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    25 May 2017, Volume 38 Issue 5
    Protective effect and mechanism of probucol on renal ischemiareperfusion injury in mice#br#
    GONG Miao1, ZHANG Lei1, ZHAO Chunfang1, LI Li1, ZHANG Yanan1, REN Guangwei2*
    2017, 37(5):  497-500,505.  doi:10.3969/j.issn.10073205.2017.05.001
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    〗[Abstract] Objective〖HTSS〗〓To investigate the protective effect and mechanism of probucol on renal ischemiareperfusion(I/R) injury in mice.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓10weekold male C57BL/6j mice were randomly divided into four groups(n=10): control group, sham operation group, I/R group, and I/R+probucol(100 mg/kg) group. The renal tissue pathological changes were observed by HE staining and graded. Levels of SCr and BUN in serum were tested by ELISA. The content of MDA and activity of GPXs were detected by using chromometry. The protein expression of GPX4 was measured by western blot.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with control group, pathological changes of renal tissue were aggravated in IR group. SCr and BUN levels as well as MDA content of renal tissue were significantly increased. We also found that GPXs activity and GPX4 protein expression were significantly decreased(P<001). Compared with IR group, histopathological changes of renal tissue were alleviated in IR+probucol group. The levels of Cr and BUN were decreased significantly, with MDA content decreased and GPXs activity or GPX4 protein increased(P<005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Probucol reduces the damage of renal tissue induced by acute ischemia reperfusion injury in mice, which might be involved in activating GPX4.
    Effects of catecholamines on lung injury induced by restraint stress in rats#br#
    LI Li1, ZHANG Xiaojing2, LIU Wei3, SONG Ning4, YU Changli1*
    2017, 37(5):  501-505.  doi:10.3969/j.issn.10073205.2017.05.002
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    Abstract]  Objective〖HTSS〗〓To study the effect of catecholamine(CA) on lung injury in rats with restraint stress.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fifteen adult male SD rats were randomly divided into control group, restraint stress group and restraint+alphamethylptyrosine(AMPT) group, 5 rats in each group. Both plasma norepinephrine(NE), epinephrine(E) and interleukin1 beta(IL1β), interleukin6(IL6) concentration were assayed by enzymelinked immunosorbent assay(ELISA). Hematoxylineosin(HE) staining was used to observe the pathological changes of lung tissue. The protein levels in the bronchoalveolar lavage fluid(BALF) were detected by bicinchoninic acid method.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with the control group, the plasma NE and E levels in the restraint stress group, restraint+AMPT group were significantly higher than those in the control group. Compared with the restraint stress group, the levels of NE and E in plasma of the restraint+AMPT group were significantly lower than those in restraint stress group(P<005). Compared with the control group, the levels of IL1β, IL6 in the lung tissue and BALF protein content of the restraint stress group and restraint+AMPT group were significantly increased. Compared with the restraint stress group, the levels of IL1β, IL6 and BALF in the lung tissue of the restraint+AMPT group were significantly decreased, the difference was statistically significant(P<005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Catecholamine may be involved in the lung injury induced by restraint stress. AMPT can significantly reduce the lung injury induced by restraint stress.
    Observation of curative effect of losartan potassium and hydrochlorothiazide combined with folic acid tablets on Htype hypertension#br#
    AN Lili1, HUANG Wenzheng2, ZHANG Xinmei1, WANG Jihong1
    2017, 37(5):  506-509.  doi:10.3969/j.issn.10073205.2017.05.003
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    [Abstract]  Objective〖HTSS〗〓To observe antihypertensive effect of losartan potassium and hydrochlorothiazide combined with folic acid tablets on Htype hypertension, and to observe the effects on homocysteine(Hcy), folic acid, hyper sensitive Creactive protein(hsCRP), β2microglobulin(β2MG) and cystatin C(CysC). 
