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    25 November 2016, Volume 37 Issue 11
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    doi:10.3969/j.issn.10073205.2016.11.001
    LI Yukun1, WANG Rongying1*, TIAN Yingping2, HE Zhenyin1
    2016, 37(11):  1241-1245,1249. 
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    [
    Abstract ] Objective Toevaluatethediagnosticandprognosticperformanceofheart-type
    fattyacidbindingprotein ( H-FABP ) intheearlyofacutecoronarysyndrome ( ACS ) .Methods A
    prospectivestudywasusedin173patientswithchestpainwithin12h.H-FABP , cardiactroponin
    I ( cTnI ) andmyoglobin ( MYO ), creatinekinase ( CK ), creatinekinase-MB ( CK-MB ) andother
    biochemicalindicatorsweremeasured.Theincidenceofmajoradversecardiacevents ( MACE ) was
    observedinpatientsduringhospitalization.Therelevantinformationwascollectedandanalized.
    Results ①ThepositiverateofH-FABPinacutemyocardialinfarction ( AMI ) wassignificantly
    higherthancTnI , MYO , CKandCK-MBinthechestpainonset0-3h ( P <0.05 ) .The sensitivity , positivepredictivevalue
    (
    PPV ), accuracyandYoudenindexofH-FABPwerehigher
    thancTnI , MYO , CK , CK-MB.②ThepositiverateofH-FABPinAMIwassimilarwithcTnI
    andMYO ( P >0.05 ), butsignificantlyhigherthanCK , CK-MB ( P <0.05 ) in>3-6hours.The
    positivepredictivevalue , accuracyandYoudenindexofH-FABPweresimilarwithcTnI , but
    higherthan MYO , CK , CK-MB.③ThepositiverateandsensitivityofH-FABPinAMIwas
    similarwithcTnI ( P >0.05 ) in >6-12hours.Thepositivepredictivevalue , accuracyand
    YoudenindexofH-FABPweresimilarwithcTnI , CK , CK-MB , buthigherthanMYO.④The
    specificityofH-FABP wassimilarwithcTnI , buthigherthan MYO , CK , CK-MBin0-12
    hours.⑤ThediagnosticvalueofH-FABPwashigherthanotherindexesin0-3hours.⑥The
    negativepredictivevalue ( NPV ) ofH-FABPandH-FABP+cTnIwerehigherthancTnI , MYO ,
    CK , CK-MB.⑦Accordingtotheincidenceofmajoradversecardiacevents ( MACE ) ingroup
    AMIinaweek , the OR ofH-FABPis2.761 , 95% CI is0. 704-0.902.Conclusion ①The
    sensitivity , accuracyandspecificityofH-FABPishigherthancTnI
    , MYO , CK , andCK-MB.
    ThereforeH-FABPcanbeusedasasensitiveindicatorforearly AMI , particularly within3
    hours.②cTnI+H-FABPsubstantiallyenhancetheNPVofcTnIwithin6hours , anditcanbe
    usedforexcludingAMIwithasingleblooddrawatpre
    Clinicalstudyofhyperbaricoxygencombinedwithpregabalininthetreatmentof#br# painfuldiabeticperipheralneuropathy
    LIU Xianhong1, JIN Yanxia1, NIU Jinbao2, FENG Huihui3, WU Shaohua4, SUN Xiuquan5
    2016, 37(11):  1246-1249.  doi:10.3969/j.issn.10073205.2016.11.002
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    [
    Abstract ] Objective Toobservethetherapeuticeffectofhyperbaricoxygencombinedwith
    pregabalininthetreatmentofpainfuldiabeticperipheralneuropathy.Methods Onehundredand
    sixpatients withdiabeticperipheralneuropathy weredividedintothreegroups : hyperbaric
    oxygengroup ( Agroup ), pregabalin (
    Bgroup ), hyperbaricoxygencombinedwithpregabalin (
    C
    group ), 35casesinAgroup , 35casesinBGroup , 36casesinC Group.Clinicaleffiencyand
    electomyogram withcondutionvelocityofmedianandperoneuscommonnervewerecompared
    after5 weeks.Results ClinnicaleffiencyingroupC was83.3% aftertreatment , which was
    higherremarkablelythan group A ( 57.1% ) .There weresatisticaldifference ( P <0.05 ) .
