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Table of Content

    25 October 2019, Volume 40 Issue 10
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    The relationship between the EEG and congition in childhood absence epilepsy#br#
    ZHANG Jing, JIN Mei, HU Jin-tong, CHEN Ling, SUN Su-zhen*
    2019, 40(10):  1123-1126.  doi:10.3969/j.issn.1007-3205.2019.10.002
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    ［Abstract］ Objective〖HTSS〗To analyze the background activity of electroencephalography(EEG) in childhood absence epilepsy and its relationship with event-related potential(ERP) P300, and to provide an objective basis for clinical evaluation of cognitive dysfunction and early intervention in childhood absence epilepsy.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗A total of 56 children(observation group) with absence of epilepsy were enrolled. And 55 healthy volunteers (control group) matched with gender, age and education level were collected. The P300 latency and amplitude of video-electroencephalogram（VEEG）and ERP were collected and retrospectively analyzed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗There was a statistically significant difference in the latency and amplitude of P300 between two groups before treatment(P＜0.05). There was no significant difference in the latency of P300 between two groups after treatment(P＞0.05). There were significant differences in the background activity of EEG between the observation group before and after treatment(P＜0.05), and the difference of P300 latency was statistically significant(P＜0.05). The background activity of EEG in the observation group was negatively correlated with the latency of P300(P＜0.05)，that is, the slower the background activity, the longer the P300 incubation period.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗Some children with typical absence epilepsy have cognitive impairment,all atypical absence children have cognitive impairment,and VEEG has a significant correlation with P300,the combination of the two can be used as an objective indicator to monitor the therapeutic effect.
    Effects of swimming training on insulin resistance and liver PPAR-gamma signaling pathway in mice #br#
    FENG Yan-jing1， LI Yan-jun2， LIU Sun-xin3*
    2019, 40(10):  1127-1131,1143.  doi:10.3969/j.issn.1007-3205.2019.10.003
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    ［Abstract］Objective〖HTSS〗To observe the effect of swimming training on liver fat content and peroxisome proliferator activated receptor-γ(PPAR-γ) signaling pathway in mice and discuss the mechanism of exercise improving insulin resistance.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗Thirty-one healthy 8-week-old male C57BL/6J mice were randomly divided into normal group(n=10), high-fat group(n=10) and exercise group(n=11). Insulin resistance animal model was established by high fat diet. The exercise group was given 12 weeks swimming training. The weight of mice was recorded before and after intervention. Fasting blood glucose(FBG) and fasting insulin(FINS) were measured. Insulin resistance index(IRI) was calculated by HOMA. PPAR-γ and pyruvate dehydrogenase kinase 4(PDK4) in liver tissue were detected by reverse transcription polymerase chain reaction(RT-PCR). The expression levels of kinase 4, PDK4 and pyruvate dehydrogenase(PDH) were observed by light and electron microscopy.
    〖HTH〗〖WTHZ〗Results〖HTSS〗After 12 weeks of intervention, the weight of the three groups was significantly higher than that before intervention, and the weight of the high-fat group was significantly higher than that of the normal group and the exercise group(P＜0.05). FINS, FBG and IRI in the high-fat group and the sports group were significantly higher than those in the normal group, while FINS, FBG and IRI in the sports group were significantly lower than those in the high-fat group(P＜0.05). HE staining showed that hepatocytes in the high-fat group presented diffuse steatosis, and the cytoplasm was filled with a large number of round lipid droplets of different sizes. Lipid droplets wrap around the nucleus or cytoplasmic side, squeezing the nucleus to one side, some in signed-ring shape. Lipid droplet formation was observed occasionally in the cytoplasm of hepatocytes of mice in the exercise group. Electron microscopy showed that a large number of lipid droplets were deposited in the hepatocytes of the high-lipid group. There were residual lipofuscin-like substances in liver cells of exercise group. The expression of PPAR-γ mRNA in the high-fat group was lower than that in the normal group, the expression of PPAR-γ mRNA in the exercise group was higher than that in the normal group, the expression of PDK4 mRNA in the high-fat group and exercise group was higher than that in the normal group, and the expression of PDH mRNA was lower than that in the normal group. The expression of PPAR-γ mRNA and PDH mRNA in the exercise group was significantly higher than that in the high-fat group, and the expression increase of PDK4 mRNA was lower than that in the high-fat group(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗Exercise can improve insulin resistance, which may be related to reducing liver steatosis in mice, up-regulating the expression of PPAR-γ mRNA in mouse liver tissue, reducing the expression of PDK4 mRNA, and increasing the expression of PDH mRNA.