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 126 patients with Htype hypertension were enrolled and divided into control group(n=63) and treatment group(n=63). The control group was given losartan potassium and hydrochlorothiazide. The treatment group was given losartan potassium and hydrochlorothiazide combined with folic acid tablets. After eight weeks, we observed antihypertensive effect and changes of serum levels of Hcy, folic acid,hsCRP, β2MG and CysC between both groups.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓There was no significant difference in antihypertensive effect and total effective rate between both groups(P>005). Compared to prior treatment, SBP, DBP, the serum levels of Hcy, hsCRP,β2MG and CysC were obviously decreased, the serum level of folic acid was rised after treatment in both groups. And the changes in the treatment group were better than those in the control group, there was statistically significant(P<005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Losartan potassium and hydrochlorothiazide combined with folic acid tablets obtains an obvious efficacy on Htype hypertension. It can effectively lower blood pressure and improve the serum levels of Hcy, folic acid, hsCRP, β2MG and CysC.
    Clinical effect and possible mechanism of lacol mild hypothermia on acute cerebral infarction
    Song Degang1, Chen Xiaoxue2*, Li Jinglong3, Wang Fang4
    2017, 37(5):  510-513,565.  doi:〓〓10.3969/j.issn.10073205.2017.05.004
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    〗[Abstract] Objective〖HTSS〗〓To examine the effects of  local mild hypothermia therapy on patients with acute cerebral infarction and to further investigate underlying mechanism for the treatment of cerebral infarction.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One hundred cases of cerebral infarction patients were randomly divided into two groups with 50 patients in each group. The control group was given standard medical treatment. The treatment group was given standard medical treatment plus mild hypothermia with a target temperature of 33 ℃ to 35 ℃. Hypothermia was maintained for 24 hours. MRI cerebral infarction volume was observed at the first day and the fifth days after admission. Modified Rankin scale(mRS) scores were measured at 3 months after infarction. The serum levels of superoxide dismutase(SOD) and malondialdehyde(MDA) were assessed before(on admission)and after mild hypothermia therapy(at 72 h after infarction).
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓There was no difference in infarct volume between the two groups(P>005). The concentration of SOD was higher in treatment groups than in control group(P<005) at 72 h after infarction. The concentration of MDA was lower in treatment groups than in control group(P<005) at 72 h after infarction. Patients in the treatment group with good prognosis(mRS<3 scores) were higher than those in the control group, the difference was statistically significant(P<005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Mild hypothermia treatment can improve the prognosis of patients with cerebral infarction, the beneficial effects may be accomplished by reducing oxidative stress reaction.
    The changes of serum hsCRP and LDL Levels in patients with acute cerebral infarction of different TOAST types and its clinical significance#br#
    GU Lili1,2, TIAN Yongwu2, HE Dan1*
    2017, 37(5):  514-519,524.  doi:10.3969/j.issn.10073205.2017.05.005
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    〗[Abstract]  Objective〖HTSS〗〓To investigate the effects of high sensitiveC reactive protein(hsCRP) and low density lipoprotein(LDL) levels in patients with acute cerebral infarction, and to investigate the effects of hsCRP and LDL on the acute cerebral infarction and its clinical significance.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Three hundred and seventyfour cases of cerebral infarction were selected as the case group and 60 healthy volunteers were normal control group. In the case group, 158 patients with largeartery atherosclerosis(LAA), 171 patients with small artery occlusion(SAO) and 45 cases with cardioembolism(CE) were divided into three groups according to TOAST classification. All the subjects were given 3 mL of venous blood in the early morning of the next day, and the levels of serum hsCRP and LDL were measured.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓National Institute of Health stroke scale(NIHSS) score, systolic blood pressure and diastolic blood pressure of LAA, SAO and CE group were significantly higher than those of control group. The NIHSS score of LAA group and CE group was higher than that of SAO group, and the systolic blood pressure of LAA group and SAO group was higher than that of CE group. LAA, SAO group were higher than the control group and CE group. SAO group was higher than the control group, LAA, SAO, CE group of hypertension, hyperlipidemia, heart disease rate was higher than the control group. The incidence of heart disease in CE group was higher than that in LAA group and SAO group. The levels of serum hsCRP in LAA, SAO and CE group were higher than those in LAA group and SAO group, and the level of serum hsCRP in LAA group was higher than that in SAO group. The level of serum LDL in LAA and SAO group was higher than that in control group(P<005). The levels of serum hsCRP in patients with acute cerebral infarction were positively correlated with LDL and NIHSS scores(rs=0.164, 0.183, P<005). Logistic regression analysis showed that systolic blood pressure and hsCRP were risk factors for cardioembolism.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓In the TOAST subtype, serum hsCRP can be used as a risk factor for CE, and it is an indicator of the etiology of early diagnosis of cerebral infarction. LDL is expected to become a specific biochemical index of LAA, which has important clinical value for early prediction of LAA.
