Table of Content

25 January 2017, Volume 38 Issue 1

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Relationship between serum adipocyte fatty acidbinding protein and islet beta cell function in individuals with different glucose tolerance

BAI Jing, LEI Ying， QI Fanfan， WANG Xue， WANG Zhanjian， ZHOU Yaru*

2017, 37(1):  1-5,10.  doi:10.3969/j.issn.10073205.2017.01.001

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Abstract］ Objective〖HTSS〗〓To investigate the level of serum adipocyte fatty acidbinding protein(AFABP) and the relationship between serum AFABP and body mass index(BMI), blood pressure, blood glucose, blood lipid, HOMAinsulin resistance index(HOMAIR), insulin levels in Han Chinese population with different glucose tolerance.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 120 subjects were enrolled in this study. They were divided into 3 groups according to 75 g oral glucosetolerancetest(OGTT), including normal glucose tolerance(NGT, n=26), impaired glucose regulation(IGR, n=32), and type 2 diabetes mellitus(T2DM, n=62). The T2DM group was divided into 2 subgroups by BMI according to the guidelines of the prevention and treatment adiposity in China, which included group A(nonobese subjects) and group B(obese subjects). Clinical and biochemical metabolic parameters were measured.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The levels of serum AFABP were significantly higher in T2DM subjects than those of IGR and NGT subjects(P＜001). Compared with NGT group, fasting serum AFABP levels were significantly increased in IGR group(P＜001). Compared with T2DM group, BMI、WC、HC、UA、FINS、HOMAIR、HOMAβlevels were significantly increased in IGR group(P＜005). The level of serum AFABP was significantly higher in T2DM obese subjects than T2DM nonobese subjects(P＜001). Serum AFABP was correlated positively with BMI、WC、HC、UA、TC、TG、FPG、2 hPG、AUCGLU and HOMAIR(all P＜005). There was no correlation between serum AFABP and LDLC、HDLC、SBP、DBP、FINS、AUCINS、HOMAβ(all P＞005). Multiple regression analysis showed that BMI, HbA1c and UA were independently related factors for serum AFABP levels.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The level of AFABP is associated with obesity, insulin resistance, abnormal glucose and lipid metabolism. Measurement of serum AFABP concentrations might be useful for diagnosis and prevention of T2DM.


Microdisorders of thyroid axis in patients with type 2 diabetes and obesity, and their impact on insulin resistance

GONG Min， WANG Aihua， LI Xiucai

2017, 37(1):  6-10.  doi:10.3969/j.issn.10073205.2017.01.002

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［Abstract］  Objective〖HTSS〗〓To observe the microdisorders of thyroid axis in patients with type 2 diabetes mellitus(T2DM) and obesity, and their impact on insulin resistance.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Two hundred patients with T2DM and first discovery of obesity were selected as T2DM group. One hundred obesity persons were selected as normal glucose tolerance(NGT) group at the same period. The levels of free triiodothyronine(FT3), free thyroxine(FT4), thyroid stimulating hormone(TSH), homeostasis model of assessment for insulin resistance(HOMAIR) and hemoglobin A1c(HbA1c) were observed in T2DM and NGT group before and after treatment. The levels of FT3, FT4,TSH,HOMAIR and HbA1c were observed in T2DM group with different HbA1c levels, and the correlation between thyroid hormone and HOMAIR were also observed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The levels of FT3 and FT4 in T2DM group were significantly lower than those in NGT group, and the levels of TSH, HOMAIR and HbA1c were significantly higher than those in NGT group(P＜005). The FT3 and FT4 levels decreased with the increase of HbA1c level. And the levels of TSH, HOMAIR in T2DM group increased with the increase of HbA1c level(P＜005). After treatment, the levels of FT3 and FT4 were significantly increased compared with before treatment, while, the levels of TSH, HOMAIR and HbA1c were significantly lower compared with those before treatment(P＜005). The levels of FT3 and FT4 were negatively correlated to the levels of HOMAIR(r=-0.781, -0.564, P＜005), and there were positively correlated between TSH and HOMAIR levels(r=0.648, P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓There are thyroid axis micro disorder and insulin resistance in patients with T2DM and obesity, and thyroid axis micro disorder is correlated significantly to insulin resistance. Treatment of T2DM is helpful in alleviating the thyroid dysfunction and insulin resistance.