    To investigate the effect of using bundled nursing mode to prevent catheter-related bloodstream infection in hemodialysis patients
    ZHOU Xiao-yan1, YANG Guo-shuai2*
    2019, 40(10):  1132-1135.  doi:10.3969/j.issn.1007-3205.2019.10.004
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    ［Abstract］Objective〖HTSS〗To investigate the effect of clustered nursing mode on prevention of catheter-related bloodstream infection in hemodialysis patients.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗A retrospective analysis of 202 patients with hemodialysis was performed. 101 patients who received routine nursing were used as the control group. 101 patients who underwent clustered nursing mode were used as observation group. The daily living ability, hospitalization time, cluster nursing satisfaction and bloodstream infections between the two groups were compared.
    〖HTH〗〖WTHZ〗Results〖HTSS〗In the observation group, the catheter indwelling time was shorter than that in the control group. The catheter-related bloodstream infection rate was lower than that of the control group. The daily living ability was significantly higher than that of the control group. The hospitalization time was significantly shorter than the control group, and the satisfaction degree and total satisfaction of the nursing model were higher than the control group, and the difference was statistically significant(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗The hemodialysis patients adopt the clustering nursing mode, which can reduce the infection rate of catheter-related bloodstream. The nursing operation is logical and targeted, which is worthy of clinical application.
    Clinical study of losartan potassium tablets combined with Bailing capsule in the treatment of chronic renal disease#br#
    YU Wen-hui1， DUAN Shu-zhong1*， YU Zai-xue2， LI Li-jing1
    2019, 40(10):  1136-1139,1148.  doi:10.3969/j.issn.1007-3205.2019.10.005
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    ［Abstract］ Objective〖HTSS〗To observe the clinical efficacy of losartan potassium tablets combined with Bailing capsules in the treatment of chronic kidney disease.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗160 patients with nephropathy were randomly divided into four groups: control group, losartan group, Bailing capsule group and combined drug group. The control group was given symptomatic treatment by controlling diet, controlling blood pressure and maintaining water, electrolyte and acid-base balance; the losartan group was given losartan potassium tablets 50 mg/time, once a day; the Bailing capsule group was given Bailing capsules 2.0 g/time, three times a day; the combined drug group was given losartan and Bailing capsules at the same time. The treatment lasted 6 months. Serum creatinine(SCr), blood urea nitrogen(BUN), 24 hour urinary protein quantification and inflammatory factor levels of leukotropin-1(Lknactin-1) and vascular endothelial growth factor(vascular endothelial growth factor) were measured before and after treatment. Vascular growth factor, high sensitive C-reactive protein(hs-CRP), tumor necrosis factor-α(TNF-α) and interleukin-18(IL-18) were detected.
    〖HTH〗〖WTHZ〗Results〖HTSS〗The overall efficacy of losartan group, Bailing capsule group and combined drug group was higher than that of control group, and the overall clinical efficacy of combined drug group was higher than that of Bailing capsule group and losartan group, with statistical significance(P＜0.05); renal function index of combined drug group was compared with that of single Bailing capsule and losartan group, after treatment, SCr, BUN After treatment, the levels of serum inflammatory factors Lkn-1, VEGF, hs-CRP, TNF-alpha and IL-18 were significantly higher in the combined group than those in the single Bailing capsule group and losartan group(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗The combination of losartan potassium tablet and Bailing capsule can reduce urinary protein, BUN and SCr content, improve renal function, relieve glomerulosclerosis, effectively delay renal fibrosis, effectively improve renal function index and inflammatory factor level.
    Clinical study on modified Kawase approach for surgical resection of petroclival meningiomas#br#
    CHEN Jian-jun， SONG Guo-zhi*
    2019, 40(10):  1140-11143.  doi:10.3969/j.issn.1007-3205.2019.10.006
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    ［Abstract］Objective〖HTSS〗To investigate the clinical value of surgical resection by modified Kawase approach in the treatment of petroclival meningiomas(PCMs).
    〖HTH〗〖WTHZ〗Methods〖HTSS〗To investigate the clinical value of surgical resection by modified Kawase approach in the treatment of PCMs.
    〖HTH〗〖WTHZ〗Results〖HTSS〗There was no significant difference between the two groups in the degree of tumor resection(P＞005).The incidence of postoperative neurological dysfunction in group A was significantly higher than that in group B(P＜0.05). There was no significant difference in postoperative quality of life between two groups(P＞005). The incidence of complications in group A was higher than that in group B(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗The surgical resection by modified Kawase approach in the treatment of PCMs has good clinical efficacy and prognosis, and has a certain safety. It can be used as the first choice for clinical treatment of patients with PCMs.
    Efficacy and prognosis of cystic wall sclerotherapy combined with arthroscopy for popliteal fossa cyst#br#
    LIU Hao， GAN Hong-quan， SUN Xiao-xin， LIANG Chun-yu， LIU Ying-jie
    2019, 40(10):  1144-1148.  doi:10.3969/j.issn.1007-3205.2019.10.007
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    ［Abstract］ Objective〖HTSS〗To explore the efficacy of cystic wall sclerotherapy combined with arthroscopic treatment of popliteal cyst and its impact on prognosis.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗Seventy cases of popliteal cyst were selected as research subjects. The patients were divided into observation group(n=38) and control group(n=32) according to the treatment method. The observation group was treated with cystic sclerosis combined with arthroscopy, and the control group was treated with arthroscope. The therapeutic effects of the two groups were observed, the Visual Angalogue Scale(VAS) scores and Lysholm scores of the two methods were evaluated. Logistic regression was used to analyze the risk factors of postoperative recurrence.