    The analysis of correlation between frailty index and postoperative complications of aged patients with nodular goiter #br#
    NIE Yue1,2, LI Shundong2, CHENG Jie2, ZHAN Junkun1, XIANG Jinhua2, LIU Youshuo1*
    2017, 37(5):  520-524.  doi:10.3969/j.issn.10073205.2017.05.006
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    [Abstract]  Objective〖HTSS〗〓To explore the correlation between frailty index and postoperative complications of aged patients with nodular goiter, in order to provide reference evidence for clinical care.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One hundred and twenty cases aged patients with ndular goiter undergoing operative treatment were selected as the research object. All of them were divided into three groups according to the frailty index as follows:nonfrail group(FI<0.08, 35 cases), intermediately frail group(FI≥0.08-0.25, 35 cases) and frail group(FI>0.25, 50 cases). Collect clinical data of patients about general data, BMI, ASA classification, hospital stays and postoperative complications were examined. The correlation between the indexes above and frailty index was analysed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓There was no significant difference between the 3 groups in the ASA classification(P>0.05). Frailty index and the ASA classification has some relevance, but less relevant according to the Spearman correlation analysis( rs=0.265, P=0.007 ). There was significant difference among the 3 groups in the incidence of complications and hospitalization time (P<005). The incidence of complications in the frail group was higher than that in the nonfrail group(P<005). The hospitalization time of intermediately frail group and frail group was longer than that of non weak group. The hospitalization time of the frail group was longer than that of the intermediately frail group(P<005). Spearman correlation analysis showed that FI was positively correlated with perioperative complications(rs=0.519, P=0.023). FI predicts that the ROC curve area of perioperative complications of surgical resection of nodular goiter is 0.812(CI:0.699-0.882, P=0.000).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The level of frailty index is risk factor of postoperative complications of aged  patients with nodular goiter. The preoperative frailty index evaluation can provide reference evidence for the treatment of aged NG patients undergoing surgery with low postoperative complications rate.
    Investigation on the nutritional management of patients with chronic kidney disease
    YANG Yan, DING Faxian, MA Xiao, GUO Junhan, WANG Qinyuan, WANG Jing*
    2017, 37(5):  525-528,534.  doi:10.3969/j.issn.10073205.2017.05.007
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    〗[Abstract] Objective〖HTSS〗〓To investigate the compliance rate of macronutrients of dietary intake, the incidence of protein energy wasting(PEW) and food consumption data in patients with chronic kidney disease(CKD).
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 354 CKD patients hospitalized in Lanzhou University Second Hospital were enrolled in this study. The food consumption data were collected by weighing method combined with 3 consecutive 24hour recalls, and the nutrients intake was calculated by China Food Composition Table. Nutritionrelated indexes were analyzed, which included energy, protein, lipid, carbohydrate, albumin,hemoglobin, body mass index(BMI), incidence of PEW, and consumption data of nine categories food, such as beans, white meat, red meat, eggs, dairy products, staple foods, oils, fruits and vegetables.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓In 354 patients with the stages CKD1-5, the number of patients reached the energy recommendation was 88 and compliance rate was 24.8%. The number of patients reached the protein recommendation was 32 and compliance rate was 9.0%. The number of patients reached the lipid recommendation was 51 and compliance rate was 14.4%. The number of patients reached the carbohydrate recommendation was 88 and compliance rate was 24.8%. The number of patients reached the albumin recommendation was 173 and compliance rate was 48.9%. The number of patients reached the albumin recommendation was 117 and compliance rate was 33.0%. The number of patients reached the BMI recommendation was 165 and compliance rate was 46.6%. In total, these indexes mentioned above were gradually decreased but the incidence of PEW was increased with the progress of CKD. The intake of nine categories food, like beans, white meat, red meat, eggs, dairy products, staple foods, oils, fruits and vegetables was inclined with the progression of the disease. 〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The compliance rate of dietary intake of three macronutrients, such as protein, lipid, carbohydrate, is low and the incidence of PEW is high in patients with CKD. The diets among CKD patients are mainly staple foods and are lack of biological protein, vegetables and fruits in these patients. Consequently,all patients with CKD need to implement target nutrition management by practicing individual dietary guidance and goal nutrition therapy.

