclusion Laparoscopic uterine artery occlusion plus resection of adenomyoma combined with Mirena is effective in treating adenomyoma of the uterus, which can significantly relieve symptoms and improve the body′s sex hormone disorder.
    The correlation between the expression of miR-141-3p and Cisplatin chemosensitivity of ovarian cancer cells#br#
    MU Wei-hong, CHEN Wen-qi, PENG Yuan-yuan, ZHANG Jing*
    2020, 41(2):  167-171.  doi:10.3969/j.issn.1007-3205.2020.02.011
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    ［Abstract］ Objective To investigate the expression and role of miR-141-3p in Cisplatin resistance of ovarian cancer cells.
    Methods RT-qPCR was performed to identify the expression of miR-141-3p in A2780/DDP cells. CCK8 assay was used to exam cell proliferation. Western blot analysis was used to detect proliferating cell nuclear antigen（PCNA） protein level.
    Results miR-141-3p was upregulated in DDP-resistance A2780/DDP cells compared with DDP-sensitive A2780 cells（P＜0.05）. Overexpression of miR-141-5p in A2780 cells increased the IC50 of DDP compared with negative control, and also increased PCNA protein expression（P＜0.05）. Knockdown of miR-14-5p in A2780/DDP cells decreased the IC50 of DDP compared with negative control, and also decreased PCNA protein expression（P＜0.05）.
    Conclusion The expression of miR-141-3p increased in DDP-resistance of ovarian cancer. Silencing of miR-141-3p can increase the chemo-sensitivity in ovarian cancer cells to DDP.
    Effects of Dipyridamole and Tacrolimus on high coagulation state and serum sICAM-1 in children with steroid-resistant Henoch-Schonlein purpura#br#
    JIANG Chen-xi， WANG Xue-ping， WU Chun-qing
    2020, 41(2):  172-175,180.  doi:10.3969/j.issn.1007-3205.2020.02.012
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    ［Abstract］ Objective To investigate the effects of dipyridamole and tacrolimus on high coagulation state and serum soluble intercellular adhesion molecule-1(sICAM-1) in children with steroid-resistant Henoch-Schonlein purpura.
    Methods Ninety children with steroid-resistant Henoch-Schonlein purpura were randomly divided into control group and observation group, 52 cases in each group. The control group received heparin combined with dipyridamole on the basis of routine treatment, and the observation group were given tacrolimus on the basis of control group. The clinical effect and the time of clinical symptom disappearance between the two groups were compared. And the changes of coagulation indexes and serum level of sICAM-1 before and after treatment were detected.
    Results The total effective rate of observation group was significantly higher than that of control group(P＜0.05). The clinical symptoms of joint swelling, rash and abdominal pain in the observation group were significantly shorter than those in the control group(P＜0.05). After treatment, the D-Dimer level of the observation group was significantly lower than that in the control group, while the prothrombin time, thrombin time and activated partial thromboplastin time levels were significantly higher than those in the control group(P＜0.05). The serum level of sICAM-1 in the observation group was significantly lower than that in the control group(P＜0.05).
    Conclusion Dipyridamole combined with tacrolimus in the treatment of steroid-resistant Henoch-Schonlein purpura children is significantly effective, it can significantly improve the blood coagulation index, inhibit the immune response, reduce serum sICAM-1, promote the remission of children′s symptoms.
    Effect of polypolycarnitol combined with Atenolol in treatment of infantile hemangioma in parotid region and its effect on COX-2,CTGF#br#
    LI Lei1, LIANG Wei1, CHEN Chen2, ZHANG Hong-peng3
    2020, 41(2):  176-180.  doi:10.3969/j.issn.1007-3205.2020.02.013
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    ［Abstract］ Objective To study the effect of polypolycarnitol combined with Atenolol in treatment of infantile hemangioma in parotid region and its effect on cyclooxygenase 2(COX-2), connective tissue growth factor(CTGF), vascular endothelial growth factor（VEGF）.
    Methods Seventy-six patients with hemangioma of parotid gland were randomly divided into control group(38 cases) and observation group(38 cases). In the control group, the children were treated with atenolol, and the observation group was given a local injection of 1% polykarl in the tumor. The clinical efficacy and adverse reactions were compared between two groups.The expression of COX-2, CTGF and VEGF and immune function were compared between two groups of children before and after treatment.