    CondutionvelocityofmedianandperoneuscommonnerveinCgroupwerehigherremarkablely thangroupAandB , thereweresatisticaldifference ( P <0.05 ) .Conclusion Hyperbaricoxygen
    combinedwithpregabalinintreatingpainfuldiabeticperipheralneuropathy waseffectiveand
    safety , whichprovidesaneweffectivemethodforthetreatmentofdiabeticneuropathy
    Studies on the acute and chronic toxicity test of dl3nbutylphthalide in mice
    TIAN Xiaochao1, HE Weiliang2, LIU Siwen3, Yang Rong1, LIU Suyun1*
    2016, 37(11):  1250-1252,1256.  doi:10.3969/j.issn.10073205.2016.11.003
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    [ Abstract ] Objective To explore the acute toxicity and chronictoxicity of dl-3-n-
    butylphthalide ( NBP ) inmice.Methods TheBlissmethodwasusedtoobservetheacutetoxic
    symptomsandtoobtainthetoxicparametersmediallethaldose ( LD50 ) ofNBPinmice.Repeated
    administrationoftailveinfor30days , andthelong-termtoxicitytomicewasobserved.NBPis
    dividedintolow , mediumandhighdosegroup ( 40 , 80and160mg / kgrespectvely ), normalsaline
    wasusedascontrolgroup.Thechangesofthebloodchemistry ( alaninetransaminase , aspartate
    aminotransferase , bloodureanitrogen , serumcreatinine ), andhistopathologywereexamined.
    Results TheLD50valueoftheNBPwas545. 1mg / kg.Theelevatingtendencyofthelevelsof
    aspartateaminotransferasewasobservedinthehighdosegroupofNBP.Nosignificantchanges
    wereobservedinclinicalsigns , otherbloodchemistry , andhistopathology.Conclusion The
    rationaluseofNBPwillnothaveadverseeffectsonthetargetanimal.
    Efficacy of idebenone in the treatment of cognitive impairment inchronic schizophrenia
    XU Xiaomei, HAO Cuijie， SHI Yumei, ZHAO Yujun, ZHU Lin, NIU Weifang
    2016, 37(11):  1253-1256.  doi:10.3969/j.issn.10073205.2016.11.004
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    [
    Abstract ] Objective Toinvestigatetheefficacy and safety ofidebenone on chronic
    schizophreniapatientswithcognitivedysfunction.Methods Onehundredandfourcasesofmale
    chronichospitalschizophreniawererandomlydividedintostudygroup ( n =52 ) andcontrolgroup
    (
    n =52 ) for12weeks.Studygroupwastreatedwithantipsychoticsandidebenone , andcontrol
    groupwastreatedwithantipsychoticsandplacebo.Beforeand2 , 4 , 8 , 12weeksaftertreatment ,
    repeatablebatteryforneuropsychologicalstatus ( RBANS ) wasusedtoevaluatethecognitive
    function , andpositiveandnegativesyndromescale ( PANSS ) andtreatmentemergentsymptom
    scale ( TESS ) wereusedtoevaluatetheclinicalefficacyandsideeffectsrespectively.Results
    Patientsinthestudygroupweremoreeffectiveinthecontrolofthedisease ( P <0.05 ) .After
    treatmentRBANSscoresinthe2groupsofpatientsweregraduallyincreased , PANSSscores
    weredecreasedgradually.ThedifferencesofRBANSscoreandPANSSscoreofthe2groupshave
    statisticalsignificanceinthegroups , inthetimepoint , intheinteractionofgroupandthepoint
    (
    P <0.05 ) .Therewasnosignificantdifferenceinadversereactionratesbetweenthe2groups
    (
    P >0.05 ) .Conclusion Idebenoneoncognitioninpatientswithchronicmentaldisordersissafe
    andeffective , andcanbeusedinclinic.
    Clinical efficacy and changes of serum leptin levels in patients with simple obesity and fatty liver treated by Qianggan capsule
    LIU Hongfen1, FAN Yakun2, ZHANG Shumei1, CHANG Lili1
    2016, 37(11):  1257-1259,1269.  doi:10.3969/j.issn.10073205.2016.11.005
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    [
    Abstract ] Objective Toobserveclinicalefficacyandchangesofserumleptinlevelsin
    patientswithsimpleobesityandfattylivertreatedbyQianggancapsule.Methods Sixty-two
    patientswererandomlydividedintocontrolgroup ( 30cases ) andQianggancapsulegroup ( 32
    cases ) .Twogroupshadstrictdrinking , appropriatereduceenergyperturbationandincreasedthe
    workout.ThetreatmentgroupwasgivenQianggancapsule2. 0gorally , 2times / day , Whilethe
    treatmentgroupwasgivenatorvastatin20 mgorally , 1time
    / day.Thetreatmentcoursesof2
    groupswere6 months.Results Themoreseriousthedegreeofobesity , thelessofliverand
    spleenCTratio.Thedifferencewasstatisticallysignificant ( P <0.05 ) .Thereweresignificant
    differencesinthe2groupsaftertreatmentthanbeforetreatment ( P <0.05 ) .Aftertreatment
    BMI , liverandspleen CTratio , ALT , AST , γ-GT , TC , andleptinofthe2groups were
    statisticallysignificant ( P <0.05 ) .ThedifferenceofwaisttohipratioandTGin2groupsafter
    treatmentwasnotstatisticallysignificant ( P >0.05 ) .Theclinicalefficacyandtotaleffectiverate
    ofthe2groups werestatisticallysignificant ( P <0.05 ) .Conclusion Theclinicaleffectof
    Qianggancapsuleonobesefattyliverwassignificant.
