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Table of Content

    25 June 2014, Volume 35 Issue 6
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    论文
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    2014, 35(6):  621-621. 
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    Objective To explore the relationship of telomerase activity of human luteined granulosa cells( GCs)with ovarian response and embryo quality. Methods Fity-six cases receiving IVF-ET were used to collect the data of GCs. The experiment was carried out through the following procedures:luteined GCs were isolated from follicle fluid and were cultured 24 hours soon;tests of the telomerase activity of GCs with TRAP-ELISA. Comparisons of age,number of ampules of gonadotropin ( Gn ) administered,number of retrieved oocytes,rate of high quality embryo with telomerase activity were made in different ovarian response and embryo quality groups. Results Isolated and purified GCs were cultured and adherenced well in 24 hours. There were negative correlation between telomerase activity of GCs with age,number of ampules of Gn administered and positive correlation with number of retrieved oocytes( P<0 . 05 ). Low response group had lower telomerase activity in GCs compared to medium and high response groups( P<0 . 01 ). There were positive correlation between telomerase activity of GCs with ratio of high embryo quality( P<0 . 01 ). There was significant difference of telomerase activity of GCs in different embryo quality groups( P<0 . 01 ). Conclusion Telomerase activity could be tested in human luteinized GCs after in vitro culture. It may suggest that telomerase activity decreased with the decline in ovarian function and the apoptosis of GCs may directly affect oocyte developmental potential.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    ZHU Jianzhong;LIU Ji;WANG Guoming;WAGN Juan;SUI Yuelin;SHI Geming
    2014, 35(6):  625-625. 
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    Objective To study the expression of 5-hydroxytryptamine( 5-HT ) and double-labeled neurons in dorsal raphe of cirrhosis rats. Methods The healthy male Wistar rats were divided into model and control groups(n=10). Within 14 weeks,model group rats were subcutaneously injected by CCl4 plus drinking ethonal. Control group rats received subcutaneously injection of sodium chloride. 5-HT immunohistochemistry and 5-HT immunohistochemistry combined with NADPH-d staining were used to detect the expression of 5-HT and double-labeled neurons in dorsal raphe of rats. Results The average diameter 5-HT neurons in dorsal raphe decreased significantly in the model group compared with that of control group. The optical density value of 5-HT neurons in dorsal raphed increased sinificantly in the mode of group(0. 54 ± 0. 04)compared with that of control group(0. 48 ± 0. 04)(P <0. 01). The quantity of 5-HT neurons and the double-labeled neurons in dorsal raphe hasd no significant difference between two groups. Conclusion Cirrhosis influenced 5-HT neurons in dorsal raphe of rats.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    CHEN Bo
    2014, 35(6):  628-628. 
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    Objective To explore the clinical features and treatment methods of isolated coronary artery ectasia ( CAE ) at high altitude by coronary arteriography. Methods Coronary arteriographic images and clinical data of 912 cases from high altitude( 2 260 - 4 500 m ) were retrospectively analyzed. Results Thirty-two isolated CAE cases were found in 912 coronary arteriographic populations with the incidence of 3. 5%. Right coronary artery(26 cases,41. 3%),left main coronary artery(17 cases,27%),anterior descending branch(13 cases,20. 6%)and circumflex(7 cases,11. 1% ) were the predilection sites in turn. Hyperlipidemia,drinking,hypertension, hyperglycemia,altitude and hemoglobinre were the independent risk factors of isolated CAE. Eight cases had still had angina in the half year of follow-up. Conclusion Except the risk factors with coronary heart disease,altitude hypoxia is another factor of isolated CAE. Antiplatelet drugs,calcium antagonists,statins and trimetazidine need long time use for the patients. CAE drug or intervention in high-risk patients who can not control the need for surgical ligation or resection of hemangioma,at the same time the need for bypass operation of coronary artery.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    LIU Yongliang;WANG Lijuan;WANG Lina;LI Jianmin;ZHAO Yaning;WANG Peng
    2014, 35(6):  631-631. 
