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    25 February 2017, Volume 38 Issue 2
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    The preliminary study on correlation between APOC1 and vascular neointimal formation#br#
    CHEN Hanbing， ZHANG Yuqing， LIU Jinyao， LIU Lingdi， NIE Lei， HAN Mei*
    2017, 38(2):  125-128.  doi:10.3969/j.issn.10073205.2017.02.001
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    ［Abstract］ Objective〖HTSS〗〓To discuss the correlation of apolipoprotein (APO) C1 expression and neointimal formation.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The intimal hyperplasia model of the mice was prepared by the common carotid artery ligation. HE staining was used to test the neointima. Western blot was used to detect the protein expression of APOC1 and SM22α, Immunofluorescence was used to detect the protein expression and distribution of APOC1.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓With the extension of time of ligation,the intimal hyperplasia was observed in the carotid arteries of mice after the ligation. At different time points of the common carotid artery ligation. the expression of SM22α was decreased in neointimal region. The expression of APOC1 was gradually increased, which was contrary to that of SM22. Immunofluorescence staining also showed that the fluorescence intensity of APOC1 was enhanced in the area of thickened intima, and it is positively correlated with the thickness of intima.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The expression of APOC1 may be involved in vascular smooth muscle cell switching and vascular remodeling.
    The apoptosis regulation on mice breast cancer cell line 4T1 by TTP #br#
    ZHU Guangkui1, HE Wenhui1,ZHENG Ying2， LIU Jiaju1, CHENG Zili1, QIAN Xuesong3*
    2017, 38(2):  129-132.  doi:10.3969/j.issn.10073205.2017.02.002
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    ［Abstract］ Objective〖HTSS〗〓To establish a mouse breast cancer cell line 4T1 with high TTP (trisetetraprolin) expression and to observe the effect of TTP on breast cancer cell line 4T1 apoptosis.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The breast cancer 4T1 cell line was stably transfected with the plasmids of PCR3.1 and PCR3.1/mTTP by Lipofectamine 2000. The cell apoptosis was detected using flow cytometry technology.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Higher mTTP expression was detected in 4T1 cells that has been transfected with PCR3.1+mTTP plasmid using real time quantitative PCR. The apoptosis rate among 4T1, 4T1 PCR3.1 and 4T1 PCR3.1+mTTP was not significant difference. But after treatment the cells with Staurosporine, a apoptosis promoting medicine, for 6 hours, apoptosis rate of 4T1 PCR3.1+mTTP cell line was increased. The apoptosis rate of 4T1, 4T1 PCR3.1 and 4T1 PCR3.1+mTTP was 17%, 18% and 29.5%, respectively.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓mTTP could not enhance 4T1 cell apoptosis directly, but it could increase the sensitivity of 4T1 to apoptosis inducing medicine.
    The therapeutic effects of Rhodosin on cognition in AD rats
    LIU Na1, WANG Jinhua1, WANG Hualong1, QIU Huiqing1, WANG Yanyong1,2*， WANG Mingwei1,2
    2017, 38(2):  133-137.  doi:10.3969/j.issn.10073205.2017.02.003
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    ［Abstract］ Objective〖HTSS〗〓To study the therapeutic effects of Rhodosin on cognitive function in Alzheimer disease(AD) rats.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The AD rat models and Rhodosin intervention models were established. Learning and memory abilities of rats were tested by the Morris water maze. The protein expressions of AKT, pAKT, GSK3β, PGSK3β, bcl2 and bax were examined via Western blotting. And the ultrastructure of hippocampus neurons was determined by transmission electron microscopy.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓After modeling, the latent period of the model group and Rhodosin group was prolonged than that in the control group. After treatment, the latent period of the model group and Rhodosin group was prolonged than that in the control group. The Rhodosin group was shorter than the model group(P＜005). After modeling, the stay time of the target quadrant in model group and Rhodosin group was significantly shorter than the control group, the difference was statistically significant(P＜005).  After treatment, the stay time of the target quadrant in model group and Rhodosin group was also shorter than the control group, but the Rhodosin group was longer than the model group(P＜005). The protein expressions of pGSK3β in the model group and Rhodosin group was lower than that in the control group, Rhodosin group higher than model group. The protein expressions of pAKT in Rhodosin group was higher than control group and model group, model group lower than control group(P＜005). The protein expressions of bcl2 in model group lower than control group, Rhodosin group was higher than control group and model group(P＜005). The bax protein expressions in model group was high than control group and Rhodosin group(P＜005). 