    A systematic review of the effect of art therapy on the improvement  of symptoms and functional recovery in patients with schizophrenia#br#
    LIU Dejun, CAI Jiajia, WANG Ning, ZHANG Bin,KANG Longfei,GE Jing, WANG Wei*
    2017, 37(5):  529-534.  doi:10.3969/j.issn.10073205.2017.05.008
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    〗[Abstract] Objective〖HTSS〗〓To assess the effect of art therapy on the improvement of symptoms and recovery of patients with schizophrenia.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Search involved the randomized controlled trials about the effect of art therapy on the rehabilitation of patients with schizophrenia in Cochrane Library, the JBI Database of Systematic Review, Pubmed, Wanfang, Chinese biomedical literature databases, VIP and CNKI. And the search time was Jan. 1980 to Jan. 2015. According to the inclusion and exclusion criteria, the Metaanalysis was extracted and analyzed by RevMan 5.3 software.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓A total of 9 RCTs were included. The results of Metaanalyses showed the effect of art therapy auxiliary intervention(intervention group) was more effective in negative syndromes core as compared with the standardized treatment group(control group). Art therapy was more effective in improving the level of selfesteem than the control group. However, there was no significant difference in positive syndromes core and function rehabilitation score between the art therapy intervention group and the control group in.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓On the basis of standardized treatment, art therapy intervention can improve their negative symptoms and selfesteem in the patients with schizophrenia.
    Significance and effects of reserving spinous process and posterior ligamentous complex in the procedure of laminoplasty #br#
    LI Dongfeng, LIU Fajing, HU Chengdong, HUO Xiwei, WANG Rui, LI Yanfei
    2017, 37(5):  535-538.  doi:10.3969/j.issn.10073205.2017.05.009
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    〗[Abstract] Objective〖HTSS〗〓To investigate the significance and clinical effects of reserving spinous process and rebuild posterior ligamentous complex in the procedure of laminoplasty with mini titanium plate fixation.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Eightynine cases of cervical stenosis patients were divided into 2 groups based on the operation procedure they accepted. Fortyseven patients in group A, who reserving spinous process and rebuild posterior ligamentous complex. Fortytwo patients in group B, who accepted normal laminoplasty. The Japanese  Orthopaedic Association(JOA) score, Visual Analogue Scale(VAS) score, cervical curvature index, posterior cervical muscle crosssectional area were recorded and analyzed at the point of preoperative and final followup.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓No spinal cord and nerves injury occurred during the operation. At the point of final followup, both of the 2 groups had a significant increase in JOA score and VAS score(P<005), and had a significant decrease in cervical curvature index and posterior cervical muscle crosssectional area(P<005). There was no significant difference in JOA score between the 2 groups(P>005). However, at the final followup, group B had a lower VAS score and a better cervical curvature index, posterior cervical muscle crosssectional area maintained than group A(P<005). Both of the 2 groups had no significant difference at operative time and blood loss(P>005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Laminoplasty with mini titanium plate fixation could improve the neurological function effectively for cervical stenosis patients. Moreover, if reserving spinous process and rebuild posterior ligamentous complex, a weaker axial symptom, a better cervical curvature index and a slight neck muscle atrophy will be achieved.
