黄芪多糖对糖尿病大鼠视网膜病变的保护作用

doi:10.3969/j.issn.10073205.2017.07.013

河北医科大学学报

黄芪多糖对糖尿病大鼠视网膜病变的保护作用

1.河北省唐山市冀东眼科医院眼科，河北唐山 063000;2.华北理工大学附属医院眼科，河北唐山 063000

出版日期:2017-07-25 发布日期:2017-07-05
作者简介:刘俊辉（1978-），女，河北唐山人，河北省唐山市冀东眼科医院主治医师，医学学士，从事眼科疾病诊治研究。

The protective effects of Astragalus polysaccharide on diabetic rats with retinopathy

1.Department of Ophthalmology, Jidong Ophthalmology Hospital, Tangshan City， Hebei Province，
Tangshan 063000, China; 2.Department of Ophthalmology, North China University of
Science and Technology Affiliated Hospital，Tangshan 063000, China

Online:2017-07-25 Published:2017-07-05

摘要/Abstract

摘要： ［摘要］〓
〖HTH〗目的〖HTSS〗〖KG*2〗观察黄芪多糖（Astragalus polysaccharides，APS）对糖尿病大鼠视网膜病变的治疗作用并探讨相关机制。
〖HTH〗方法〖HTSS〗〖KG*2〗将清洁级健康雄性SD大鼠45只随机分为正常对照组、糖尿病模型组和APS组各15只。使用链脲佐菌素腹腔注射制作糖尿病大鼠模型。造模后1周，APS组大鼠给予400 mg/kg APS灌胃，1次/d，连续治疗8周。治疗结束后，测量各组大鼠的血糖、体质量，ELISA法检测大鼠视网膜中肿瘤坏死因子α（tumor necrosis factorα，TNFα）和细胞间黏附分子1（intercellular adhesion molecule 1 ICAM1）的含量，免疫印迹方法检测大鼠视网膜血管内皮生长因子（vascular endothelial growth factor，VEGF）和pAkt蛋白表达。
〖HTH〗结果〖HTSS〗〖KG*2〗模型组和APS组血糖高于对照组，体质量低于对照组（P＜005）。模型组和APS组大鼠TNFα、ICAM1、VEGF和pAkt表达明显高于对照组，APS组TNFα、ICAM1、VEGF和pAkt表达低于模型组，差异有统计学意义（P＜005）。
〖HTH〗结论〖HTSS〗〖KG*2〗APS可降低糖尿病大鼠视网膜中TNFα、ICAM1、VEGF和Akt的水平，通过抗炎、减少白细胞在糖尿病病变的视网膜黏附、影响AktVEGF信号通路起到对视网膜的保护作用。

关键词: 糖尿病视网膜病变, 黄芪多糖, 大鼠

Abstract: ［Abstract］ Objective〖HTSS〗〓To study the therapeutic effects of Astragalus polysaccharide(APS) on diabetic rats with retinopathy, and to explore its mechanism.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Fortyfive cleaning degree healthy male SD rats were randomly divided into 3 groups, normal control group, diabetic model group and APS group(n=15/group). The diabetic rats were made by streptozotocin intraperitoneal injection. One week after modeling, rats in APS group was administrated orally with APS(400 mg/kg) once daily for consecutive 8 weeks. After treatment, the blood glucose and body weight of different groups were measured. The expression of tumor necrosis factor alpha(TNFα) and intercellular adhesion molecule 1(ICAM1) in retina of different groups were detected by ELISA. The relative expression of vascular endothelial growth factor (VEGF) and pAkt in retina of different groups were tested by western blot.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The blood glucose of model group and APS group was higher than control group, body weight of model group and APS group was lower than control group(P<0.05). The expression of TNFα, ICAM1, VEGF and pAkt in model group and APS group was higher than that of control group. The expression of TNFα, ICAM1, VEGF and pAkt in APS group was lower than that of model group(P<005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓APS could decrease the expression of TNFα, ICAM1, VEGF and pAkt in retina of diabetic rats. Its protective effect on the retina may be through antiinflammatory effect, reducing Leukocyte adhere to diabetic retina and affecting Akt VEGF signaling pathways.

Key words: diabetic retinopathy, astragalan, rats

刘俊辉1,李春江1,李玉涛1,2*. 黄芪多糖对糖尿病大鼠视网膜病变的保护作用[J]. 河北医科大学学报, doi: 10.3969/j.issn.10073205.2017.07.013.

LIU Junhui1, LI Chunjiang1, LI Yutao1,2*. The protective effects of Astragalus polysaccharide on diabetic rats with retinopathy[J]. Journal of Hebei Medical University, doi: 10.3969/j.issn.10073205.2017.07.013.

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黄芪多糖对糖尿病大鼠视网膜病变的保护作用

The protective effects of Astragalus polysaccharide on diabetic rats with retinopathy

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