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓 Rhodosin could improve cognitive function in AD rats. Its mechanisms may be through upregulating antiapoptotic protein bcl2 and downregulating proapoptotic protein bax via the PI3K/AKT/GSK3β pathway.
    Prevalence of thyroid nodules in mainland China:a Metaanalysis
    HUO Lishuang1, LIU Fengyu2, GAO Chen1, YANG Xiaoyan3, YUAN Shengfang4, ZHAO Zhansheng1*
    2017, 38(2):  138-141,205.  doi:10.3969/j.issn.10073205.2017.02.004
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    ［Abstract］  Objective〖HTSS〗〓To understand the prevalence of thyroid nodules in mainland China by using Metaanalysis.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Literature search was conducted to collect studies on the prevalence of thyroid nodules patients in China National Knowledge Internet, Chinese science＆technology journal database and Wanfang database. Stata 12.0 software was used to estimate the prevalence of thyroid nodules，as well as on gender，age, degree of education and other factors to make subgroup analysis.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓A total of 68 studies ,including a total size of 560 877 and 190 228 patients of thyroid nodules, were eligible for inclusion in the review.The pooled analysis showed that prevalence of thyroid nodules was 32%［95%CI 30%-34%］，the prevalence rates of thyroid nodules appeared 26% in male and 39% in female.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Incidence rate of thyroid nodules was high and increased with the age increased.More cases were found in females than in males. The higher of cultural degree，the higher the prevalence of thyroid nodules. The prevalence of thyroid nodule in Hebei, Liaoning and Beijing area is higher in different provinces.  Low iodine intake may increase the risk of thyroid nodule. An increased risk of thyroid nodule was respectively associated with obesity and abnormal glucose metabolism.
    Study of the clinical effects of calcitriol on the urinary albumin excretion rate of patients with diabetic nephropathy
    HE Lifang1, LI Qingke2, ZHANG Xiuxiang1, ZHOU Xiangjuan1, WANG Yanzhen1, MA Yuxia1
    2017, 38(2):  142-145.  doi:10.3969/j.issn.10073205.2017.02.005
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    ［Abstract］ Objective〖HTSS〗〓To observe the security and the clinical effects of calcitriol combined with valsartan on patients with diabetic nephropathy（DN）.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Sixty patients with DN were randomly divided into two groups (control group and observation group). The control group were given valsartan and the observation group were given calcitriol and valsartan. Before and after treatment for six months. 24hour urinary protein quantification（UTP), urinary albumin excretion rates(UAER), highsensitive Creactive protein（hsCRP）, kalium(K), calcium(Ca), phosphorus(P), serum creatinine(SCr）, parathyroid hormone(PTH) were detected.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓UTP, UAER, hsCRP of the patients in the two group after treatment was significantly decreased, the difference was statistically significant（P＜005）. After treatment, the UTP, UAER and hsCRP in the observation group were lower than those in the control group（P＜005）. PTH in the 2 groups before and after treatment did not change significantly, the difference between the 2 groups before treatment and after treatment were not statistically significant（P＞005）.  K， Ca， P， SCr in the 2 groups were no obvious change before and after treatment. The difference was not statistically significant (P＞005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Calcitriol combined with valsartan can effectively reduce the urinary albumin excretion rate of patients with diabetic nephropathy,and the curative effect is better than alone.