    〖HTH〗〖WTHZ〗Results〖HTSS〗After treatment, the VAS scores of the two groups showed a downward trend, the Lysholm score showed an upward trend, and the observation group showed significant changes. the total rate of observation group was significantly higher than that of the control group(P＜0.05). the recurrence rate of the observation group after 5 years was lower than that of the control group(P＜0.05), the single factor analysis and multivariate analysis showed that operative time ≥1 h, postoperative infection, and lobulated cyst were independent risk factors for postoperative recurrence(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗The long-term curative effect of cystic wall sclerotherapy combined with arthroscopy for the treatment of arthroscope is better, and the recurrence rate is lower, for patients with operation time ≥1 h, postoperative infection and lobulated cyst should be alert to postoperative recurrence.
    Closed reduction and intramedullary nailing for the treatment of elderly humeral surgical neck fracture#br#
    ZHANG Wei， GAO Tian-tian， CAI Yun-lyu， SUN Ping*， FU Shi-ping
    2019, 40(10):  1149-1153.  doi:10.3969/j.issn.1007-3205.2019.10.008
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    ［Abstract］Objective〖HTSS〗To observe the efficacy of closed reduction intramedullary nailing and locking plate internal fixation in elderly patients with humeral surgical neck fractures and their impact on shoulder joint function.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗The 86 elderly patients with fractured humeral neck fracture were divided into observation group and control group according to different internal fixation methods, each group had 43 cases. Control group was performed with open reduction and locking plate fixation, the observation group was given with closed reduction and intramedullary nailing. The clinical efficacy, complications rate, incision length, operation time, intraoperative blood loss, fracture healing time, Constant-Murley score, postoperative neck shaft angle and shoulder joint activity were compared between two groups after treatment.
    〖HTH〗〖WTHZ〗Results〖HTSS〗There was no significant difference in clinical efficacy and total excellent rate between the two groups(P＞005). The length of incision, operation time and fracture healing time in the observation group were significantly shorter compared with the control group, and the intraoperative blood loss was also significantly lower than that of the control group, and the difference was statistically significant(P＜0.05). There was no significance difference of Constant-Murley score and postoperative neck shaft angle between two groups(P＞005). There were no significant differences in the flexion, extension, abduction, adduction, external rotation and internal rotation between two groups after treatment(P＞005). There was no significant difference in the incidence of complications between the two groups(P＞005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗The efficacy of closed reduction intramedullary nailing is obvious as the same efficacy as locking plate fixation in elderly patients with humeral surgical neck fractures, with minimal invasion, less bleeding, shorter operative time and faster fracture healing.
    Expression of CIAPIN1 gene in bladder cancer and its regulation on the proliferation of T24 cells#br#
    MA Jian-feng1, LI Wei2*, LI Shou-bin3, ZHANG Chao-hua1, SHAO Li-jun1, SHA Quan1
    2019, 40(10):  1154-1157,1163.  doi:10.3969/j.issn.1007-3205.2019.10.009
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    ［Abstract］Objective〖HTSS〗To investigate the expression of cytokine-induced apoptosis inhibitor 1(CIAPIN1) gene in bladder cancer and adjacent tissues and its effect on the proliferation of bladder cancer T24 cells.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗Ninety-eight specimens of bladder cancer tissues and adjacent tissues were collected. The expression of CIAPIN1 mRNA and protein in tissue samples was detected by real-time quantitative PCR and Western blot. Knockdown of CIAPIN1 gene in bladder cancer T24 cells by siRNA.  Then MTS assay was used to detect cell proliferation.
    〖HTH〗〖WTHZ〗Results〖HTSS〗The expression of CIAPIN1 mRNA in bladder cancer tissues was significantly higher than that in adjacent tissues(P＜0.05). The expression of CIAPIN1 is independent of the patient's gender and age. However, the expression of CIAPIN1 was related to histological grade and TNM staging. The expression of CIAPIN1 in grade Ⅱ-Ⅲ bladder cancer patients was higher than that in grade Ⅰ. The expression of CIAPIN1 in TNM stage T2-T4 bladder cancer patients was higher than that in Ta-T1(P＜0.05). Western blot analysis showed that the expression of CIAPIN1 protein in bladder cancer tissues was significantly higher than that in adjacent tissues, but no changed about that of β-actin(P＜0.05). MTS results showed that knockdown of CIAPIN1 significantly inhibited the OD value of T24 cells over time compared to the control group. At 0 h, there was no difference in OD values between the control group and the knockdown group(P＞0.05). The proliferation activity of CIAPIN1 gene T24 cells was significantly lower than that of the control group at 12 h, 24 h, and 48 h(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗CIAPIN1 may promote the progression of bladder cancer by regulating cell proliferation.
