河北医科大学学报

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过表达miR-140-5p促进前列腺癌细胞凋亡抑制其增殖

  

  1. 1.河北医科大学临床学院实验中心，河北 石家庄 050031；2.河北省人民医院妇一科，河北 石家庄 050051；
    3.河北医科大学教学实验中心显微镜室，河北 石家庄 050017；4.河北医科大学第三医院肾内科，河北 石家庄 050051
  • 出版日期:2020-01-25 发布日期:2020-02-25
  • 作者简介:庞雅湘（1979-），女，湖南南县人，河北医科大学临床学院实验师，医学硕士，从事实验中心管理研究。
  • 基金资助:
    国家自然科学基金青年科学基金项目（81600576）

Overexpression of miR-140-5p promotes apoptosis and inhibits proliferation of prostate cancer cells#br#

  1. 1.Department of Experimental Centre, the School of Clinical, Hebei Medical University, Shijiazhuang
    050031, China; 2.The First Department of Obstetrics and Gynecology, Hebei General Hospital,
    Shijiazhuang 050051, China; 3.Department of Microscopy Room Teaching Experiment
    Center of Hebei Medical University, Shijiazhuang 050017, China; 4.Department of
    Nephrology, the Third Hospital of Hebei Medical University,
    Shijiazhuang 050051, China
  • Online:2020-01-25 Published:2020-02-25

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摘要： ［摘要］
目的  研究miR-140-5p在前列腺癌细胞中的表达情况，探讨 miR-140-5p在前列腺癌细胞中的生物学功能。
方法  RT-qPCR方法检测前列腺癌细胞株PC3细胞及DU145细胞中miR-140-5p表达；PC3细胞中转染miR-140-5p模拟物或抑制物，CCK-8及AnnexinV/PI双染流式细胞学检测细胞活力及细胞凋亡。TCGA数据库分析高表达miR-140-5p与前列腺癌患者总生存率的关系。
结果  miR-140-5p在DU145及PC3细胞中的表达明显低于RWPE-1细胞，miR-140-5p在PC3细胞中的表达明显低于DU145细胞（P＜0.05）。转染miR-140-5p模拟物的 PC3细胞中miR-140-5p相对表达量明显高于阴性对照组（P＜0.05）。miR-140-5p的模拟物转染PC3细胞48 h，过表达组细胞增殖率低于阴性对照组，凋亡率高于阴性对照组（P＜0.05）。转染miR-140-5p抑制物的PC3细胞中miR-140-5p相对表达量明显低于阴性对照组（P＜0.05）。敲低miR-140-5p表达组细胞增殖率明显高于阴性对照组，凋亡率明显低于阴性对照组（P＜0.05）。低表达miR-140-5p组患者总生存率低于高表达miR-140-5p组患者（P＜0.05）。
结论  miR-140-5p在前列腺癌细胞株低表达，低表达miR-140-5p前列腺癌患者预后不良；过表达miR-140-5p可促进前列腺癌细胞凋亡，抑制其增殖。

关键词: 前列腺肿瘤, 微小RNA, 细胞凋亡, 细胞增殖

Abstract: ［Abstract］Objective〖HTSS〗To investigate the expression of miR-140-5p in prostate cancer cells and explore the biological functions of miR-140-5p in prostate cancer progression.
Methods〖HTSS〗RT-qPCR was performed to identify the expression of miR-140-5p in PC3 and DU145 cell lines. CCK8 assay and AnnexinV-FITC/PI stain were used to exam cell proliferation cell apoptosis. TCGA database was used to analyze the relationship between miR-140-5p expression level and survival of prostate cancer patient.
Results〖HTSS〗miR-140-5p was downregulated in PC3 and DU145 cells.Transfection of miR-140-5p mimic to PC3 cells significantly increased miR-140-5p level in PC3 cells. Overexpression of miR-140-5p in PC3 cells increased the cell apoptosis and decreased cell viability. Transfection of miR-140-5p inhibitor to PC3 cells significantly decreased miR-140-5p level in PC3 cells. Knockdown of miR-140-5p in PC3 cells decreased cell apoptosis and increased cell viability. Lower miR-140-5p level of prostate cancer patients had a poor overall survival analyzing from TCGA database.
Conclusion〖HTSS〗miR-140-5p is downregulated in prostate cell lines and correlates with poor survival of prostate cancer. Overexpression of miR-140-5p in PC3 cells promotes cell apoptosis and inhibits cell growth.

Key words: prostatic neoplasms, microRNA, apoptosis, cell proliferation

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