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    25 March 2016, Volume 37 Issue 3
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    Thepathologicalparametersandprognosisofbreastcancerpatientswithtype2diabetesmellitus
    LIU Gui-hong1, ZHOU Wei-hua1, ZHANG Yu-na1, LIU Yue-qin1, ZHU Tie-nian2*
    2016, 37(3):  249-251,255.  doi:10.3969/j.issn.10073205.2016.03.001
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    [
    Abstract ] Objective Toinvestigatetherelationshipoftype2diabetesmellituswiththe
    pathologicalparametersandprognosisofbreastcancer.Methods Eightybreastcancerpatients
    withtype2diabetesmellituswereenrolledintothisstudy.Onehundredandsixtybreastcancer
    patientswithouttype2diabetesmellitusinthesametimewereselectedascontrolgroup.The
    generalcharacteristicsandpathologicalparameterswerecompared.Results Theagesandblood
    glucosevaluesinbreastcancerpatientswithtype2diabetesmellitusgroupweresignificantly
    higherthanthoseincontrolgroup , andsoisfortheratioofpostmenopausalpatients.Allthe
    differenceswerestatisticallysignificant.Butthedifferencesofboydmassindex , ageatmenarche
    andparitybetweenthetwogroupswerenotstatisticallysignificant.Therewasnosignificant
    differenceinthedistributionofclinicalstage , tumorsize , lymphnode metastasis , estrogen
    receptor , progesteronereceptor , humanepidermalgrowthfactorreceptor-2and Ki-67status
    betweengroupofbreastcancerpatientswithtype2diabetesmellitusandnon-diabeticgroup.The
    distributionofhistologicalgradeandmoleculartypingbetweengroupofbreastcancerpatients withtype2diabetes mellitusandnon-diabeticgroup weresignificantlydifferent.Thehigher
    histologicalgradeand moreBasal-liketypewerefoundinbreastcancerpatientswithtype2
    diabetes mellitus than in non-diabetic group.The difference was statistically significant.
    Conclusion Breastcancerwithtype2diabetesismorecommoninpatientswitholderageand
    postmenopause.Eldertype2diabetespatientsespeciallyforpostmenopausalshouldberegularly
    examinedbybreastultrasoundandmammography.ThehigherhistologicalgradeandmoreBasal-
    liketypewerefoundingroupofbreastcancerpatientswithtype2diabetesmellitusthaninnon-
    diabeticgroup.Type2diabetesmellitusistheriskfactorforbreastcancerpatientswithpoor
    prognosis.
    Effectofadriamycinonmyocardialfibrosisandpossiblemechanismsinrats
    BAOYun-feng1, WANGJing-jing 2,ZHUGE Ming-ning3,ZHANG Hong-zhen2*
    2016, 37(3):  252-255.  doi:10.3969/j.issn.10073205.2016.03.002
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    [
    Abstract ] Objective Todeterminetheeffectofadriamycinon myocardialfibrosisand
    possible mechanismsin rats.Methods A rat myocardialfibrotic model wasinduced by
    intravenousinjectionofadriamycin ( 3 mg /
    kg
    , onceaweek ) forsix weeks.Duringthetest ,
    generalconditionandbodyweightwereobserved.Attheseventhweek , hemodynamicparameters
    andhydroxyprolineconcentrationaswellasmorphologicaltransformationofhearttissueswere
    detected.Results Comparedtothoseincontrolgroup , hydroxyprolinecontentwasmarkedly
    increasedinadriamycininducedrats.Histopathologicalexaminationfurtherconfirmedthesame
    alteration.Moreover , hemodynamicsshowedasignificantdeclineinleft-ventricularsystolic
    pressure , maximumandminimumrateofdevelopedleftventricularpressure ,
    accompaniedbyan
    incrementofleftventricularend-diastolicpressure ( P <0.05 ) .Conclusion Thesefindings
    suggestedthatadriamycincouldinducemyocardialfibrosisandinhibitcardiacfunction.