    Effect of endovascular treatment of abdominal aortic aneurysm on serum T lymphocyte subsets, IL-6, RANTES and PI3K levels
    ZHANG Ji， LI Guang-ze， YU Shen-lin， BAO Ping-qian
    2019, 40(10):  1158-1163.  doi:10.3969/j.issn.1007-3205.2019.10.010
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    ［Abstract］Objective〖HTSS〗To investigate the effects of endovascular treatment of abdominal aortic aneurysm on serum T lymphocyte subsets, interleukin-6(IL-6), regulated upon activation normal T-cell expressed and secreted(RANTES) and phosphoinositide 3-kinas(PI3K) levels.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗One hundred patients with abdominal aortic aneurysm were retrospectively analyzed for clinical case data. According to different treatment methods, they were divided into control group(n=49) and observation group(n=51). The patients in the control group were treated with conventional open surgery, and the patients in the observation group were treated with endovascular aneurysm repair. Serum factors(IL-10, IL-6, hs-CRP, RANTES, TNF-α, PI3K), serum T cell subsets(CD3+, CD4+, CD8+, CD4+/CD8+), blood lipids(TC, TG, HDL-C, LDL-C) levels were compared between the two groups. The perioperative index values(intraoperative bleeding, operation time, postoperative fasting time, total hospitalization time) and postoperative complications(pulmonary complications, water and electrolyte disorders, infection, digestive system complications) were compared between the two groups.
    〖HTH〗〖WTHZ〗Results〖HTSS〗Before treatment, there was no significant difference in each index between the two groups(P＞0.05). After treatment, CD3+, CD4+, and CD4+/CD8+ in the two groups were significantly higher than those before treatment, and CD8+ were significantly lower than those before treatment. CD3+, CD4+, CD4+/CD8+ in the observation group were significantly higher than those in the control group, and CD8+ was significantly lower than that in the control group(P＜0.05). The level of IL-6, RANTES, PI3K factor in the two groups was significantly lower than that before treatment, and the level of IL-6, RANTES, PI3K factor in the observation group was significantly lower than that in the control group(P＜0.05).  The level of TC, TG, LDL-C in the two groups was lower than that before treatment, and the level of HDL-C was higher than that before treatment. The TC, TG, LDL-C in the observation group was lower than that in the control group, and the HDL-C in the observation group was higher than that in the control group(P＜0.05).  The level of hs-CRP, TNF-α in the two groups was lower than that before treatment. The level of IL-10 was higher than that before treatment, and the level of hs-CRP, TNF-α in the observation group was lower than that in the control group, and the level of IL-10 in the observation group was higher than that in the control group(P＜0.05). Those values about intraoperative and postoperative index values(intraoperative blood loss, operation time, postoperative fasting time, total hospitalization time) in the observation group were significantly better than the control group(P＜0.05).  The incidence of postoperative complications(pulmonary complications, water-electrolyte disorders, infection, digestive system complications) was significantly lower in the observation group than that in the control group(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗Endovascular treatment of abdominal aortic aneurysm can effectively improve the T lymphocyte subsets, and reduce IL-6, RANTES and PI3K factors. The effect is significant, safe and reliable.
    Comparison of individualized TSH inhibition after the surgery of papillary thyroid microcarcinoma
    ZHANG Lin-lei， JI Hong， LI Qing-huai
    2019, 40(10):  1164-1166,1171.  doi:10.3969/j.issn.1007-3205.2019.10.011
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    ［Abstract］Objective〖HTSS〗To observe the difference in recurrence/metastasis rate of papillary thyroid microcarcinoma patients under different thyroid-stimulating hormone（TSH） inhibition treatment within 5 years.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗One hundred and twenty patients with papillary thyroid microcarcinoma selected from our department were performed with surgical treatment. When the patient was confirmed to have papillary thyroid carcinoma by frozen pathology during surgery, we used total thyroidectomy and central lymph node dissection or thyroid occipital and isthmic resection and central lymph node dissection. All patients were confirmed as micropapillary carcinoma by pathological methods after the surgery. Different levels of TSH inhibition treatment were administered to the moderate-risk group and the low-risk group. Patients′ thyroids were regularly reviewed by ultrasound for about 5 years.
    〖HTH〗〖WTHZ〗Results〖HTSS〗In the moderate-risk group, patients who received TSH inhibition treatment did not differ from those who received drug substitution therapy within 5 years follow-up(P＞0.05). In the low-risk group, there also was no significant difference between the TSH inhibition treatment and drug substitution therapy(P＞0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗For the moderate-risk and low-risk groups of papillary thyroid microcarcinoma, drug substitution did not increase the recurrence rate after the operation within 5 years.