    Results The two groups took atenolol for an average of 6 months, and the observation group received 1% polycarnitol for 3 courses on average.The treatment was completed in 76 children, followed up for 12 months without loss of follow-up. The total effective rate of observation group was significantly higher than that of control group(P＜0.05). After treatment, the positive rate of COX-2,CTGF and the level of VEGF in observation group were significantly lower than those in control group. The levels of IgA,IgG and IgM in observation group were significantly higher than those in control group(P＜0.05). During the treatment period, compared with the control group, the adverse reactions in observation group were significantly lower(P＜0.05).
    Conclusion Atenolol combined with polycarnitol is effective and safe in the treatment of infantile hemangioma in parotid region. It can inhibit the expression of COX-2, CTGF and VEGF.
    Clinical efficacy on Kangfuxin Liquid combined with Minocycline in treatment of chronic periodontitis and its effect on expression of PD-1 and PD-L1#br#
    WANG Zhong-chao1， FAN Li-yuan2*， SUN Xiao-juan1， TAN Hong1， ZHOU Cong1， WU Bao-qin1
    2020, 41(2):  181-184,190.  doi:10.3969/j.issn.1007-3205.2020.02.014
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    ［Abstract］ Objective To explore the clinical efficacy on Kangfuxin liquid combined with Minocycline in treatment of chronic periodontitis and its effect on expression of programmed death-1（PD-1） and programmed death-ligand 1（PD-L1）.
    Methods One hundred and ninety-two patients with periodontitis were randomly divided into two groups, 96 cases in each group. the observation group was treated with Minocycline combined with Kangxinxin liquid, the control group was treated with Minocycline. The clinical efficacy and total effective rate of the two groups were observed. The plaque, periodontal probing depth and gingival bleeding index of the two groups were compared. The changes of serum inflammatory factors before and after treatment were compared. The serum PD-1 and PD-L1, level changes and adverse reactions before and after treatment were compared.
    Results The clinical efficacy and total effective rate of the observation group were higher than those of the control group(P＜0.05). The plaque index, periodontal probing depth and gingival bleeding index of the observation group were lower than the control group(P＜0.05). After treatment, the serum levels of interferon-γ（IFN-γ）, tumor necrosis factor-α（TNF-α）, interleukin-8（IL-8） and hypersensitive C-reactive protein（hs-CRP） were significantly lower than those before treatment(P＜0.05), and IL-10 was significantly higher than that before treatment(P＜0.05). The observation group decreased and increased greatly. The serum levels of IFN-γ, TNF-α, IL-8 and hs-CRP in the observation group were lower than those in the control group(P＜0.05). There was no significant difference in the incidence of adverse reactions between two groups(P＞0.05).
    Conclusion Minocycline combined with Kangxinxin can effectively reduce the expression of PD-1 and PD-L1 on CD4+ and CD8+ T lymphocytes in patients with chronic periodontitis, relieve inflammation, improve clinical symptoms, and improve treatment efficacy.
    Diagnostic value of DTI in cervical spondylotic myelopathy
    LI Ming, GAO Xian-da, DING Wen-yuan， YANG Da-long, SUN Jia-yuan, MA Lei*
    2020, 41(2):  185-190.  doi:10.3969/j.issn.1007-3205.2020.02.015
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    ［Abstract］Objective To explore the diagnosis value of diffusion tensor imaging in patients with cervical spondylotic myelopathy.
    Methods Thirty-seven patients with cervical spondylotic myelopathy were recruited in this study and anterior cervical discectomy and fusion were performed in all patients. According to preoperative different degree of signal intensity ratio(SIR), patients were divided into three groups: 11 cases in group A SIR<1.32, 12 cases in group B SIR 1.32-1.68, 14 cases in group C SIR>1.68. Clinical information, FA value, Japanese Orthopaedic Association(JOA) scores and detailed limb function scores were collected to compared among three groups. The correlation between JOA scores and FA value was identified by Pearson correlation analysis.