    ExpressionsofVEGF , HIF-1αandCOX-2innon-small-celllungcancer
    SHI Guo-yi1, LIU Yu-ling2, WANG Min1
    2016, 37(3):  256-259.  doi:10.3969/j.issn.10073205.2016.03.003
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    [
    Abstract ] Objective Tostudytheexpressionsandclinicopathologicalrelationshipofvascular
    endothelialgrowthfactor ( VEGF ), hypoxiainduciblefactor-1α ( HIF-1α ) andcyclooxygenase-2 ( COX-
    2 ) intissuesofnon-small-celllungcancer ( NSCLC ) .Methods Eighty-ninesamplesofnon-small-
    celllungcancerand17casesofnormallungtissue wereinvolvedinthisstudy , andthe
    expressionsofVEGF , HIF-1αandCOX-2 weredetectedusingimmunohistochemistry ( IHC ) .
    Results ThepositiveratesofVEGF , HIF-1αandCOX-2expressionswere71.9% , 47.2% ,
    57.3% in NSCLC , whichissignificantlyhigherthanthoseinthecontrolgrouprespectively
    (
    29.4% , P =0.001 ; 17.6% , P =0.024 ; 23.5% , P =0.011 ) .Notably , theexpressionsof
    VEGF , HIF-1αandCOX-2 werehigherincaseswithlymphatic metastasisthaninnegative
    lymphaticmetastasis ( P <0.05 ) .Moreover , thesamplesatⅢ +Ⅳ stageshowedsignificantly
    higherexpressionsofVEGFandHIF-1αthanthatatⅠ+Ⅱ stage ( P <0.05 ) .Conclusion The
    expressionsofVEGF , HIF-1αandCOX-2 werehigherin NSCLC , indicatingthatthethree
    antigensplayedimportantrolesinNSCLCdevelopment.
    Clinical study on the reasons for retreating postoperative spinal tuberculosis#br#
    YAO Li-ming1, ZHAO Qian2, JIA Chen-guang1*,LIU Feng-sheng1, LI Zhuo1, DONG Zhao-liang1
    2016, 37(3):  260-263.  doi:10.3969/j.issn.10073205.2016.03.004
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    [
    Abstract ] Objective To summarizethe reasonsforretreating postoperative spinal
    tuberculosis ( TB ) .Methods Twenty-threepatientswithpostoperativerecurrenceofspinalTB
    (
    observationgroup ) and186patientswithnorecurrenceofspinalTB (
    controlgroup ) were
    studiedretrospectively.Theclinicaldatabetweentwogroupswerecomparedandthecorrelation
    factorsofretreatingspinalTBwerestatisticallyanalyzed.Results Theincidenceofnutrition ,
    degreeofdebridement , graftbonefusion , postoperationalchemotherapyintheobservationgroup
    werelowerthanthoseinthecontrolgroup.Theincidenceofdrugresistance , TBatotherparts ,
    adverseconditionsintheobservationgroupweresignificantlyhigherthanthoseinthecontrol
    group ( P <0.01 ) .Multiplelogisticregressionanalysisrevealedthatpreoperativeserumalbumen
    level ( <35g / L ), nothavingstandardizedpostoperationalchemotherapy , nothavingcompletely
    debridement , withoutfusion , drugresistance , TBatotherpartsandadverseconditionswerethe
    riskfactorsofretreatingpostoperativespinalTB.Conclusion Theearlyandpositivetherapies
    (
    nutritionalsupportandanemiacorrection ), strengtheningtheanti-TBtreatmentatotherparts ,
    complete debridement for lesions , graft bone fusion , standardized anti-TB treatment ,
    strengtheningthepostoperativemanagement ( psoaspuncturewithdrewedeffusionandinjected
    drug , controlofbloodglucose
    ) shouldbehelpfultoreduceretreatingrateofpostoperativespinal
    tuberculosis.
    Theinfluenceofpulsedelectromagneticfieldsonrepairofulnarnerve#br# inpostoperationofmoderatetoseverecubitaltunnelsyndrom
    TIAN Pei-ren1，2，DAI Fu-qiang1，2，XUE Jin-wei2*
    2016, 37(3):  264-267.  doi:10.3969/j.issn.10073205.2016.03.005
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    [ Abstract ] Objective Toexploretheeffectofpulsedelectromagneticfieldsonrepairofulnar
    nerveinpostoperationofmoderatetoseverecubitaltunnelsyndrom ( CUTS ) .Methods Atotal
    of107patients with moderatetoseverecubitaltunnelsyndrom wererandomlydividedinto
    minimally pulsed electromagnetic fields treatment group and control group. Then ,
    electromyography , ulnarnervediameterandcurativeeffectindexweredetectedinpatientsoftwo
    groupsbeforesurgeryandat2 , 4and12weeksaftersurgery.Thestatisticalanalysiswasmade.