    Influence of life style and nutritional status on the pain among gerontal patients with osteoporosis
    XIONG Mingshun1, LIU Xiaoping2, MENG Yujie1, ZHAO Xiujuan2, CHEN Changxiang3
    2016, 37(11):  1260-1263.  doi:10.3969/j.issn.10073205.2016.11.006
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    [
    Abstract ] Objective Tounderstandtheinfluenceoflifestyleandnutritionalstatustothe
    painamonggerontalpatientswithosteoporosis , andtomakeiteasytotakesomeintervention
    clinicallyforlessincidencerateofpain.Methods Questionnaireswerecarriedoutamonggerontal
    patientswithosteoporosisaboutthelifestyleandnutritionalstatus , meanwhilebynumerical
    ratingscaleforthelevelofpain , andbymininutritionalassessmentfortheirnutritionalstate.
    Results Theincidenceofpainin208patientswas81. 3% , andfemaleovermale.Thepainscore
    ofosteoporosispatientswithgoodnutrition waslowerthan who withgeneralnutritionand
    malnutrition.Themarriedpainscorewaslowerthanthewidowed.Thepainscoreofosteoporosis
    patientslivingincouplewaslowerthanthoselivingaloneandlivingwithdescendents ( P <0.05 ) .
    Thepainscoreofregularandoccasionalexercisepatientswaslowerthanthatofthenever
    exercisepatients ( P <0.05 ) .Thepainscoreofpatientswhohadonechildwaslowerthanwho
    hadmorechildren ( P <0.05 ) .Thepainscoreofoftenteadrinkerswaslowerthanthosewho
    neverdrinktea ( P <0.05 ) .Theoverallevaluationscoresofmoderateandseverepaingroupwere
    lowerthanthoseinthemildpaingroup ( P <0.05 ) .Thedietoftheseverepaingroupwaslower thanthatofthe moderatepaingroup.Thedifferencewasstatisticallysignificant ( P <0.05 ) .
    Conclusion Theincidencerateofpainishigheramonggerontalpatientswithosteoporosis.
    Maybeit'srelatedtothelifestyleandnutritionalstatusofthegerontalpatients.
    Clinical outcome of arthroscopic singlebundle posterior cruciate ligament reconstruction with Rigidfix and Intrafix
    ZHANG He， HAN Shoujiang*， WANG Bin， ZHANG Zhaojun， LI Jiangtao， GAO Xiaokang
    2016, 37(11):  1264-1269.  doi:10.3969/j.issn.10073205.2016.11.007
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    [
    Abstract ] Objective Toevaluatetheclinicaloutcomesofarthroscopicsingle-bundle
    posteriorcruciateligamentreconstructionusingRigidfixandIntrafix.Methods Totalsof46
    casesofPCLreconstructionwithRigidfixandIntrafixwererecordedintheretrospectivecohort
    study.Evaluationsincluded International Knee Documentation Committee ( IKDC ) score ,
    Lysholmscores , tegnerscores , andKT-2000inpostoperativefollowing-up.Results Totalsof46
    patientshadbeenfollowedupfor9to36 months ( average18 months ) .MagneticResonance
    Imaging ( MRI ) examinationshowedthatthePCLreconstructionrestorednormally.Allincisions
    wereprimaryhealingandjointwasstable.Allthepatientsrecoveredafullrangeofmotion
    withoutanysurgicalcomplications.TheIKDC , Lysholm , tegnerscorespostoperatively were
    higherthanthatofpre-operative ( P <0.05 ), andtheKT-2000decreasedobviouslyaftersurgery
    (
    P <0.05 ) .Conclusion UsingRigidfix-IntrafixforarthroscopicPCLreconstructionhadlotsof
    advantagesincluding simple procedur
    The expression and clinical significance of AMPKACC signaling pathway in papillary thyroid cancer
    YAN Li1, LI Qinghuai1, JI Hong1, SHEN Wei1, GUO Hao1, WANG Changquan2
    2016, 37(11):  1270-1274,1278.  doi:10.3969/j.issn.10073205.2016.11.008
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    [ Abstract ] Objective Toinvestigatetheexpression and significance of AMPK-ACC
    signalingpathwayinpapillarythyroidcancer ( PTC ) bystudyingtheexpressionofphosphorylated
    AMP-activatedproteinkinase ( pAMPK ) andphosphorylatedacetyl-CoAcarboxylase ( pACC ) in
    tissuesofPTCandtheirrelationstotheclinicopathologicalfeatures.Methods Theexpressionof
    pAMPKandpACCintissuesof60casesofPTCand20casesofnodulargoiterweredetectedby
    immunohistochemicaland westernblot , andtherelationship withage , gender , tumorsize ,
    bilateral , multifocal , capsularinvasion , lymphnode metastasis wasanalyzed.Results The
    expressionofpAMPKandpACCintissuesofPTCwassignificantlyhigherthanthatinnodular
    goitertissues ( P <0.05 ) .TheexpressionofpAMPKandpACCincentrallymphaticmetastasesof
    PTCwassignificantlyhigherthanthatinPTCwithoutcentrallymphaticmetastases ( P <0.05 ) .