    Results Operations were successfully performed in all patients of three groups. There was no significant difference in surgical time, bleeding, rate of cerebrospinal fluid leakage among three groups(P＞0.05). The FA value was lower in group C than in group A and B(P＜0.05). JOA score was higher in last follow-up visit than preoperative in three groups, while it was lower in group C than group A and B at both preoperative and last follow-up visit. The JOA scores improvement rate was lower in group C than group A(P＜0.05). Walking time of 6 m of normal step was shorter in follow up than preoperative in three groups(P＜0.05), while it was longer in group C than group A and B at both preoperative and follow up. Walking time of 6 m of maximum step was longer in group B than group A before operation and at last follow-up visit, it was longer in group B than group A. In three groups, walking time of 6 m of maximum step was shorter at last follow-up visit than preoperative(P＜0.05). Before operation, five consecutive sitting time was longer in group C than group A and B. At last follow-up visit, five consecutive sitting time in group B was longer than group A and it was longer in group C than groups A and B. Five consecutive sitting time was shorter in last follow-up visit than preoperative in three groups(P＜0.05). In three groups, times of continuously hold loose fist in 10 s was greater at last follow-up visit than preoperative(P＜0.05), while at preoperative and last follow-up visit, times of continuously hold loose fist in 10 s was less in group C than group A. There was positive correlation between preoperative JOA scores(P＜0.05), JOA scores improvement rate(P＜0.05) and FA value.
    Conclusion FA value shows satisfactory diagnosis value in cervical spondylotic myelopathy patients with increased SIR in cervical spinal cord. FA value is closely related to clinical outcomes after surgery.
    High-resolution MRI morphology of dominant hemisphere insular lobe in Chinese population#br#
    WANG Jia-qi1, LIU Li-ping1, ZHANG Xu1, LIU Huai-jun2, TIAN Xin2*
    2020, 41(2):  191-195.  doi:10.3969/j.issn.1007-3205.2020.02.016
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    ［Abstract］ Objective To establish the normal values of insular lobe morphology in Chinese people, for diagnosing insular psychiatric diseases.
    Methods A total of 75 healthy volunteers were enrolled in the study. MRI of the head were performed to measure and analyze the morphological data of the subtropical lobes, including the subcortical, white matter, and sulcus width values. Morphological data was compared and analyzed.
    Results The length of transverse sulcus had significant difference among different age groups(P＜0.05). There was no significant difference in the width of subcortex, white matter and sulcus among the other dominant cerebral hemispheres(P＞0.05).
    Conclusion The insular lobe belongs to the lower level of brain lobe. Its morphological parameters are less affected by age and the measured values are stable. Therefore, it may be a reliable index for the study of brain mental disorders.
    Relationship between the characteristics of CT, MRI and three-dimensional power Doppler ultrasound in PCOS patients and endocrine functions#br#
    XU Li, ZHAO Bo, ZHAO Ping
    2020, 41(2):  196-199,218.  doi:10.3969/j.issn.1007-3205.2020.02.017
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    ［Abstract］Objective To investigate the relationship between CT, MRI and three-dimensional power Doppler ultrasound in patients with polycystic ovary syndrome polycystic ovary syndrome(PCOS) and its pathological type and endocrine function.
    Methods A total of 128 PCOS patients were enrolled as the PCOS group. Fifty healthy subjects were selected as the control group. Three-dimensional power Doppler ultrasonography was used to observe the characterization of all subjects. The area total area(TA), ovarian volume(2D-OV), interstitial area(SA), ovarian interstitial pulsation index(PI) and resistance index（RI) were compared between two groups; the luteinizing hormone（LH）, follicle stimulating hormone（FSH）, LH/FSH, testosterone（T）, estradiol（E2） and Homeostasis model-insulin resistance（HOMA-IR） were detected and compared between two groups. The relevance of ultrasound parameters and pathological classification of patients were analyzed. The PCOS patients were further divided into CT group and MRI group according to the random number table method, with 64 patients in each group. The CT and MRI were performed in CT group and MRI group, respectively. The differences between the two groups were compared.
    Results TA, SA and 2D-OV in PCOS group were significantly higher than those in control group, PI and RI were significantly lower than those in control group(P＜0.05). The levels of LH, LH/FSH, T, E2 and HOMA-IR in PCOS group were significantly higher than those in the control group(P＜0.05). 2D-OV was positively correlated with LH/FSH, HOMA-IR, TA, SA was positively correlated with T(P＜0.05). Logistic regression analysis showed that TA,2D-OV,PI,RI were independent risk factors affecting the pathological classification of PCOS. The performance characteristics of CT and MRI detection were significant difference.
    Conclusion There are significant differences in the ultrasound characteristics and endocrine indexes between PCOS patients and control group, and there is a certain correlation between them. The ovarian ultrasound characteristics can reflect the endocrine function of patients to some extent, and it is of great significance for judging the pathological classification of patients.
