Abstract: ［Abstract］Objective〖HTSS〗To investigate the significance of growth hormone(GH) in patients with endometriosis(EMs) and its effect on apoptosis of ovarian luteinized granulosa cells.
〖HTH〗〖WTHZ〗Methods〖HTSS〗A total of forty infertility patients with endometriosis who underwent in vitro fertilization-embryo transfer(IVF-ET) (EMs group) and forty infertility patients with simple fallopian tube factors who underwent IVF-ET(control group) were screened and enrolled in the study. The laboratory indicators and pregnancy outcomes of the two groups were compared. The serum GH levels and follicular fluid GH levels of the two groups on the day of oocyte retrieval were measured by radioimmunoassay. The expression of Bcl-2 protein and Bax protein in granulosa cells extracted by density gradient method were detected by immunocytochemistry after cell adherence in vitro culture. The granulosa cells of EMs patients were divided into blank group and GH group according to the intervention method after adherence.The expression levels of Bcl-2 protein and Bax protein were detected by immunocytochemistry.
〖HTH〗〖WTHZ〗Results〖HTSS〗The GH levels of serum and follicular fluid in EMs group were significantly lower than those in control group. The expression of Bcl-2 protein in granulosa cells of the EMs group was significantly lower than that of the control group .The expression level of Bax protein was higher than that of the control group, with statistically significant differences(P＜0.05). The expression of Bcl-2 protein increased in GH group compared with blank group, and the expression of Bax protein decreased in GH group compared with blank group. These differences were statistically significant(P＜0.05).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗Low growth hormone levels in patients with endometriosis may cause an increase in granulosa cell apoptosis, which affects oocyte quality and embryonic developmental potential. A certain concentration of growth hormone may improve the granulosa cell apoptosis state in patients with endometriosis.

Key words: endometriosis, growth hormone, in vitro fertilization and embryo transfer