    TheexpressionofpAMPKandpACCinlaterallymphaticmetastasesofPTCwassignificantly higherthanthatinPTCwithoutlaterallymphaticmetastases ( P <0.05 ) .Age , gender , tumor
    size , bilateralcarcinoma , multifocalandcapsularinvasionwerenotrelatedtotheexpressionof
    pAMPKandpACCin PTC ( P >0.05 ) .WesternblotdetectionshowedpAMPK andpACC
    expressionsinPTCtissueweresignificantlyhigherthaninnodulargoiter ( P <0.05 ) .Conclusion
    pAMPKandpACCaresignificantlyup-regulatedinPTC , andtheincreaseddegreewasclosely
    relatedtonecklymphnodemetastasis.Theac
    Evaluation of the value of combination of stage Ⅰ in laparoscopic surgery and ERCP in the elderly patients with gallbladder and bile duct stones
    CHEN Shengxiong, LIU Jianhua*， YAN Changqing， WANG Wenbin， ZHOU Zegao， QIN Jianzhang
    2016, 37(11):  1275-1278.  doi:10.3969/j.issn.10073205.2016.11.009
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    [
    Abstract ] Objective Toevaluatethevalueofcombinationofstage Ⅰ inlaparoscopic
    surgeryandendoscopicretrogradecholangiopancreatography ( ERCP ) intheelderlypatientswith
    gallbladder and bile duct stones.Methods Ninety-six cases of elderly patients with
    cholecystocholedocholithiasis , accordingtothedifferentsurgical methods , weredividedinto
    laparoscopiccholecystectomy+preoperativeencoscopicretrogradecholangiopancreatography ( 46
    cases ) and laparoscopic cholecystectomy + one-stage intraoperative encoscopic retrograde
    cholangiopancreatography ( 50cases ) .Theoperationtime , successfulrateofstoneremoval
    ,
    complicationandhospitalizationtime , patientcomfortindexandcostswerecomparedin2groups
    ofpatients.Results Theoperationsfortwogroupsofpatientsweresuccessful.Theaverage
    operationtimeofcombinationofstage ⅠinlaparoscopicsurgeryandERCP wasshorterthan
    laparoscopiccholecystectomy+preoperativeERCP , thedegreeofcomfortofpatientswashigher
    thanthatoflaparoscopiccholecystectomy+preoperativeERCPgroup , thehospitalizationtimeand
    totalhospitalizationcostswaslessthanlaparoscopiccholecystectomy+preoperativeencoscopic etrogradecholangiopancreatographygroup ( P <0.01 ) .Therewasnosignificantdifferenceinthe
    incidenceofcomplicationsbetweenthe2groups ( P >0.05 ) .Conclusion CombinationofstageⅠ
    inlaparoscopicsurgeryandERCPcanimprovepatientcomfort , shortenthehospitalizationtime ,
    reducethecostofsurgery , alsodoesnotincreasetheincidenceofcomplications , provide
    theoreticalandpracticalbasisforfurtherclinicalapplication , soastoprovidethepatientswith
    betterservice.
    Efficacyof1470nmlaservaporizationandtransurethralresectionof prostateforthetreatmentofbenignprostatichyperplasia
    TAN Wenchang1， HE Jingjing2， YU Xiaoming1
    2016, 37(11):  1279-1281,1301.  doi:10.3969/j.issn.10073205.2016.11.010
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    [
    Abstract ] Objective Tocomparetheefficacyandsafetyof1470nmlaservaporizationand
    transurethralresectionofprostate ( TURP ) forthetreatmentofbenignprostatichyperplasia.