    Results Onehundredandsevenpatientswith moderatetosevereCUTSaftersurgery were
    followed-upfor12 weeks.Nerveconductionvelocityintwogroups wasgraduallyelevated.
    Latencyintwogroupswasgraduallydeclined.Amplitudeintwogroupswasgraduallyelevated.
    Thefluctuationrangeintreatmentgroup wasfasterthanthatinthecontrolgroup.The
    differencesbetweengroups , point-in-timeandinterceptwerestatisticallysignificant ( P <0.05 ) .
    Theulnarnervelongdiameterofthelesionsbetweentwogroupshadnosignificantdifference.
    Thedifferencesoftheulnarnervediameterbetweengroups , point-in-timeandinterceptwerenot
    statisticallysignificant ( P >0.05 ) .Theclinicalefficacyofpulsedelectromagneticfieldstreatment
    group was better than thatin the control group ( P < 0.05 ) .Conclusion The pulsed
    electromagneticfieldscaneffectivelypromotetherepairofulnarnerveandthemusclegroupin
    handinpostoperationofmoderatetoseverecubitaltunnelsyndrome.Thistechniquecanreduce
    complicationsandimproveclinicalefficacy.
    TracingeffectofPKH26-labeledhumanumbilicalcordmesenchymal stemcellsinendometriumofrats
    HAN Hua1， ZHAO Hong-wei2， KANG Jin-sheng3， GAO Hai-xiang4
    2016, 37(3):  268-271.  doi:10.3969/j.issn.10073205.2016.03.006
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    [
    Abstract ] Objective Bylabelingthehumanumbilicalcordmesenchymalstemcells ( UC-
    MSCs ) withPKH26 , themigrationofcellinendometriumofratswereobserved.Labelingand
    tracingeffectsofPKH26wereevaluated.Methods IsolatedhumanUC-MSCswerelabeledwith
    PKH26.Thelabeledcellsweretransplantedtoratsthroughcaudalvein.Thenthedistributionof
    cellsinratendometrium wasobservedunderfluorescencemicroscope.Results Therewereno
    detectabledifferencesincellmorphologyandgrowthafterPKH26labeling.Andthecellswere
    mainlylabeledwithredfluorescenceunderinvertedfluorescence microscopeand were mainly
    distributedin epithelialcells and stromalcells , in endometrial glandular epithelium and
    mesenchyme.Conclusion Labelingthehuman UC-MSCswithPKH26isasafeandeffective
    method , whichcanbeusedinthestudyoftransplantationofstemcells.
    Clinical study of effects of compound glycyrrhizin on postoperative adjuvant chemotherapy in ovarian cancer patients
    HUANG Ping1， GUO Liang1， PEI Xin-wei1， YAO Hai-rong1， CHENG Jian-xin2*
    2016, 37(3):  272-274,284.  doi:10.3969/j.issn.10073205.2016.03.007
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    [
    Abstract ] Objective Toinvestigatetheeffectsofglycyrrhizicacidinreducingtheside
    effects , improving clinical symptoms and protecting liver function in the patients with
    gynecologicmalignantovariancancerafterchemotherapy.Methods A totalof60casesof
    patientswithovariancancerwererandomlydividedinto2groups : theobservationgroup ( n =30 )
    andthecontrolgroup ( n =30 ) .Thepaclitaxel ( Toxal160-90 mg
    / m
    2 ,
    Day1 , 8 , 15 ) and
    carboplatin ( Carbo [ AUC=5 ], Day1 ) wereusedintwogroups.Thechangesofclinicalindexes
    intwogroupswerestudiedafter4cyclesofchemotherapy.Thetreatmentgroupwasgivenwith
    compoundglycyrrhizininjection ( 60 mLin10% glucosesolution250 mLintravenousinfusion ,
    onetimeperdayfor10consecutivedays ), thentakingcompoundglycyrrhizintablet ( 150mgone
    time , 3timesoneday ) .Thechangesofleukocytes , clinicalsymptoms , liverfunction , CD3
    + ,
    CD4
    + ,
    CD8
    + ,
    CD4
    + /
    CD8
    +
    andprognosisinpatientsbeforeandafter4cyclesofchemotherapy
    weremonitored.Results There weresignificantdifferencesintheincidenceofleukopenia ,
    improvingtheabnormalliverfunction , theclinicalsymptomsandchangesofCD3
    + , CD4 + ,
    CD8
    + ,
    CD4
    + /
    CD8
    +
    betweentwogroups ( P <0.05 ) .Conclusion Compoundglycyrrhizincan
    improvethesidereaction , liverfunction , clinicalsymptomsandimmunosuppressioninovarian
    cancerpatientsduringchemotherapy.However , ithasnoeffectontumorprognosis.