    Distribution of pathogenic bacteria and its risk factors in patients with cervical cancer secondary vaginitis
    LIU Hai-xia1, SI Bai-hui1, WAN Hui1, DU Jian-wen2
    2019, 40(10):  1167-1171.  doi:10.3969/j.issn.1007-3205.2019.10.012
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    ［Abstract］Objective〖HTSS〗To investigate the distribution of pathogenic bacteria in patients with secondary vaginosis of cervical cancer and to analyze its risk factors.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗The clinical data of 120 patients with cervical cancer were analyzed retrospectively. The patients with secondary vaginitis were recorded as the occurrence group, otherwise recorded as the non-occurrence group, the constituent ratio of the factors that may affect the secondary vaginitis of cervical cancer in the two groups were compared, and the possible influencing factors were analyzed by Logistic regression analysis.
    〖HTH〗〖WTHZ〗Results〖HTSS〗Among the 120 patients with cervical cancer, 42(35.00%) were secondary vaginosis. A total of 72 strains of pathogenic bacteria were detected in patients with secondary vaginitis. The proportion of fungi, Gram-negative bacteria and Gram-positive bacteria were 52.78%, 31.94% and 15.28%, respectively. Among them, Candida albicans, Escherichia coli and Staphylococcus accounted for the highest proportion, which were 27.78%, 12.50% and 8.33%, respectively. The age >60 years old, past history of vaginitis, diabetes mellitus, postoperative drainage time > 24 hours, chemotherapy and radiotherapy in the occurrence group were higher than those in the non-occurrence group, and the constituent ratio of vaginal lotion was lower than that in the non-occurrence group. The difference was statistically significant(P < 0.05). The results of Logistic regression analysis showed that age >60 years old, past history of vaginosis, diabetes mellitus, postoperative drainage time >24 hours, chemotherapy and radiotherapy were the risk factors of secondary vaginitis of cervical cancer. Vaginal lotion was a protective factor for secondary vaginosis of cervical cancer(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗The risk of secondary vaginitis of cervical cancer is high. Fungi is the main pathogen and there are many risk factors. It is necessary to formulate prevention and control measures according to the risk factors and encourage patients to use vaginal lotion reasonably.
    Application of growth hormone in older patients with mild-stimulus protocol#br#
    ZHAO Zhen-chuan, QI Rong, LIANG Ying, WANG Juan, GAO Xing
    2019, 40(10):  1172-1176.  doi:10.3969/j.issn.1007-3205.2019.10.013
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    ［Abstract］ Objective〖HTSS〗To investigate the therapeutic effect of growth hormone (GH) on ovulation induction in elderly patients with micro-stimulation.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗A retrospective analysis of 180 patients with in vitro fertilization and embryo transfer(IVF-ET), all using micro-stimulation,according to whether GH was added or not,90 cases were divided into study group(GH added) and control group(GH not added) 90 cases.Analyze and compare the general situation of the two groups, IVF related indicators and assisted pregnancy outcomes.
    〖HTH〗〖WTHZ〗Results〖HTSS〗①There was no significant difference in the age, infertility years and basal endocrine levels between two groups(P＞0.05). ②There were no significant differences in total gonadotropin, days of Gn use, estrogen level on human chorionic gonadotropin(HCG) day, endometrial thickness on HCG day, transplantation number and cycle cancellation rate between two groups(P＞0.05). ③The number of eggs harvested, fertilization rate， cleavage rateand number of high-quality embryos in the study group were significantly higher than those in the control group(P＜0.05). ④The pregnancy rate in the study group was slightly higher than that in the control group, but there was no significant difference(P＞0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗GH can increase the number of eggs harvested and fertilization rate, cleavage rate， improve the quality of embryos and help to improve the clinical pregnancy rate when microstimulus is used in elderly patients.
    The impact of systematic health education on breast cancer screening#br#
    WANG Ying1, PAN Dong-chen2, CUI Shi-jie3, LIU Wen-cong4, CHANG Jiang5, ZHANG Peng5*
    2019, 40(10):  1177-1179,1184.  doi:10.3969/j.issn.1007-3205.2019.10.014
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    ［Abstract］Objective〖HTSS〗To explore the effect of systematic health education on breast cancer screening.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗According to inclusion criteria, 356 women were randomly divided into health education group and control group, comprehensive systematic health education was adopted in the health education group, and routine health guidance was adopted in the control group, then  the cognitive and screening rate of breast cancer in the two groups were compared.
    〖HTH〗〖WTHZ〗Results〖HTSS〗After systematic health education, the cognitive of breast cancer, including etiology and high-risk factors, early/main clinical manifestations, treatment methods, prevention knowledge, main screening methods and other aspects in the health education group were significantly higher than those in the control group and before intervention(P＜0.05). The positive scores and satisfaction scores of health education in the health education group were higher than those in the control group, and the screening rate of breast cancer in the health education group was higher than that in the control group(P＜0.05). 