    Methods Retrospectiveanalysisof108casesofprostatichyperplasiainourhospitalbythesame
    medicaldoctor ( TURP , n =54 ; 1470nm , n =54 ) .Operationtime , postoperativeretentiontime ,
    operationbleedingandinternationalprostatesymptomscore ( IPSS ), maximumurinaryflowrate
    ( Qmax ), residualurineimprovementinthe2groupswerecompared.Results 1470nmgroup
    hadlongeroperationtime , lessdurationofFoleyandlessdecreaseinhemoglobinthanTURP
    group ( P <0.05 ) . 1470nmgrouphadabetterimprovementofQmaxaftersurgery ( P <0.05 ) .
    TherewasnosignificantdifferenceintheimprovementofIPSSandresidualurine ( P >0.05 ), and
    postoperativecomplications ofthe 2 groups were notstatistically significant ( P >0.05 ) .
    Conclusion Application of1470 nm semiconductorlaservaporization ofprostateforthe
    treatmentofbenignprostatichyperplasiaissafeandeffective , andshowslessbleedingduring
    operation , shorterretentiontimeofthecatheter.Theimprovementofthemaximumurineflow
    rateaftershort-term operationisbetter.Buttheoperationtime waslongerthanthatof
    transurethralresectionoftheprostate.
    Analysis on the relationship between the psychological status and the pregnancy outcome of IVFET patients
    YANG Congpin, YANG Jing, LI Meixia, ZHAO Zhiming, LI Aimin*
    2016, 37(11):  1282-1284,1288.  doi:10.3969/j.issn.10073205.2016.11.011
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    [ Abstract ] Objective Toinvestigate the psychologicalchanges ofinfertile patients
    undergoinginvitrofertilization-embryotransfer ( IVF-ET ) andtoanalyzethepregnancyoutcome
    inordertoprovidebasisforfutureclinicalpractice.Methods EightypatientsforIVF-ET
    treatmentwerechosen.Accordingtothepregnancyoutcome , patientsweredividedintopregnant
    groupandnon-pregnantgroup , byusingpsychologicalscales , thestudy wastostudythe
    psychologicalcharacteristicsofthosepatientsduringthetreatmentprocess , includinginformation
    questionnaire , EysenckPersonalityQuestionnaire ( EPQ ), SymptomChecklist-90 (
    SCL90 ), Self-
    RatinganxietyScale ( SAS ) and Self-Rating Depression Scale ( SDS ) .Andthenanalyzedthe
    correlationbetweentheindexesofthetwogroups.Results Thereisnosignificantdifference
    (
    P >0.05 ) inE ( extroversion ) store , N ( neuroticism ) score , P ( psychoticism ) scoreandL ( lie )
    scorebetweenpregnantwomenandnon-pregnantwomen.Everyfactormarks , thetotalmarks
    andmeanmarksofSCL-90fornon-pregnantwomenwashigherthanthoseofpregnantwomen
    (
    P <0.05 ); thereisno differencein SASscorebetweenthetwo groups , SDSscoreof
    nonpregnantwomenwassignificantlyhigherthanthatofpregnantwomen ( P <0.05 ) .Conclusion
    Thereissignificantcorrelationbetweenthepsychologicalstateofthepatientandpregnancy outcome.Thisfindinghasguidingsignificancefornursing.Inordertoimprovethepregnancy
    rate , clinicaltreatmentshouldbecombinedwithpsychotherapy.Attentionshouldbepaidtothe
    impactofpatients'mentalhealthonpregnancyoutcomes
    Contentandclinicalsignificanceofinterleukin-6andtumornecrosis#br# factor-αofsalivainpatientswithrecurrentaphthousulceration
    ZHANG Suxin1, LI Dan2, CHEN Zhong1, ZHANG Yuwen1*, ZHANG Jinhong2, XU Yanzhi1*
    2016, 37(11):  1285-1288.  doi:10.3969/j.issn.10073205.2016.11.012
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    [
    Abstract ] Objective Toevaluatesalivaryinterleukin ( IL ) -6andtumornecrosisfactor
    ( TNF )
    -αofpatientswithrecurrentaphthousulceration ( RAU ) .Methods Atotalof30patients
    withRAU wereselectedastheresearchsubjects , including18casesofulcerand12casesof
    intermittentperiod.Thrityhealthyvolunteerswereusedasnormalcontrol.TNF-αandIL-6
    levelsweredeterminedbyusingenzyme-linkedimmunosorbentassay.Results Thecontent
    differenceofTNF-αinthe3groupswasstatisticallysignificant , theulcerphaseandtheinterval
    groupwerehigherthanthoseinthecontrolgroup , whiletheintervalgroupwashigherthanthat
    oftheulcergroup , thedifferencewasstatisticallysignificant (
    P <0.05 ) .IntheIL-6content ,
    therewasnosignificantdifferencebetweenthe3groups ( P >0.05 ) .Therewasnocorrelation
    betweenthelevelsofTNF-αandIL-6insalivaofRAUpatients ( r =-0. 090 , P =0. 638 ) .Conclusion
    TNF-αcontentincreasedinsalivamaybeoneofthemainriskfactorsofRAUetiology.
