血清miRNA-21和miRNA-192表达与糖尿病肾病的相关性研究

doi:10.3969/j.issn.1007-3205.2019.12.024

河北医科大学学报

血清miRNA-21和miRNA-192表达与糖尿病肾病的相关性研究

1.西安交通大学第一附属医院检验科，陕西西安710061；2.陕西省西安市第八医院检验科，
陕西西安 710061；3.陕西省咸阳市第一人民医院内分泌科，陕西咸阳 712000

出版日期:2019-12-25 发布日期:2019-12-26
作者简介:许薇（1987-），女，陕西西安人，西安交通大学第一附属医院检验师，医学学士，从事医学检验学研究。

Correlation study between serumal expression of miRNA-21 and miRNA-192, and diabetic nephropathy#br#

1.Department of Laboratory, the First Affiliated Hospital of Communication University of Xi′an, Shaanxi
Province， Xi′an 710061，China; 2.Department of Laboratory, the Eighth Hospital of Xi′an, Shaanxi
Province， Xi′an 710061， China； 3.Department of Endocrinology， the First People′s
Hospital of Xianyang, Shaanxi Province， Xianyang 712000， China

Online:2019-12-25 Published:2019-12-26

摘要/Abstract

摘要： ［摘要］
目的探讨血清miRNA-21和miRNA-192表达与糖尿病肾病(diabetic nephropathy,DN)的相关性。
方法选择2型糖尿病（type 2 diabetes mellitus，T2DM）患者（DM组）38例、DN患者（DN组）27例及同期体检健康者(对照组)30例，采用实时荧光定量PCR（real time-PCR，RT-PCR）技术检测血清miRNA-21和miRNA-192表达，采用PHILIPS-IU22彩色多普勒超声诊断仪检测肾主动脉（main renalartery,MRA）收缩期的峰值速度（peak systolic velocity,PSV）及阻力指数（resistance index,RI）等血流相关参数，比较分析以上指标的变化与DN的关系。
结果 DN组和DM组RI明显高于对照组，DN组RI明显高于DM组，DN组PSV明显低于DM组和对照组(P＜0.05)。DN组和DM组miRNA-21表达高于对照组，miRNA-192低于对照组，DN组miRNA-21表达高于DM组，miRNA-192低于DM组(P＜0.05)。DN组血清miRNA-21与miRNA-192表达呈负相关（r=-0.837，P＜0.05）；miRNA-21表达与RI水平呈正相关（r=0.812，P＜0.05），与PSV水平呈负相关（r=-0.793,P＜0.05）；miRNA-192与RI水平呈负相关（r=-0.807，P＜0.05），与PSV水平呈正相关（r=0.782，P＜0.05）。DM组血清miRNA-21与miRNA-192表达呈负相关（r=-0.816，P＜0.05）；miRNA-21与RI水平呈正相关（r=0.714，P＜0.05）；miRNA-192表达与RI水平呈负相关（r=-0.745，P＜0.05）。
结论血清miRNA-21和miRNA-192表达与DN的发生发展有相关性，检测miRNA-21和miRNA-192表达可为DN的早期发现及治疗提供基因依据。

关键词: 糖尿病肾病, miRNA-21, miRNA-192

Abstract: ［Abstract］Objective〖HTSS〗To investicate the correlation between serumal expression of miRNA-21 and miRNA-192,and diabetic nephropathy(DN).
〖WTHZ〗Methods〖HTSS〗These patients with type 2 diabetes mellitus(38 cases, DM group) and DN(27 cases,DN group),as well as 30 healthy check-up population were performed detecting their serumal expression of miRNA-21 and miRNA-192 by real time-PCR(RT-PCR). Blood flow-related parameters such as peak velocity(PSV) of renal aorta(MRA) and resistance index(RI) were measured by PHILIPS-IU22 color Doppler ultrasound system.The relationship between the above indexes and DN would be described via the comparative analysis.
〖WTHZ〗Results〖HTSS〗RI of DN group and DM group was significantly higher than that of control group, and RI of DN group was significantly higher than that of DM group, PSV of DN group was significantly lower than that of DM group and control group(P＜0.05). The expression of miRNA-21 increased whereas expression of miRNA-192 decreased in DN group and DM group compared with those in control group, and a same trend towards expression of miRNA-21 and miRNA-192 was also found in DN group compared with those in DM group(P＜0.05). In DN group, the expression of miRNA-21 and miRNA-192 was negatively correlated(r=-0.837, P＜0.05). The expression of miRNA-21 was positively correlated with the levels of RI(r=0.812, P＜0.05), and was negatively correlated with the level of PSV(r= -0.793, P＜0.05) . The expression of miRNA-192 was negatively correlated with the levels of RI(r=-0.807, P＜0.05), and was positively correlated with the level of PSV(r=0.782, P＜0.05). In DM group, the expression of miRNA-21 and miRNA-192 was negatively correlated(r=-0.816, P＜0.05); miRNA-21 was positively correlated with RI level(r=0.714, P＜0.05); miRNA-192 was negatively correlated with RI level(r=-0.745, P＜0.05).
〖WTHZ〗Conclusion〖HTSS〗The expression of miRNA-21 and miRNA-192 in serum is related to the occurrence and development of DN. Detecting the expression of miRNA-21 and miRNA-192 can provide the gene basis for early identification and treatment of DN.

Key words: diabetic nephropathies, miRNA-21, miRNA-192

许薇1，姚少侠2*，苟小林3. 血清miRNA-21和miRNA-192表达与糖尿病肾病的相关性研究[J]. 河北医科大学学报, doi: 10.3969/j.issn.1007-3205.2019.12.024.

XU Wei1, YAO Shao-xia2*,GOU Xiao-lin3. Correlation study between serumal expression of miRNA-21 and miRNA-192, and diabetic nephropathy#br#[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.1007-3205.2019.12.024.

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血清miRNA-21和miRNA-192表达与糖尿病肾病的相关性研究

Correlation study between serumal expression of miRNA-21 and miRNA-192, and diabetic nephropathy#br#

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