    Observation on ultrastructure of lensinduced myopia in guinea pig#br#
    ZHANG Xin1， WANG Chao-ying2
    2016, 37(3):  275-279.  doi:10.3969/j.issn.10073205.2016.03.008
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    [ Abstract ] Objective Tostudytheultrastructurechangesofmyopiainducedbylens-induced
    methodandtoexploreitspathologicalmechanism.Methods Thirty-nineguineapigswithtwo
    weeksoldwererandomlydividedintothreegroups : groupA , groupBandgroupC , withthirteen
    ratsineachgroup.Aeyewasrandomlyselectedtowear-10.00DSconcavelens , whichwas
    removedafterculturingfor10days , 30daysand50daysingroupA , groupBandgroupC ,
    respectively.Two-week-oldguineapigswerelens-inducedmonocularly.Atday10 , day30andday
    50 , thelenswereremovedrespectively.After measurementofreferactionandaxiallength ,
    eyeballswereremovedandtheeyeswereobservedbylightandelectronmicroscope.Results
    Aftertheexperiment , theexprerimentaleyesandthecontroleyeswereinduced myopia , the
    growthoftheeyeaxis , thedifferencewasstatisticallysignificant ( P <0.05 ) .Andwiththetime
    prolonged , themoreseveretheeyes , themoreserioustheaxiallength (
    P <0.05 ) .Therefractive
    degreeoftheexperimentaleyewaslowerthanthatofthecontrol , andtheaxiallengthwaslonger
    thanthatofthecontroleye ( P <0.05 ) .Thereweresignificantdifferencesamongthe3groupsin
    thescleralthickness ( P <0.05 ) .ThethicknessofgroupCwasthinnerthanthatofgroupA ( P <
    0.05 ) .Comparedwiththecontroleyes , thescleralthicknessingroupCwasthinnerthanthatin
    controlgroup ( P <0.05 ) .Thecollagenfibersinthegroup A wereslightlydisordered.The arrangementofcollagenfibersingroupBwereslightlydisordered , thicknesswasuneven , and
    gapsbecamelarger.IngroupC , thedisorderofcollagenfibersanduneventhicknessofthe
    collagenfiberswereincreased , andthestructureofcollagenfiberswasnotclear.IngroupC , the
    outernuclearlayeroftheretinabecamesmallerandround , andarrangementwasdisordered.
    Conclusion Theguineapigcanbeusedasaneffectiveandeconomicalmanmmalmodelin
    experimental myopia study.The degenerations were occurredin sclera and retinain the
    experimentalmodels.