    Analysis of related factors of metabolic syndrome associated nephropathy
    HAO Yujie， DONG Yanfei， YIN Xiaowen， WANG Yunhong， ZHANG Peixing
    2017, 38(2):  146-150.  doi:10.3969/j.issn.10073205.2017.02.006
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    ［Abstract］  Objective〖HTSS〗〓To explore the related factors of metabolic syndrome associated nephropathy.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Eightysix obese patients （1 lost） for routine renal function detection in our hospital from March 2010 to March 2014 were selected, who were divided into two groups, 31 patients with normal renal function as group A, and 54 patients with renal injury as group B according to the testing results. All selected patients were treated, and the group B was divided into group B1(renal function were significantly improved,31 cases) and group B2(renal function did not improved,23 cases) according to the result of 12 weeks treatment. The lipids, blood rheology,renal function, blood pressure and glucose of the two groups were detected first came to medical examination and after 12 weeks treatment.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The total cholesterol(TC), triglyceride(TG), fasting blood glucose(FBG), 2hour postprandial glucose(2 hPBG), fasting insulin(FINS), and homeostasis model assessmentinsulin resistance index(HOMAIR) of group B were higher than that of the group A, high density lipoprotein cholesterol(HDLC) was significantly lower that that in group A, the difference was statistically significant(P＜005). The difference of low density lipoprotein cholesterol(LDLC) levels in 2 groups were not statistically significant(P＞005). 24hour urinary protein excretion(24 hUP), blood urea nitrogen(BUN) and mean arterial pressure(MAP) level were significantly higher than that in group A, eGFR level was significantly lower than that in group A(P＜005). The TC, TG, 24 hUP, BUN, FBG, HOMAIR and MAP of group B1 were lower than that in group A, the eGFR of group B1 was significantly higher than that in group B2, the difference was statistically significant(P＜005). Before treatment the blood rheology index of both A, B groups had no significant difference(P＞005); after the treatment the blood rheology index of both A, B groups were significantly lower than that before treatment, blood rheology index of B group were significantly lower than that in group A, the difference was statistically significant(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓HOMAIR and MAP were important cause of the rise of metabolic syndrome associated nephropathy, and can be pointed out for the prevention and treatment of disease.
    Overexpression of DNAJC6 induces the occurrence of EMT in hepatocellular carcinoma cells
    GENG Yunfeng1， LI Xiaona2， LU Yujuan1， JIA Jinguang1,YANG Tao1*
    2017, 38(2):  151-153,158.  doi:10.3969/j.issn.10073205.2017.02.007
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    ［Abstract］ Objective〖HTSS〗〓To investigate the role of DNAJC6 in the progression of hepatocellular carcinoma in vitro.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The effects of DNAJC6 expression on epithelialtomesenchymal transition(EMT) in HepG2 cells were detected by in vitro liver cancer cell culture, plasmid construction, transfection and reverse transcriptionpolymerize chain reaction.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Compared with the control group，the expression level of EMT related transcription factor in hepatocellular carcinoma cell line HepG2 transfected with DNAJC6 Twist1, Snail, Slug, ZEB1 was increased significantly(P＜001). Mesenchymal cell markers Ncadherin and vimentin were upregulated(P＜001). Epithelial markers Ecadherin and ZO1 were downregulated(P＜001).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓DNAJC6 is involved in the process of hepatocellular carcinoma by inducing EMT.
    The characteristics of different methods to create scoliosis model of bipedal rats
    LI Pengfei, JIA Nan, ZHAO Peng, YANG Jianbo, LIU Jinlu, JIN Xianhui*
    2017, 38(2):  154-158.  doi:10.3969/j.issn.10073205.2017.02.008
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    ［Abstract］  Objective〖HTSS〗〓To explore the mechanism and the significance of different methods to establish scoliosis model of bipedal rats.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Forty SD rats were randomly divided into four groups. The front limb and tail of SD rats was completely removed. The control group only create model of bipedal rats. Ten bipedal rats in group A were given intraperitoneal injection of 6mercaptopurine(30 mL/kg). Tenbipedal rats in group B received intraperitoneal injection of luzindole(0.2 mg/kg). 10 bipedal rats in group C received intramuscular injection of botulinum toxin A. The spinal curvature index, change of intervertebral height and the characteristics of scoliosis were compared between the four groups before and after operation.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓All the experimental animals survived to complete the modeling experiment of scoliosis. In control group, there was no scoliosis. All three experimental groups had scoliosis. Cobb's angle of rats were all increased in four groups and showed an raising trend. However, cobb's angle of the 6 mercaptopurine group increased the maximum. Vertebral height of rats in four groups were all increased and showed a rising trend. Compared with control group, the height of vertebral body was lost in 6mercaptopurine group and botulinum toxin group. However, the vertebral height of low melatonin group height was increased. There were significant differences among groups, time points, interaction of group and pointintime(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Application of intraperitoneal injection 6mercaptopurine can establish a severe scoliosis model. Intraperitoneal injection of luzindole proved that low concentration of melatonin can induce scoliosis. The botulin can damage the fiber tissue of paraspinal muscle. It is proved that destruction of biomechanical balance of paraspinal muscle can creat scoliosis models.