    Clinical effect of preemptive analgesic on early rehabilitation after tenolysis of flexor tendon adhesion
    BAI Jiangbo, ZHAO Hongfang, ZHANG Bing, YU Kunlun, MA Tao, TIAN Dehu*
    2017, 37(5):  539-541,547.  doi:10.3969/j.issn.10073205.2017.05.010
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    〗[Abstract]  Objective〖HTSS〗〓To evaluate the effect of preemptive analgesic on postoperative analgesia and early rehabilitation after tenolysis of flexor tendon adhesion.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Forty patients scheduled for tenolysis of flexor tendon adhesion were randomly divided into two groups(n=20): preemptive analgesic group and patient controlled analgesia group. Celecoxib and parecoxib were given in the preemptive analgesic group, patientcontrolled intravenous analgesia with sufentanil were adepted in the patient controlled analgesia group.Pain at rest was evaluated using visual analogue scale(VAS) score at 6, 12, 24, 48, 72 h after the operation. Total active motion(TAM) were used to assess the function of the involved digits one and two week after the operation.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The VAS score at rest between two groups was decreased, but the patient controlled analgesia group decreased slowly(P<005). TAM in the two groups was increased,but the patient controlled increased slowly(P<005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The preemptive analgesic can improve the postoperative analgesia and digits rehabilitation for the patients undergoing tenolysis of flexor tendon adhesion.
    The biomechanical changes of humeroradial joint after elbow joint medial collateral ligament injured
    ZHANG Peinan1, WU Na2, ZHANG Xin1, JIA Yongli1, YANG Xinming1, MA Pengpeng1
    2017, 37(5):  542-547.  doi:10.3969/j.issn.10073205.2017.05.011
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    〗[Abstract] Objective〖HTSS〗〓To approach the biomechanical effect of anterior funicle of medial collateralligament(MCL) on different state for the humeroradial joint by taking measurement of the vitodynamics index of humeroradial joint in different state(complete、partly brake、brake)of the MCL.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Used the human elbow joint specimen and put the pressure sensitive film into the space of humeroradial joint,then take measurement of the pressure and the contact area of humeroradial joint in the different state and the anterior funicle of MCL and in the different flex angle. Take the separation of the specimen into 3 groups according to the different state of the anterior funicle of MCL: the anterior funicle of MCL complete group(group 1), the anterior funicle of MCL injured(partly brake) group(group 2) and the anterior funicle of MCL brake group(group 3). There were 20 cases in every group. Get the data and make the statistics analysis.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared intra group, the pressure of each group at 30 ° and 60 ° is higher than that at 0 °, at 90 ° that decreased slightly, but still higher than 0 °, the difference had statistically significant(P<005). Compared between groups, the pressure of group 3 is higher than that of group 1 and group 2 at 30 ° and 60 °(P<005) but had no statistical significance at 0 ° and 90 °(P>005). Compared intra group, the contact area in every group at 30 °, 60 ° and 90 ° were less than that at 0 °, the difference had statistically significant(P<005). Compared between groups, the contact area in group 2 and group 3 were less than that in group 1 at 30 ° and 60 °(P<005) but had no statistically significant at 0 ° and 90 °(P>005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The anterior funicle of MCL had important significance in the ecstrophy stabilization of the elbow joint, and injured or break could cause the instability of interelbow joint and make increasing of the pressure and decreasing of the contact area in humeroradial joint. While the elbow joint was straighten, the pressure and the contact area in humeroradial joint was little, the elbow joint was in the stable state, it could assume more ecstrophy stress.
    Overexpression of  DNAJC6 promotes hepatocellular carcinoma#br#
    GENG Yunfeng1, LI Xiaona2, YANG Hong1, HAO Xiaohong1, HAN Shuqin1, YANG Tao1*
    2017, 37(5):  548-551.  doi:10.3969/j.issn.10073205.2017.05.012
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    [Abstract]  Objective〖HTSS〗〓To analyze the relationship between DNAJC6 expression and clinical data of patients with hepatocellular carcinoma(HCC), and to explore the effect of DNAJC6 overexpression on the clinical stages of HCC.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One hundred and two patients with hepatocellular carcinoma after operation were selected. PCR was used to detect the expression of DNAJC6〖KG*4〗mRNA in HCC tissues and analyzed with clinical data.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The DNAJC6〖KG*4〗mRNA high expression in patients with tumor diameter≤2 cm  was lower than that with tumor diameter>2 cm. The high expression rate of DNAJC6〖KG*4〗mRNA in HCC patients with stage Ⅰ to Ⅱ was lower than that in patients with stage Ⅲ. The high expression rate of DNAJC6〖KG*4〗mRNA in HCC patients with grade Ⅰ to Ⅱ was lower than that in patients with grade Ⅲ. The difference was statistically significant(P<005). There was no significant difference in the expression of DNAJC6〖KG*4〗mRNA between ages, portal vein tumor thrombus and hepatitis B virus(P>005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓DNAJC6 abnormal expression in hepatocellular carcinoma patients were with larger tumors, poor clinical stage, grade and prognosis, so DNAJC6 can be used as an important index to evaluate the preoperative condition and postoperative outcome in patients with hepatocellular carcinoma.