    The experimental study of effects of Bushen Huoxue Guchi formula on BMP2 and  TRPV5/TRPV6 channels expressions in MC3T3E1 cells
    GAO Yi1, MU Chun-hui2, DIAO Zhi-hong3*, LI Min1, WANG Shuang-shuang1, YANG Lin1
    2016, 37(3):  280-284.  doi:10.3969/j.issn.10073205.2016.03.009
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    [Abstract ] Objective ToobservetheeffectofBushen HuoxueGuchiFormulaonbone
    morphogeneticprotein2 ( BMP2 ) andTRPV5 / TRPV6channelexpressions , andtoexplorethe
    mechanism ofBushen Huoxue GuchiFormulainboneformation.Methods Herb-contained
    serum waspreparedfromratsbygavagewithBushen HuoxueGuchiFormulabyusingbody
    surfaceareaconvertedequivalentdose.Blank-containedserumascontrolgroupwaspreparedby
    gavagewithnormalsaline.TheBMP2andTRPV5 / TRPV6mRNAexpressionsweredetectedin
    MC3T3-E1cellsby RT-PCRafterthecellsweretreated with10% herb-containedserum or
    blank-containedserumfor24h , 48hand72h , respectively.Results BMP2 , TRPV6mRNA
    weresignificantlyincreasedin herb-containedserum treatmentgroupcompared with blank-containedserum treatmentgroup ( P <0.05 ) .Withtheprolongation oftime , the mRNA
    expressionof BMP2 and TRPV6 had a graguallyincreased tendency , butthere was no
    statisticallysignificantdifference ( P >0.05 ) .TRPV5 mRNA wasnotdetectedeitherinherb-
    containedserumtreatmentgrouporblank-containedserumtreatmentgroup.Conclusion Bushen
    HuoxueGuchiFormulamayupregulateBMP2expressionthroughTRPV6channelstoinduce
    differentiationofosteoblast.
    EffectofAlphamusiconanxiety , depressionandcognitiondysfunction#br# forpatientswithcognitiveimpairmentfollowingstroke
    MA Jiang1， HUANG-Jie1， HAN Zhen-ping1*， JIA Zishan2， ZHANG Lingling1， LIANG Na1
    2016, 37(3):  285-288.  doi:10.3969/j.issn.10073205.2016.03.010
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    [
    Abstract ] Objective Toexploretheeffectsofalphamusiconanxietyanddepression , as
    wellascognitiondysfunctionforpatientswithcognitivedeficitfollowingstroke.Methods Sixty
    patientsfollowingstrokewithcognitiveimpairmentwererandomlydividedintotwogroupsby
    admissionorder : experimentalgroup ( 30cases ) andcontrolgroup ( 30cases ) .Allpatientsreceived
    routinerehabilitationtherapyandnootropicdrugs.Patientsinexperimentalgroupreceivedalpha
    musicforfourweeks.Singleblindassessmentwasdoneusingthe HAMA14 , HAMD24and
    MoCAbeforetreatmentand4 weeksaftertreatment.Results Beforetheintervention , the
    anxietyanddepressionintwogroupswasnotsignificantlydifferent.However , theanxietyand
    depressioninpatientsafterinterventionwerelowerthanthosebeforetheinterventionorthosein groupswerenotsignificantlydifferent.Inobservationgroup , aftertheintervention , theMoCA
    totalscore , memory , attentionabilitywerehigherthanthosebeforetheintervention , orthosein
    controlgroup ( P <0.05or<0.01 ) .Conclusion Alphamusiccanimproveanxiety
    , depression , as
    wellascognition , especiallythememoryandattentionabilityinpatientswithcognitivedeficit
    followingstroke.
    controlgroup.Beforetheintervention , MoCAtotalscore , memory , attentionabilityintwo
    Inhibitioneffectsof2-methoxyestradiolonmelanomaxenografttumorinvivo
    HU Cai-xia1, JIANG Shao-qian1, ZHANG Guo-qiang1, FENG Jia2, TIAN Fei3, GAO Shun-qiang1*
    2016, 37(3):  289-292.  doi:10.3969/j.issn.10073205.2016.03.011
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    [
    Abstract ] Objective Tostudytheinhibitioneffectsof2-methoxyestradiol ( 2-ME ) on
    malignantmelanoma ( MM ) cellsinBALB / c micexenografttumor.Methods Theinhibition
    effects of 2-ME on MM cells were measured by 3- ( 4 , 5-Dimethylthiazol-2-yl ) -2 , 5-
    diphenyltetrazoliumbromide ( MTT ) .BuildingBALB / cmicexenografttumormodel , BALB / c
    miceweredividedintothreegroupswithsixmiceineachgroup.Treatmentgroupandnegative
    controlgroupweretreatedwith2-ME ( 50mg /
    kg
    ) andequallyvolumenormalsalinerespectively
    everytwodays , fora14-daysdurationandcalculatedtheaveragevolumeeverythreedays. PositivedruggroupweretreatedwithCTX ( 20 mg /
    kg
    ) .Afterexperimentended , thetumor
    weightwerecalculated.TheexpressionlevelsofC-mycandGadd45bintumorweredetectedby
    immunohistochemical.Morphologicalchangesoftumortissue , lung , liverandspleentissues
    wereobservedbyHEstaining.Results 2-MEhadobviousinhibitoryeffectonmelanomacells
    andtheinhibitoryeffectinadose-timedependentmanner.ComparedwiththeNaclgroup , the
    volumeandweightoftumortissuesinCTXgroupand2-MEgroupwerereducedremarkably
    (
    P <0. 01 ), buttherewasnostatisticalsignifieancebetweentheCTXgroupandthe2-MEgroup
    (
    P >0.05 ) .Comparingtocontrolgroup , theexpressionlevelsofC-mycweredecreased , while
    Gadd45bwereincreased.Noobviousmorphologicalchangeswerefoundinliverandspleentissues
    intreatmentgroupandincontrolgroup , buttherewerelungmetastasisin3of6mice.Conclusion
    2-MEhadinhibitoryeffectonBALB / cmicexenografttumor , andthe mechanism maybe
    relatedtomodulatinggadd45bandC-mycgenesexpression.