    The influence of residual oligohydramnios on perinatal outcome of early preterm premature rupture of membranes
    TONG Chongxin1, LIU Jing2, SHAN Ling1, YIN Hongya1
    2017, 38(2):  159-163.  doi:10.3969/j.issn.10073205.2017.02.09
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    ［Abstract］ Objective〖HTSS〗〓To explore the complication, delivery model of pregnancy and the outcomes of premature infant on early preterm premature rupture with residue oligohydramnios.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Two hundred and twentyfive cases of 26-33+6 weeks of singletons pregnant women were divided into four groups. Sixtyseven cases of residue oligohydramnios were group A, including group A1 which is 32 cases of 26-31+6 weeks of singletons pregnant women and group A2 which is 35 cases of 32-33+6 weeks of singletons pregnant women . One hundred and fiftyeight cases of normal amniotic fluid were group B, including group B1 which is 78 cases of 26-31+6 weeks of singletons pregnant women and Group B2 which is 80 cases of 32-33+6 weeks of singletons pregnant women .The indexes such as the complication, delivery model of pregnant and the outcomes of premature infant were compared in the four groups.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓In group A1,WBC count is above than that in group B1 and B2; Creactive protein is higher than that in group A2, B1, B2. Latency period is lower in group A1 than that in group B1 and B2, but is higher than that in group A2, also it is lower in group A2 than group B1 and B2, it is higher in group B1 than that in group B2. The cesarean section rate of group A1 is higher than group A2, B1, B2. Chorioamnionitis is higher in group A1 than in group B1 and B2, it is also higher in group A2 than that in group B2. Fetal distress is above in group A1 than in group A2, B1, B2. Amniotic fluid contamination is lower in group B1 and B2 than that in group A1 and A2. Puerperal infection is higher in group A1 than that in group B1 and B2. There are significant difference in these groups(P＜005). The premature infants asphyxia rate is lower and the death rate is higher in group A1 than that in group B1 and B2. Apgar score 1 min≤7 is higher in group A1 than that in group B1 and B2, also it is higher in  group A2 than group B2. Apgar score 5 min≤7, assisted respiration and intrauterine infection are all higher in group A1 than that in group B1 and B2. Umbilical cord inflammation in group A1 and A2 is above than that in group B2. The pH of umbilical arterial is lower in group A1 and A2 than that in group B1 and B2. There are significant difference in all these groups(P＜005). In group A1 and A2 with AFI≤20 mm, neonatal mortality and cesarean section rate are significantly different in the two groups(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Patients of residue oligohydramnios with early preterm premature rupture of membranes should be monitored closely，the smaller of the gestational age, the greater damage of the mother and children. In the same gestational age, residual oligohydramnios were associations with the higher rate of the cesarean section, maternal infection, premature infants asphyxia and mortality; also were associations with the shorter of latency period. The mortality of premature infants of 26-31+6 weeks with AFI≤20 mm is more higher, the cesarean delivery should be choosed carefully.
    Application value of HPV16DNA integration in screening and prediction of cervical lesions
    WANG Lili, ZHANG Lin, WANG Xiaona
    2017, 38(2):  164-167.  doi:10.3969/j.issn.10073205.2017.02.010
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    ［Abstract］ Objective〖HTSS〗〓To evaluate the correlation between physical status of human papillomavirus 16（HPV16） and degree of cervical lesion and the role of HPV16 integration in prediction and diagnosis of high squmous intrepithelil lesion(HSIL).
