关键词: 阿尔茨海默病, RNA, 未翻译, RNA, 信使

Abstract: Objective To investigate the correlation of non-coding RNA(ncRNA) dysfunction in plasma of patients with Alzheimer′s disease(AD) at the stage of amnestic mild cognitive impairment(aMCI) and the pathophysiology of AD and to explore the potential biomarkers and mechanisms underlying AD. 
Methods Flve subjects with aMCI who met the golden standard for diagnosis and five normal controls(NC) were enrolled. Microarray analysis technology was used to analyze the expression profiles of long noncoding RNA(lncRNA), microRNA(miRNA) and messenger RNA(mRNA) in plasma of aMCI and NC. R software was used to screen out differentially expressed lncRNA, miRNA and mRNA, and PicTar 2005, TargetScan 5.1 and miRanda v5 database were used to detect the relationship between RNAs, to establish a competitive endogenous RNA(ceRNA) network. Gene Ontology(GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were conducted for the differentially expressed mRNAs. 
Results A total of 287 lncRNAs, 46 miRNAs and 208 mRNAs were differentially expressed in the peripheral plasma between aMCI and NC(P＜0.05), in which 3 lncRNAs, 5 miRNAs and 7 mRNAs participated in the construction of ceRNA regulatory network. The results of GO and KEGG showed that differentially expressed mRNAs were mainly accumulated in biological processes such as RNA metabolism, cytoplasmic stress granules, transcriptional regulatory factor activities, herpes simplex infection pathway, oxytocin signaling pathway, and folic acid synthesis pathway. 
Conclusion Different ncRNAs are involved in the pathogenesis of AD. The constructed ceRNA network helps to improve the understanding of the molecular biology of AD, and ncRNAs may become biomarkers for AD diagnosis and new targets for therapeutic intervention.

Key words: Alzheimer disease, RNA, Untranslated, RNA, Messenger