Effects of resveratrol on biological behavior of human esophageal cancer cell EC9706 and its mechanism

2017, 37(1):  11-15,19.  doi:10.3969/j.issn.10073205.2017.01.003

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［Abstract］ Objective〖HTSS〗〓To explore the effects of resveratrol on biological behavior of human esophageal cancer cell EC9706 and its mechanism, and to provide a theoretical basis for the clinical application of resveratrol to prevent or treat esophageal cancer.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Different concentrations of resveratrol were used to treat EC9706 cells for different time. Cell morphology was observed by inverted phase contrast microscope. Intracellular micromorphological changes were observed under electron microscope. Methylthiazoliltetracolium colorimetric enzyme reactions trace method were used to determinate cell proliferation level. Flow cytometry method was used to detect cell cycle distribution and the expression of apoptosis related protein.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Resveratrol treatment increased and thickened particles in EC9706 cytoplasm, reduced the cytoplasm, rounded cells and made cells more smaller and the cell shape more diversity, and made the nuclear chromatin condense on the boundary of the nuclear envelope. The electron density was increased, part of the cell membrane bulge was outward and form an acute angle. There was vacuoles degeneration in the cytoplasm. Resveratrol inhibited EC9706 cell proliferation in a dose and timedependent manner. Resveratrol made subdiploid nuclear peak(apoptotic peak) appear before G0/G1  phase in EC9706 cells, and increased the rate of apoptosis together with proportion of G0/G1 phase. In contrast, resveratrol decreased the S and G2/M phase fraction(compared with the control group, P＜001). Resveratrol treatment also decreased expression of bcl2 protein, and increased  expression of bax protein(compared with the control group, P＜001).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Resveratrol can inhibit the proliferation and induces apoptosis of EC9706 cells, and make the cell cycle arrest in G0/G1 phase. This effect may be related to apoptosis gene bcl2 and bax.


Clinical study of hemodynamic monitoring in patients with septic shock by bedside ultrasound

ZHANG Bo, DING Kaifang, YANG Dongxing, GU Qing

2017, 37(1):  16-19.  doi:10.3969/j.issn.10073205.2017.01.004

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［Abstract］Objective〖HTSS〗〓To evaluate the value of bedside ultrasound in patients with septic shock hemodynamic monitoring.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Eightysix cases shock patients in ICU were randomly divided into two groups:the control group using pulse indicating continuous cardiac output(PICCO) monitoring technology, monitoring indicators to guide patients to rehydration treatment, observation group using bedside ultrasound monitoring indicators to guide patients to rehydration treatment. The hemodynamics parameters measured by different measurement mode and the treatment effect as well as prognosis of different guiding methods were compared.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓There was no difference in HR, CVP, MAP, blood lactate levels, urine output, vasoactive drugs score, APACHEⅡscore after treatment 6 h between two groups(P＞005). There was no significant difference in CI, CO and SV between the 2 groups before and after treatment(P＞005). There was no difference in 6h infusion volume, the amounts of dobutamine and norepinephrine in two groups(P＞005). After ICU treatment, SV, CO and CI in the 2 groups were significantly higher than those before entering the ICU(P＜005), but there was no significant difference between the 2 groups(P＞005). There was no difference in mechanical ventilation, ICU stay time in two groups(P＞005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Bedside ultrasound is convenient, noninvasive and realtime monitoring, which can be used for realtime monitoring and prediction of fluid resuscitation in septic shock patients, and has good application value.


