关键词: 卵巢肿瘤, 微RNAS, 血管内皮生长因子类

Abstract: Objective To detect the expression levels of miRNA-107(miR-107)in ovarian cancer tissues and its impact on vascular endothelial growth factor A(VEGFA). 
Methods A total of 56 patients with ovarian cancer were enrolled as case group,and 60 patients who underwent total hysterectomy and bilateral adnexectomy for uterine prolapse, cervical cancer or uterine leiomyoma during the same period were selected as control group. Real-time quantitative PCR(RT-qPCR) was carried out to detect the expression levels of miR-107 and VEGFA mRNA, and the correlation between the expression levels of miR-107 and VEGFA mRNA in the ovarian cancer tissues was analyzed. ROC curves were applied to analyze the diagnostic value of miR-107 levels in ovarian cancer tissues for ovarian cancer. 
Results RT-qPCR showed that the expression levels of miR-107 were significantly lower in ovarian cancer tissues than in the control group, and relative expression levels of VEGFA mRNA were significantly higher in ovarian cancer tissues than in the control group(P＜0.05). Moreover, the expression levels of miR-107 and VEGFA mRNA were significantly correlated with FIGO stages and lymph node metastasis of patients with ovarian cancer(P＜0.05). Pearson correlation analysis revealed that the expression levels of miR-107 was significantly negatively correlated with the expression levels of VEGFA mRNA in the ovarian cancer tissues(r=-0.498， P＜0.001). The area under the ROC curve of miR-107 expression level in predicting ovarian cancer was 0.936(P＜0.001), the optimal cut-off value for ovarian cancer was 1.639, and the sensitivity and specificity were 94.6% and 76.7%respectively. 
Conclusion The decreased expression of miR-107 may promote the invasion and metastasis of ovarian cancer cells by targeting up-regulation of VEGFA expression, thereby playing an important role in the development of ovarian cancer. 

Key words: ovarian neoplasms, MicroRNAs, vascular endothelial growth factors