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    Objective To explore the effect of butylphthalide on the brain edema of rats following traumatic brain injury and the expression of p38MAPK around the injury area. Methods 144 Sprague-Dawley male rats were divided into sham group,control group and butylphthalide group(40mg/kg,once a day). The model was made by Marmarou method. After injury 1,2,7,14d,behavior scores were done to observe the changes of neuromotor function,brain water content was measured to observe the changes of the brain edema,the immunohistochemistry and western blot were done to look the expression of p38MAPK around the injury area. Results Compared with the sham group,the behavior scores of control group were decreased,the brain water content of the control group was more,the content of p38MAPK around the injury area was increased( P <0. 05 ). Compared with the control group,the behavior scores of butylphthalide group were increased,the brain water content of the butylphthalide group was less,the content of p38MAPK around the injury area of butylphthalide group was decreased( P <0. 05 ). Conclusion Butylphthalide could reduce the expression of p38 MAPK following traumatic brain injury and reduce the brain edema.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    DONG Haipeng;GAO Shijun;DOU Yan;ZHENG Xiaozuo;ZUO Baijun
    2014, 35(6):  635-635. 
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    Objective To study the biomechanic characteristics of knee joint after cartilage repairing with platelet-rich plasma( PRP). Methods The models of cartilage injury on weight-bearing area in the medial femoral were constructed,and were respectively repaired by PRP and normal saline ( NS). The uninjured cartilages were regarded as control group. The repair of injured cartilage were examined,and the medial and lateral contact area and pressure of the knee joint were recorded 1,3,5 weeks postoperatively. Results The newly repaired cartilage was observed in PRP group,and the reparative effect was better than the NS group 1,3,5 weeks postoperatively. There were no significant difference in the fields of the contact area and pressure of the medial and lateral the tibiofemoral joint five weeks after operation between the PRP group and the control group( P>0 . 05 ). Conclusion The PRP was a potential factor for repairing injured cartilage. The contact area and pressure of the knee joint after repair by PRP were similar to the normal cartilage.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    LIU Jinfeng;ZHOU Xiaohui;LIU Qiang;LI Hongfang;WANG Yuncang;TIAN Qing
    2014, 35(6):  639-639. 
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    Objective To study the effect of ulinastain on early postoperative lung function of esophageal cancer patients. Methods The study selected 50 esophageal carcinoma in-patients who were randomly divided into two groups. Ulinastain group(25 cases):normal saline 250mL +ulinastatin one million unit by intravenous injection,twice a day from the operation day to five days after the operation. The control group(25 cases):normal saline 250mL by intravenous injection,twice a day,from the operation day to five days after the operation. Comparison was made in postoperative lung function index forced expiratory volume in the first second( FEV1 ),diffusion capacity for carbonmonoxide( DLCO)and partial pressure of oxygen( PO2 )of the two groups. Results Postoperative lung function indexes of two groups declined obviously compared with preoperative( P <0. 05 );the two groups in comparisons, postoperative lung functions of the ulinastain group index FEV1 ,DLCO,PO2 of ulinastain group were significantly better than these of control group(P<0. 05);In ulinastain group,there was a case of pain and red at the the location of injection,and a case of dispersed erythra at the trunk after using ulinastatin, the two patients took a favourable turn after stopping injection. Conclusion Ulinastatin can improve early postoperative pulmonary ventilation function,dispersion function,improve oxygenation index,showing positive significance to recovery of postoperative patients′lung function.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    XUE Wenyong;QI Jinchun;YANG Caiyun;ZHAO Xin;GAO Qiang;LI Wei
    2014, 35(6):  642-642. 
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    Objective To investigate the clinical effect of modified Koyanagi technique for the repair of severe hypospadias. Methods We retrospectively analyzed 35 patients with severe hypospadias treated by modified Koyanagi technique. And for comparison,34 cases of severe hypospadias treated with one-stage Duckett+Duplay technique in the same term were collected. Results The operation time of modified Koyanagi technique was much shorter than one-stage Duckett+Duplay technique,the result had statistically significance ( P < 0 . 05 ). The successful rate and complication in two groups had no statistically significance( P>0 . 05 ). The patients with urethral fistula were treated successfully with the repair of urethral fistula 6 months after the first surgery. And the cases of stenosis were cured by urethral dilatation within 1 to 3 months. All patients of the two group were followed up 6 to 24 months( mean 15 months). The correction of chordee and the micturition were very well,and did not need urethral dilation in 6 months at least. Conclusion Modified Koyanagi technique for the repair of severe hypospadias is relatively simple,has similar effect of surgery compared with Duckett+Duplay technique.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    ZHANG Jing;LI Fengwen
    2014, 35(6):  645-645. 