    The influence of the pain management on the postherpetic neuralgia patients
    DU Yu-min1, REN Yu-e1*, CONG Hai-jing1, JING Feng-min2, Xi Lu-lu1
    2016, 37(3):  293-295,300.  doi:10.3969/j.issn.10073205.2016.03.0012
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    [
    Abstract ] Objective Toinvestigatetheeffectofpain managementpathforpostherpetic
    neuralgia.Methods Nintypatientswithpostherpeticneuralgiaweredividedintotwogroups
    randomly : thecontrolgroupandtheexperimentalgroup with45casesineachgroup.The
    patientsinthecontrolgroup weretreatedwithconventionaltreatmentandnursingcare , the
    patientsintheexperimentalgroupweretreatedwiththepainmanagementpath , basedonthe
    conventionaltreatmentandnursingcare.Thedegreeofpainscore , theinterferencedegreeof
    sleep , adverseeffectsofthetwogroupsandtheoverallsatisfactionofpatientswerecomparedat
    day5andday10aftertreatment.Results Therewasnostatisticallydifferentbetweenthetwo
    groupsonthedegreeofpainandtheinterferencedegreeofpatients'sleepbeforetreatment.At
    day5andday10aftertreatment , thedifferenceofthetwogroupsonthedegreeofpain , the
    interferencedegreeonsleep , theoccurrenceofconstipationandtheoverallsatisfactionofpatients
    werestatisticallysignificant ( P <0.05 ) .Conclusion Theapplicationofthepainmanagementfor
    thepatientswithpostherpeticneuralgia , canrelievethepain , improvesleepqualityandthe
    overallsatisfaction , theoccurrenceofadverseeffectsalsocanbereduced.
    Expression of miR16 in plasma and synovial fluid of patients with rheumatoid arthritis
    FENG Zhong-jun1, FENG Yan-rui1, WEN Hai-feng1, SUN Yin2
    2016, 37(3):  296-300.  doi:10.3969/j.issn.10073205.2016.03.013
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    [
    Abstract ] Objective ToobservetheexpressionlevelsofmiR-16inplasmaandsynovial
    fluidfrompatientswithrheumatoidarthritis ( RA ), andtoexploreitsvalueinearlydifferented
    diagnosisandin predictionofdiseaseseverityof RA.Methods Plasma , peripheralblood
    mononuclearcell ( PBMCs ) andsynovialfluidweretakenfrompatientswithRAandosteoarthritis
    ( OA ) .The expression level of miR-16 was detected by stem-loop real-time fluorescent
    quantitativepolymerasechainreaction ( stem-loopFQ-PCR ) withSYBRGreenⅠdye , andusing
    U6snRNAasendogenouscontrol.Results Bothoftheamplicationcurvesandthedissociation
    curvesofmiR-16andU6snRNA wererunningwell.Noprimerdimmerswereformed , andthe
    productsofPCRwerespecific.TheexpressionlevelofmiR-16inplasmawashigherthanthosein
    synovialfluidandPBMCs , respectively ( P <0.01 ) .TheexpressionlevelofmiR-16inPBMCs
    washigherforRApatientsthanforhealthycontrols ( P <0.01 ), thelevelinsynovialfluidwas
    higherforRApatientsthanforOApatients ( P <0.01 ) .TheexpressionlevelofmiR-16inplasma wasinverselycorrelated withtheDisease ActivityScore ( DAS28 )( P <0.05 ), butwasnot
