载脂蛋白D激活Wnt/β-catenin信号通路参与牙髓细胞增殖分化过程

doi:10.3969/j.issn.1007-3205.2024.07.018

河北医科大学学报 ›› 2024, Vol. 45 ›› Issue (7): 850-854.doi: 10.3969/j.issn.1007-3205.2024.07.018

载脂蛋白D激活Wnt/β-catenin信号通路参与牙髓细胞增殖分化过程

广东省深圳市宝安区中心医院口腔科，广东深圳 518100

出版日期:2024-07-25 发布日期:2024-07-18
作者简介:林健生（1987-），男，广东深圳人，广东省深圳市宝安区中心医院主治医师，医学学士，从事口腔科疾病诊治研究。
基金资助:
广东省自然科学基金-面上项目(2020A1515011105);深圳市宝安区科技计划-基础研究项目（20210513212310001）

Involvement of apolipoprotein D in the proliferation and differentiation of dental pulp cells by activating Wnt/β-catenin signaling pathway

Department of Stomatology, Shenzhen Bao′an District Central Hospital, Guangdong Province, Shenzhen 518100, China

Online:2024-07-25 Published:2024-07-18

摘要/Abstract

摘要： 目的探究载脂蛋白D（apoliprotein D，APOD）激活果蝇无翅基因蛋白/β-连锁蛋白（Wnt/β-catenin）信号通路参与牙髓细胞（dental pulp cells，DPCs）增殖分化过程。
方法体外培养人牙髓细胞（human dental pulp cells，HDPCs），设置空白组、APOD低剂量组、APOD高剂量组和si-APOD组。空白组于DMEM培养基培养，APOD低剂量组向培养基中加入2 nmol/L APOD，APOD高剂量组向培养基中加入32nmol/LAPOD，si-APOD组采用APOD si-RNA转染HDPCs细胞。采用RT-qPCR检测APOD mRNA表达水平，CCK-8检测细胞增殖能力，碱性磷酸酶（alkaline phosphatase，ALP）活性检测成骨分化能力，Western Blot检测Wnt/β-catenin通路蛋白表达量。
结果与空白组比较，APOD低剂量组、APOD高剂量组APOD mRNA水平及细胞活力升高，7、14 d的ALP 活性升高，Wnt5a、β-catenin蛋白上调（P＜0.05），si-APOD组APOD mRNA水平，细胞活力降低，7、14 d的ALP 活性降低，Wnt5a、β-catenin蛋白下调（P＜0.05）；与APOD低剂量组比较，APOD高剂量组APOD mRNA水平及细胞活力升高，7、14 d的ALP 活性升高，Wnt5a、β-catenin蛋白上调（P＜0.05），si-APOD组APOD mRNA水平及细胞活力降低，7、14 d的ALP 活性降低，Wnt5a、β-catenin蛋白下调（P＜0.05）；与APOD高剂量组比较，si-APOD组APOD mRNA水平及细胞活力降低，7、14 d的ALP 活性降低，Wnt5a、β-catenin蛋白下调（P＜0.05）。
结论 APOD能够促进HDPCs细胞的增殖分化，其作用机制可能与激活 Wnt/β-catenin信号通路有关。

关键词: 牙髓, 细胞增殖, 载脂蛋白D类

Abstract: Objective To investigate the involvement of apolipoprotein D (APOD) in the proliferation and differentiation of dental pulp cells (DPCs) by activating Wnt/β-catenin signaling pathway in Drosophila melanogaster.
Methods Human dental pulp cells (HDPCs) were cultured in vitro and divided into blank group, low-dose APOD group, high-dose APOD group and si-APOD group. Cells in the blank group were cultured in DMEM medium without any treatment. APOD 2 nmol/L and 32 nmol/L were added to the low-dose APOD group and high-dose APOD group, respectively. HDPCs cells in the si-APOD group were transfected with APOD si-RNA. RT-qPCR was used to detect the expression of APOD mRNA, and CCK-8 was used to detect cell proliferation. Alkaline phosphatase (ALP) activity was used to detect osteogenic differentiation, and Western Blot was used to detect Wnt/β-catenin pathway protein expression.
Results Compared with blank group, APOD mRNA level and cell viability were increased, ALP activity was increased at 7 d and 14 d, and Wnt5a and β-catenin proteins were up-regulated in low- and high-dose APOD groups (P＜0.05). In si-APOD group, APOD mRNA level and cell viability were decreased, ALP activity at 7 d and 14 d was decreased, and Wnt5a and β-catenin proteins were down-regulated (P＜0.05). Compared with the low-dose APOD group, the APOD mRNA level and cell viability were increased, the ALP activity was increased at 7 d and 14 d, and the Wnt5a and β-catenin proteins were up-regulated in the high-dose group (P＜0.05). In si-APOD group, APOD mRNA level and cell viability were decreased, ALP activity at 7 d and 14 d was decreased, and Wnt5a and β-catenin protein were down-regulated (P＜0.05). Compared with the high-dose APOD group, the mRNA level and cell viability of APOD were significantly decreased, the activity of ALP at 7 d and 14 d was decreased, and the proteins of Wnt5a and β-catenin were down-regulated in si-APOD group (P＜0.05).
Conclusion APOD can promote the proliferation and differentiation of HDPCs cells, and its mechanism may be related to the activation of Wnt/β-catenin signaling pathway.