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    Objective This study explores the influence of four ways in hemostasis on the ovarian function in ovarian cystectomy,including ultrasonic scalpel group,monopolar coagulation group, bipolar coagulation group and absorbable suture group. Methods Two hundred women undergoing ovarian cystectomy were divided randomly into four groups,50 cases each group,according to the hemostasis ways. Then,follicle-stimulating hormone( FSH ),luteinizing hormone( LH ),estradiol( E2 ) were examined,also antral follicle count( AFC)was detected with transvaginal color Doppler ultrasound at the second or third day of menstrual cycle before and after the operation 1 month,3 months,and 6 months,separately. Besides,all cases were followe up to observe the changes in the second menstrual crapms and the sixth-month menstrual condition after surgery. Results E2 level of all cases showed the trends of decrease and then increase. The decrease level was more apparent in monopolar coagulation and bipolar coagulation group,and it took a relatively longer time to recover. The decrease was the least and the recovery was the fastest,and the level of E2 was close to the preoperative level six months after surgery in absorbable suture group. FSH and LH were all increased apparently in four groups and then decreased slowly,they were more close to the preoperative level after six months of surgery in absorbable suture group. And it was still higher apparently than the preoperative level in monopolar coagulation and bipolar coagulation group after six months. The level of E2 ,FSH and LH of four groups all showed statistical significance among groups,time and the interaction(P<0. 01). The AFC of all the groups was decreased and then recovered to the preoperative level after six months of surgery. The recovery was more rapid in absorbable suture group. Menstrual changes were mainly demonstrated as extended menstrual cycle,a decrease in menstrual quantity,accompanies with occasional menstrual extension,slight hot flashes sweating,and agitation. However,at six months after surgery,the above mentioned symptoms generally disappeared and menstrual returned to normal. There were no patents suffering from amenorrhoea and severe menopausal transition. Conclusion Compared with the hemostasis of ultrasonic scalpel,monopolar coagulation and bipolar coagulation,absorbable suturation in ovarian cystectomy had the advantages of less injury to the ovarian and faster postoperative recovery.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    CAO Lei;LIU Jicun;LIN Na;LI Xiaona;PENG Zhigang;WU Wenjuan
    2014, 35(6):  650-650. 
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    Objective To compare conventional MRI,short-tau inversion recovery( STIR ), multiple-echo image combination gradient-echo sequence( ME2D)and susceptibility weighted imaging (SWI)in acute cervical spine trauma. Methods Conventional T1WI,T2WI,STIR,ME2D and high-resolution SWI were performed in 30 patients with a history of acute cervical spine injury using a 3 Tesla MRI system( Verio,siemens,Germany ). The detection of vertebral body,intervertebral disc and paravertebral ligament injury by T2 WI and STIR were analyzed. The signal intensity in the hemorrhage and normal spinal cord were measured on the same slice of ME2D and SWI,respectively,and the signal intensity ratio of the hemorrhage to normal spinal cord were calculated. Results Compared with T2 WI, STIR sequence had higher detection rate in showing vertebral bodies injuries,interspinal ligament and nuchal ligament injuries(χ2 =21 . 043 ,1 063 . 499 ,859 . 982 ,P<0 . 01 ),the consistency of result of STIR and T2 WI in detecting injury of interspinal ligament and nuchal ligament was poor( Kappa= 0. 336 and 0 . 329 ). There was no significant difference between STIR and T2 WI in detecting injury of intervertebral disc,anterior longitudinal ligament and posterior longitudinal ligament. Compared with ME2D,SWI sequence could show lower signal intensity ratio of the hemorrhage to normal spinal cord,and there were statistical differences(Z=2. 52,P=0. 014). Conclusion Compared with T2WI,STIR has significant advantage in detecting injury of vertebral body and posterior ligament complex,so STIR should be included in the routine MRI sequence of acute cervical spine trauma;SWI is more sensitive than T2 WI and ME2 D in showing and confirming hemorrhage in acute cervical cord injury and should serve as important complement sequence for acute spinal cord injury.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    WANG Yan;MA Wenze;LI Yuxia;WANG Guiqi;WEN Yabin;MA Pengyu
    2014, 35(6):  654-654. 