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗Systematic health education is beneficial to improve women′s cognition of breast cancer, their enthusiasm to participate in screening and their satisfaction with systematic health education.
    Allergens seasonal difference analysis on 3 236 patients with allergic diseases in Beijing
    CHEN Xiang-mei, ZHANG Liang, QI Li-ming, MA Zhi-yuan, WANG Peng*
    2019, 40(10):  1180-1184.  doi:10.3969/j.issn.1007-3205.2019.10.015
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    ［Abstract］ Objective〖HTSS〗To explore the population difference and allergen distribution of allergen detection patients in different seasons in Beijing.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗Immunoblotting was used to detect 19 allergen specific IgE antibodies in 3 236 patients′ serum. The differences of number of people, sex, age and allergen distribution in different seasons were analyzed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗There are 1 273 males(spring 199/summer 250/autumn 492/winter 332) and 1963 females(spring 278/summer 372/autumn 645/winter 668) among 3236 patients. There were significant differences in the number composition of allergens between different sexes in different seasons(χ2=24.485, P=0.000). The number of patients in autumn and winter was more than other seasons. There were significant differences in the number of patients of different age groups in different seasons(χ2=40.025, P=0.000), and the number of young patients was higher. The top five allergen positive rates were Artemisia argyi(11.12%), dust mite combination(9.39%), cat hair(6.61%), sea fish combination(5.81%) and crab(4.67%). The top three allergen positive rates in spring were dust mite combination/sea fish combination/Artemisia argyi(10.06%/6.92%/6.71%)， the top three in summer were dust mite combination/Artemisia argyi/sea fish combination(12.54%/10.29%/5.14%), the top three in autumn were Artemisia argyi/cat hair/dust mite combination(15.83%/8.44%/8.18%), and the top three in winter were dust mite combination/Artemisia argyi/cat hair(8.80%/8.40%/6.64%). There were significant differences in the distribution of allergens in different seasons(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗In autumn and winter, the population of allergic diseases in Beijing increased sharply, mainly caused by inhalation allergens. Artemisia argyi, dust mites and cat hair were the main allergens, while marine fish and crab were the main food allergens, showing obvious seasonal differences.
    A study towards the effect of orthodontic treatment on the changes of gingival protein R and the risk of gingival inflammation#br#
    WU Yang-xuan1, CHU Jin-hai1, LIU Qiu-hong2
    2019, 40(10):  1185-1188.  doi:10.3969/j.issn.1007-3205.2019.10.016
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    ［Abstract］ Objective〖HTSS〗To investigate the effect of orthodontic treatment on the changes of gingival protein R(Rgp) and to analyze the risk of gingivitis.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗A total of 118 adolescent patients who underwent orthodontic treatment were randomly selected. The gingival crevicular fluid was taken before, after, and at 1, 2, 3, and 6 months after correction. The 16S rDNA PCR technique was used to detect Porphyromonas gingivalis（P.g） and Rgp. Chi-square test was used to compare the detection rates of P.g and Rgp between groups, and Spearman rank correlation analysis was used to analyze the relationship between gingivitis and P.g and Rgp detection rates.
    〖HTH〗〖WTHZ〗Results〖HTSS〗Among the 118 subjects, the positive rate of P.g detection before, after, 1, 2, 3, and 6 months after correction was 29.66%, 53.39%, 72.03%, 60.17%, 38.14%, compared with the pre-correction, after correction. The positive rate of P.g detection increased gradually in the first month and 2 months, and decreased in the third month(P＜0.05). The positive rate of Rgp in the positive detection of P.g before and after correction, 1, 2, 3, and 6 months after correction were 34.29%, 53.97%, 69.41%, 67.61%, 42.22%. Compared with the pre-correction, the positive rate of Rgp detection increased gradually after 2 and 3 months of correction(P＜0.05). There was a positive correlation between gingival index and detection time, P.g. and positive rate of Rgp(rs=0.352， 0.461， P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗In the early stage of orthodontic treatment, the appliance can increase P.g and Rgp, and begin to decline in the third month. The sixth month is slightly higher than before correction, and oral hygiene should be emphasized during orthodontic treatment, which is conducive to the prevention of gingivitis.
    Effect of ALA-PDT combined with tetracaine hydrochloride mucilage on the treatment of condylomata acuminata and influence of serum factors#br#
    MA Mei1， CAO Fang2， WANG Ming-ming2*
    2019, 40(10):  1189-1192,1196.  doi:10.3969/j.issn.1007-3205.2019.10.017
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    ［Abstract］ Objective〖HTSS〗To explore effect of 5-aminolevulinic acid photodynamic treatment(ALA-PDT) combined with tetracaine hydrochloride mucilage on the treatment of condylomata acuminata and influence of serum of lymphotactin(LTN), tumor necrosis factor α(TNF-α) and substance P(SP).