    Effects of biofeedback electrical stimulation combined with pelvic floor muscle training on postpartum stress urinary incontinence #br#
    HUANG Lixia, GU Yuhong, FANG Guiying, ZHANG Hongzhen*, WANG Pan, LIU Xiaoqian
    2017, 37(5):  557-560.  doi:10.3969/j.issn.10073205.2017.05.014
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    [Abstract]  Objective〖HTSS〗〓To observe the clinical effect of biofeedback electrical stimulation combined with pelvic floor muscle training in the treatment of postpartum stress urinary incontinence.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fiftynine cases of early maternal stress urinary incontinence were randomly divided into treatment group of 29 cases and control group of 30 cases. The control group was given traditional health education and pelvic floor muscle training, and the treatment group was given biofeedback electrical stimulation treatment and psychological intervention. The treatment effect of 3,6 and 12 monthes after delivery was compared.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The cure rate and effective rate of treatment group were significant higher  than that of control group(P<005) after 3,6 and 12 monthes. Pelvic floor muscle strength improvement rate of the treatment group respectively   higher than that of the control group, and the difference is statistically significant(P<005). During the treatment of 2 groups, there was no adverse effect.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓 The biofeedback electrical stimulation combined with pelvic floor muscle training can significantly improve the therapeutic effect in the treatment of postpartum stress urinary incontinence.
    Clinical observation of individualized folic acid supplementation in the prevention of neonatal diseases
    WEI Haiqing1, XU Xinlei2, WANG Fangna3, DU Jiexian4*
    2017, 37(5):  561-565.  doi:10.3969/j.issn.10073205.2017.05.015
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    [Abstract] Objective〖HTSS〗〓To investigate the effect of folic acid supplementation during pregnancy on the newborn infants with neural tube defects diseases.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Among 4 896 pregnant women who had been examined before marriage and were prepared for childbearing, a total of 1 416 pregnant women as the observation group were selected for gene testing, Using TaqmanMGB technology, genotype were performed in 5, 10methylenetetrahydrofolate reductase(MTHFR) A1298C and C677T gene loci and methionine synthase reductase(MTRR) A66G gene loci. According to the above gene function, homozygous, wild and frequency were analyzed. Ability to use folic acid gene detection was classified(normal, slightly poor, poor and very poor). Poor and very poor pregnant women were selected for individual oral folic acid supplementation and regular antenatal examination. A total of 1 416 pregnant women cases was defined as the control group, who did not receive folic acid by gene detection, only in accordance with the dose conventional oral folic acid 400 g/day and regular antenatal examination. Newborn neural tube defect disease(spina bifida and anencephaly, encephalocele, meningocele, incidence of cleft lip and palate) of 2 groups were recorded.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓①There was no significant difference in genotype and allele distribution in MTHFR two gene loci in the observation group and the Chinese population in the observation group(P>005). There was significant difference in genotype and allele distribution in. MTRR gene loci in the observation group and the Chinese population in the observation group(P<005). ②The ratio of folic acid utilization ability to detect gene was 72.46% in the observation group . ③The incidence rate of neural tube defects in pregnant women in the observation group was significantly lower than that of observation group(P<005). 
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓More than half of the folic acid utilization ability of women of childbearing age in the sample area was collected, and different folic acid supplementation scheme could effectively reduce the incidence of neural tube defects.
