河北医科大学学报 ›› 2024, Vol. 45 ›› Issue (4): 418-423.doi: 10.3969/j.issn.1007-3205.2024.04.009

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Nd:YAG激光联合氟保护漆预防乳切牙牙本质龋再脱矿的体外研究

  

  1. 河北医科大学口腔医学院，口腔医院儿童口腔科，河北省口腔医学重点实验室，河北省口腔疾病临床医学研究中心,河北 石家庄 050017

  • 出版日期:2024-04-25 发布日期:2024-04-22
  • 作者简介:韩宁（1995-），女，河北邯郸人，河北医科大学口腔医院医学硕士研究生，从事儿童口腔疾病诊治研究。
  • 基金资助:
    河北省政府资助临床医学优秀人才培养和基础课题研究项目(MXZB00135)

Effect of Nd:YAG laser combined with fluorine varnish on preventing redemineralization of dentine caries in deciduous incisors in vitro

  1. Department of Pediatric Dentistry, Hebei Key Laboratory of Stomatology, Hebei Clinical 
    Research Center for Oral Diseases, School and Hospital of Stomatology, 
    Hebei Medical University, Shijiazhuang 050017, China

  • Online:2024-04-25 Published:2024-04-22

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摘要： 目的 使用激光扫描共聚焦显微镜评估Nd:YAG激光联合氟保护漆预防乳切牙牙本质龋再脱矿的效果。
方法 收集30颗乳切牙,按不同治疗方式随机分为A、B、C 3组(n=10),每颗牙分成近、远中两部分，分别作为试验组和对照组，进行自身对照研究，A组为Nd:YAG激光组(A1组为试验组,A2组为对照组),B组为氟保护漆组(B1组为试验组,B2组为对照组),C组为Nd:YAG激光联合氟保护漆组(C1组为试验组,C2组为对照组)。人工制造牙本质浅龋模型，经过不同方式处理后再次脱矿,使用含荧光素钠的乙醇溶液染色,并制备成磨片,用激光扫描共聚焦显微镜观察样本牙纵剖面的脱矿深度。
结果 3个组的试验组（A1组、B1组、C1组）脱矿带宽度均小于对照组（A2组、B2组、C2组），差异有统计学意义(P＜0.01)；3个组的对照组（A2组、B2组、C2组）脱矿深度差异无统计学意义(P＞0.05)；3个组的试验组（A1组、B1组、C1组）脱矿带宽度差异有统计学意义(P＜0.01)，C1组脱矿带宽度明显小于A1组、B1组，差异有统计学意义(P＜0.01)。
结论 Nd:YAG激光和氟保护漆单独应用,均能达到减缓乳切牙牙本质龋再脱矿的目的,两者联合应用效果更佳。


关键词: 龋齿, 牙, 乳, Nd:YAG激光, 氟保护漆

Abstract: Objective To evaluate the combined effect of Nd:YAG laser treatment and fluorine varnish on the prevention of redemineralization of dentine caries in deciduous incisors using confocal laser scanning microscope (CLSM). 
Methods Thirty deciduous incisors were collected and randomly divided into three groups, group A (n=10), group B (n=10), and group C (n=10), according to the different treatment methods. Each tooth was divided into mesial and distal parts, which were used as the experimental group and the control group, respectively, in the self-controlled study. Group A received only Nd:YAG laser treatment, including group A1 as the experimental group and group A2 as the control group. Group B was treated with fluorine varnish only, including group B1 as the experimental group and group B2 as the control group, while group C received the combined Nd:YAG laser and fluorine varnish treatment, including group C1 as the experimental group and group C2 as the control group. The artificial superficial dentine caries models were redemineralized after treatment, stained with a solution of fluorescein sodium in ethanol, and ground using grinding plates. The depth of demineralization in longitudinal sections of the teeth was evaluated by CLSM. 
Results The depth of demineralization differed significantly between groups A1 and A2, between groups B1 and B2, and between groups C1 and C2 (P＜0.01). No significant differences in the demineralization depths were found among groups A2, B2, and C2 (P＞0.05). The depth of demineralization differed significantly between groups A1 and C1, and between groups B1 and C1 (P＜0.01), which was smaller in group C1 than in groups A1 and B1 (P＜0.01). 
Conclusion While individual administration of the Nd:YAG laser treatment and fluorine varnish are effective in alleviating the redemineralization of dentine caries in deciduous incisors, the combined treatment is significantly more effective. 


Key words: dental caries, tooth, deciduous, Nd:YAG lasers, fluorine varnish

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