miR-205通过靶向调控caspase-3表达调节神经胶质瘤的生物学行为

doi:10.3969/j.issn.10073205.2017.09.008

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗探讨微小RNA205、天冬氨酸特异性半胱氨酸蛋白酶3(cyserinly aspartatespecific protease,caspase3)表达水平对神经胶质瘤生物学行为的影响及其可能的作用机制。
〖HTH〗方法〖HTSS〗〖KG*2〗采用实时荧光定量PCR检测30例神经胶质瘤组织及正常脑组织中miR205表达水平；采用脂质体转染技术分别将miR205 模拟物（miR205 mimics）、对照（control mimics）、miR205抑制物（miR205inhibitor）导入至U251细胞中，以Transwell实验观察细胞侵袭能力；采用生物信息学及荧光蛋白报告基因实验验证miR205对caspase3的靶向调控作用；采用RNA干扰技术研究U251细胞中caspase3蛋白表达水平及细胞克隆数量变化。
〖HTH〗结果〖HTSS〗〖KG*2〗神经胶质瘤组织miR205表达值低于正常脑组织(P＜005),30神经胶质瘤组织中28例miR205下调2倍以上。将miR205模拟物或miR205抑制剂转染入U251细胞中发现，在miR205的表达水平上，miR205模拟物显著高于对照，miR205抑制物低于对照，差异有统计学意义(P＜005)。miR205模拟物转染U251细胞后caspase3蛋白表达高于对照转染后细胞（P＜005）。miR205模拟物+pcDNA3Wt共转染组荧光值显著高于对照及miR205模拟物+pcDNA3Mut（P＜005）。转染miR205模拟物组，其细胞克隆形成明显少于对照组及空白组，而转染miR205 inhibitor后，其细胞克隆形成则多于空白组（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗多数神经胶质瘤存在miR205表达下调现象，而miR205可能通过靶向调控caspase3影响胶质瘤细胞的侵袭、增殖、克隆形成能力，这将为进一步认识及治疗神经胶质瘤提供有力依据。

关键词: 神经胶质瘤, 微RNAs, 半胱氨酸天冬氨酸蛋白酶3

Abstract: ［Abstract］ Objective〖HTSS〗〓To investigate the effect of microRNA205 and aspartatespecific caspase3 expression on the biological behavior of glioma and its possible mechanism.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The expression of miR205 in 30 cases of glioma tissue and normal brain tissue was detected by realtime fluorescence quantitative PCR. The miR205 mimics, control mimics, miR205 inhibitor(miR205inhibitor) was introduced into U251 cells and the invasive ability of the cells was observed by Transwell assay. The effect of miR205 on caspase3 was tested by bioinformatics and fluorescent protein reporter assay. The expression level of caspase3 protein and the number of cell clones in U251 cells were studied by RNA interference technique.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The expression of miR205 in glioma tissue was lower than that in normal brain tissue(P＜005), and 28 cases of glioma tissue were 28 times lower than that of miR205. The miR205 mimetic or miR205 inhibitor was transfected into U251 cells and found that miR205 mimics were significantly higher than those of control at miR205 expression levels, and that of miR205 was lower than that of control. The difference was statistically significant(P＜005). The expression of caspase3 protein in U251 cells transfected with miR205 mimics was higher than that in control transfected cells(P＜005). The fluorescence value of miR205 mimics+pcDNA3Wt cotransfection group was significantly higher than that of control and miR205 mimics+pcDNA3Mut(P＜005). The miR205 mimetic group was transfected into miR205 mimetic group, and its cell clone formation was significantly less than that of the control group and the blank group. After transfection of miR205 inhibitor, the cell clone formation was more than that in the blank group(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Most of the gliomas have miR205 expression downregulation, and miR205 may influence the invasion, proliferation and clonal ability of glioma cells by targeting caspase3, which will provide a powerful basis for further understanding and treatment of glioma.

Key words: glioma, microRNAs, caspase3

王〓潇. miR-205通过靶向调控caspase-3表达调节神经胶质瘤的生物学行为[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2017.09.008.

WANG Xiao. Effect of miR-205 on the biological behavior of glioma via targeting caspase-3#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2017.09.008.

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miR-205通过靶向调控caspase-3表达调节神经胶质瘤的生物学行为

Effect of miR-205 on the biological behavior of glioma via targeting caspase-3#br#

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