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 165 cervical cells samples were collected from the patients with HPV positive. All patients′ pathological results were definite, including 18 cases of cervicitis, 24 cases with cervical intraepithelial neoplasia（CIN）1, 45 cases with CIN2, 51 cases with CIN3 and 27 cases with cervical cancer. The total genomic DNA were extracted and multiplex PCR were performed for detection of the level of HPV E2 and E6 genes. Comparing the physical status of HPV16 in different degrees of cervical lesions according to the E2/E6 ratio.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The single infection ratios of HPV16 in cervicitis, CIN1, CIN2, CIN3 and cervical cancer were 6.73%(7/104), 10.58%(11/104), 29.81%(31/104), 30.77%(32/104), 22.11%(23/104), respectively. Single HPV infection rate was gradually increasing with the processing of cervical lesion level. The integration ratios of HPV16 in cervicitis, CIN1, CIN2, CIN3 and cervical cancer were 16.67%(3/18), 20.83%(5/24), 46.67%(21/45), 62.74%(32/51), 77.78%(21/27), respectively. The difference was statistically significant(P<005). The sensitivity and specificity of diagnosis of cervical lesions by HPV16 DNA integrate status were 60.16% and 79.07% and the positive predictive value and negative predictive value were 40.96% and 90.24%.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The E2/E6 ratio of HPV16  can be used as indicators to judge the state of viral integration, and the integration status of HPV16 was accompanied with the processing of cervical lesion level.
    Expression of OPN in tissue of the paitents with endometrial carcinoma and effect on the prognosis
    LI Meiyan
    2017, 38(2):  168-172.  doi:10.3969/j.issn.10073205.2017.02.011
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    ［Abstract］ 〖JP2〗Objective〖HTSS〗〓To detect the expression of osteopontin(OPN) in tissues of endometrial〖JP〗 carcinomas and to analyze its relationship with clinicopathologic features(age, clinical and surgical stage, histodifferentiation, myometrial invasion, histological type, lymph node metastasis) in order to investigate the effect on the prognosis of endometrial carcinoma.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One hundred and seventyone patients by pathological examination to be endometrial carcinoma were selected. The expression of OPN was identified by immunohistochemically SP method in 171 paraffin block. The relationship on the OPN expression and the prognosis of endometrial carcinoma was discussed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The positive expression rate of OPN was 63.74%(109/171) in 171 patients with endometrial carcinoma. The differences of OPN positive status with different situation of clinical and surgical stage, histodifferentiation, myometrial invasion and lymph node metastasis were statistical significance(P＜005). KaplanMeier method showed that OPN, clinical and surgical stage, myometrial invasion and lymph node metastasis were related with survival rate of endometrial carcinoma(P＜005). Cox analysis showed that OPN, clinical and surgical stage, myometrial invasion were risk factors of endometrial carcinoma prognosis.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓OPN was an important factor effect on prognostic of endometrial carcinoma, and it had a great clinical significance in judging prognostic and guiding clinical treatment of endometrial carcinoma.
    Necessity analysis of routine screening for cervical during the 42 days of postpartum and gestational period
    JIN Yanqi， HUANG Yongtong， ZHU Xiaoli
    2017, 38(2):  173-176.  doi:10.3969/j.issn.10073205.2017.02.012
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    ［Abstract］  Objective〖HTSS〗〓To discuss the necessity analysis of routine screening for cervical during the 42 days of postpartum and gestational period.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Six hundred and twentysix cases of women were selected, including pregnancy 6-36 weeks in 60 cases, postpartum 42 d in 566 cases. The distribution of cytological diagnosis and cervical changes seen by the naked eye had been observed after pregnancy and at 42 d postpartum, by using the gold standard of the Bethesda system diagnosis.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The positive rate of cytological screening in 60 pregnant women was 11.68%(7/60) in this research. The positive rate was 7.42%(42/566) of 566 cases of pregnant women in this group of studies by cytological screening on the 42nd day postpartum. There were 417 cases were delivered by vaginal delivery and 149 cases of cesarean section of 566 cases of pregnant women in this group of studies. The total constituent ratio was 73.85%(418/566) of cervical lesions, including cervicitis in 388 cases, cervical laceration in 9 cases, cervical hypertrophy in 5 cases, cervical polyps in 4 cases, developmental malformation in 4 cases, cervical incompetence in 3 cases, cervical gland cyst in 2 cases, cervical intraepithelial neoplasia grade 2 in 1 cases, cervical intraepithelial neoplasia grade 1in 2 cases.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Pregnant women should be screened for cervical disease during the period of prenatal and postnatal 42 d examination, which is helpful for the timely detection of cervical lesions, and to detect and treatment early in order to improve the quality life of pregnant women.
    Analysis of risk factors of infantile cholestatic hepatopathy
    CHEN Yuan, MA Xueling, GENG Jianghui, ZHANG Shaodan
    2017, 38(2):  177-179,183.  doi:10.3969/j.issn.10073205.2017.02.013
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    ［Abstract］  Objective〖HTSS〗〓To explore the high risk factors  in infantile cholestatic hepatopathy(ICH).