Association of vitamin D receptor gene Apa1 and Taq1 polymorphisms with susceptibility to lung squamous cell carcinoma

YANG Yanhong1， WU Jiandong1， ZHOU Wenwen1， HOU Yanyan1, YIN Duanduan,WANG Zhenzhen2

2017, 37(1):  20-23,71.  doi:10.3969/j.issn.10073205.2017.01.005

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［Abstract］ Objective〖HTSS〗〓To investigate the relationship between polymorphism of vitamin D receptor（VDR） gene(including Apa1, Taq1) and susceptibility of squamous cell carcinoma of lung cancer(SCC).
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The blood samples were collected from 288 patients with SCC and 284 healthy individuals. Leukocyte DNA from peripheral blood was extracted. The method of polymerase chain reactionrestriction fragment length polymorphism(PCRRFLP) was applied to test the genotype of Apa1, Taq1 in VDR gene.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓VDR Taq1 gene polymorphisms is the risk factor of SCC. The SCC risk of tt+Tt genotype carriers is 0.556 times than the TT genotype carriers(OR=0.556， 95%CI=0.393-0.785). Based on stratification analysis of smoking: In smoking group, there is statistical significance in Taq1 gene polymorphisms. The SCC risk of tt+Tt genotype carriers is 0.049 times than the TT genotype carriers(OR=0.049， 95%CI=0.259-0.929). There is statistical significance in t allele and T allele. The SCC risk of t allele carriers is 0.444 times than the T allele carriers(OR=0.444， 95%CI=0.241-0.817).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Taq1 polymorphism is related to genetic susceptibility of SCC. The tt+Tt gene reduces the risk of SCC. The t allele is negatively correlated with the risk of SCC. Smokers with tt+Tt gene are significantly reduced the risk of SCC.


Investigation of four different posterior operative ways on curvature index and axial symptom for multilevel cervical spondylotic myelopathy

LI Pengfei, JIA Nan*, WANG Lihong, JIN Xianhui, LI Jiacheng, YANG Jianbo

2017, 37(1):  24-28.  doi:10.3969/j.issn.10073205.2017.01.006

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［Abstract］Objective〖HTSS〗〓To investigate the clinical significance of four different posterior operative ways on cervical curvature index(CCI) and axial symptom(AS) in the treatment of multilevel cervical spondylotic myelopathy(MCSM).
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓One hundred and thirtyfour patients with MCSM were randomly divided into four groups in this study. 34 patients underwent traditional laminectomy， 35 patients underwent opendoor laminectomy， 33 patients underwent lateral mass screw fixation， 32 patients underwent cervical pedicle screw fixation. All the patients were examined with Xray, CT and MRI. The operation time, bleeding volume, postoperative drainage volume, recovery time, nerve function before and after operation, CCI and AS were compared in 4 groups.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓There was no significant difference in operation time, bleeding volume, postoperative drainage volume and recovery time among 4 groups(P＞005) . There was no difference in Japanese Orthopaedic Association score recovery rate among 4 groups(P＞005). The CCI loss rate of opendoor laminectomy group, lateral mass screw group and pedicle screw group were less than that of traditional laminectomy group, the CCI loss rate of lateral mass screw group and pedicle screw group was less than that of opendoor laminectomy group(P＜005) . The intervertebral height of lateral mass screw group and pedicle screw group was lost less than that of traditional laminectomy group and opendoor laminectomy group(P＜005), There was no significant difference between the 4 groups in the axial symptoms and the distribution of AS(P＞005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Lateral mass screw and cervical pedicle screw fixation made a good clinical outcome. Although there was no significant effect on the postoperative recovery rate, the stability of cervical spine was significantly increased. They can effectively prevent postoperative curvature loss and reduce the occurrence of serious AS.


