河北医科大学学报 ›› 2025, Vol. 46 ›› Issue (4): 444-452.doi: 10.3969/j.issn.1007-3205.2025.04.012

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hsa_circRNA_0073762促进甲状腺乳头状癌细胞侵袭、转移和增殖的机制研究

  

  1. 河北医科大学第一医院普外科,乳腺甲状腺诊疗中心，河北 石家庄 050031

  • 出版日期:2025-04-25 发布日期:2025-04-17
  • 作者简介:贾恒哲（1998-），男，河北隆尧人，河北医科大学第一医院医师，医学硕士，从事普外科疾病诊治研究。

  • 基金资助:
    河北省重点研发计划项目（21372401D）

Mechanism of hsa_circRNA_0073762 in promoting invasion, metastasis, and proliferation of papillary thyroid carcinoma cells

  1. Department of General Surgery/Breast and Thyroid Diagnosis and Treatment Center, the First Hospital of 
    Hebei Medical University, Shijiazhuang 050031, China

  • Online:2025-04-25 Published:2025-04-17

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摘要： 目的 探讨hsa_circ_0073762在甲状腺乳头状癌（papillary thyroid carcinoma,PTC）中的调节作用，揭示其具体作用机制。
方法 采用高通量测序鉴定PTC与正常细胞差异表达环状RNA（circRNA），逆转录-定量聚合酶链式反应(reverse transcription-quantitative polymerase chain reaction，RT-qPCR）验证细胞系及PTC与癌旁组织差异表达，核酸电泳、sanger测序、RNA酶消化实验验证其成环性及稳定性，通过细胞计数试剂盒8（cell counting kit-8,CCK-8）、划痕愈合、克隆形成和迁移实验，分析hsa_circ_0073762对PTC细胞增殖、迁移和侵袭的影响，双荧光素酶报告实验验证hsa_circ_0073762、miR-548c-3p、ENAH基因的相互作用。
结果 RT-qPCR验证测序结果显示，hsa_circ_0073762在PTC细胞中显著上调(P＜0.001)，且在癌组织中显著上调（P＜0.05）。敲低hsa_circ_0073762可显著抑制PTC细胞的侵袭、转移和增殖能力。生物信息学预测和双荧光素酶报告实验表明hsa_circ_0073762、miR-548c-3p、ENAH构成ceRNA网络（P＜0.05）。
结论 hsa_circ_0073762对PTC侵袭、转移和增殖进程存在影响，可能成为PTC的新型诊断生物标志物和潜在治疗靶点。


关键词: 甲状腺癌, 乳头状, RNA, 环状, 肿瘤浸润

Abstract: Objective To investigate the regulatory role of hsa_circ_0073762 in papillary thyroid carcinoma (PTC) and to explore its underlying mechanisms. 
 Methods High-throughput sequencing was used to identify differentially expressed circular RNAs (circRNAs) in thyroid cancer and normal cells. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to validate the differential expression between cell lines, PTC, and adjacent tissues. Nucleic acid electrophoresis, sanger sequencing, and RNA enzyme digestion experiments were conducted to verify the circularity and stability of hsa_circ_0073762. Cell counting kit-8 (CCK-8), wound healing, clone formation, and migration assays were used to analyze the effects of hsa_circ_0073762 on PTC cell proliferation, migration, and invasion. A dual-luciferase reporter assay was used to validate the interaction between hsa_circ_0073762, miR-548c-3p, and the ENAH gene. 
 Results RT-qPCR validation of sequencing results showed that hsa_circ_0073762 was significantly upregulated in PTC cells (P＜0.001) and in cancer tissues (P＜0.05). Knockdown of hsa_circ_0073762 significantly inhibited the invasion, migration, and proliferation of PTC cells. Bioinformatics prediction and dual-luciferase reporter assays indicated that hsa_circ_0073762, miR-548c-3p, and ENAH formed a ceRNA network (P＜0.05). 
 Conclusion hsa_circ_0073762 has effects on the invasion, migration, and proliferation processes of PTC and may serve as a novel diagnostic biomarker and potential therapeutic target for PTC. 


Key words: thyroid cancer, papillary, RNA, circular, neoplasm invasiveness 

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