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The clinical data of 86 infants with cholestatic hepatopathy were reviewed and analyzed. Meanwhile, 100 cases who were hospitalized of the same term were as the control.  Children's gender, age, feeding history, history of jaundice in the neonatal period, stool color, skin yellow dye history, serum alanine, transaminase(ALT), serum total bile acids(TBA) were summarize. Logistic regression analysis was been applied to obtain the result.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The result of logistic regression analysis showed that breast feeding, pathological jaundice in neonatal period, the history of jaundice in skin or sclera and higher level of ALT are the high risk factors for ICH（P＜005）.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓As the disease causes of ICH, breast feeding, pathological jaundice in neonatal period, the history of jaundice in skin or sclera and higher level of ALT are the high risk factors. While artificial feeding are protective factors.
    Study on the relationship between sleep apnea syndrome and simple obesity in children#br#
    YU Ling1， WANG Lei2， DI Baochun3， YU Changli4*
    2017, 38(2):  180-183.  doi:10.3969/j.issn.10073205.2017.02.014
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    ［Abstract］ Objective〖HTSS〗〓To investigate the sleep symptoms and polysomnographic patterns of obstructive sleep apnea in obese children and to explore the relationship between the index of weight for height and obstructive sleepapnea/hypopnea syndrome(OSAHS) parameters.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Sixtyeight simple obese children(age 2-12 years old) with OSAHS and the same period to hospital physical examination of seventy healthy children were enrolled in this study. Polysomnography(PSG) was done for the diagnosis of obstructive sleep apnea and every child was monitored with PSG. According to the weight for height index of the OSAHS, groups were divided into mild, moderate and severe obesity group. The mean oxygen saturation(MSaO2)， the lowest oxygen saturation (LSaO2)， more than 3% of the oxygen desaturation index(ODI), SaO2 less than or equal to 90% and the weight for height index were recorded. PSG data of OSAHS group and control group were recorded and analyzed.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓The apneahypopneaindex(AHI) and ODI of patients in group OSAHS were significantly higher than those in control group. LSaO2 was significantly lower than the control group, the difference was statistically significant(P＜005). AHI of severe OSAHS patients was higher than that of mild and moderate(P＜005), and there was no statistically significant difference between mildtomoderate group(P＞005). The ODI of moderate and severe subgroups were higher than those in mild group(P＜005), the ODI of severe subgroups were higher than those in moderate subgroups(P＜005). In mild subgroups, moderate and severe subgroup，MSaO2 was higher and LSaO2 was lower than those in mild group(P＜0.05).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Obese children have a higher risk for OSAHS，and the severity of obesity was positively related to the severity of OSAHS.
    The common factors of affecting bedside mobile DR image quality and related dynamic control and management
    GUO Zhe， WU Wenjuan*， ZHONG Zhiwei， ZHANG Zuzhuo， MENG Hui， LI Xiaona
    2017, 38(2):  184-187.  doi:10.3969/j.issn.10073205.2017.02.015
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    ［Abstract］  Objective〖HTSS〗〓To improve the quality of the beside mobile digital radiography（DR） image and reduce the patients' radiation dose by analyzing the common factors of affecting bedside DR image quality to achieve dynamic control and management.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One hundred and nine unqualified beside DR images of different body parts were collected. The images were recorded. Regarding the phenomena and reasons of the unqualified DR images, 3 experienced and professional radiographers analyzed, discussed and counted all the images one bye one with reference to the topclass hospitals' digital image evaluation standard from the Ministry of Health.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Among the 109 images, 31 cases(28.4%) of adjustment of postprocessing parameters and 29 cases(26.6%) of selection of radiographic conditions are inappropriate. Eight cases(7.3%) of radiography positions are not standard. Two cases(1.9%) of recording the patients' information and 3 cases(2.7%) of marking the left and right numbers are wrong. Two cases(1.9%) of the typesetting and printing format are not standardized . Eight cases(7.3%) of the proportion of the photo image are uncoordinated. 6 cases(5.5%) of radiation field were too large or too small. There are 9 cases(8.3%)  of artifact for the extracorporeal foreign matter. Two cases(1.9%) of the incidence of the Xray center line are incorrect. There are grid decentering effect in 9 cases(8.2%).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Selecting the suitable photography conditions, operating strictly according to the standard rules, using the postprocessing techniques appropriately, adjusting and matching the postprocessing parameter are all the key points to improve the quality of the bedside DR image.