    Observation of the expression of VEGF, flk1, bax and bcl2 in the retina of different types of DR rat model
    DONG Baixia， BAO Yongqin， WANG Zhendong， WANG Jiayao
    2016, 37(11):  1289-1293.  doi:10.3969/j.issn.10073205.2016.11.013
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    [ Abstract ] Objective To observethevariation ofvascularendothelialgrowthfactor
    ( VEGF ), flk-1 , bax , bcl-2expressioninPDRratretinaeatablishedbysymphysialinjectionand
    todefiniteitssuperiority.Methods Sprague-Dawleyratswere madeasdiabeticretinopathy
    modelsbysolitaryinjection withstreptozotocinintraperitoneallyorsymphysialinjection with
    streptozocinintraperitoneally and VEGFintravitreouslyrespectively.Usingfactorialdesign
    empiricalmethod , weobservedthevariationofVEGF , flk-1 , bax , bcl-2expressioninretinasof
    diabeticratsestablished by different methodsbyimmunohistochemistry and measuredthe
    averageopticaldensityvalueofVEGF , flk-1 , bax , bcl-2inretinalganglioncelllayerandinner
    plexiformlayerbyMIAS1998typeimageanalysissystem.Results VEGF , flk-1 , bax , bcl-2were
    expressedineachlayerofretinainbothgroupsexceptthelayerofrodsandcones.Thefrontthree
    targetswereexpressedstrongestintheganglionandnervefiberlayer , whilebcl-2wasexpressed
    thestrongestininnersegmentoftherodsandconeslayer.ThenextstrongestlayerofVEGF expressionwasthe Müllercells whilethenextstrongestlayerofbcl-2expression wasthe
    ganglionand nervefiberlayer.Alltargetsexpressioninsymphysialinjection groups were
    strongerthanthoseinsolitaryinjectiongroups.Bcl-2expressioninganglionandnervefiberlayer
    at8 weeksafter VEGFinjection wasstrongerthanthatat2 weeks.Thestrongestbcl-2
    expression wasininnerplexiform layerat8 weeksinsolitaryinjection groups , whilein
    symphysialinjectiongroupsitwasinganglionandnervefiberlayerat2 weeksafterVEGF
    injection.Conclusion PDRrat modelestablishedbysymphysialinjectioncanrepresentthe
    pathologicalchangesofhumanPDRandhasitsscientificresearchworth.
    Analysis of common deafness genes in 60 patients with cochlear implantation
    ZHANG Xuemei1, ZHAO Ning1, DONG Mei2, LIU Hui1, MAO Yinan3, ZANG Wen1
    2016, 37(11):  1294-1297.  doi:10.3969/j.issn.10073205.2016.11.014
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    [
    Abstract ] Objective Toanalyzetheincidenceofhereditaryhearinglossinpatientswith
    cochlearimplantation.Methods Usingpolymerasechainreactionandrestrictionenzymemethod ,
    themutationsof235delC GJB2 , IVS7-2A>G PDSand mitochondrial12sRNA A1555G DAN
    weredetectedin60patientswithcochlearhearingloss.Results Atotalof33patientswith
    geneticmutationweredetectedin60cochlearimplantationpatients , andthetotalmutationrate
    was55.0% , including : 235delC homozygous mutationin 6 cases ( 10.0% ), 7 cases with
    heterozygous mutation ( 11.7% ); PDS genehomozygousIVS7-2A > G mutationin 6 cases
    (
    10.0% ), heterozygousmutationin12cases ( 20.0% ); DNA mitochondrial12sRNA A1555G
    mutationpositivefor2cases ( 3.3% ) .Conclusion Themaincausesofthediseasearegenetic
    factors , IVS7-2A>GgenePDS , followedbythe235delCgeneGJB2sitemutation.Somepatients
    aremitochondrial12sRNAA1555GDANsitemutation.