Effects of tranexamic acid on lumbar surgery by different ways of administration

MENG Xianyong, HU Changbo, YANG Xinming

2017, 37(1):  29-32,37.  doi:10.3969/j.issn.10073205.2017.01.007

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［Abstract］ Objective〖HTSS〗〓Use of tranexamic acid can reduce perioperative blood loss and decrease the rate of transfusion in the intraoperative and postoperative lumbar spine surgery. To analysis the effects of tranexamic acid on lumbar spine surgery blood loss and transfusion rate by comparing differences among the preoperative intravenous application group, simple application in the wound group and blank control group.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 120 patients performed lumbar posterior more than triarticular open operation were divided into 3 groups randomly, 40 patients in each group. Patients with topical application of tranexamic acid in the wound among surgery were belong to group A, patients with intravenous application of tranexamic acid at half an hour before surgery were belong to group B, and patients with no tranexamic acid were belong to group C. Some indicators will be observed, such as time of operation, intraoperative blood loss, postoperative blood loss in 48 hours, condition of the blood transfusion and complication, etc.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The postoperative incisions of patients in 3 groups were primary healing. There were no significant differences in 3 groups about the time of operation（P＞005）. The amount of postoperative blood loss in 48 hours in group A and B was less than it in group C（P＜005）.Compared to group C, the per capita amount of blood transfusion in group A and B was decreased（P＜005）, and the amount in group A was less than it in group B（P＜005）. The number of patients with transfusion in group A and B was less than it in group C, and the difference was statistically significant（P＜005）.There were no significant differences in the incidence of complications among 3 groups（P＞005）.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓In the lumbar spine surgery, tranexamic acid can reduced the hidden blood loss, intraoperative and postoperative blood loss and blood transfusion rate.


Effects of ovarian endometriosis cyst with no surgical treatment on the outcome in vitro fertilization and embryo transfer

LI Mingting1, YAN Xue2, CAO Mingya3, ZHAO Zhiming3*

2017, 37(1):  33-37.  doi:10.3969/j.issn.10073205.2017.01.008

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［Abstract］Objective〖HTSS〗〓To investigate the ovarian response, embryo quality and others outcomes of patients with ovarian endometriosis cyst and no surgery performed treatment before in vitro fertilizationembryo transfer(IVFET).
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The clinical data in infertility women of ovarian endometriosis cyst with no surgery treatment performed in reproductive medicine center in the second hospital of Hebei medical university undergoing IVFET were performed by retrospective analysis. Fiftyeight cases of ovarian endometriosis cyst with no surgical treatment was used as endometriosis(EM) group, with the same period 55 cases of tubal factor in IVF was used as control group. GnRHa super long protocol was used for the ovarian stimulation. Clinical indexes(such as ovarian response to gonadotropin, number of oocytes), laboratory indexes(such as number of good quality embryos) and IVF pregnancy outcome were observed and compared between the EM group and the control group.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓There were no significant difference in the mean age, the duration of infertility, the base follicle stimulating hormone level, the duration of goadotropins, the goadotropins consumption, the number of transferred embryos between the EM group and control group(P＞005). The serum estradiol level on the human chorionic gonadotropin injection day, the number of retrieval oocytes in EM group, were lower than those in the control group, and the differences were statistically significant(P＜005). In the EM group the fertilization rate, the high quality embryo rate were lower than those in the control group, the difference was statistically significant(P＜005). The clinical pregnancy rate in the EM group was lower than that in the control group, but there was no statistically significant difference.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Endometriosis cyst may have certain negative effects of ovarian response and embryo quality in IVF. It may reduce the number of retrieval oocytes and good quality embryos, which could eventually affect pregnancy outcomes.


Clinical efficacy of Ganciclovir combined Qing Wen decoction on the treatment of EB infection in children and its influence on immune function

