骨形态发生蛋白9对人卵巢黄素化颗粒细胞雌、孕激素分泌功能的影响

doi:10.3969/j.issn.10073205.2018.07.014

河北医科大学学报

骨形态发生蛋白9对人卵巢黄素化颗粒细胞雌、孕激素分泌功能的影响

1.河北省保定第七医院生殖医学科，河北保定 072150；2.河北医科大学第四医院生殖医学科，河北石家庄 050011

出版日期:2018-07-25 发布日期:2018-07-04
作者简介:赵爱娟（1971-），女，河北保定人，河北保定第七医院副主任医师，医学学士，从事辅助生殖技术研究。
基金资助:
河北省科技计划项目(16277774D)

The effects of bone morphogenetic protein9 on estradiol and progesterone production in human luteinized granulosa cells in vitro#br#

1.Department of Reproductive Medical Center, Baoding Seventh Hospital, Hebei Province, Baoding
072150, China; 2.Department of Reproductive Medical Center, the Forth Hospital of
Hebei Medical University, Shijiazhuang 050011, China

Online:2018-07-25 Published:2018-07-04

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗探讨骨形态发生蛋白9（bone morphogenetic protein9，BMP9）对人卵巢黄素化颗粒细胞雌激素(estradiol，E2)和孕激素(progesterone，P)分泌功能的影响。
〖HTH〗方法〖HTSS〗〖KG*2〗收集因输卵管因素行体外受精-胚胎移植(in vitro fertilization embryo transfer,IVFET) 患者的颗粒细胞。将颗粒细胞在不同的浓度BMP9(0、3、6、12 μg/L)单独及与卵泡刺激素（folliclestimulating hormone,FSH）75 U/L共培养72 h，采用酶联免疫吸附测定法检测颗粒细胞E2、P的浓度，Westernblot检测颗粒细胞中E2生成相关因子芳香酶化P450（aromatase P450，P450 arom）、P生成相关因子类固醇急性调节蛋白（steroidogenic acute regulatory protein，StAR）的表达水平。
〖HTH〗结果〖HTSS〗〖KG*2〗单独BMP9培养时，BMP9各浓度组分泌的E2和P浓度变化不大，差异均无统计学意义（P＞005）；加入FSH后，BMP9各浓度组分泌的E2变化不大，而P浓度则随着BMP9浓度的增加，出现下降趋势，各浓度组间差异均有统计学意义（P＜005）。单独BMP9培养时，P450 arom蛋白、StAR蛋白表达水平在BMP9各浓度组变化不大，各组间差异无统计学意义（P＞005)；加入FSH后，BMP9各浓度组分泌的P450 arom蛋白变化不大，而StAR蛋白浓度则随着BMP9浓度的增加，出现下降趋势，各浓度组间差异均有统计学意义（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗BMP9在缺少FSH刺激下对颗粒细胞分泌E2、P无明显影响；BMP9可抑制FSH诱导的P的生物合成，对E2无明显影响；BMP9可作为黄素化抑制剂，防止卵泡提前黄素化，并调控卵泡的发育。

关键词: 骨形态发生蛋白9, 卵泡刺激素, 雌二醇, 孕酮

Abstract: ［Abstract］ Objective〖HTSS〗〓To investigate the effect of bone morphogenetic protein9(BMP9) on the secretion of estrogen(E2) and progesterone(P) in human ovarian luteinized granulosa cells.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Collect granulosa cells in patients undergoing in vitro fertilization embryo transfer(IVFET). Human ovarian luteinized granulosa were cultured with medium plus variousc oncentration of BMP9(0, 3, 6, 12 μg/L)and folliclestimulating hormone（FSH） 75 U/L or alone for 72 h. The media were collected to measure the concentration of E2 and P by enzyme linked immunosorbent assay. And Westernblot method was used to determine the relative expression of aromatase P450(P450 arom) and steroidogenic acute regulatory protein(StAR) proteins.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Addition of different concentrations of BMP9 alone had no significant effect on E2 and P production by luteinized granulosa cells. Different concentrations of BMP9 cocultured with FSH had no significant effect on the secretion of E2, but the secretion of P was decreased with the increase of BMP9 concentration. Addition of different concentrations of BMP9 alone had no significant effect on P450 arom and StAR protein production by luteinized granulosa cells. Different concentrations of BMP9 cocultured with FSH had no significant effect on the expression level of P450 arom protein,but the expression level of StAR protein was decreased with the increase of BMP9 concentration.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓BMP9 had no significant effect on granulosa cells secreting E2 and P in the absence of FSH stimulation. BMP9 inhibited FSHinduced P biosynthesis and had no significant effect on E2. BMP9 may act as a luteinizing inhibitor and prevent follicles from advancing, and regulate the development of follicles.

Key words: bone morphogenetic protein 9, folicle stimulating hormone, estradiol, progesterone

赵爱娟1，刘〓灿2，汪〓辉2，任泽亚2，甄秀丽2*. 骨形态发生蛋白9对人卵巢黄素化颗粒细胞雌、孕激素分泌功能的影响[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2018.07.014.

ZHAO Aijuan1, LIU Can2, WANG Hui2, REN Zeya2, ZHEN Xiuli2*. The effects of bone morphogenetic protein9 on estradiol and progesterone production in human luteinized granulosa cells in vitro#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2018.07.014.

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骨形态发生蛋白9对人卵巢黄素化颗粒细胞雌、孕激素分泌功能的影响

The effects of bone morphogenetic protein9 on estradiol and progesterone production in human luteinized granulosa cells in vitro#br#

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