Wip1在卵巢浆液性囊腺癌组织中的表达及意义

doi:10.3969/j.issn.10073205.2018.08.015

河北医科大学学报

Wip1在卵巢浆液性囊腺癌组织中的表达及意义

1.河北医科大学第一医院生殖科，河北石家庄 050031；2.河北医科大学第一医院妇产科，河北石家庄 050031;
3.河北医科大学第四医院妇产科，河北石家庄 050011

出版日期:2018-08-25 发布日期:2018-07-25
作者简介:冯艳萍(1976-)，女，河北邯郸人，河北医科大学第一医院副主任医师，医学博士，从事妇科内分泌及妇科肿瘤诊治研究。
基金资助:
河北医学科学研究重点课题（20150204）

Expression of Wip1 in ovarian serous cystadenocarcinoma tissues and its significance

1.Department of Reproductive Medicine, the First Hospital of Hebei Medical University, Shijiazhuang
050031, China; 2.Department of Obstetrics and Gynecology, the First Hospital of Hebei
Medical University, Shijiazhuang 050031, China; 3.Department of Obstetrics and
Gynecology, the Fourth Hospital of Hebei Medical University,
Shijiazhuang 050011, China

Online:2018-08-25 Published:2018-07-25

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗观察野生型p53诱导的蛋白磷酸酶1（Wildtype p53induced phosphatase 1,Wip1）在卵巢浆液性囊腺癌组织中的表达并探讨Wip1与卵巢浆液性囊腺癌临床病理特征的关系。
〖HTH〗方法〖HTSS〗〖KG*2〗采用免疫组织化学、实时荧光定量反转录-聚合酶链反应（realtime fluorescent quantitative reverse transcriptionpolymerase chain reaction,RTPCR）及Western blot方法检测卵巢浆液性囊腺癌50例、交界性卵巢浆液性囊腺瘤20例、正常卵巢组织10例中Wip1表达阳性率、Wip1 mRNA和Wip1蛋白的表达水平。
〖HTH〗结果〖HTSS〗〖KG*2〗卵巢浆液性囊腺癌组织和交界性卵巢浆液性囊腺瘤中Wip1的表达阳性率明显高于正常卵巢组织，差异有统计学意义（P＜005）；卵巢浆液性囊腺癌与交界性卵巢浆液性囊腺瘤组织中Wip1表达阳性率差异无统计学意义(P＞005)；卵巢浆液性囊腺癌组和交界性卵巢浆液性囊腺瘤组中Wip1 mRNA的表达明显高于正常卵巢组织，差异有统计学意义（P＜005）；卵巢浆液性囊腺癌组和交界性卵巢浆液性囊腺瘤组中Wip1蛋白的表达明显高于正常卵巢组织，差异有统计学意义（P＜005）；卵巢浆液性囊腺癌组中病理分期Ⅲ+Ⅳ期患者较Ⅰ+Ⅱ期患者Wip1表达阳性率高，差异有统计学意义(P＜005)；有淋巴结转移者较无淋巴结转移者Wip1表达阳性率高，差异有统计学意义(P＜005)；不同组织分级患者Wip1表达阳性率差异无统计学意义(P＞005)。
〖HTH〗结论〖HTSS〗〖KG*2〗Wip1在卵巢浆液性囊腺癌组织中高表达，与淋巴结转移、临床分期、组织学分级密切相关。Wip1参与了卵巢浆液性囊腺癌的发生发展过程。

关键词: 卵巢肿瘤, 蛋白磷酸酶1, 病理学, 临床

Abstract: ［Abstract］ Objective〖HTSS〗〓To explore expression of wildtype p53induced phosphatase 1(Wip1) in ovarian serous cystadenocarcinoma tissues and its relationship with clinicopathological features.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The positive expression of Wip1 as well as the expression of Wip1 mRNA and Wip1 protein in ovarian serous cystadenocarcinoma tissues(n=50),ovarian serous cystadenoma tissues(n=20) and normal ovarian tissues(n=10) were detected by immunohistochemistry, realtime fluorescent quantitative reverse transcriptionpolymerase chain reaction(RTPCR) and Westernblot.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓 The expression of Wip1 was significantly higher in ovarian serous cystadenocarcinoma and borderline ovarian serous cystadenoma tissues than in normal ovarian tissues (P＜005). There was no significant difference in positive expression of Wip1 between ovarian serous cystadenocarcinoma tissues and borderline ovarian serous cystadenoma tissues(P＞0.05). Wip1 mRNA expression in borderline ovarian serous cystadenoma and ovarian serous cystadenocarcinoma tissues were highly increased, as compared with normal ovarian tissues,and the difference was significant (P＜005). The expressions of Wip1 protein were remarkably higher in ovarian serous cystadenocarcinoma and ovarian serous cystadenocarcinoma tissues than in normal ovarian tissues, and the difference was statistically significant (P＜005). The positive expression of Wip1 in stage Ⅲ and Ⅳ of ovarian serous cystadenocarcinoma tissues was significantly higher than that in stageⅠandⅡ(P＜005). The positive expression of Wip1 was notably higher in groups with lymph node metastasis, as compared with groups without lymph node metastasis(P＜005). There was no significant difference in the positive expression of Wip1 in carcinomas of different histological grades(P＞005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Wip1 is highly expressed in ovarian serous cystadenocarcinoma,which is closely associated with lymph node metastasis, clinical staging and histological grade. It is suggested that Wip1 is involved in the development and progression of ovarian serous cystadenocarcinoma.

Key words: ovarian neoplasms, phosphatase 1, pathology, clinical

冯艳萍1，郭〓伟1，刘〓晶2，张红真2*，程建新3. Wip1在卵巢浆液性囊腺癌组织中的表达及意义[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2018.08.015.

FENG Yanping1, GUO Wei1, LIU Jing2, ZHANG Hongzhen2*, CHENG Jianxin3. Expression of Wip1 in ovarian serous cystadenocarcinoma tissues and its significance[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2018.08.015.

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Wip1在卵巢浆液性囊腺癌组织中的表达及意义

Expression of Wip1 in ovarian serous cystadenocarcinoma tissues and its significance

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