河北医科大学学报

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载脂蛋白C1表达与血管内膜增生关系的初步研究

  

  1. 河北医科大学基础医学院生物化学与分子生物学教研室，河北 石家庄 050017
  • 出版日期:2017-02-25 发布日期:2017-03-10
  • 作者简介:陈含冰（1994-），女，河北衡水人，河北医科大学基础医学院2012级临床医学专业本科生。
  • 基金资助:
    国家自然科学基金课题（91439114，31271222）

The preliminary study on correlation between APOC1 and vascular neointimal formation#br#

  1. Department of Biochemistry and Molecular Biology, College of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China
  • Online:2017-02-25 Published:2017-03-10

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摘要： 摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗探讨载脂蛋白（apolipoprotein, APO）C1表达与血管重塑的关系。
〖HTH〗方法〖HTSS〗〖KG*2〗利用颈总动脉结扎术制备血管内膜增生模型，采用HE染色检测内膜增生情况，采用Western blot检测血管组织APOC1与SM22α蛋白表达水平，采用组织免疫荧光检测APOC1在血管壁的分布。
〖HTH〗结果〖HTSS〗〖KG*2〗随着结扎时间的延长，新生内膜明显增厚，小鼠颈总动脉结扎术后不同时间点，血管新生内膜中SM22α表达量逐渐降低，APOC1表达量逐渐升高，两者变化趋势相反。免疫荧光染色亦显示，在增厚的内膜区，APOC1荧光强度增强，与内膜厚度呈正相关。
〖HTH〗结论〖HTSS〗〖KG*2〗APOC1表达上调可能参与平滑肌细胞表型转化和血管重塑过程。

关键词: 血管内膜, 载脂蛋白C类, 肌, 平滑

Abstract: ［Abstract］ Objective〖HTSS〗〓To discuss the correlation of apolipoprotein (APO) C1 expression and neointimal formation.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The intimal hyperplasia model of the mice was prepared by the common carotid artery ligation. HE staining was used to test the neointima. Western blot was used to detect the protein expression of APOC1 and SM22α, Immunofluorescence was used to detect the protein expression and distribution of APOC1.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓With the extension of time of ligation,the intimal hyperplasia was observed in the carotid arteries of mice after the ligation. At different time points of the common carotid artery ligation. the expression of SM22α was decreased in neointimal region. The expression of APOC1 was gradually increased, which was contrary to that of SM22. Immunofluorescence staining also showed that the fluorescence intensity of APOC1 was enhanced in the area of thickened intima, and it is positively correlated with the thickness of intima.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The expression of APOC1 may be involved in vascular smooth muscle cell switching and vascular remodeling.

Key words: tunica intima, apolipoproteins C, muscle, smooth

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