河北医科大学学报 ›› 2021, Vol. 42 ›› Issue (10): 1175-1179.doi: 10.3969/j.issn.1007-3205.2021.10.012

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新一代肿瘤标志物循环肿瘤细胞分型在宫颈癌诊断中的临床意义

  

  1. 上海交通大学医学院附属苏州九龙医院妇产科，江苏 苏州 215000
  • 出版日期:2021-10-25 发布日期:2021-10-28
  • 作者简介:张俊华（1986-），女，河南商丘人，上海交通大学医学院附属苏州九龙医院主治医师，医学硕士，从事妇科疾病诊治研究。

Clinical significance of circulating tumor cell typing as a new generation of tumor markers in the diagnosis of cervical cancer

  1. Department of Obstetrics and Gynecology, Suzhou Jiulong Hospital, Shanghai Jiaotong University, Jiangsu Province, Suzhou 215000, China
  • Online:2021-10-25 Published:2021-10-28

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摘要： 目的 探究宫颈癌患者外周血中循环肿瘤细胞（circulating tumor cell,CTC）各亚型和循环肿瘤微栓（circulating tumor microemboli,CTM）的分布情况，分析CTC/CTM与肿瘤标志物SCC-Ag、CA125、CA199表达水平的相关性。
方法 收集32例初治宫颈癌患者的肘静脉血，采用差相富集-肿瘤标志物免疫荧光染色-荧光原位杂交技术检测CTC/CTM，采用电化学发光免疫分析法检测血清SCC-Ag、CA125、CA199的表达水平。并分析CTC/CTM与病理特征关系和血清肿瘤标志物的相关性。
结果 32例初治宫颈癌患者中，CTC阳性29例（阳性率为90.6%），CTM阳性9例（阳性率为28.1%）。不同淋巴结转移情况宫颈癌患者CTC阳性率差异有统计学意义（P＜0.05）。不同临床分期和淋巴结转移情况宫颈癌患者CTM阳性率差异有统计学意义（P＜0.05）。血清CA125阳性28例（阳性率为87.5%），CA199阳性28例（阳性率为87.5%），SCC-Ag阳性24例（阳性率为75.0%）。CTC、CTM与血清CA125、CA199和SCC-Ag表达水平无相关性（P＞0.05）。
结论 CTC/CTM监测可作为宫颈癌病情进展的重要指标。


关键词: 宫颈肿瘤, 肿瘤细胞, 循环, 循环肿瘤微栓

Abstract: Objective To explore the distribution of circulating tumor cell(CTC) subtypes and circulating tumor microemboli(CTM) in peripheral blood of patients with cervical cancer(CC), and to analyze the correlation between CTC/CTM and the expression levels of tumor markers SCC-Ag, CA125 and CA199. 
Methods The elbow venous blood was collected from 32 patients with initially diagnosed CC,the subtraction enrichment-immunofluorescence staining-fluorescence in situ hybridization(SE-iFISH) was employed to detect CTC/CTM, and the electro-chemiluminescence immunoassay was used to measure the serum levels of SCC-Ag, CA125 and CA199. The correlation between CTC/CTM and pathological features and serum tumor markers was analyzed. 
Results Among 32 cases within itially diagnosed CC, 29 cases were positive for CTC(positive rate of 90.6%), and 9 cases were positive for CTM(positive rate of 28.1%). There was a significant difference in the positive rate of CTC in CC patients with different lymph node metastasis(P＜0.05). There was a significant difference in the positive rate of CTM among CC patients with different clinical stages and lymph node metastasis(P＜0.05). In addition, 28 cases were positive for CA125(positive rate of 87.5%), 28 cases were positive for CA199(positive rate of 87.5%), and 24 cases were positive for SCC-Ag(positive rate of 75.0%). There was no correlation of CTC and CTM with the serum levels of CA125, CA199 and SCC-Ag(P＞0.05). 
Conclusion CTC/CTM monitoring can be used as an important indicator of the progression of CC.


Key words: uterine cervical neoplasms, neoplastic cells, circulating, circulating tumor microemboli

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