    Effect of dexmedetomidine on sedation on  the postoperative sleep status of  type 2 diabetes elderly patients undergoing spinal anesthesia
    ZHAO Tong1， MA Xiaojing2*， ZHAO Jianhui2， LV Zhongli3， SONG Binqiang4， SONG Tieying2
    2016, 37(11):  1298-1301.  doi:10.3969/j.issn.10073205.2016.11.015
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    [
    Abstract ] Objective Toevaluatetheeffectofdexmedetomidineassistedsedationonthe
    postoperativesleepstatusofelderlydiabetespatientsundergoingspinalanesthesia.Methods
    Fortypatientsunderspinalanesthesiaonsaphenousveinligationandstrippingelectivesurgery
    wererandomlydividedintotwogroups ( n =20 ): dexmedetomidinesedationgroup ( groupD ) and
    controlgroup ( groupC ) .Thesleepqualityoflastmonthinallthepatientswereassessedusing
    thePittsburghSleep QualityIndex ( PSQI ) thedaybeforeoperation.Whenlumbarepidural
    anesthesiakeepstable , groupDbegancontinuousintravenousinfusionwithdexmedetomidine0.2
    μ g ·
    kg
    -1 · h -1 ,
    groupCwithsalineinfusion.Bothgroupswerebloodglucosemonitoredatthree
    timepoints : immediatelyafterpuncture ( T 1 ), onehouraftertheoperationstart ( T 2 ), attheend ofoperation ( T 3 ) .OnemonthlaterthesleepqualityofallthepatientswereassessedusingPSQI
    onceagain.Results Therewasnosignificantdifferenceincomparisonofbloodglucoselevels
    betweenthetwogroups , betweentheT 1 -T 3 timepoints , andbetweentheinteractionofgroup · time
    points ( P >0.05 ), andnodifferenceinthePSQIscores (
    P >0.05 ), whilepostoperativePSQI
    scoresinthetwogroupswerelowerthanthatbeforeoperation , andthedexmedetomidinegroup
    waslowerthanthatofthecontrolgroup ( P <0.05 ) .Conclusion Dexmedetomidinegivenspinal
    anesthesiaassistsedationcanimprovepostoperativesleepstatusoftheelderlypatientswithtype
    2diabetetoacertainextent.
    Establishment and application of multiplex realtime fluorescence quantitative PCR assay for detecting various respiratory pathogens
    DI Hongqin, YANG Yonghui, KANG Lifei, AN Xiaoying, LI Xiaoxia, ZHANG Hui
    2016, 37(11):  1302-1306.  doi:10.3969/j.issn.10073205.2016.11.016
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    [
    Abstract ] Objective Todevelopmultiplexreal-timefluorescencequantitativePCR ( MRT-
    PCR ) assayfor detecting variousrespiratory pathogens.Methods MRT-PCR assay was
    establishedwithkindsofrespiratorypathogenicgenes , selectedbasedontheirrelevantreference
    strainsbybioinformaticsanalysis , astargetgenes.Standardproductsandqualitycontrolsamples
    werebuilttotestingandanalysisingthesensitivity , specificityandreproducibilityofthissystem.
    Clinicalsampleswereusedtoevaluating MRT-PCRandsinglefluorescencequantitativePCR.
    Results The MRT-PCR detectionsystem weresuccessfullyestablished whichshowedfine
    specificity.Thedetectionlimitoftheassayswas10copies / μ L.Coefficientofvariationwasfrom
    0. 99%to2. 50% , whichconductedtoassessthereproducibilityoftheassays.Among530clinical
    specimens , thedetectableratesof MRT-PCRandsinglefluorescencequantitativePCR were
    59. 2% ( 314 / 530 ) and61. 1% ( 324 / 530 ), respectively.Conclusion Wedevelopedafast , specific ,
    sensitiveand stable MRT-PCR detection assay , which possessed good clinicalapplication
    prospect.
    Study on the variation and correlation of regulatory T cells in peripheral blood and serum TGFβ and IL10 in patients with gynecological tumor
    YU Danjun1, FAN Jing2, HU Yue1, JIANG Li1, CAO Liyan1, MEN Shuai1
    2016, 37(11):  1307-1311,1316.  doi:10.3969/j.issn.10073205.2016.11.017
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    [
    Abstract ] Objective ToexploretheexpressionofregulatoryTcells ( Treg ) andTcell
    subgroupinperipheralbloodandthevariationandcorrelationoftransforminggrowthfactor-
    β
    ( TGF-
    β ) andinterleukin-10 (
    IL-10 ) cytokinesinseruminpatientswithgynecologicalcancer.