    Correlation analysis of influence factors in eye position return after intermittent exotropia surgery
    WANG Huan, CHAI Song*, LIU Huijie, JIANG Jiao
    2017, 37(5):  566-569,574.  doi:10.3969/j.issn.10073205.2017.05.016
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    [Abstract] Objective〖HTSS〗〓To analyze the influence factors of eye position return after surgery in patients with intermittent exotropia.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Sixtyfive patients with intermittent exotropia were treated with surgery in ophthalmology department of the second hospital of Hebei Medical University. On the first days and one week after operation, the eyes of the patients were followed up for 3-13 months, the amount of eye position was measured in 52 of 65 patients. The amount of the eye position return was measured and influence factors were analyzed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The eye position was found significant difference between the first day and one week after operation in the 65 patients(P<005). During the followup in the 52 patients, there is significant difference between the eye position at the first day and longterm eye position(P<005). The linear relationship was found in eye position between the first day and longterm. The mean amount of regression in the patients who had near stereoscopic preoperative was found more than that in the patients who had no near stereoscopic preoperative. The patients lack of collection type regressed more than that in the patients with basic type. More regression was found in esophoria group than exotropia group at first day after surgery. The differences were statistically significant(P<005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The amount of eye position return in the intermittent exotropia patients was correlated with the eye position at the first day, preoperative near stereoscopic and type of exotropia, but was not correlated with age, sex, family history, medical history, refractive status, strabismus angle, binocular visual, anesthesia, operation methods.
    The application of optical coherence tomography in the early diagnosis of primary open angle glaucoma
    DUAN Xiaoyan1, LIU Danyan2*, DUAN Jialiang2, ZHANG Bin2, LIU Xiaoli2
    2017, 37(5):  570-574.  doi:10.3969/j.issn.10073205.2017.05.017
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    [Abstract] Objective〖HTSS〗〓To study the value of optical coherence tomography(OCT) measurement of the retinal nerve fiber layer(RNFL) and the macular thickness in the early diagnosis of primary open angle glaucoma(POAG).
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓In the casecontrol study, normal eyes(30 cases, 40 eyes), ocular hypertension without risk factor(35 cases, 40 eyes), ocular hypertension with risk factor(38 cases, 40 eyes) and primary open angle glaucoma(38 cases, 40 eyes) were examined with Spectralis SDOCT to obtain the RNFL thickness and the macular thickness. All parameters were observed including superior, inferior, nasal and temporal quadrants and the mean RNFL thickness, also including superior, inferior and the total average macular thickness. Measurement results were analyzed and were compared.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓No significant difference in the RNFL and macular thickness were found between normal group and the group of ocular hypertension without risk factor(P>005). The RNFL and macular thickness in the group of ocular hypertension with risk factor was lower than that in the normal group and the group of ocular hypertension without risk factor(P<005). The RNFL and macular thickness in the POAG group was lower than that in the other three groups(P<005). 
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Optic nerve damage of early glaucoma and ocular hypertension with risk factor can be observed and quantitatively measured by optical coherence tomography. OCT has clear significance for the early diagnosis of POAG, which can provide important reference for the early treatment of ocular hypertension with risk factor.
    Experimental study of lanthanumhydroxyapatite composite coating on the osseointegration around titanium implant
    LIU Siyu1, WANG Cailing2, ZHENG Yu1, LIU Huiying1*, HOU Xiaowei1, WANG Lijie1
    2017, 37(5):  575-578,584.  doi:10.3969/j.issn.10073205.2017.05.018
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    [Abstract]  Objective〖HTSS〗〓To investigate the effect of lanthanumhydroxyapatite composite coating on osseointegration of the bone around titanium screw.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Nine New Zealand white rabbits were divided into two groups randomly(6 as in experimental grap and 3 in control group). After removing the 4 upper and lower incisors, the sockets were filled with xenogeneic freezedried bone. Then in experimental group, the titanium screw with lanthanumhydroxyapatite composite coating was implanted into socket immediately. In control group, the titanium screw with hydroxyapatite coating was taken the same procedure as in experimental group. The animals were executed respectively at 4 th, 8 th and 12 th week after operation, and the intact maxillary and mandibulary were harvested to be observed, and tested by gross observation, bone density analysis, torque test, and histological observation.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓In the two groups, the average gray values of the new bone near the titanium screw and the torque peak values of titanium screw were gradually increased with the time extension. And at the same time point of 4 th, 8 th, 12 th week after operation, the average gray values of the new bone near the titanium screw and the torque peak values of titanium screw in experimental group were higher than those of control group(P<005). The regenerated osseous tissue in experimental group shows an earlier formation and higher maturity of the lamellar bone than that in control group.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Under the condition of immediate implantation, hydroxyapatite coating and lanthanum hydroxyapatite composite coating can be used to obtain good osseointegration and bone repair of titanium screw and surrounding bone tissue. However, the lanthanum hydroxyapatite composited coating has better biological stability and biocompatibility, which can promote the osseointegration of titanium screw.