    correlatedwitherythrocytesedimentationrate ( ESR ) andC-reactiveprotein ( CRP ) .themiR-16
    levelinPBMCswaspositivelycorrelatedwithDAS28 , ESRandCRP ( P <0.05 ) .ThemiR-16
    levelinsynovialfluidhadnocorrelationswithDAS28 , ESRandCRP ( P >0.05 ) .Conclusion
    Stem-loopFQ-PCR with SYBR Green Ⅰdye wasasensitiveandspecific methodforthe
    quantitativedetectionofmiRNAs.Thereweredistinctprofilesinplasmaandsynovialfluid , and
    theexpressionlevelofmiR-16insynovialfluidcanbeamarkerfordifferentialdiagnosisofRA
    andOA.Theexpressionlevelofplasma miR-16canserveasaneffectivenovelindicatoron
    assessmentoftheclinicaldiseaseactivity.
    Effects of dexmedetomidine on lung NF-κB expression and the plasma TNF-α as well as ICAM-1 levels in patients undergoing one lung ventilation
    GUO Qiong-mei1, ZHOU Chang-hao1*, ZHANG Jian-bo2, CHEN Huan1
    2016, 37(3):  301-305.  doi:10.3969/j.issn.10073205.2016.03.014
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    [
    Abstract ] Objective Toobservetheeffectsofdexmedetomidineonlungnuclearfactor-κB
    ( NF-κB ) expressionandtheplasmatumornecrosisfactor-α ( TNF-α ) andintercellularadhesion
    molecule-1 ( ICAM-1 ) levelsinpatientsundergoingonelungventilation ( OLV ) andtoinvestigate
    themechanism oflungprotection.Methods Fortylungcancerpatientsscheduledforradical
    operationwererandomlydividedinto2groups ( n =20ineachgroup ) byusingarandomnumber
    table : controlgroupanddexmedetomidinegroup.Indexmedetomidinegroup , dexmedetomidine1
    μ g
    / kgwasinfusedintraveineusly (
    iv ) after10minanaesthesiainduction , andthenwasinfusedat
    arateof0. 5 μ g ·
    kg
    -1 · h -1 until30minaheadoftheendofoperation.
    Incontrolgroup , theequal volumeofnormalsalinewasinfused.Arterialbloodsampleswerecollectedimmediatelybefore
    OLV ( T 1 ), 60minafterOLV ( T 2 ), 30minafterbilateralpulmonaryinflating ( T 3 ), 2hafter
    operation ( T 4 ) and24hafteroperation ( T5 ) toobtainarterialbloodgasanalysisdata , oxygen
    index ( OI ), respiratoryindex ( RI ), PaO 2 / PAO 2 andtodeterminelevelsofplasmaTNF-αand
    ICAM-1.Lungtissueswereobtainedmorethan5cmawayfromtumoratthetimeoflobectomy
    oflungsinordertodetermineNF-κBexpression.Results ThevaluesofOIandPaO 2 / PAO 2 were
    decreasedatT 2 ~T 4 thanthatofatT 1 intwogroups.ThevaluesofOIandPaO 2 / PAO 2 were
    increasedindexmedetomidinegroupcomparedtothatincontrolgroupatT 2 ~T 4 ( P <0.05 ) .The
    valuesofRIwereincreasedatT 2 ~T 4 thanthatofatT 1 intwogroups , andweredecreasedin
    dexmedetomidinegroupcomparedtothatincontrolgroupatT 2 ~T 4 ( P <0.05 ) .PlasmaTNF-α
    and ICAM-1 levels showed ascending and descending trends , and were decreased in
    dexmedetomidinegroupcomparedtothatincontrolgroupatT 2 ~T 4 ( P <0.05 ) .Theexpression
    ofNF-κBwasincreasedindexmedetomidinegroupcomparedtothatincontrolgroup ( P <0.05 ) .