    Methods Ninety-sevencases ( 32caseswithcervicalcancer , 35caseswithendometrialcancer , 30
    caseswithovariancancer ) ofpatientswithgynecologicalcancerand30casesofhealthycontrols
    wereobtainedasresearchobjects.FlowcytometrywasusedtodetectCD4
    + CD25 + Foxp3 +
    and
    CD4
    + CD25 + CD127 low regulatoryTcellsandthedistributionofTcellsubgroupinperipheral
    bloodofallthesubjects.ELISA wasusedtodetectthecontentofTGF-
    β 1 、 TGF- β 2andIL-10 cytokinesinserum.Results Comparedwithhealthycontrolgroup , CD4
    + CD25 +
    Foxp3
    +
    and
    CD4
    + CD25 + CD127 low
    regulatoryTcellsaccountedfortheproportionofCD4
    +
    Tcellsexpression
    inpatientswithgynecologicalcancergroupinperipheralbloodweresignificantlyhigher ( P <
    0.05 ) .TherewasnostatisticalsignificantdifferenceinTcellsubgroupproportion ( P >0.05 ) .
    ThecontentsofTGF-
    β 1andIL-10inserum weresignificantlyhigher (
    P <0.05 ), thecontentof
    TGF-
    β 2wassignificantlylower (
    P <0.05 ) .TheproportionofCD4
    + CD25 + Foxp3 +
    andCD4
    +
    CD25
    + CD127 low regulatoryTcellsaccountedforCD4 + TcellsinperipheralbloodandTGF-
    β 1 、
    TGF-
    β 2andIL-10in serum expressionin patients with gynecologicalcancer group were
    significantlylowerinonemonthaftertheoperation , andafterfourcyclesofchemotherapy ( P <
    0.05 ), CD4
    + 、
    CD8
    + TcellsandCD4 + /
    CD8
    + inonemonthaftertheoperation 、
    CD4
    + 、
    CD19
    + T
    cellsafterfourcyclesofchemotherapyweresignificantlylowerthanthatbeforetheoperation
    (
    P <0.05 ) .TherewaspositivecorrelationbetweenCD4
    + CD25 + Foxp3 + TregandTGF-
    β 1 (
    r s =
    0.220 , P =0.034 ) .TherewerepositivecorrelationbetweenCD4
    + CD25 +
    CD127
    low regulatoryT
    cellsandthecontentofTGF-
    β 1 、 TGF- β 2andIL-10 (
    r s =0.284 , 0.385 , 0.336 , P <0.01 ) .
    Conclusion Theimmunetolerance mechanisminpatients withgynecologycancer mightbe
    relatedtotheratioofCD4
    + CD25 + Foxp3 + TregandCD4 + CD25 + CD127 low
    Tregaccountedtothe
    proportionofCD4
    +
    Tcells , andtothecontentofTGF-
    β 1 、
    TGF-
    β 2andIL-10cytokines.
    The timeeffect analysis on the allocation of the health human resource in Hebei province
    YANG Fangli1, GUO Jianhua2*, CHAI Xuejiao3
    2016, 37(11):  1312-1316.  doi:10.3969/j.issn.10073205.2016.11.018
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    [ Abstract ] Objective Toanalyzetheeconomiceffect , humancapitaleffect , andthe
    situationofthesolutionaboutthehealthdemandfromthehealthhumanresouceinputinHebei
    provinceinordertoprovidetheevidencefortherationalallocationofthehealthhumanresouce.
    Methods Causalrelationshipbetweenthehealthstaffandgrossdomesticproduct , population ,
    employees , thenumberofoutpatientsandthenumberofinpatientswasanalyzedbyGrangertest
    basedonthedatafrom 1994to2014 , respectively.Results ADFtestshowedthatabove-
    mentioned6timeseriestransformedwereallthestablesequences.Then , Johansencointegration
    testshowedtherewasalong-termco-integrationrelationshipamongthehealthstaffandgross
    domesticproduct , employees , thenumberofoutpatientsandthenumberofinpatients.Further ,
    GrangercausaltestfoundthatthehealthstaffwastheGrangercauseforgrossdomesticproduct
    (
    P =11.591 , P =0.003 ), andfortheemployees ( F =9.672 , P =0.010 ), respectively.The contributionrateofhealthstaffwas104. 19%and90. 15%forgrossdomesticproductandforthe
    employees , respectively.Thenumberofoutpatients ( F =10.342 , P =0.005 ) andthenumberof
    inpatients ( P =11.233 , P =0.003 ) wastheGrangercauseforthehealthstaff , respectively.
    Whenthenumberofoutpatientsandthenumberofinpatientsincreases1% , thenumberof
    healthstaffwillincrease10.26% , 0.11%.Conclusion Thesignificanteconomiceffectand
    humanresourceeffectwereobtainedfromthehealthhumanresourceinput , whiletheeffecton
    thesolutionofthehealthdemandsofresidentswasnotconfirmed.Thenumberofhealthhuman
    resourceincreasedwiththeincreasingofhealthdemandofresidents , andthatdidnotincrease
    withtheincreasingofgrossdomesticproductandthenumberofemployees