Abstract: ［Abstract］Objective To make targeted infection control strategies by analyzing pathogenic bacteria and risk factors of laparoscopic incision infection in gastrointestinal surgery.
Methods The infection of incision in laparoscopic operation of gastrointestinal surgery was statistically analyzed.
Results A total of 21 cases of 867 patients developed wound infection, the infection rate was 2.42%. The highest infection rate was appendectomy. Pathogen analysis: 7 strains of gram-positive bacteria(33.33%), 13 strains of gram-negative bacteria(61.90%), and 1 strain of fungus(4.76%). Patients with age >60 years old, body mass index（BMI）>25, diabetes mellitus, operation time>90 minutes, sample passing at incision, no use of antibiotics, anesthesia score of grade Ⅲ-Ⅳ, abdominal infection, no incision protection, lower digestive tract at operation site and hospitalization time >8 days had higher incidence of infection. Logistic regression analysis showed that age＞60 years, BMI＞25, and those with diabetes were risk factors for laparoscopic incision infection （P＜0.05）.
Conclusion Special attention should be paid to the prevention of incision infection in laparoscopic surgery for the elderly, obese and diabetic patients.

Key words: infection of laparoscopy incision, pathogenic bacteria, risk factors