Key words: dental pulp, cell proliferation, apolipoproteins D

林健生, 孔令佳, 鄂佳, 王利娜, 姚志文. 载脂蛋白D激活Wnt/β-catenin信号通路参与牙髓细胞增殖分化过程 [J]. 河北医科大学学报, 2024, 45(7): 850-854.

LIN Jian-sheng, KONG Ling-jia, E Jia, WANG Li-na, YAO Zhi-wen. Involvement of apolipoprotein D in the proliferation and differentiation of dental pulp cells by activating Wnt/β-catenin signaling pathway [J]. Journal of Hebei Medical University, 2024, 45(7): 850-854.

[1]	王旭, 徐静, 滑芳, 王玉净, 都治香. lncRNA SNHG12促进宫颈癌SiHa细胞迁移、侵袭和抑制细胞凋亡 [J]. 河北医科大学学报, 2023, 44(5): 547-552.
[2]	张佳慧, 马红云, 秦娟. 基于PI3K/Akt通路沉默miR-26b对妊娠期高血压胎盘滋养细胞增殖、凋亡的影响[J]. 河北医科大学学报, 2021, 42(9): 1063-1067.
[3]	殷亮亮, 李春年. EPO对低氧状态下牙髓细胞增殖和保护的实验研究[J]. 河北医科大学学报, 2021, 42(7): 765-769.
[4]	王惠敏, 赵增波, 刘晓礼, 张艳宁, 侯敬雅, 杨运田. 生物陶瓷用于年轻恒前牙牙髓切断术的临床效果评价[J]. 河北医科大学学报, 2021, 42(6): 727-729.
[5]	冯一帆, 吕炳建, 平雅坤. 不同麻醉方式下乳磨牙iRoot BP Plus牙髓切断术疗效观察[J]. 河北医科大学学报, 2021, 42(3): 355-357,372.
[6]	朱妮蔓，张诚壹，熊亚芳*. 牙髓血运重建术和氢氧化钙用于年轻恒牙活髓切断的临床观察[J]. 河北医科大学学报, 2020, 41(8): 948-951.
[7]	赵娟，李龙*. 下调MCT1表达抑制宫颈癌Hela细胞增殖并促进其凋亡[J]. 河北医科大学学报, 2020, 41(5): 570-573,578.
[8]	李韵, 毛英, 王海荣, 张海英, 王敬, 李娜. 上调miR-27b表达对人肝癌Huh7细胞系增殖的影响[J]. 河北医科大学学报, 2020, 41(12): 1465-1468.
[9]	徐彦楠, 孟丽, 朱艳, 闫静, 王彦玲, 周晨明. 双氢青蒿素对人胃癌细胞BGC-823细胞增殖、凋亡的影响及机制研究[J]. 河北医科大学学报, 2020, 41(11): 1245-1250.
[10]	庞雅湘1，韩华2，殷玥1，马国华3，牛洪琳4*. 过表达miR-140-5p促进前列腺癌细胞凋亡抑制其增殖[J]. 河北医科大学学报, 2020, 41(1): 7-11.
[11]	左宏玲，闫晓楠，刘素彬，李秀文，王惠兰，徐春琳，杜辉. 雌二醇对卵巢透明细胞癌ES2细胞增殖的影响及机制探讨[J]. 河北医科大学学报, 2019, 40(9): 1029-1033.
[12]	侯佳，陶洪 . 一次性根管术填充纳米羟基磷灰石对牙体牙髓病患者疗效、安全性及咀嚼功能的影响[J]. 河北医科大学学报, 2019, 40(9): 1090-1093.
[13]	聂红峰1，张〓萍2，贺雪霞1，曹〓青1，侯艳青1，孟〓磊1. miR19a3p通过PTEN调节人胃癌细胞增殖水平#br#[J]. 河北医科大学学报, 2019, 40(5): 538-542,546.
[14]	尹〓克1，李天客2*，宋〓艳2，崔子峰2，段玉芹2，陈〓中2. miR214在口腔鳞癌细胞中的表达及生物学分析#br#[J]. 河北医科大学学报, 2019, 40(5): 610-614.
[15]	马建锋1，黎玮2*，李守宾3，张朝华1，邵丽军1，沙泉1. CIAPIN1基因在膀胱癌组织中的表达及其对T24细胞增殖的调节[J]. 河北医科大学学报, 2019, 40(10): 1154-1157,1163.

载脂蛋白D激活Wnt/β-catenin信号通路参与牙髓细胞增殖分化过程

Involvement of apolipoprotein D in the proliferation and differentiation of dental pulp cells by activating Wnt/β-catenin signaling pathway

HTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价