    〖HTH〗〖WTHZ〗Methods〖HTSS〗Eighty-two cases of condylomata acuminata patients were equally divided into control group and observation group by the digital random table. Patients in the control group were treated with ALA-PDT, patients in the observation group were treated with ALA-PDT combined with tetracaine hydrochloride mucilage. The clinical effect, recurrence, adverse reactions and the serum level of LTN, TNF-α, SP of two greoups were compared.
    〖HTH〗〖WTHZ〗Results〖HTSS〗The clinical efficacy and total effective rate of the observation group were higher than those of the control group(P＜0.05). The recurrence rate and the average number of recurrence warts in the observation group were significantly lower than those in the control group(P＜0.05). The serum LTN in the control group and the observation group after treatment was significantly higher than that before treatment(P＜0.05), and the serum TNF-a and SP in the two groups were significantly lower than those before treatment(P＜0.05). The serum LTN in the observation group was significantly higher than that in the The incidence of adverse reactions in the observation group was significantly lower than that in the control group(P＜0.05), while the incidence of TNF-α and SP in the observation group was significantly lower than that in the control group(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗ALA-PDT combined with tetracaine hydrochloride gel has excellent curative effect on condyloma acuminatum, low recurrence rate and less adverse reactions. It can regulate local cellular immunity by increasing LTN level and decreasing TNF-alpha and SP level, which is worthy of clinical reference and application.
    A mechanism study about the effect of oxidizedcellulose absorbable hemostatic gauze on skin wound healing in rats#br#
    CUI Zhi-hui1， ZHANG Ya-fei2， TIAN Tian2， DONG Xiao-hui3， JIA Qing-zhong1,3， ZHANG Lian-shan3*
    2019, 40(10):  1193-1196.  doi:10.3969/j.issn.1007-3205.2019.10.018
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    ［Abstract］ Objective〖HTSS〗To assess the effects of oxidizedcellulose absorbable hemostatic gauze(OAHG) to the course of ctaneous wound.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗A total of 24 Wistar rats were used. Three full-thickness excisional wounds were made on each side of the dorsal midline with a 15 mm punch biopsy in one rat. Two wounds were covered with OAHG and surgicel and one wound was used as blank control studies(no gauze). Wound-closure kinetics were observed.  Histological sections of day4, 7 , 14 and 21 wounds were analyzed，Expression of α-smooth muscle actin(α-SMA) within the granulation tissue and collagen formation were evaluated.
    〖HTH〗〖WTHZ〗Results〖HTSS〗Course of the wound-closure kinetics were not significantly different in three groups： Histological analysis showed that a dehydrated crust, granulation tissue and collagen formmationin in three groups，granulation tissues were formed at margeing of excisional wounds by day 4, peaked at day 7.  Formation of the collagen increased at day 14 and epithelial cells coveried all wound in three groups at day 21. Expressive patterns of α-SMA were similar to the three groups but the peaks of α-SMA expression in both gauze groups were lower and delayer than in black control.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗OAHG does not affect the healing of skin wounds in rats, and there is no significant difference between domestic and foreign similar products(instant gauze).
    Effects of dexmedetomidine combined with oxycodone on perioperative extubation stress response in patients with nasal endoscopic surgery#br#
    FANG Ming-xing, ZHANG Qi
    2019, 40(10):  1197-1200,1206.  doi:10.3969/j.issn.1007-3205.2019.10.019
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    ［Abstract］Objective〖HTSS〗To observe the effects of dexmedetomidine combined with oxycodone on perioperative extubation stress response in patients with nasal endoscopic surgery.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗Sixty patients who underwent selective nasal endoscopic surgery were randomly divided into sufentanil group and oxycodone group, with 30 cases in each group. Sufentanil group was given 0.4 μg/kg sufentanil and 0.5-0.6 μg/kg dexmedetomidine for anesthesia induction, and oxycodone group was given 0.2 mg/kg oxycodone and 0.5-0.6 μg/kg dexmedetomidine for anesthesia induction. Mean arterial pressure(MAP), heart rate(HR), cortisol(COR) and blood glucose(GLU) were recorded before anesthesia induction(T0), at 1 min after tracheal intubation(T1), at the beginning of surgery(T2), at extubation(T3) and at 10 min after extubation(T4). The wake-up time, extubation time, extubation quality score, Ramsay sedation score and occurrence of adverse reactions were compared between two groups.
    〖HTH〗〖WTHZ〗Results〖HTSS〗The MAP, HR, COR and GLU in two groups were increased first and then decreased, and the changes of MAP, HR, COR and GLU in oxycodone group were significantly lower than those in sufentanil group, and there were statistically significant differences between-group effects, time-point effects and interaction effects of between-group and time-point(P＜0.05). The extubation quality score in oxycodone group was significantly lower than that in sufentanil group while the Ramsay sedation score was significantly higher than that in sufentanil group, and the incidence rate of adverse reactions was lower than that in sufentanil group(P＜0.05). There were no significant differences in wake-up time and extubation time between two groups(P＞0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗Dexmedetomidine combined with oxycodone anesthesia induction for nasal endoscopic surgery can effectively maintain hemodynamic stability, inhibit perioperative extubation stress response, and reduce the incidence rate of adverse reactions.