QI Jianping1， QI Xiaoyuan2， WANG Xiaojuan3

2017, 37(1):  38-42.  doi:10.3969/j.issn.10073205.2017.01.009

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［Abstract］ Objective〖HTSS〗〓To observe the clinical effect of the Qing Wen decoction combined with ganciclovir on the treatment of children with EBV infection, and to analyze its influence on immune function, provide the basis for the treatment of EB virus infection with integrated traditional Chinese and Western Medicine.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Eighty cases of children with EB infection were selected and randomly divided into the observation group and the control group, 40 cases in each group. The control group patients were treated with ganciclovir, the observation group patients were treated by Qing Wen decoction combined with ganciclovir, treatment effects were compared between the two groups.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The total effective rate of the observation group and the control group were 95%，80%, and the difference in the effective rate between the two groups was statistically significant (P＜005). Compared with the control group, the improvement time of the observation group was significantly shorter (P＜005). Children EBVNAIgG negative before and after the treatment had no significant change (P＞005), but compared with before treatment, the number of EBVDNA and EBVCAIgM negative cases of the two groups of children significantly increased after treatment (P＜005), and the observation group increased the number of cases was significantly higher than that of control group (P＜005). Compared with before treatment, ALT, AST and immune globulin in two groups were decreased significantly after treatment, and the decreased degree in observation group was higher than that in the control group; lymphocyte subclass was improved significantly after treatment in two groups of children, and the improvement degree of the observation group was higher than that of the control group (P＜005). The adverse reaction rates in the two groups were not statistically significant (P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The effect of Qing Wen decoction combined with ganciclovir in treatment of children with EB infection is curative. Symptoms subside quickly in children. The drugs have less adverse reaction, can effectively reduce the EBDNA content, and promote the EB virus transformation. It is important to the reduce the risk of children infected after the occurrence of malignant disease.


Expression of CEACAM l and VEGF in mucoepidermoid carcinoma of salivary glands and its effect on angiogenesis

MA Sai, AN Feng*, XU Aiwen, WANG Qin, ZHANG Li, LI Liheng

2017, 37(1):  43-47.  doi:10.3969/j.issn.10073205.2017.01.010

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Abstract］Objective〖HTSS〗〓To investigate the relationship of carcinoembryonic antigen related cell adhesion molecule 1 (CEACAM1) and vascular endothelial growth factors (VEGF) expression with angiogenesis in mucoepidermoid carcinoma(MEC) of salivary glands.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The expression of CEACAM1 and VEGF was detected with immunohistochemcal method streptavidinperosidase (SP) in 70 cases of MEC tissues，and 20 cases of normal salivary gland，the microvessel density(MVD) was recorded under optical microscope.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The positive expressions of CEACAM1 protein (34.3% vs 70.0%) was lower in MEC group than those in normal salivary gland group(P＜005)，the positive expressions of  VEGF protein (71.4% vs10.0%) was higher in MEC group than those in  normal salivary gland group(P＜005). In MEC group，the positive expressions of CEACAM1 protein in pathological grade levelⅢ patients were lower than those in pathological grade level Ⅰ+Ⅱ patients(P＜005)，the positive expressions of CEACAM1 protein in TNM stage Ⅲ+Ⅳpatients were lower than those in TNM stageⅠ+Ⅱ patients(P＜005)，the positive expressions of CEACAM1 protein in patients with lymph node metastasis were lower than that in patients without lymph node metastasis(P＜005). The positive expressions of VEGF protein and MVD in pathological grade level Ⅲpatients were higher than those in pathological grade level Ⅰ+Ⅱpatients(P＜005)，the positive expressions of VEGF protein and MVD in TNM stage Ⅲ+Ⅳ patients were higher than those in TNM stage Ⅰ+Ⅱpatients(P＜005)，the positive expressions of VEGF protein and MVD in patients with lymph node metastasis were higher than that in patients without lymph node metastasis(P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Downregulationg of CEACAM1 in MEC of salivary glands may play a role in angiogenesis through increasing the expression of VEGF，thus promote tumor invasion and metastasis.


Research using shear wave velocity of thyroid tissues among patients with graves disease and hashimoto′s thyroiditis