    Conclusion Dexmedetomidinewith1 μ g / kgloadingdoseand0.5 μ g ·
    kg
    -1 · h -1 continuing
    infusiondose during operation caninhibitactivation of NF-κB and reduce OLV induced
    inflammatoryresponseaswellasimproveoxygenationfunctionandshowitslungprotection
    roles.
    PreparationandapplicationofRanitidinehydrochloride#br# electrodebasedonfilmsofmolecularlyimprintedpolymers
    SHEN Zhi-guo1,2, HUANG Bi-xia1, CHEN Peng-yu1, XIU Rong1*
    2016, 37(3):  306-308,312.  doi:10.3969/j.issn.10073205.2016.03.015
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    [ Abstract ] Objective To establish a new method for determination of ranitidine
    hydrochloridedrugcontent.Methods Byusingranitidinehydrochlorideastemplate , andusingo-
    pheny-lenediamineaswellasmethacrylicacidasfunctionalmonomer , wepreparedthemolecular
    imprintedpolymermembranesensorofranitidinehydrochlorideonthesurfaceofaglassycarbon
    electrodeby using cyclic voltammetry ( CV ) electropolymerization method.Electrochemical
    behaviorofthesensorwascharacterizedbyCV anddifferentialpulsevoltammetries ( DPV ) .
    Results ThepeakcurrentsofDPVandconcentrationhaveagoodlinearrelationshipintherange
    of3. 010×10
    -7 -6.
    172×10
    -6
    mol
    / L , andthelinearequationwasIp (
    μ A ) =-2.
    423c+0. 00002
    (
    r =0.9951 ) .Conclusion Thesensordisplayedgoodselectivity , excellentreproducibility ,
    stability , accuracy , anditcouldbeusedtomeasureranitidinehydrochloridedrugactualcontent.
    Effectsofcontinuouslytakingmetoprololtartrateonthepharmacokineticsinrats
    ZHANGYa-kun,WANG Wei-mei,GONGYing,ZHANG Huan,ZHANGZhi-qing*
    2016, 37(3):  309-312.  doi:10.3969/j.issn.10073205.2016.03.016
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    ( DepartmentofMedicine , theSecondHospitalofHebeiMedicalUniversity , Shijiazhuang 050000 , China )
    [
    Abstract ] Objective Toinvestigatethechangesofcontinuousadministrationofmetoprolol
    tartratetabletsinsixdaysonthepharmacokineticofinrats.Methods Fortyratswererandomly
    dividedintotwogroups with20ratsineachgroup.Eachratinthetestgroup wasgiven
    metoprololtartrate ( 27mg /
    kg
    ), andeachratinthecontrolgroupwasgivenequalvolumeof
    distilledwaterorally , twicedailyfor6days.Onday7 , eachratintwogroupsrecievedasingle
    doseof metoprololtartrate ( 27 mg /
    kg
    ) .Bloodsamples weretakenatdifferenttimesafter
    administrationfromtheinnercanthus.Theconcentrationsofmetoprololtartrateweredetermined
    byultraperformanceliquidchromatography ( UPLC ) .Thepharmacokineticparametersofthetwo
    groupswerecalculatedwithDAS2. 1. 1pharmacokineticsoftwareandwerecomparedwithSPSS
    13. 1software.Results The mainpharmacokineticparametersinthetestgroupandcontrol
    group : areaundercurve ( AUC ) 0-5h were ( 0. 84±0. 40 )
    mg
    · h
    -1 · L -1 and (
    0. 96±0.46 )
    mg
    · h
    -1 ·
    L
    -1 ,
    respectively ; AUC 0-∞ were (
    0. 91±0.41 )
    mg
    · h
    -1 · L -1 and (
    1. 05±0. 50 )
    mg
    · h
    -1 · L -1 ,
    respectively ; halflife ( t 1 / 2 ) were (
    1. 23±0.36 ) hand ( 1. 42±0.41 ) h , respectively ; T max were
    (
    0. 51±0.10 ) hand ( 0. 85±0.28 ) h , respectively ; V were ( 58.19±22.61 ) L / kgand ( 61. 54±