    JNK3 overexpression promoted the progression of myocardial ischemia reperfusion injury
    HUANG Ji-xia1, GAO Peng-zhi2*, ZHAO Chen1, LI Xiao-na2, WEI Wei2, NIU Xiao-lei2
    2020, 41(2):  200-203.  doi:10.3969/j.issn.1007-3205.2020.02.018
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    ［Abstract］Objective To analyze the relationship between c-Jun N-terminal kinase 3(JNK3) expression and myocardial ischemia reperfusion injury. To investigate the effect of JNK3 overexpression on the patients with myocardial ischemia reperfusion injury.
    Methods Seventy-five patients with myocardial ischemia reperfusion injury were selected.The expression of JNK3 mRNA in peripheral blood of patients was detected, and the high expression rate of JNK3 mRNA in different clinicopathological groups was compared.
    Results The high expression rate of JNK3 mRNA in the <50-year-old group was higher than that in the ≥50-year-old group, the high expression rate of JNK3 mRNA in the reperfusion arrhythmia group was higher than that in the non-arrhythmia group, the high expression rate of JNK3 mRNA in the group with abnormally elevated myocardial enzyme was higher than that in the group without abnormally elevated myocardial enzyme, the difference was statistically significant(P＜0.05). There was no significant difference in the high expression rate of JNK3 mRNA between different genders(P＜0.05).
    Conclusion JNK3 was overexpressed in patients with myocardial ischemia-reperfusion injury with reperfusion arrhythmia and abnormally elevated myocardial enzymes, JNK3 can be used as a therapeutic target for patients with myocardial ischemia-reperfusion injury.
    The application value of expression of serum BDNF combined with ambulatory electroencephalogram in children with febrile convulsion
    FENG Yan， JIANG Shan， MA Jin， ZHANG Xuan
    2020, 41(2):  204-207,213.  doi:10.3969/j.issn.1007-3205.2020.02.019
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    ［Abstract］Objective To discuss the expression of serum brain derived neurotrophie factor(BDNF) and the application value of BDNF combined with ambulatory electroencephalogram(AEEG) in children with febrile convulsion, providing dependable basis in clinical diagnosis and treatment.
    Methods One hundred children with febrile convulsions and 50 healthy children who underwent physical examination at the same time were selected to detect and analyze the serum BDNF level and AEEG of all the selected children.
    Results The serum BDNF levels of the simple febrile convulsion group and the complex febrile convulsion group were significantly higher than that of the control group, and the serum BDNF of the complex febrile convulsion group was significantly higher than that of the simple febrile convulsion group(P＜0.05). The incidence of AEEG abnormality in complex febrile convulsion group was significantly higher than that of the simple febrile convulsion group(P＜0.05). But there was no statistical difference in phases and parts distribution of AEEG abnormalities between complex febrile convulsion group and simple febrile convulsion group(P＞0.05). In one year, the incidence of epilepsy in the complex febrile convulsion group was significantly higher than that of the simple febrile convulsion group(P＜0.05). The specificity of BDNF+AEEG in diagnosis of febrile convulsion was significantly higher than that of BDNF and AEEG(P＜0.05). ROC curve analysis showed that the area of BDNF+AEEG was significantly higher than that of BDNF and AEEG(P＜0.05).
    Conclusion The combined application of serum BDNF expression level and AEEG has certain value in clinical diagnosis and treatment in febrile convulsion children, and it is worthy of clinical application.
    Effect of chemotherapy on the expression of NK cell receptor in peripheral blood of patients with non-small cell lung cancer and its clinical significance
    LI Li-fang1, DAI Xian-li2, HOU Zhi-hua2, WU Shu-cai2， WANG Zhi-hua3, LIU Xin-yan2*
    2020, 41(2):  208-213.  doi:10.3969/j.issn.1007-3205.2020.02.020
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    ［Abstract］Objective To investigate the changes and clinical significance of natural killer（NK） cell activating receptors and inhibitory receptors in patients with non-small cell lung cancer before and after chemotherapy.
    Methods Fifty patients with non-small cell lung cancer(25 cases each in stages Ⅰ- ⅢA and ⅢB- Ⅳ) and 10 healthy subjects(control group) were selected. Flow cytometry was used to detect peripheral blood NK cell activating receptors NKG2D, NCRs(NKp30, NKp44, NKp46) and inhibitory receptors CD158a, CD158b, CD158e, and receptor expression after two cycles of chemotherapy of stages ⅢB to Ⅳ. CD56dim and CD56bright were counted according to the expression intensity of CD56.