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    Objective To investigate the clinic application of transumbilical single-incision laparoscopic surgery in treatment of upper digestive tract perforation. Methods By reviewing and analyzing the clinic data of transumbilical single-incision laparoscopic( 50 cases ) and traditional laparoscopic( 50 case ),the operation time,pneumoperitoneum time,postoperative intestinal peristalsis recovery time,hospitalization time,intraoperative bleeding volume,postoperative analgesia,wound infection rate,and postoperative follow-up survey of two groups were compared. Results The observation group was better in the aspects of operation time,pneumoperitoneum time,bleeding volume,recovery time of intestinal peristalsis,application of analgesic drugs than the control group(P<0. 01),but the wound infection rate,hospitalization time showed no significant difference between two groups. Conclusion Transumbilical single-incision laparoscopic surgery in the upper digestive tract perforation repair is safe and feasible. It is worthy of clinical promotion and application.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    HAO Juanjuan;ZHANG Zekun;ZHANG Hua;HUO Jiawen
    2014, 35(6):  658-658. 
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    Objective To discuss the pathognomonic performance of appendiceal mucinous cystadenoma detected by multidetector CT( MDCT ). Methods Retrospective analysis was made on MDCT image of eight patients with appendiceal mucinous cystadenoma. Results The appendiceal mucinous cystadenoma in the eight patients was in the right lower quadrant within the caecum,and the near-end of lesion was linked with caecum while the far-end was invisible. Appendiceal lumen was widened in different sizes. Diameter of each lesion in 8 patients was over 2. 5cm. Seven of the lesions showed oval or elongated monolocular change and 1 lesion showed delicate separation. Eight capsule walls were smooth both inside and outside,either thin or thick. Four inner walls had strip-type or eggshell-type calcification. CT value of inner tissues was about 13 to 21Hu,2 discontinuous appendiceal lining were coupled with pseudo-mucotumor in pelvic cavity. No inflammation was detected around 6 lesions,which were not wrapped by omentum and no swelling lymphonodus were detected in mesentery. Cyst walls after contrast enhancement and septums were seen strong enhancement while inner capsule tissues were not seen enhancement. Conclusion Through multiplanar reformation,MDCT could be make a direct and correct diagnosis of appendiceal mucinous cystadenoma.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    TIAN Haiyan;DONG Shumei;DI Xueping;XU Lifeng;LI Yan
    2014, 35(6):  661-661. 
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    Objective To explore the treatment of lung lesions close to the pleural tuberculosis in different time by two-dimensional ultrasound and color Doppler image performance. Methods The data of clinical diagnosis of pleural tuberculosis cases were collected,ultrasound check was performed during anti-tuberculosis treatment,comparison was made in pulmonary tuberculosis treatment of 1 month, 3 months,12 months two-dimensional ultrasound and color Doppler image characteristics. Results In 86 cases close to the pleural tuberculosis ultrasonic signs,different time two-dimensional sonographic findings showed the dynamic change,lung pathological changes of the corresponding color altered from rich to less Doppler blood flow signals. And accompanied pleural effusion in some degree reduced. Conclusion Tuberculosis can be detected before the treatment with a variety of ultrasonographic signs,ultrasonographic signs gradually reduced,disappeared after treatment. Color Doppler ultrasound can real-time observe and measure the scope of pleural tuberculosis echo,blood flow and the dynamic change before and after the treatment,it is helpful to evaluate the effect of tuberculosis treatment,and it can avoid X-ray and CT radiation damage to human body.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    LI Yan;YANG Tao;TIAN Haiyan
    2014, 35(6):  664-664. 
