LncRNA VPS9D1-AS1在膀胱癌中的表达对临床表现和恶性生物学行为的影响

doi:10.3969/j.issn.1007-3205.2025.02.006

河北医科大学学报 ›› 2025, Vol. 46 ›› Issue (2): 159-165.doi: 10.3969/j.issn.1007-3205.2025.02.006

LncRNA VPS9D1-AS1在膀胱癌中的表达对临床表现和恶性生物学行为的影响

河北医科大学第四医院泌尿外科，河北石家庄 050011

出版日期:2025-02-25 发布日期:2025-02-27
作者简介:刘宗航(1997-)，男，河北石家庄人，河北医科大学第四医院医学硕士研究生，从事泌尿外科疾病诊治研究。
基金资助:
河北省重点研发计划项目(20377706D)

Effect of expression of lncRNA VPS9D1-AS1 in bladder cancer on clinical manifestations and malignant biological behavior of bladder cancer

Department of Urology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China

Online:2025-02-25 Published:2025-02-27

摘要/Abstract

摘要： 目的探究长链非编码RNA（long non-coding RNA,lncRNA）VPS9D1-AS1在膀胱癌中的表达水平对膀胱癌临床表现和恶性生物学行为的影响。
方法收集河北医科大学第四医院泌尿外科收治膀胱癌患者的癌组织和正常膀胱上皮组织样本各69例，应用实时荧光定量多聚核苷酸酶链式反应（quantitative real-time polymerase chain reaction,qPCR）检测VPS9D1-AS1在膀胱癌组织中的表达水平，分析其与性别、年龄、吸烟、高血压病、病理分级、T分期、淋巴结转移、远处转移之间的关联。通过细胞增殖实验、细胞集落形成实验、细胞划痕实验和Transwell侵袭实验探究敲低和过表达VPS9D1-AS1对膀胱癌细胞体外增殖、迁移和侵袭能力的影响。
结果 VPS9D1-AS1在膀胱癌组织中的表达水平显著高于正常膀胱上皮组织（P＜0.01）；VPS9D1-AS1在高级别尿路上皮癌组的表达水平显著高于低级别尿路上皮癌组（P＜0.05）；敲低和过表达VPS9D1-AS1可分别抑制和增强膀胱癌细胞系的增殖、迁移和侵袭能力。
结论 lncRNA VPS9D1-AS1提高了膀胱癌细胞的恶性程度，增强了膀胱癌细胞的恶性生物学行为。

关键词: 膀胱肿瘤, 非编码RNA, 生物学行为

Abstract: Objective To investigate the effect of the expression of long noncoding RNA (lncRNA) VPS9D1-AS1 in bladder cancer on the clinical manifestations and malignant biological behavior of bladder cancer.
Methods In total, 69 samples of cancer tissue and normal bladder epithelium were collected from patients with bladder cancer, who were treated in the Department of Urology, the Fourth Hospital of Hebei Medical University. The expression of VPS9D1-AS1 in bladder cancer tissue was detected by quantitative real-time polymerase chain reaction (qPCR), and its correlation with gender, age, smoking, hypertension, pathological grades, T stages, lymph node metastasis and distant metastasis was analyzed. The impacts of knockdown and overexpression of VPS9D1-AS1 on proliferation, migration and invasion ability of bladder cancer cells in vitro were investigated by cell proliferation assay, colony-forming assay, wound healing assay and Transwell invasion assay.
Results The expression of VPS9D1-AS1 in the bladder cancer tissue was significantly higher than that in the normal bladder epithelium (P＜0.01). The expression of VPS9D1-AS1 in the high-grade urothelial carcinoma group was significantly higher than that in the low-grade urothelial carcinoma group (P＜0.01). Knockdown and overexpression of VPS9D1-AS1 could inhibit and enhance the proliferation, migration and invasion ability of bladder cancer cell lines, respectively.
Conclusion LncRNA VPS9D1-AS1 increases the malignancy of bladder cancer cells and enhances the malignant biological behavior of bladder cancer cells.

Key words: urinary bladder neoplasms, non-codingRNA, biological behavior

刘宗航, 齐盼, 李思杰, 段亚涛, 张爱莉. LncRNA VPS9D1-AS1在膀胱癌中的表达对临床表现和恶性生物学行为的影响 [J]. 河北医科大学学报, 2025, 46(2): 159-165.

LIU Zong-hang, QI Pan, LI Si-jie, DUAN Ya-tao, ZHANG Ai-li. Effect of expression of lncRNA VPS9D1-AS1 in bladder cancer on clinical manifestations and malignant biological behavior of bladder cancer [J]. Journal of Hebei Medical University, 2025, 46(2): 159-165.

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LncRNA VPS9D1-AS1在膀胱癌中的表达对临床表现和恶性生物学行为的影响

Effect of expression of lncRNA VPS9D1-AS1 in bladder cancer on clinical manifestations and malignant biological behavior of bladder cancer

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