    Experimental study on conventional ultrasound and acoustic radiation force pulse imaging detecting liver damage induced by chemotherapeutic drugs #br#
    WANG Yanlin1, ZHANG Xu1, YAN Yafei1, LI Jianhui2*
    2017, 37(5):  579-584.  doi:10.3969/j.issn.10073205.2017.05.019
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    [Abstract] Objective〖HTSS〗〓To investigate ultrasonographic characteristics, acoustic radiation force impulse imaging(ARFI) values and pathological findings of rabbit liver damage after using chemotherapeutic agents.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Thirty female New Zealand rabbits were randomly divided into 3 groups, group A(control group), group B(cyclophosphamide+epirubicin group), group C(cyclophosphamide+epirubicin+fat emulsion group). The liver function and blood lipid were measured before and after treatment. The rabbit liver was examined by conventional ultrasound and acoustic radiation force imaging. After 6 cycles of chemotherapy drugs, the liver function, blood lipid, conventional ultrasound and shear wave velocity were compared before and after treatment and livers were taken out for pathological observation.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓There was no significant difference in AST and ALT before and after treatment in group A(P>005). AST and ALT in group B and group C were markedly elevated after drug use (P<005), and were higher than group A. AST and ALT in group C were higher than group B (P<005). There was no significant difference in TC before and after treatment in group A(P>005), B in group C and group TC were higher than those before treatment(P<005), the TC values of B group and C group after treatment were higher than those of A group, C group was higher than B group. There was no significant difference in TG between the A group and the B group before and after treatment(P>005), the TG in the C group was significantly higher than that before treatment(P<005), and the TG in the C group was significantly higher than that in the A group and the B group(P<005). There was no abnormality in A group after placebo. After using chemotherapy in B group, C group, liver routine ultrasound examination revealed hepatic capsular thickening, volume slightly enlarged, plump shape, different degrees of echogenic, uneven, bile duct wall and portal vein wall echogenic extrahepatic bile duct and portal vein were dilated, C group was more obvious. There was no significant difference in shear wave velocity before and after treatment in A group(P>005). The shear wave velocity of B group and C group was higher than that before treatment(P<005), and the shear wave velocity of B group and C group after treatment was higher than that of A group(P<005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓 A shortmiddle term administration of chemotherapeutic agents is not able to cause prominent fatty liver. ARFI can be utilized as a useful method for the quantitative evaluation of liver lesion caused by chemotherapeutic agents.
    Baicalein activates autophagy induced by mTOR pathway and degrades the overexpression of alphasynuclein#br#
    XIE Lixia1, ZHAO Feiyu1, WANG Mian1, YANG Chunshui2, LUO Yang3
    2017, 37(5):  585-589.  doi:10.3969/j.issn.10073205.2017.05.020
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    [Abstract]  Objective〖HTSS〗〓To investigate the effects and mechanisms of Baicalein on autophage activation and the removal of abnormal alphasynuclein aggragation in Parkinson disease. And to observe the protective effect of baicalein on mitochondrial damage induced by alphasynuclein.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The overexpression of alphasynuclein model was established in PC12 cells. Then the cells were treated by different concentrations of Baicalein. The degradation of alphasynuclein was detected by confocal laser scanning microscopy. Finally, the cell lactate dehydrogenase release was examined by use of LDH kit.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Baicalein can dosedependently reduce the expression level of alphasynuclein, activate autophagy markers LC3 and decrease the level of autophagy substrate p62. Immunofluorescence and western blotting show that Baicalein can inhibit mamamalian target of rapamycin(mTOR)〖KG*4〗to promote the formation of autophagosome. In addition, the Baicalein can decrease alphasynuclein overexpression of nerve cell toxicity by reducing the release of cell lactate dehydrogenase.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Autophagy activation induced by mTOR signal transduction pathway can lead to the removal of abnormal aggregation alphasynuclein in neurons.