TIAN Hui1, CAO Yanmei2, ZHANG Yuena1， JU Hongjuan1，XU Wensheng1， ZHANG Tongdi1

2017, 37(1):  48-50,55.  doi:10.3969/j.issn.10073205.2017.01.011

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［Abstract］Objective〖HTSS〗〓To investigate the changes of the shear wave velocities（SWV） of thyroid tissues among patients with Graves disease(GD)and Hashimoto's thyroiditis(HT) by using Shear Wave velocity.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓 Fortyfive cases who were diagnosed with initial GD and 37 cases who were diagnosed HT and 50 healthy volunteers as control group were included in the study. The fasting blood samples were taken from every case in the morning，the thyroid function and related antibody were detected. SWV of thyroid tissues was measured by the same doctor. SWV was compared between GD group，HT group and control group. The correlation between thyrotropin，thyroid stimulating hormone(TSH)， free triiodothyronine(FT3)， free thyroxine(FT4) levels and SWV was analyzed.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Thyroid artery flow velocity in GD group is higher than the HT group, the difference was statistically significant（P＜005）. But there was no statistical difference in color flow grade(P＞005). Thyroid volumes of cases in GD group were significantly higher than those of cases in HT and control group（P＜005）. SWV values in GD and HT group are higher than the control group(P＜005). No correlation was detected between thyroid function and SWV value in GD and HT group(P＞005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓SWE imaging can be objective and quantitative assessment thyroid tissue, but it is difficult to distinguish patients with GD and HT. Marked increased volume and the flow in upper thyroid artery both play a certain value in differential GD and HT.


Correlation analysis of EEG and serum levels of inflammatory factors in children with idiopathic epilepsy

HAN Jie1, PING Lili2, HU Limin3, HOU Xiaoying1

2017, 37(1):  51-55.  doi:10.3969/j.issn.10073205.2017.01.012

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［Abstract］ Objective〖HTSS〗〓To analyze the relevance of EEG in children with idiopathic epilepsy to serum levels of inflammatory factors.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Seventyfive cases diagnosed with idiopathic epilepsy included in the study were divided into 3 groups according to the frequency of epileptic seizures: epilepticus status group, nonepileptic frequent or persistent state group, and persistent state group, which included 25 cases in each group. Thirty healthy children were enrolled as control cases. Serum level of inflammatory factors (HMGB1, IL6, IL2, TNFα) and EEG findings were analyzed in both epilepsy and control groups.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Serum level of HMGB1, IL2, IL6, TNFα in in cases with epilepsy were significantly higher than that in control group (P＜005), serum level of HMGB1, IL2, IL6, TNFα in cases with epilepticus status and frequent epilepsy were both higher than that in nonfrequent seizures or persistent state group (P＜005); Serum level of HMGB1, IL2, IL6, TNFα in cases with EEG epileptic discharge and  slow waves were significantly higher than those with normal EEG and boundary resistance (P＜005). Serum level of HMGB1, IL2, IL6, TNFα levels in cases with EEG epileptic discharge were significantly higher than those with EEG slow waves (P＜005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓HMGB1, IL2, IL6, TNFα are important inflammatory factors relevance to idiopathic epilepsy. Abnormalities findings in EEG were positively correlated with serum levels of HMGB1, IL2, IL6, TNFα.


Study on clinical application of abdominal ultrasound and transvaginal ultrasound in the diagnosis of gynecological acute abdomen

HAN Peichen1, CHEN Wei2*

2017, 37(1):  56-58.  doi:10.3969/j.issn.10073205.2017.01.013

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［Abstract］ Objective〖HTSS〗〓To study the value of transabdominal and transvaginal ultrasound in the diagnosis of gynecological acute abdomen.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 168 patients with gynecological acute abdomen confirmed by surgery was randomly assigned to transabdominal  ultrasound group (n=56), transvaginal ultrasound group (n=56), and the combined approach group (n=56). The ultrasound results were compared with clinical diagnostic results.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The results showed that the accuracy of the combined ultrasound diagnosis was 94.6%, the accuracy rate of abdominal ultrasound and transvaginal ultrasound were 73.2% and 82.1%, respectively. The diagnostic accuracy of combined ultrasound diagnosis group was significantly different from that of the 2 groups (P＜005). Coincidental rate of transabdominal combined with transvaginal ultrasound was significantly higher than that alone use, there was significant difference between them.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Abdominal ultrasound combined with transvaginal ultrasound in the diagnosis of gynecological acute abdomen is superior to the single application of abdominal ultrasound or transvaginal ultrasound. The diagnostic accuracy is obviously improved, and it is worth popularizing in clinic.