    The clinical value of MRI pulmonary artery angiography in the diagnosis of pulmonary artery embolism#br#
    LI Wen1， JI Junyu1， BAI Hongzhong2*， GENG Guang1， HAO Meng1， JIA Jianlei1
    2017, 38(2):  188-191,200.  doi:10.3969/j.issn.10073205.2017.02.016
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    ［Abstract］ Objective〖HTSS〗〓To discuss the value of MR T2trufi bright blood sequence and contrastenhanced magnetic resonance pulmonary angiography（MRPA） in the diagnosis of pulmonary artery embolism.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Thirty healthy adult Chinese white rabbit were chosen as experimental animal and were examined using computer tomography pulmonary angiography(CTPA)、 MRPA and MR T2trufi bright blood sequence separately. Their pulmonary arteries were graded by vessel diameter. The basic values such as vessel diameter, vessel edge and density in each grade of pulmonary artery were compared with each other and with the results of pulmonary artery embolism.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓①In the display of vessel diameters, MRPA and CTPA, MR T2trufi bright blood sequence had no significant difference in the display of gradeⅠpulmonary artery(P＞005); MR T2trufi bright blood sequence detected a larger diameter than MRPA and CTPA in gradeⅡpulmonary artery(P＜005); MRPA had a larger diameter than CTPA and MR T2trufi bright blood sequence in Ⅲ and Ⅳpulmonary artery(P＜005). ②In the display of vessel clarity, CTPA was superior than MR T2trufi bright blood sequence and MRPA in gradeⅠpulmonary artery(P＜005); CTPA was superior than MR T2trufi bright blood sequence and MRPA in gradeⅡpulmonary artery, and MRPA was the worst(P＜005); In grade Ⅲ and Ⅳpulmonary artery, CTPA was superior than MR T2trufi bright blood sequence(P＜005), and MRPA had no significant difference with MR T2trufi bright blood sequence(P＞005). ③In the detection of pulmonary emboli, CTPA and MR T2trufi bright blood sequence had significant difference in gradeⅡpulmonary artery(P＜005); CTPA and MR T2trufi bright blood sequence , MRPA had significant difference in grade Ⅲ pulmonary artery(P＜005).
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓MR T2trufi bright blood sequence and MRPA pulmonary artery angiography had obvious advantage in the diagnosis of pulmonary artery embolism, but the examination time and signalnoise ratio are inevitable pitfalls. MR can not replace CTPA examination up till now, but can be an beneficial supplement.
    Correlation analysis between ATP, 2,3DPG concentration in suspended red blood cells without leukocyte and Oxidative stress during storage
    WANG Suling1, HAN Wei1, HE Lujun1, WANG Yanbin1, WANG Qie2*
    2017, 38(2):  192-195.  doi:10.3969/j.issn.10073205.2017.02.017
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    ［Abstract］ Objective〖HTSS〗〓To investigate the correlation between the change of  adenosine triphosphate（ATP）, 2,3diphosphoglycerate（2,3DPG) concentration and oxidative stress level in suspended red blood cells without leukocyte during storage.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The blood of volunteers were collected to prepare suspended red blood cells without leukocyte. The suspended red blood cells were stored at 2-6 ℃. On day 7, day 14, day 28 and day 42 of storage, the concentration of reactive oxygen species（ROS) in red blood cells was detected by fluorescence microplate reader, the concentration of hydrogen peroxide（H2O2) outside cell was detected with spectrophotometric method, the concentration of ATP was detected with chemical chromatometry, the 2,3DPG was detected with Uv test method. Correlation analysis between the ROS, H2O2, ATP, 2,3DPG and storage time were carried out, respectively. Correlation analysis between ATP, 2,3DPG and ROS, H2O2 were carried out, respectively.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓With the extension of storage time, the concentration of ROS and H2O2 were significantly increased， the ROS and H2O2 content on day 42 of storage were significantly higher than that of day 7, day 14 and day 28. There were positive correlation between ROS and storage time, between H2O2 and storage time. But with the extension of storage time, the concentration of ATP and 2,3DPG were significantly decreased. The ATP and 2,3DPG content on day 42 of storage were significantly lower than that of day 7, day 14 and day 28. There were negative  correlation between ATP and storage time, between 2,3DPG and storage time. There were negative correlation between ATP and ROS, between ATP and H2O2, between 2,3DPG and ROS, between 2,3DPG and H2O2.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓With the extension of storage time, the concentration of ATP and 2,3DPG in suspended red blood cells without leukocyte were gradually reduced, which is closely related with the increasing of oxidative stress reaction. 
