河北医科大学学报 ›› 2020, Vol. 41 ›› Issue (10): 1154-1159.doi: 10.3969/j.issn.1007-3205.2020.10.009

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rsa基因通过mbk-2调控秀丽隐杆线虫胚胎发育

  

  1. 1.河北医科大学第二医院生殖医学科，河北 石家庄 050000；2.河北省廊坊市人民医院妇产科，河北 廊坊 065000；
    3.河北医科大学基础医学院药理学教研室，河北 石家庄 050017；4.河北医科大学基础医学院
    组织胚胎学教研室，河北 石家庄 050017
  • 出版日期:2020-10-25 发布日期:2020-10-27
  • 作者简介:翟佳佳（1986-），女，河北衡水人，河北医科大学第二院主治医师，医学博士研究生，从事生殖医学研究。
  • 基金资助:
    国家自然科学基金资助项目（81770407）；河北省自然科学基金资助项目（C2018206277）

RSA gene regulates embryonic development of Caenorhabditis elegans by mbk-2

  1. 1.Department of Reproductive Medicine, the Second Hospital of Hebei Medical University, Shijiazhuang 
    050000, China; 2.Department of Gynecology and Obstetrics, the People′s Hospital of Langfang, 
    Hebei Province, Langfang 065000, China; 3.Department of Pharmacology, the School of 
    Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China; 4.Department of 
    Histology and Embryology, the School of Basic Medicine, Hebei Medical University, 
    Shijiazhuang 050017, China
  • Online:2020-10-25 Published:2020-10-27

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摘要： 目的  使用秀丽隐杆线虫作为模式生物，观察rsa基因对mbk-2突变型线虫胚胎发育和虫卵孵化的影响，初步探讨mbk-2的在胚胎发育中的作用及机制。
方法  以RNAi技术定向干扰mbk-2突变型线虫rsa基因的表达，并对rsa沉默后线虫的虫卵孵化水平及胚胎发育情况进行分析，检测其在线虫生长发育、生殖能力等方面的变化。
结果  与L4440喂养的mbk-2基因突变组相比，rsa-1和rsa-2RNAi细菌处理mbk-2基因突变组虫卵未孵化率显著升高，达到41.8%和49.2%（P＜0.05），且rsa-2处理组显著高于rsa-1组（P＜0.05）；rsa-1和rsa-2 RNAi细菌处理的N2野生型线虫虫卵未孵化率分别为3.9%和5.7%，显著低于rsa-1和rsa-2 RNAi细菌处理的mbk-2突变型线虫（P＜0.05）。 rsa-2 RNAi处理mbk-2突变型线虫组，线虫胚胎发育异常显著增加。
结论  mbk-2是线虫发育的一个重要因子，rsa是mbk-2的重要修饰基因，两者相互作用调节线虫的胚胎发育和虫卵孵化。


关键词: 胚胎和胎儿发育, 线虫纲, 基因

Abstract: Objective  To observe the effects of rsa gene on embryonic development and egg hatching of mbk-2 mutant C. elegans by using Caenorhabditis elegans(C.elegans) as a model organism, and the role and mechanism of mbk-2 in embryonic development were preliminarily discussed. 
Methods  RNAi technology was used to directionally interfere with the expression of rsa gene in mbk-2 mutant C. elegans. The egg hatching level and embryonic development of nematode after rsa silencing were analyzed to detect the changes in the growth and reproductive capacity of C. elegans. 
Results  Compared with mbk-2 gene mutation group fed with L4440, the hatchability of eggs in mbk-2 gene mutation group treated with rsa-1 and rsa-2 RNAi bacteria increased significantly, reaching 41.8% and 49.2%(P＜0.05), and rsa-2 treatment group was significantly higher than rsa-1 group(P＜0.05). The hatchability of eggs of N2 wild-type C. elegans treated by rsa-1 and rsa-2 RNAi bacteria was 3.9% and 5.7% respectively, which was significantly lower than that of mbk-2 mutant C. elegans treated by rsa-1 and rsa-2 RNAi bacteria(P＜0.05). rsa-2 RNAi treatment of mbk-2 mutant C. elegans significantly increased abnormal embryonic development. 
Conclusion  mbk-2 is an important factor in C. elegans development, rsa is an important modification gene of mbk-2, and the two interact to regulate C. elegans embryo development and egg hatching.


Key words: embryonic and fetal development, nematoda, genes

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