Analysis on pollution of central air conditioning and ventilation system in public places in Hebei in 2015

GUO Feifei1, MA Zhe2, YANG Feng2, YANG Hui1, NIU Yujie1*

2017, 37(1):  62-65.  doi:10.3969/j.issn.10073205.2017.01.015

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［Abstract］Objective〖HTSS〗〓To learn the hygiene status of central air conditioning ventilation system in Hebei province and find the existing problems in hygiene detection in public places.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 90 central air conditioning ventilation systems in public places were sampled. Legionella pneumophila in cooling water, hemolytic streptococcus in the supply air, accumulated dust, total bacteria, and total fungi on the internal surface of ventilation ducts were detected.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Legionella pneumophila was not detected in cooling water  but the detection rate of hemolytic streptococcus in the supply air was 13.3%. The qualified rates of accumulated dust, total bacteria, and total fungi on the internal surface of ventilation ducts were 96.4%, 95.5% and 99.2%, respectively.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓There are some problems in the health status of centralized air conditioning ventilation system in public places in Hebei province, wherein hemolytic streptococcus contamination severe.


Study on the compatible stability of Azithromycin Lactobionate and Budesonide #br#

REN Jinmin1, SHEN Jun2, SUN Li1, MA Jiangman1, CUI Xiaohong1

2017, 37(1):  66-71.  doi:10.3969/j.issn.10073205.2017.01.016

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［Abstract］  Objective〖HTSS〗〓To investigate the compatible stability of Azithromycin Lactobionate for injection with budesonide and ambroxol hydrochloride for injection.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓Basing on clinical medication methods, azithromycin lactobionate, budesonide, ambroxol hydrochloride were prepared to compatibility solution with 0.9% sodium chloride injection. The contents of three drugs within 7 days were determined by liquid chromatographymass spectrometry/mass spectrometry（LCMS/MS）. The appearance and pH value of mixture were investigated.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The contents of azithromycin lactobionate, budesonide and ambroxol hydrochloride changed less than 5% within 2 days, and the contents of three drugs was equal to 70%-90% of 0 h at 3 days, the contents of three drugs decreased significantly after 3 days, only less than 50% of 0 h. When azithromycin lactobionate and budesonide were put in different compatible solutions and stored at different temperatures, the concentration of azithromycin and budesonide were decreased. There was statistical significance difference between time points(P＜005), but there was no significant difference between groups(P＞005), and the interaction between groups and time(P＞005).
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓Azithromycin lactobionate, budesonide and ambroxol hydrochloride are compatible, should be preserved at room temperature, and should not exceed 3 days for nose drops use.


Analysis on the relationship between amount of antibiotics and antimicrobial resistance of acinetobacter baumannii in our hospital

FENG Rui, ZHENG Ying, GAO Yaqian, LI Dong, LIU Jiang, DU Wenli*

2017, 37(1):  72-75.  doi:10.3969/j.issn.10073205.2017.01.017

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［Abstract］Objective〖HTSS〗〓To investigate the drug resistance status and trends of acinetobacter baumannii from our hospitalized patients between January 2013 and June 2015 so as to provide references for guiding reasonable application of antibiotics clinically.
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓The drug resistance rates of acinetobacter baumannii and defined daily dose(DDDs) used eight kinds of antimicrobial drug were retrospectively analyzed. Meanwhile, nonlinear relationship was estimated by regression.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓Acinetobacter baumannii has a high resistance to a variety of commonly used antimicrobial agents. It is significantly positively correlated with the consumption of meropenem(r=0.667, P=0.035) and negatively correlated with the consumption of cefepime(r=-0.736,P=0.015). There was no correlation between the resistance rate of acinetobacter baumannii and DDDs of piperacillin sodium/he azole sodium, imipenem/cilastatin, cefoperazone/sulbactam, ceftazidime, levofloxacin, ciprofloxacin（P＞005）. 
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓There is a certain correlation between the use of antibacterial drugs and the rate of bacterial resistance. Antibiotics should be used rationally in accordance with clinical sensitivity test results as far as possible. The cefoperazone/sulbactam and cefepime are the better options to treat acinetobacter baumannii infection in our hospital.