乙型肝炎儿童T细胞亚群与病毒复制及病毒载量关系的研究

doi:10.3969/j.issn.10073205.2018.02.019

河北医科大学学报

乙型肝炎儿童T细胞亚群与病毒复制及病毒载量关系的研究

河北钢铁集团宣钢职工医院检验科，河北张家口 075100

出版日期:2018-02-25 发布日期:2018-02-06
作者简介:张俊婷（1979-），女，河北张家口人，河北钢铁集团宣钢职工医院主管检验师，医学学士，从事临床检验学研究。
基金资助:
张家口市科技攻关计划项目(1621032D)

Study on T lymphocyte subsets and viral replication and viral load in children with hepatitis B

Department of Clinical Laboratory， Xuangang Worker′s Hospital of Hebei Iron and Steel Group, Zhangjiakou 075100， China

Online:2018-02-25 Published:2018-02-06

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗探讨乙型肝炎患儿T细胞亚群与病毒复制及病毒载量的关系。
〖HTH〗方法〖HTSS〗〖KG*2〗选取乙型肝炎病毒脱氧核糖核酸（hepatitis B virusdesoxyribose nucleic acid，HBVDNA）阳性的慢性乙型肝炎患儿58例为研究对象，其中乙型肝炎E抗原（human hepatitis B virus e antigen，HBeAg）阳性41例、HBeAg阴性17例，低载量患儿24例、中载量患儿19例、高载量患儿15例；另选取同期健康体检儿童35例为对照组。采用荧光定量PCR法检测HBVDNA，应用流式细胞仪测定CD3+、CD4+、CD8+，计算CD4+/CD8+。
〖HTH〗结果〖HTSS〗〖KG*2〗慢性乙型肝炎患儿CD3+、CD4+、CD4+/CD8+低于对照组，CD8+高于对照组（P＜001）。中载量组CD3+、CD4+、CD4+/CD8+低于低载量组，高载量组CD3+、CD4+、CD4+/CD8+低于低载量组和中载量组（P＜001）；中载量组和高载量组CD8+高于低载量组（P＜001）。HBeAg阳性患儿CD3+、CD4+、CD4+/CD8+均低于HBeAg阴性患儿，CD8+高于HBeAg阴性患儿（P<0.01）。
〖HTH〗结论〖HTSS〗〖KG*2〗乙型肝炎患儿T细胞亚群的改变不仅与HBV感染有关，而且还与HBVDNA复制有关，其复制可进一步加重细胞免疫功能紊乱。

关键词: 乙型肝炎, T淋巴细胞亚群, 病毒复制, 病毒载量

Abstract: ［Abstract］〓Objective〖HTSS〗〓To explore the correlation of T lymphocytes with virus replication and viral load in children with hepatitis B.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fiftyeight children with chronic hepatitis B, who were positive for hepatitis B virusdesoxyribose nucleic acid(HBVDNA), were selected as the research subjects. Among them, 41 had positive human hepatitis B virus e antigen（HBeAg）, 17 had negative HBeAg, 24 had low viral load, 19 had moderate viral load, and 15 had high viral load. Moreover, 35 healthy children were served as the control group. FQPCR was used to detect HBVDNA. FCM was used to detect CD3+, CD4+, and CD8+. CD4+/CD8+ were calculated.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓CD3+, CD4+, CD4+/CD8+ in children with Chronic hepatitis B were lower than the control group, CD8+ was higher than the control group (P＜001). CD3+, CD4+, CD4+/CD8+ in load group were lower than that of low load group, CD3+, CD4+, CD4+/CD8+in higher load group were lower than that of low load group and load group(P＜001), load group and high load of CD8+ was higher than that of low load group(P＜001). The CD3+, CD4+ and CD4+/CD8+ of HBeAg positive children were lower than those of HBeAg negative children, and CD8+ was higher than that of HBeAg negative children(P＜001).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Changes of T lymphocyte subsets in children with hepatitis B was not only associated with HBV infection, but also correlated with the replication of HBVDNA, which can further aggravate the cellular immune dysfunction.

Key words: hepatitis B, Tlymphocyte subsets, virus replication, viral load

张俊婷，安和兵. 乙型肝炎儿童T细胞亚群与病毒复制及病毒载量关系的研究[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2018.02.019.

ZHANG Junting, AN Hebing. Study on T lymphocyte subsets and viral replication and viral load in children with hepatitis B[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2018.02.019.

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乙型肝炎儿童T细胞亚群与病毒复制及病毒载量关系的研究

Study on T lymphocyte subsets and viral replication and viral load in children with hepatitis B

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