河北医科大学学报 ›› 2021, Vol. 42 ›› Issue (9): 998-1002.doi: 10.3969/j.issn.1007-3205.2021.09.002

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不同冰冻条件对肌性组织标本冻存效果的影响

  

  1. 1.河北医科大学研究生学院，河北 石家庄 050017；2.河北医科大学生物化学与分子生物学教研室，河北 石家庄 050017
  • 出版日期:2021-09-25 发布日期:2021-09-28
  • 作者简介:孙红星（1996-），男，河北唐山人，河北医科大学基础医学院医学硕士研究生，从事血管生物学研究。
  • 基金资助:
    国家自然科学基金课题（91849102）；河北省自然科学基金项目（H2019206028）

Effect of different freezing conditions on the freezing preservation of muscle tissue specimens

  1. 1.Graduate School of Hebei Medical University, Shijiazhuang 050017, China; 2.Department of 
    Biochemistry and Molecular Biology, Hebei Medical University, Shijiazhuang 050017, China
  • Online:2021-09-25 Published:2021-09-28

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摘要： 目的 比较不同肌性组织在三种冰冻条件下的切片效果，筛选出不同肌性组织的最优冰冻方法。
方法 采用骨骼肌（腓肠肌）、心肌、平滑肌（胸主动脉）；三种冰冻条件分别为液氮浸入法、液氮漂浮法、直接-80 ℃法。通过取材，冰冻，切片和苏木精-伊红（hematoxylin-eosin,HE）染色，最后在镜下观察组织结构形态。
结果 骨骼肌冰冻的最优条件是液氮浸入法，心肌冰冻的最优条件是液氮漂浮法，血管平滑肌冰冻的最优条件是液氮浸入法或液氮漂浮法。
结论 不同肌性组织最适宜的冻存条件存有差异。


关键词: 肌, 冰冻切片, 冻存方法, 条件优化

Abstract: Objective To select the optimal freezing method for different muscular tissues by comparing the effect of different muscular tissue sections under three freezing conditions. 
Methods The tissues used in this experiment included skeletal muscle(gastrocnemius), myocardium, and vascular smooth muscle(thoracic aorta). The three freezing conditions were liquid nitrogen immersion, floating on liquid nitrogen, and direct -80 ℃ methods.After sampling, freezing, sectioning and hematoxylin-eosin(HE) staining, the tissue structure morphology was observed under a microscope. 
Results The optimal freezing condition for the skeletal muscle, myocardium and vascular smooth muscle was liquid nitrogen immersion, floating on liquid nitrogen, and liquid nitrogen immersion or floating on liquid nitrogen, respectively. 
Conclusion There are differences in the optimal freezing conditions for different muscular tissues.


Key words: muscles, frozen section, freezing method, condition optimization

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