Abstract: ［Abstract］ Objective〖HTSS〗〓To evaluate the correlation between physical status of human papillomavirus 16（HPV16） and degree of cervical lesion and the role of HPV16 integration in prediction and diagnosis of high squmous intrepithelil lesion(HSIL).
〖HTH〗〖WTHZ〗Methods〖HTSS〗〓A total of 165 cervical cells samples were collected from the patients with HPV positive. All patients′ pathological results were definite, including 18 cases of cervicitis, 24 cases with cervical intraepithelial neoplasia（CIN）1, 45 cases with CIN2, 51 cases with CIN3 and 27 cases with cervical cancer. The total genomic DNA were extracted and multiplex PCR were performed for detection of the level of HPV E2 and E6 genes. Comparing the physical status of HPV16 in different degrees of cervical lesions according to the E2/E6 ratio.
〖HTH〗〖WTHZ〗Results〖HTSS〗〓The single infection ratios of HPV16 in cervicitis, CIN1, CIN2, CIN3 and cervical cancer were 6.73%(7/104), 10.58%(11/104), 29.81%(31/104), 30.77%(32/104), 22.11%(23/104), respectively. Single HPV infection rate was gradually increasing with the processing of cervical lesion level. The integration ratios of HPV16 in cervicitis, CIN1, CIN2, CIN3 and cervical cancer were 16.67%(3/18), 20.83%(5/24), 46.67%(21/45), 62.74%(32/51), 77.78%(21/27), respectively. The difference was statistically significant(P<005). The sensitivity and specificity of diagnosis of cervical lesions by HPV16 DNA integrate status were 60.16% and 79.07% and the positive predictive value and negative predictive value were 40.96% and 90.24%.
〖HTH〗〖WTHZ〗Conclusion〖HTSS〗〓The E2/E6 ratio of HPV16  can be used as indicators to judge the state of viral integration, and the integration status of HPV16 was accompanied with the processing of cervical lesion level.

Key words: uterine cervical neoplasms, cervical intraepithelial neoplasia, human papillomavirus 16