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    Objective The study is to explore the application of transient elastography ( FibroScan)and biological indexes on nonalcoholic fatty liver disease( NAFL)patients. Methods 313 NAFL patients and 300 normal healthy people underwent abdominal ultrasound examination,biochemical examination and FibroScan. Results The body mass index( BMI ),alanine aminotransferase( ALT ), aspartate aminotransferase( AST),fasting blood glucose( FBG),total cholesterol( TC),triglyceride( TG), low density lipoprotein cholesterol( LDL-C ),glycosylated hemoglobin( HbA1 c ),liver stiffiness( E )and controlled attenuation parameter( CAPTM )in NAFL group were higher than those of the normal group (P<0. 01),but high density lipoprotein cholesterol(HDL-C)was lower than that of normal group(P<0 . 01 ). FibroScan examination showed higher diagnostic accuracy for NAFL than ultrasonography( P <0. 01),CAPTM was positively correlated with TG,BMI,FBG,HDL-C,E( P <0. 05 or P <0. 01 ). Conclusion CAPTM in FibroScan is a non-invasive,painless quantitative tool in assessment and monitor of fatty liver.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    ZHU Qian;ZHANG Yujie;TIAN Ming;CUI Lingli
    2014, 35(6):  668-668. 
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    Objective To observe the hemodynamic changes of anesthesia inducement of etomidate combined with propofol in elderly patients during general anesthesia. Methods Total 100 elderly patients with general anesthesia were randomly divided into five groups of propofol( group P ), etomidate( group E ),propofol combined with etomidate 1( group PE1 ),2( group PE2 )and 3( group PE3 )according inducement agents. The systolic blood pressure( SBP),diastolic blood pressure( DBP), mean arterial pressure( MAP),heart rate( HR)and bispectral index( BIS)value were recorded before inducement of anesthesia( T0 ),before laryngoscope insertion( T1 ),instant( T2 ),1min( T3 ),3min (T4),5min(T5)and 10(T6)min after intubation in each groups. Results Compared with T0,SBP, DBP,MAP and HR at T1 were significantly reduced in five groups(P<0. 05);SBP,DBP and MAP were significantly reduced in group P and PE2(P<0. 05),and DBP,MAP,HR in group PE1 were not different from that of at T2(P>0. 05);SBP,DBP and MAP in group P,E and PE1 were not different from that of at T3(P>0. 05),HR in group E and group PE3 were significantly elevated(P <0. 05). SBP,DBP and MAP at T1 -T3 and HR at T3 in group E were significantly higher than that in group P( P <0. 05 );SBP,DBP and MAP in group PE1 at T1 -T4 were higher than that in group P(P<0. 05),and HR at T3 in group PE1 was lower than that in group E(P<0. 05);At all the time point,SBP,DBP,MAP and HR in group PE2 were not different from those in group P(P>0. 05);SBP,DBP,MAP and HR in group PE3 at T1 -T6 were higher than those in group P(P<0. 05). BIS values in five groups decreased significantly after inducement( P <0. 05 ),and there were no significant changes between groups( P >0. 05 ). Conclusion Administration of propofol 0 . 8 mg/kg combined with etomidate 0 . 15 mg/kg can achieve more stable hemodynamic changes than administration of the two agents individually.
    ZHAO Zhiming;CUI Na;HAO Guimin;XU Suxin
    LIU Xiaolei;ZHAO Jianhong;GAO Zhangquan;QIANG Cuixin;LI Zhirong;YANG Jing
    2014, 35(6):  673-673. 
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    Objective To study and establish the quality evaluation and management system for the clinical laboratories of family planning institutions. Methods Based on the health examination program of pre-pregnancy of the nation,laboratories of the family planning institution were investigated. Relational standards of operation and training system were established. All the test data were analyzed by the external quality assessment system which was developed autonomously. Results Guidance documents were issued,training system was established systematically,EQA system and overlapping evaluation methods related to the clinical laboratories of family planning were developed. Conclusion Together with the laboratories developing,personnel training and composing of guidance documents,quality management system of the family planning laboratories were improved rapidly and completely by the EQA. And the examinations of the health examination program of pre-pregnancy of the nation will be completed better.