    Results The CD56dim activated receptors NKG2D and NKp30 in patients with stage ⅢB-Ⅳ in non-small cell lung cancer were lower than those in healthy subjects and patients in stage Ⅰ-ⅢA, the expression of CD56dim activated receptor NKp44 of non-small cell lung cancer  in stage Ⅰ- ⅢA and  ⅢB- Ⅳ was lower than that of healthy subjects(P＜0.05). There was no significant difference between three groups in CD56dim activated receptor NKp46(P＞0.05). There was no significant difference between the three groups in the expression of NKG2D, NKp30, NKp44 and NKp46(P＞0.05). There was no significant difference in the CD56dim inhibitory receptors CD158a, CD158b, CD158e and CD56bright inhibitory receptors CD158a, CD158b, and CD158e in three groups(P＞0.05). Due to the lack of enrolled staff, 14 patients were enrolled after two cycles chemotherapy of stage ⅢB to Ⅳ. The percentages of CD56dim activated receptors NKp30 and NKp44 in patients with stage ⅢB- Ⅳ non-small cell lung cancer after chemotherapy were higher than those before treatment(P＜0.05). After chemotherapy, CD56dim activating receptors NKp46, NKG2D and inhibitory receptors CD158a, CD158b, CD158e were not statistically different from those before chemotherapy(P＞0.05). After chemotherapy, there was no significant difference in the activation receptor NKp30, NKp44, NKp46, NKG2D and the inhibition receptor CD158a, CD158b and CD158e between two groups(P＞0.05). After 2 cycles of chemotherapy, the PR rate of 14 patients reached 78.5%(11/14).
    Conclusion The decreased expression of activated receptors in CD56dim NK cells in stage ⅢB-Ⅳ may be related to the decreased cytotoxicity of activated receptors on NK cells caused by tumor microenvironment. After chemotherapy, the expression of NKp30 and NKp44 can be increased. It is speculated that chemotherapy can play its killing effect on tumor by improving the activity of NK cells.
    Clinical application of serum miRNA-494 and miRNA-125a expression in lumbar intervertebral disc degeneration disease
    SU Xiao-ming1, ZHU Chao2, YANG Yu1*, LI Yi3
    2020, 41(2):  214-218.  doi:10.3969/j.issn.1007-3205.2020.02.021
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    ［Abstract］ Objective To investigate the correlation of the serum expression of microRNA-494 and microRNA-125a with the degree of lumbar intervertebral disc degeneration.
    Methods Forty-five patients with prolapse or protrusion of lumbar intervertebral disc were selected as degeneration group and 42 patients with low back pain as control group. All patients underwent lumbar MRI. The relationship between the degree of degeneration of lumbar intervertebral disc and Pfirrmann(PM) grade of intervertebral disc was analyzed via diffusion weighted magnetic resonance imaging(DWI).
    Results In the control group, 126 intervertebral discs were examined, of which 99 were in grade Ⅰ and Ⅱ(normal), accounting for 78.6%(99/126), 27 were in grade Ⅲ, Ⅳ and Ⅴ, accounting for 21.4%(27/126).135 intervertebral discs were examined in the degeneration group, of which 66 were in grade Ⅰ and Ⅱ(normal), accounting for 12.6%(17/135),118 were in grade Ⅲ, Ⅳ and Ⅴ, accounting for 87.4%(118/135).There was significant difference in PM grading constituent ratio between two groups(P＜ 0.05), and the apparent diffusion coefficient(ADC) value in the degenerative group was lower than that in the control group(P＜0.05).The serum level of microRNA-125a in degenerative group was significantly lower whereas of serum microRNA-494 was significantly higher than that in control group(P＜0.05).The expression level of microRNA-125a in degenerative group was positively correlated with ADC value(r=0.853, P＜0.01), and the expression level of microRNA-494 was negatively correlated with ADC value(r=-0.897, P＜0.01).ROC curve analysis of microRNA-125a and microRNA-494 in serum showed that the area under the curve of microRNA-125a and microRNA-494 was 0.893(95%CI=0.815-0.971, P＜0.01) and 0.852(95%CI=0.762-0.932, P＜0.01), respectively. The sensitivity was 87.9% and 93.7%, specificity was 77.0% and 67.7%, accuracy was 80.0% and 77.7%, positive predictive value was 92.3% and 92.1%, negative predictive value was 81.8% and 76.9%, respectively.
    Conclusion The expression of miRNA-494 and miRNA-125a in serum may be related to the pathogenesis of lumbar disc degeneration disease. Detecting their expression levels can provide a genetic basis for early diagnosis and treatment of lumbar disc degeneration disease.