    Analysis on external quality assessment for national prepregnancy health examination project
    GAO Zhangquan1,2, LI Guozheng1,2*, ZHAO Jianhong3, CHEN Zhuaisheng1,2, WANG Shuhong1,2, LIU Xiaolei3
    2017, 38(2):  196-200.  doi:10.3969/j.issn.10073205.2017.02.018
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    ［Abstract］ Objective〖HTSS〗〓To analyze the results of the national and the provincial external quality assessment(EQA) of the county agencies clinical Laboratory that taking part in the prepregnancy health examination project in Hebei province. New suggestions and countermeasures are put forward for the next step.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The results of every county agencies was reviewed using the method of retrospective analysis.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓From the results of national EQA, compared to 2012 and 2013, the excellent rate in 2014 is higher and higher, while the failure rate is lower and lower(P<005). Hebei provincial EQA showed that the total feedback rate in 2015 has reached 100.00%. The excellent rate in 2015 is 59.07% percent(101/171), significantly increased than 44.55% (49/110) in 2013(P<005). The proportion of HBcAb, syphilis antibody, senum creatinine, WBC, RBC(＞80-100) from 171 institutions of Hebei province is increased significantly in 2015 than in 2013, the difference was statistically significant(P<005) in 2015 .
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Since the launch of the national prepregnancy health examination project，the quality of clinical examination in Hebei province is increasing year by year，but there are still some room for improvement.
    Simultaneous determination of rhein,emodin, chryophanol,physcion and stibene glucoside in compound preparations by HPLC
    ZHANG Xia, CHENG Xiaoye, LIAO Man, ZHANG Yuqian, ZHANG Lantong*, FAN Shuyan
    2017, 38(2):  201-205.  doi:10.3969/j.issn.10073205.2017.02.019
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    ［Abstract］ Objective〖HTSS〗〓To develop an analysis method for Rhein, Emodin, Chrysophanol and Physcion in Mingmu Shangqing tablets and stibene glucoside in Jiangzhining tablets by high efficiency liquid chromatography（HPLC）.
    〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The four constituents of Mingmu Shangqing tablets were separated on Kromasil C18 column (250 mm×4.6 mm, 5 μm) using a mobile phase consisted of methanoltetrahydrofuran1% phosphoric acid in water at the detection wavelength of 254 nm. The constituent stibene glucoside in Jiangzhining tablets was separated on Kromasil C18 column (250 mm×4.6 mm, 5 μm) using a mobile phase consisted of methanol 0.1% phosphoric acid in water at the detection wavelength of 320 nm.
    〖HTH〗〖WTHZ〗Results〖HTSS〗〓Rhein, Emodin, Chrysophanol and Physcionhad had a good liner relationship within the scope of 0.500 to 4.00(r=0.999 3), 0.370 to 2.96(r=0.999 0), 1.10 to 8.80(r=0.999 0), 1.00 to 8.00(r=0.999 0) ng, respectively. And their regression equation were Y=8.60×103+9.62×104X, Y=-1.16×104+1.20×105X, Y=7.41×103+1.20×105X, Y=4.13×103+6.91×104X, respectively. The average recoveries（n=6）were 97.8%， 97.8%， 98.7%， 99.5% and with relative standard deviation（RSD）（n=6） of 1.2%， 0.89%， 1.7%， 1.7%. Stibene glucoside had a good liner relationship within the scope of 0.218 to 1.31 ng(r=0.999 0). The regression equation was Y=-6.33+3.18×103X. The average recoveries were 96.6% and with RSD of 0.65%.
    〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The method is rapid,simple,accurate and with good reproducibility and can be used in quality control of Mingmu Shangqing tablets and Jiangzhining tablets.