CIAPIN1基因在膀胱癌组织中的表达及其对T24细胞增殖的调节

doi:10.3969/j.issn.1007-3205.2019.10.009

河北医科大学学报

CIAPIN1基因在膀胱癌组织中的表达及其对T24细胞增殖的调节

1.河北省保定市第一医院泌尿外科，河北保定 071000；2.河北医科大学第二医院泌尿外科，河北石家庄 050000；
3.河北省人民医院泌尿外科，河北石家庄 050051

出版日期:2019-10-25 发布日期:2019-10-21
作者简介:马建锋（1977-），男，河北辛集人，河北省保定市第一医院副主任医师，医学硕士，从事泌尿外科疾病诊治研究。

Expression of CIAPIN1 gene in bladder cancer and its regulation on the proliferation of T24 cells#br#

1.Department of Urology, the First Hospital of Baoding, Hebei Province， Baoding 071000, China；
〖JP2〗2.Department of Urology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China；
3.Department of Urology, Hebei General Hospital， Shijiazhuang 050051, China

Online:2019-10-25 Published:2019-10-21

摘要/Abstract

摘要： 〗［摘要］
〖HTH〗目的〖HTSS〗探讨细胞因子诱导的凋亡抑制因子1(cytokine-induced apoptosis inhibitor 1，CIAPIN1)基因在膀胱癌及癌旁组织中的表达及其对膀胱癌T24细胞增殖的影响。
〖HTH〗方法〖HTSS〗收集膀胱癌患者的癌组织及5 cm以外的癌旁组织标本各98例，以癌旁组织作为对照，采用实时定量PCR及Western blot检测组织样本中CIAPIN1 mRNA 和蛋白质的表达情况；利用siRNA敲低膀胱癌T24细胞CIAPIN1基因，MTS法检测细胞的增殖情况。
〖HTH〗结果〖HTSS〗PCR结果显示，膀胱癌组织中CIAPIN1 mRNA表达显著高于癌旁组织（P＜0.05）。组织学分级grade Ⅱ~Ⅲ膀胱癌患者CIAPIN1表达阳性率高于gradeⅠ,TNM分期T2~T4膀胱癌患者CIAPIN1表达阳性率高于Ta~T1(P＜0.05)；不同性别、年龄膀胱癌患者CIAPIN1表达阳性率差异无统计学意义（P＞0.05）。膀胱癌组织中CIAPIN1的蛋白表达水平明显高于癌旁组织（P＜0.05）。MTS结果显示，与对照组相比，随着时间延长敲低CIAPIN1显著抑制T24细胞的OD值，在0 h时，对照组与敲低组细胞的OD值差异无统计学意义（P＞0.05），12 h、24 h、48 h时敲低CIAPIN1基因T24细胞增殖活性明显低于对照组(P＜0.05)。
〖HTH〗结论〖HTSS〗CIAPIN1可能通过调节细胞增殖促进膀胱癌的进展。

关键词: 膀胱肿瘤, 细胞因子诱导的凋亡抑制因子1, 细胞增殖

Abstract: ［Abstract］Objective〖HTSS〗To investigate the expression of cytokine-induced apoptosis inhibitor 1(CIAPIN1) gene in bladder cancer and adjacent tissues and its effect on the proliferation of bladder cancer T24 cells.
〖HTH〗〖WTHZ〗Methods〖HTSS〗Ninety-eight specimens of bladder cancer tissues and adjacent tissues were collected. The expression of CIAPIN1 mRNA and protein in tissue samples was detected by real-time quantitative PCR and Western blot. Knockdown of CIAPIN1 gene in bladder cancer T24 cells by siRNA. Then MTS assay was used to detect cell proliferation.
〖HTH〗〖WTHZ〗Results〖HTSS〗The expression of CIAPIN1 mRNA in bladder cancer tissues was significantly higher than that in adjacent tissues(P＜0.05). The expression of CIAPIN1 is independent of the patient's gender and age. However, the expression of CIAPIN1 was related to histological grade and TNM staging. The expression of CIAPIN1 in grade Ⅱ-Ⅲ bladder cancer patients was higher than that in grade Ⅰ. The expression of CIAPIN1 in TNM stage T2-T4 bladder cancer patients was higher than that in Ta-T1(P＜0.05). Western blot analysis showed that the expression of CIAPIN1 protein in bladder cancer tissues was significantly higher than that in adjacent tissues, but no changed about that of β-actin(P＜0.05). MTS results showed that knockdown of CIAPIN1 significantly inhibited the OD value of T24 cells over time compared to the control group. At 0 h, there was no difference in OD values between the control group and the knockdown group(P＞0.05). The proliferation activity of CIAPIN1 gene T24 cells was significantly lower than that of the control group at 12 h, 24 h, and 48 h(P＜0.05).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗CIAPIN1 may promote the progression of bladder cancer by regulating cell proliferation.

Key words: bladder cancer, CIAPIN1; , proliferation

马建锋1，黎玮2*，李守宾3，张朝华1，邵丽军1，沙泉1. CIAPIN1基因在膀胱癌组织中的表达及其对T24细胞增殖的调节[J]. 河北医科大学学报, doi: 10.3969/j.issn.1007-3205.2019.10.009.

MA Jian-feng1, LI Wei2*, LI Shou-bin3, ZHANG Chao-hua1, SHAO Li-jun1, SHA Quan1. Expression of CIAPIN1 gene in bladder cancer and its regulation on the proliferation of T24 cells#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.1007-3205.2019.10.009.

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CIAPIN1基因在膀胱癌组织中的表达及其对T24细胞增殖的调节

Expression of CIAPIN1 gene in bladder cancer and its regulation on the proliferation of T24 cells#br#

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