    Effects of dexmedetomidine on perioperative stress response in patients undergoing gynecological laparoscopic surgery at different ages#br#
    LI Hong, WU Jun-na, LIU Ye, LI Teng, ZHANG Jin*
    2019, 40(10):  1201-1206.  doi:10.3969/j.issn.1007-3205.2019.10.020
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    ［Abstract］ Objective〖HTSS〗To evaluate the effect of dexmedetomidine on perioperative anesthesia in patients undergoing gynecological laparoscopic surgery of different ages.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗A total of 105 patients, weighing 55-75 kg, of American Society of Anesthesiologists physical status Ⅰ or Ⅱ, undergoing elective gynecologic laparoscopic surgery under general anesthesia, were divided into 3 groups(n=35 each)， according to age: youth group(18-44 years old), middle-aged group(45-64 years old) and elderly group(65-75 years). All patients were intravenously injected with dexmedetomidine 0.5 μg·kg-1·h-1 10 min before anesthesia induction, and then continuously pumped with 0.5 μg·kg-1·h-1 until the end of surgery. The mean arterial pressure(MAP) and heart rate(HR) were recorded at the time of admission(T0), intubation(T1), intubation 1 min(T2), intubation 5 min(T3), extubation(T4), extubation 10 min(T5), extubation 1 h(T6), and blood glucose and serum cortisol concentrations were measured at the corresponding time points. The dosage of propofol and remifentanil, respiratory recovery time, recovery time, extubation time, Ramsay score, Riker sedation/agitation score(SAS) and adverse reactions were recorded.
    〖HTH〗〖WTHZ〗Results〖HTSS〗With the extension of time, MAP and HR in the three groups decreased first, then increased and then decreased. Blood glucose in the young and middle-aged groups increased first, then increased and then decreased. Blood glucose in the elderly group increased first and then decreased. Serum cortisol concentrations increased first and then decreased gradually, and the elderly group was lower than the youth group and the middle-aged group at all time points(P＜0.05). There were significant differences in interaction among the three groups, time points and time points between the groups(P＜0.05), and the propofol and fentanyl dosage reduced, Ramsay score increases, and the incidence of adverse reactions were also increased significantly in elderly group(P＜0.05). There was no statistical significance for each index in the young and middle-aged group(P＞0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗Dexmedetomidine can inhibit perioperative stress response of gynecological laparoscopic surgery patients of different ages, increase sedative effect, and there are age differences. The inhibition of stress response in elderly patients is more obvious, but adverse reactions also increase. The dosage of dexmedetomidine should be adjusted according to patient′s age in the course of use.
    Evaluation of analytical performance routine chemistry projects in 26 result mutual recognition laboratories in Hebei province by σ metric method#br#
    WU Hao， WANG Hui-ru*， LI Gui-xia， LIU Bin， WANG Sheng-hua， FENG Zhi-shan
    2019, 40(10):  1207-1210,1225.  doi:10.3969/j.issn.1007-3205.2019.10.021
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    ［Abstract］Objective〖HTSS〗To measure and analyze the detection performance of clinical biochemical items in 26 mutual recognition laboratories in Hebei province by using the Sigma value(σ), to understand their quality level, and to propose improvement plans.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗Inter-room comparison data of internal quality control(IQC) were collected from 26 laboratories in Hebei province, including alanine aminotransferase(ALT), aspartate aminotransferase(AST), Na+, K+, total cholesterol(TC) and triglyceride(TG) were used as total error allowance(TEa) to evaluate the quality status of clinical biochemical routine items.
    〖HTH〗〖WTHZ〗Results〖HTSS〗Twenty-six laboratories with quality control L1 item performance(≥6σ) accounted for ALT 3.85%, AST 3.85%, Na+ 3.85%, K+ 23.08%, TC 19.23% and TG 19.23%, and laboratories with quality control L2 item performance(≥6σ) accounted for ALT 46.15%, AST 50.00%, Na+ 3.85%, K+ 26.92%, TC 3.85% and TG 7.69%. Laboratories with performance 3≤σ＜6 at L1 of quality control accounted for ALT 73.08%, AST 76.92%, Na+ 96.55% , K+ 77.05%, TC 73.08% and TG 77.05%. Labs with performance 3≤σ＜6 at L2 of quality control accounted for ALT 53.85%, AST 50.00%, Na+ 92.31%, K+ 73.08%, TC 69.23% and TG 77.05%. 
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗The quality level of 26 laboratories in Hebei province has excellent performance. 6σ provides a unified and simple evaluation criterion, which is more convenient and intuitive for IQC comparisons.